Rh3DG113300

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002962
Physical Location & Seq
Reverse (-)
9358972 .. 9361064
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG113300.1

Sequence Viewer

Length: 1491 bp
ATGAACATGGTGGAGAATGACAGAAATGAGGTGGAGAGCTCTCGGGAGAGCAGTGAAGCGGAGAAACTAAAGAGAACCAGTTGGGTGGAGAGTCTTGCAGAACTCGGCAGAAACAACTGGAAGAATATTTGGCAGCAAAAAGTGGTTGGGGGTGAAGACAACGACTGTGACGAGGAAAGGAAACGCAGATCATATGATCGTGACCTTTTCTCGAGATTTTTGGTTGGAGTACCATGGTCAGATACCAAATTATTCTCTCAGCTAGCCTTCTTGTGCAACTTGGCTTATGTGATACCAGAGATCAGGGCCACGGATTTAAAGAGATACTATGGCCTACAATTTGTAACATCTTCATTAGATAAGCAAGCAAAGGCAGATAGAATTGTGAGTATCAAATCAAAACGTGATCAGGACTCTACTGCAGTGAATTCTATGGCCTCAGCTGCAGCTTATCTCCGCTCAGTAAGCAAATCACAGCAGGAGGTTGCTGGAGAGAATTCACGCAGAAATGGAGGGAGCTCACCCCAAAATCATAAGTCAGAGATAGATGCTTCCCTTACAGCATCAACAATGACGTTGAGGCTCACAGCAGGTAATGAGGAAAACCAAGGAGCAGCAAAGGATATTCAGTCACTGCACTCCTTACCTTGTGAGTGGTTTGTTTGTGATGAGCCCACTACATATACTCGTTTCTTTGTGATTCGGGGTATAGACTCCCTTGCATCCTGGCAGGCATATTTCTTATTTGAACCCATTGAATTTGAGGGAACTAGTGTACTTGTTCACCGAGGAATTTATGAACTGGCAAAGGAAATTTACCAGCAGTTCAAACCCAAAATAATTGACCACTTGGCAAGATATGGGGAGCAAGCGAAGGTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTTTCTGTTATAGTCCACTTGATGCTACTGACTAGGAAGCTCGTCAAACCCTCAACTCTTCTGCCTGTTGTCACATTTGGCTCCCCATATGTGTTCTGTGGAGGCCAGAAAATATTTGAGCAACTAGGATTTGATGAGTATGATCACATCCGTTGTGTTATGCTGCACAGAGATATTGTCCCTAGATCTTTCTCCTCTAACTACCCTAATCATGTTGCAGAAGTTCTTAAGCGTTTACACAGTTCATTTGAGTCACACCCTTGTCTAACAAAGAATAAGATATTGTACTCTCCTATGGGTAGACTATTTATCGTCCAACCTGATGAAAAGTCATCTCCTCCACACCCTCTACTCCCTTCGGGGAGCTCTCTCTATGCTTTGGACATTACCCAATGTGGATTCTCCTCGAATGTCTTTAGGGCCTTTTTGAATTCCCCACATCCACTAGAAACACTAAGTGATCCTACAGCGTATGGTTCGGAAGGTACAATTCTAAGGGACCATGACTCGAGCAACTATCTAAAGGCTGTGAATGGGGTCCTAATTAAGGCTATACAAAGGGAAGGTTGTCAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

496

Amino Acids

55.92

Weight (kDa)

6.71

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 232 - 368 5.8e-23 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 581
AccBSI CCGCTC 1 cut(s) 459
AccI GTMKAC 1 cut(s) 1210
AciI CCGC 2 cut(s) 59, 457
AclWI GGATC 1 cut(s) 1364
AcoI YGGCCR 1 cut(s) 889
AcsI RAATTY 6 cut(s) 427, 496, 758, 792, 813, 1339
AdeI CACNNNGTG 1 cut(s) 1367
AfaI GTAC 4 cut(s) 231, 777, 1196, 1396
AfiI CCNNNNNNNGG 1 cut(s) 1456
AflII CTTAAG 1 cut(s) 1136
AgsI TTSAA 5 cut(s) 749, 758, 829, 881, 1339
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 1 cut(s) 725
AluBI AGCT 8 cut(s) 39, 262, 443, 449, 519, 949, 1275, 1488
AluI AGCT 8 cut(s) 39, 262, 443, 449, 519, 949, 1275, 1488
Alw21I GWGCWC 3 cut(s) 41, 521, 1277
AlwI GGATC 1 cut(s) 1364
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 3 cut(s) 42, 211, 1417
AoxI GGCC 6 cut(s) 306, 331, 435, 889, 1012, 1329
ApeKI GCWGC 5 cut(s) 133, 443, 446, 614, 1072
ApoI RAATTY 6 cut(s) 427, 496, 758, 792, 813, 1339
Asp700I GAANNNNTTC 1 cut(s) 910
AspS9I GGNCC 4 cut(s) 306, 1329, 1408, 1447
AsuHPI GGTGA 3 cut(s) 164, 513, 776
AsuNHI GCTAGC 1 cut(s) 262
AvaI CYCGRG 3 cut(s) 42, 211, 1417
AvaII GGWCC 2 cut(s) 1408, 1447
BalI TGGCCA 1 cut(s) 891
BanII GRGCYC 4 cut(s) 41, 521, 675, 1277
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 3 cut(s) 41, 521, 1277
BbvCI CCTCAGC 1 cut(s) 439
BbvI GCAGC 5 cut(s) 145, 430, 458, 626, 1059
BciT130I CCWGG 1 cut(s) 727
BclI TGATCA 2 cut(s) 406, 1051
BcuI ACTAGT 1 cut(s) 770
BfaI CTAG 7 cut(s) 263, 771, 942, 1034, 1092, 1355, 1489
BfmI CTRYAG 3 cut(s) 420, 444, 1374
BfrI CTTAAG 1 cut(s) 1136
BfuAI ACCTGC 1 cut(s) 581
BglII AGATCT 1 cut(s) 1094
BisI GCNGC 5 cut(s) 134, 444, 447, 615, 1073
BlsI GCNGC 5 cut(s) 135, 445, 448, 616, 1074
Bme1390I CCNGG 1 cut(s) 727
Bme18I GGWCC 2 cut(s) 1408, 1447
BmeT110I CYCGRG 3 cut(s) 42, 211, 1417
BmgT120I GGNCC 4 cut(s) 306, 1329, 1408, 1447
BmiI GGNNCC 3 cut(s) 991, 1409, 1448
BmrFI CCNGG 1 cut(s) 727
BmsI GCATC 4 cut(s) 538, 572, 731, 921
BmtI GCTAGC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 439
BsaJI CCNNGG 4 cut(s) 233, 309, 607, 787
Bsc4I CCNNNNNNNGG 1 cut(s) 1456
Bse1I ACTGG 4 cut(s) 78, 122, 807, 892
BseBI CCWGG 1 cut(s) 727
BseDI CCNNGG 4 cut(s) 233, 309, 607, 787
BseGI GGATG 3 cut(s) 722, 1056, 1348
BseLI CCNNNNNNNGG 1 cut(s) 1456
BseMII CTCAG 3 cut(s) 272, 453, 474
BseNI ACTGG 4 cut(s) 78, 122, 807, 892
BseRI GAGGAG 3 cut(s) 1093, 1236, 1303
BseXI GCAGC 5 cut(s) 145, 430, 458, 626, 1059
BsgI GTGCAG 2 cut(s) 620, 1058
BshFI GGCC 6 cut(s) 308, 333, 437, 891, 1014, 1331
BsiHKAI GWGCWC 3 cut(s) 41, 521, 1277
BsiHKCI CYCGRG 3 cut(s) 42, 211, 1417
BslFI GGGAC 2 cut(s) 1073, 1421
BslI CCNNNNNNNGG 1 cut(s) 1456
BsmFI GGGAC 2 cut(s) 1073, 1421
BsnI GGCC 6 cut(s) 308, 333, 437, 891, 1014, 1331
BsoBI CYCGRG 3 cut(s) 42, 211, 1417
Bsp1286I GDGCHC 4 cut(s) 41, 521, 675, 1277
Bsp143I GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 2 cut(s) 59, 457
BspANI GGCC 6 cut(s) 308, 333, 437, 891, 1014, 1331
BspCNI CTCAG 3 cut(s) 271, 452, 473
BspLI GGNNCC 3 cut(s) 991, 1409, 1448
BspMAI CTGCAG 2 cut(s) 424, 448
BspMI ACCTGC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 266
BspPI GGATC 1 cut(s) 1364
BspTI CTTAAG 1 cut(s) 1136
BsrBI CCGCTC 1 cut(s) 459
BsrI ACTGG 4 cut(s) 78, 122, 807, 892
BssECI CCNNGG 4 cut(s) 233, 309, 607, 787
BssMI GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
BssT1I CCWWGG 2 cut(s) 233, 607
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 2 cut(s) 167, 1151
Bst6I CTCTTC 1 cut(s) 972
BstAFI CTTAAG 1 cut(s) 1136
BstC8I GCNNGC 4 cut(s) 264, 366, 732, 870
BstDEI CTNAG 5 cut(s) 258, 439, 460, 1364, 1403
BstDSI CCRYGG 2 cut(s) 233, 309
BstENI CCTNNNNNAGG 1 cut(s) 1454
BstF5I GGATG 3 cut(s) 722, 1056, 1348
BstKTI GATC 7 cut(s) 191, 199, 303, 409, 1054, 1097, 1372
BstMBI GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
BstMWI GCNNNNNNNGC 2 cut(s) 443, 465
BstNI CCWGG 1 cut(s) 727
BstSCI CCNGG 1 cut(s) 725
BstSFI CTRYAG 3 cut(s) 420, 444, 1374
BstV1I GCAGC 5 cut(s) 145, 430, 458, 626, 1059
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 1094
BstXI CCANNNNNNTGG 2 cut(s) 85, 899
BstYI RGATCY 1 cut(s) 1094
BsuRI GGCC 6 cut(s) 308, 333, 437, 891, 1014, 1331
BtgI CCRYGG 2 cut(s) 233, 309
BtsCI GGATG 3 cut(s) 722, 1056, 1348
BtsI GCAGTG 3 cut(s) 58, 429, 632
BtsIMutI CAGTG 3 cut(s) 58, 429, 632
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 4 cut(s) 264, 366, 732, 870
CaiI CAGNNNCTG 1 cut(s) 634
Cfr13I GGNCC 4 cut(s) 306, 1329, 1408, 1447
Csp6I GTAC 4 cut(s) 230, 776, 1195, 1395
CviAII CATG 4 cut(s) 7, 234, 1121, 1412
CviQI GTAC 4 cut(s) 230, 776, 1195, 1395
DdeI CTNAG 5 cut(s) 258, 439, 460, 1364, 1403
DpnI GATC 7 cut(s) 190, 198, 302, 408, 1053, 1096, 1371
DpnII GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
DraI TTTAAA 1 cut(s) 318
DraIII CACNNNGTG 1 cut(s) 1367
EaeI YGGCCR 1 cut(s) 889
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Ecl136II GAGCTC 3 cut(s) 39, 519, 1275
Eco130I CCWWGG 2 cut(s) 233, 607
Eco24I GRGCYC 4 cut(s) 41, 521, 675, 1277
Eco47I GGWCC 2 cut(s) 1408, 1447
Eco53kI GAGCTC 3 cut(s) 39, 519, 1275
Eco88I CYCGRG 3 cut(s) 42, 211, 1417
EcoICRI GAGCTC 3 cut(s) 39, 519, 1275
EcoNI CCTNNNNNAGG 1 cut(s) 1454
EcoO109I RGGNCCY 2 cut(s) 1329, 1447
EcoRI GAATTC 3 cut(s) 427, 496, 1339
EcoRII CCWGG 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 233, 607
EcoT38I GRGCYC 4 cut(s) 41, 521, 675, 1277
ErhI CCWWGG 2 cut(s) 233, 607
FaeI CATG 4 cut(s) 10, 237, 1124, 1415
FaqI GGGAC 2 cut(s) 1073, 1421
FatI CATG 4 cut(s) 6, 233, 1120, 1411
FauNDI CATATG 2 cut(s) 193, 997
FbaI TGATCA 2 cut(s) 406, 1051
FblI GTMKAC 1 cut(s) 1210
Fnu4HI GCNGC 5 cut(s) 134, 444, 447, 615, 1073
FokI GGATG 3 cut(s) 709, 1043, 1335
FriOI GRGCYC 4 cut(s) 41, 521, 675, 1277
Fsp4HI GCNGC 5 cut(s) 134, 444, 447, 615, 1073
FspBI CTAG 7 cut(s) 263, 771, 942, 1034, 1092, 1355, 1489
GluI GCNGC 5 cut(s) 134, 444, 447, 615, 1073
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 6 cut(s) 308, 333, 437, 891, 1014, 1331
Hin1II CATG 4 cut(s) 10, 237, 1124, 1415
HinfI GANTC 7 cut(s) 91, 413, 700, 713, 1160, 1308, 1415
HphI GGTGA 3 cut(s) 164, 513, 776
Hpy166II GTNNAC 5 cut(s) 776, 784, 925, 1145, 1211
Hpy188I TCNGA 3 cut(s) 241, 541, 1390
Hpy188III TCNNGA 6 cut(s) 44, 200, 211, 213, 410, 1481
Hpy8I GTNNAC 5 cut(s) 776, 784, 925, 1145, 1211
HpyAV CCTTC 5 cut(s) 277, 868, 1275, 1385, 1466
HpyCH4III ACNGT 2 cut(s) 167, 1151
HpyCH4IV ACGT 2 cut(s) 403, 575
HpyCH4V TGCA 8 cut(s) 98, 276, 422, 446, 637, 722, 1075, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 465
HpyF3I CTNAG 5 cut(s) 258, 439, 460, 1364, 1403
HpySE526I ACGT 2 cut(s) 403, 575
Hsp92II CATG 4 cut(s) 10, 237, 1124, 1415
Ksp22I TGATCA 2 cut(s) 406, 1051
Kzo9I GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
LmnI GCTCC 5 cut(s) 516, 611, 865, 995, 1272
Lsp1109I GCAGC 5 cut(s) 145, 430, 458, 626, 1059
LweI GCATC 4 cut(s) 538, 572, 731, 921
MaeI CTAG 7 cut(s) 263, 771, 942, 1034, 1092, 1355, 1489
MaeII ACGT 2 cut(s) 403, 575
MaeIII GTNAC 6 cut(s) 167, 200, 343, 630, 979, 1161
MalI GATC 7 cut(s) 190, 198, 302, 408, 1053, 1096, 1371
MbiI CCGCTC 1 cut(s) 459
MboI GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
MboII GAAGA 4 cut(s) 133, 167, 342, 959
MflI RGATCY 1 cut(s) 1094
MhlI GDGCHC 4 cut(s) 41, 521, 675, 1277
MlsI TGGCCA 1 cut(s) 891
MluNI TGGCCA 1 cut(s) 891
MlyI GAGTC 5 cut(s) 100, 407, 707, 1169, 1409
MmeI TCCRAC 2 cut(s) 205, 1249
Mox20I TGGCCA 1 cut(s) 891
MroXI GAANNNNTTC 1 cut(s) 910
MscI TGGCCA 1 cut(s) 891
MseI TTAA 3 cut(s) 317, 1137, 1455
Msp20I TGGCCA 1 cut(s) 891
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 1 cut(s) 1136
MspR9I CCNGG 1 cut(s) 727
MvaI CCWGG 1 cut(s) 727
MwoI GCNNNNNNNGC 2 cut(s) 443, 465
NcoI CCATGG 1 cut(s) 233
NdeI CATATG 2 cut(s) 193, 997
NdeII GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
NheI GCTAGC 1 cut(s) 262
NlaIII CATG 4 cut(s) 10, 237, 1124, 1415
NlaIV GGNNCC 3 cut(s) 991, 1409, 1448
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 5 cut(s) 167, 200, 630, 979, 1161
PaeR7I CTCGAG 2 cut(s) 211, 1417
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 910
PfeI GAWTC 2 cut(s) 700, 1308
PkrI GCNGC 5 cut(s) 135, 445, 448, 616, 1074
PleI GAGTC 5 cut(s) 99, 407, 707, 1168, 1409
PpsI GAGTC 5 cut(s) 99, 407, 707, 1168, 1409
PpuMI RGGWCCY 1 cut(s) 1447
Psp124BI GAGCTC 3 cut(s) 41, 521, 1277
Psp5II RGGWCCY 1 cut(s) 1447
Psp6I CCWGG 1 cut(s) 725
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 3 cut(s) 991, 1409, 1448
PspPI GGNCC 4 cut(s) 306, 1329, 1408, 1447
PspPPI RGGWCCY 1 cut(s) 1447
PspXI VCTCGAGB 1 cut(s) 1417
PstI CTGCAG 2 cut(s) 424, 448
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 1094
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 4 cut(s) 231, 777, 1196, 1396
RsaNI GTAC 4 cut(s) 230, 776, 1195, 1395
SacI GAGCTC 3 cut(s) 41, 521, 1277
SaqAI TTAA 3 cut(s) 317, 1137, 1455
SatI GCNGC 5 cut(s) 134, 444, 447, 615, 1073
Sau3AI GATC 7 cut(s) 188, 196, 300, 406, 1051, 1094, 1369
Sau96I GGNCC 4 cut(s) 306, 1329, 1408, 1447
SchI GAGTC 5 cut(s) 100, 407, 707, 1169, 1409
ScrFI CCNGG 1 cut(s) 727
SduI GDGCHC 4 cut(s) 41, 521, 675, 1277
SfaNI GCATC 4 cut(s) 538, 572, 731, 921
SfcI CTRYAG 3 cut(s) 420, 444, 1374
Sfr274I CTCGAG 2 cut(s) 211, 1417
SinI GGWCC 2 cut(s) 1408, 1447
SlaI CTCGAG 2 cut(s) 211, 1417
SmlI CTYRAG 3 cut(s) 211, 1136, 1417
SmoI CTYRAG 3 cut(s) 211, 1136, 1417
SpeI ACTAGT 1 cut(s) 770
SsiI CCGC 2 cut(s) 59, 457
SspI AATATT 2 cut(s) 127, 1023
SspMI CTAG 7 cut(s) 263, 771, 942, 1034, 1092, 1355, 1489
SstI GAGCTC 3 cut(s) 41, 521, 1277
StyD4I CCNGG 1 cut(s) 725
StyI CCWWGG 2 cut(s) 233, 607
TaaI ACNGT 2 cut(s) 167, 1151
TaiI ACGT 2 cut(s) 406, 578
TaqI TCGA 3 cut(s) 212, 1316, 1418
TatI WGTACW 2 cut(s) 775, 1194
TfiI GAWTC 2 cut(s) 700, 1308
Tru1I TTAA 3 cut(s) 317, 1137, 1455
Tru9I TTAA 3 cut(s) 317, 1137, 1455
TscAI CASTG 3 cut(s) 58, 429, 639
TseFI GTSAC 5 cut(s) 167, 200, 630, 979, 1161
TseI GCWGC 5 cut(s) 133, 443, 446, 614, 1072
Tsp45I GTSAC 5 cut(s) 167, 200, 630, 979, 1161
TspDTI ATGAA 5 cut(s) 17, 342, 813, 1143, 1248
TspGWI ACGGA 2 cut(s) 326, 1049
TspRI CASTG 3 cut(s) 58, 429, 639
Vha464I CTTAAG 1 cut(s) 1136
VpaK11BI GGWCC 2 cut(s) 1408, 1447
XagI CCTNNNNNAGG 1 cut(s) 1454
XapI RAATTY 6 cut(s) 427, 496, 758, 792, 813, 1339
XhoI CTCGAG 2 cut(s) 211, 1417
XmiI GTMKAC 1 cut(s) 1210
XmnI GAANNNNTTC 1 cut(s) 910
XspI CTAG 7 cut(s) 263, 771, 942, 1034, 1092, 1355, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.