RchiOBHm_Chr3g0461341

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002962
Physical Location & Seq
Reverse (-)
9415825 .. 9418048
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42776

Sequence Viewer

Length: 1884 bp
ATGGCATTGACTTCATTAGCTATTCCCAGCTCTCTGGCTTCATCAACAAAATATATCCTCAAAGAGTACAATGACCTTCACCAGTCACACTCTAGCAGAGACCTATGCAATCTTGCTAGTATCCAGGGAAGGTTTTCTTCAGCCGATAACCAGATTTGTTATTGCATCAACCGTAACCATGCAACATCAACAGAACCAGAACCAAAGAAGAGTCCTTTCGTGCGAATTTCCTCGTTTCAAAGTCGGGGTTCTATCATTCCAAATTTGCTACGATCATATTCATTTGATCCAAACTCGAGTAAGCATATGAACATAGTGGAGAATGACAGAAATGAGGTGGAGAGCTCTCGGGAGAGCAGTGAAGCGGAGGAACTAAAGAGAACCAGTTGGGTGGAGAGTCTTGCGGAACTCGGCAGAAACAACTGGAAGAATATTTGGCAGCAAAAAGTGGTTGGGGGTGAAGACAACGACTGTGACGAGGAAAGGAAACGCAGATCATATGATCGTGACCTTTTCTCGAGATTTTTGGTTAGAGTACCATGGTCAGATACCAAATTATTCTCTCAGCTAGCCTTCTTGTGCAACTTGGCTTATGTGATACCAGAGATCAGGGCCACGGATTTAAAGAGATACTATGGCCTACAATTTGTAACATCTTCATTAGATAAGCAAGCAAAGGCAGATAGAATTGTGAGTATCAAATCAAAACGTGATCAGGACTCTACTGCAGTGAATTCTATGGCCTCAGCTGCAGCTTATCTCCGCTCAGTAAGCAAATCACAGCAGGAGGTTGCTGGAGAGAATTCACGCAGAAATGGAGGGAGCTCACCCCAAAATCATAAGTCAGAGATAGATGCTTCCCTTACAGCATCAACAATGACGTTGAGGCTCACAGCAGGTAATGAGGAAAAGCAAGGAGCAGCAAAGGATATTCAGTCACTGCACTCCTTACCTTGTGAGTGGTTTGTTTGTGATGATCCCACTACATATACTCGTTTCTTTGTGATTCAGGGTATAGACTCCCTTGCATCCTGGCAGGCATATTTCTTATTTGAACCCATTGAATTTGAGGTAGGCATGGATAATATACTAAGATACAAAGTCTCCAGTGCAAGGAAAGAAAATGAGAAATTAAACGTAACTGATATCCTCATGCAGGGAACTAGTGTACTTGTTCACCGAGGAATTTATGAACTGGCAAAGGAAATTTACCAGCAGTTCAAACCCAAAATAATTTACCACTTGGCAAGATATGGGGAGCAAGCGAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTTTCTGTTATAGTCCACTTGATGCTACTGACTAGGAAGCTCGTCAAACCCTCAACTCTTCTGCCTGTTGTCACATTTGGCTCCCCATATGTGTTCTGTGGAGGCCAGAAAATATTTGAGCAACTAGGATTTGATGAGTATGATCACATCCGTTGTGTTATGCTGCCCAGAGATATTGTCCCTAGATCTTTCTCCTCTAACTACCCTAATCATGTTGCAGAAGTTCTTAAGCATTTACACGGTTCATTTGAGTCACACCCTTGTCTAACAAAGAATAAGATATTGTACTCTCCTATGGGTAGACTATTTATCGTCCAACCTGATGAAAAGTCATCTCCTCCACACCCTCTACTCCCTTCGGGGAGCTCTCTCTATGCTTTGGACATTACCCAATGTGGATTCTCCTCGAATGTCTTGAGGGCCTTTTTGAATTCCCCACATCCACTAGAAACACTAAGTGATCCTACAGCGTATGGTTCGGAAGGTACAATTCTAAGGGACCATGACTCGAGCAACTATCTAAAGGCTGTGAATGGGGTCCTAATTAAGGCTATACAAAAGGAAGGTTGTCAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

627

Amino Acids

70.58

Weight (kDa)

7.88

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 334 - 499 1.3e-18 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 1603
AciI CCGC 3 cut(s) 365, 404, 763
AclWI GGATC 3 cut(s) 281, 971, 1757
AcoI YGGCCR 1 cut(s) 1282
AcsI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1185, 1206, 1732
AcuI CTGAAG 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1760
AfaI GTAC 5 cut(s) 68, 537, 1170, 1589, 1789
AfiI CCNNNNNNNGG 3 cut(s) 1113, 1156, 1849
AflII CTTAAG 1 cut(s) 1529
AgsI TTSAA 6 cut(s) 239, 1055, 1064, 1222, 1274, 1732
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 2 cut(s) 123, 1031
Alw21I GWGCWC 3 cut(s) 347, 827, 1670
Alw26I GTCTC 2 cut(s) 93, 1108
AlwI GGATC 3 cut(s) 281, 971, 1757
AlwNI CAGNNNCTG 1 cut(s) 940
Ama87I CYCGRG 4 cut(s) 295, 348, 517, 1810
AoxI GGCC 6 cut(s) 612, 637, 741, 1282, 1405, 1722
ApeKI GCWGC 5 cut(s) 439, 749, 752, 920, 1465
ApoI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1185, 1206, 1732
Asp700I GAANNNNTTC 2 cut(s) 133, 1303
AspS9I GGNCC 4 cut(s) 612, 1722, 1801, 1840
AsuHPI GGTGA 4 cut(s) 71, 470, 819, 1169
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 4 cut(s) 295, 348, 517, 1810
AvaII GGWCC 2 cut(s) 1801, 1840
BalI TGGCCA 1 cut(s) 1284
BanII GRGCYC 3 cut(s) 347, 827, 1670
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 3 cut(s) 347, 827, 1670
BbvCI CCTCAGC 1 cut(s) 745
BbvI GCAGC 5 cut(s) 451, 736, 764, 932, 1452
BciT130I CCWGG 2 cut(s) 125, 1033
BciVI GTATCC 1 cut(s) 131
BclI TGATCA 2 cut(s) 712, 1444
BcoDI GTCTC 2 cut(s) 93, 1108
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 9 cut(s) 93, 117, 569, 1164, 1335, 1427, 1485, 1748, 1882
BfmI CTRYAG 3 cut(s) 726, 750, 1767
BfrI CTTAAG 1 cut(s) 1529
BfuAI ACCTGC 1 cut(s) 887
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 1487
BisI GCNGC 5 cut(s) 440, 750, 753, 921, 1466
BlsI GCNGC 5 cut(s) 441, 751, 754, 922, 1467
Bme1390I CCNGG 2 cut(s) 125, 1033
Bme18I GGWCC 2 cut(s) 1801, 1840
BmeT110I CYCGRG 4 cut(s) 295, 348, 517, 1810
BmgT120I GGNCC 4 cut(s) 612, 1722, 1801, 1840
BmiI GGNNCC 3 cut(s) 1384, 1802, 1841
BmrFI CCNGG 2 cut(s) 125, 1033
BmsI GCATC 5 cut(s) 174, 844, 878, 1037, 1314
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 816, 1090
Bpu10I CCTNAGC 1 cut(s) 745
BpuEI CTTGAG 1 cut(s) 1738
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 124, 539, 615, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 1113, 1156, 1849
Bse1I ACTGG 6 cut(s) 82, 384, 428, 1107, 1200, 1285
BseBI CCWGG 2 cut(s) 125, 1033
BseDI CCNNGG 4 cut(s) 124, 539, 615, 1180
BseGI GGATG 3 cut(s) 1028, 1449, 1741
BseLI CCNNNNNNNGG 3 cut(s) 1113, 1156, 1849
BseMII CTCAG 3 cut(s) 578, 759, 780
BseNI ACTGG 6 cut(s) 82, 384, 428, 1107, 1200, 1285
BseRI GAGGAG 3 cut(s) 1486, 1629, 1696
BseXI GCAGC 5 cut(s) 451, 736, 764, 932, 1452
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 926
BshFI GGCC 6 cut(s) 614, 639, 743, 1284, 1407, 1724
BsiHKAI GWGCWC 3 cut(s) 347, 827, 1670
BsiHKCI CYCGRG 4 cut(s) 295, 348, 517, 1810
BslFI GGGAC 2 cut(s) 1466, 1814
BslI CCNNNNNNNGG 3 cut(s) 1113, 1156, 1849
BsmAI GTCTC 2 cut(s) 93, 1108
BsmFI GGGAC 2 cut(s) 1466, 1814
BsnI GGCC 6 cut(s) 614, 639, 743, 1284, 1407, 1724
Bso31I GGTCTC 1 cut(s) 93
BsoBI CYCGRG 4 cut(s) 295, 348, 517, 1810
Bsp1286I GDGCHC 3 cut(s) 347, 827, 1670
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 3 cut(s) 365, 404, 763
BspANI GGCC 6 cut(s) 614, 639, 743, 1284, 1407, 1724
BspCNI CTCAG 3 cut(s) 577, 758, 779
BspLI GGNNCC 3 cut(s) 1384, 1802, 1841
BspMAI CTGCAG 2 cut(s) 730, 754
BspMI ACCTGC 1 cut(s) 887
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 3 cut(s) 281, 971, 1757
BspTI CTTAAG 1 cut(s) 1529
BspTNI GGTCTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 765
BsrI ACTGG 6 cut(s) 82, 384, 428, 1107, 1200, 1285
BssECI CCNNGG 4 cut(s) 124, 539, 615, 1180
BssT1I CCWWGG 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 125, 1033
Bst4CI ACNGT 3 cut(s) 173, 473, 1544
Bst6I CTCTTC 2 cut(s) 203, 1365
BstAFI CTTAAG 1 cut(s) 1529
BstC8I GCNNGC 4 cut(s) 570, 672, 1038, 1263
BstDEI CTNAG 6 cut(s) 564, 745, 766, 1091, 1757, 1796
BstDSI CCRYGG 2 cut(s) 539, 615
BstENI CCTNNNNNAGG 2 cut(s) 1154, 1847
BstF5I GGATG 3 cut(s) 1028, 1449, 1741
BstMAI GTCTC 2 cut(s) 93, 1108
BstMWI GCNNNNNNNGC 3 cut(s) 749, 771, 1267
BstNI CCWGG 2 cut(s) 125, 1033
BstSCI CCNGG 2 cut(s) 123, 1031
BstSFI CTRYAG 3 cut(s) 726, 750, 1767
BstV1I GCAGC 5 cut(s) 451, 736, 764, 932, 1452
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 1487
BstXI CCANNNNNNTGG 3 cut(s) 34, 391, 1292
BstYI RGATCY 1 cut(s) 1487
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 6 cut(s) 614, 639, 743, 1284, 1407, 1724
BtgI CCRYGG 2 cut(s) 539, 615
BtsCI GGATG 3 cut(s) 1028, 1449, 1741
BtsI GCAGTG 3 cut(s) 364, 735, 938
BtsIMutI CAGTG 4 cut(s) 364, 735, 938, 1114
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 4 cut(s) 570, 672, 1038, 1263
CaiI CAGNNNCTG 1 cut(s) 940
Cfr13I GGNCC 4 cut(s) 612, 1722, 1801, 1840
Csp6I GTAC 5 cut(s) 67, 536, 1169, 1588, 1788
CviAII CATG 6 cut(s) 179, 540, 1078, 1153, 1514, 1805
CviQI GTAC 5 cut(s) 67, 536, 1169, 1588, 1788
DdeI CTNAG 6 cut(s) 564, 745, 766, 1091, 1757, 1796
DraI TTTAAA 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 1760
EaeI YGGCCR 1 cut(s) 1282
Eam1104I CTCTTC 2 cut(s) 203, 1365
EarI CTCTTC 2 cut(s) 203, 1365
Ecl136II GAGCTC 3 cut(s) 345, 825, 1668
Eco130I CCWWGG 1 cut(s) 539
Eco24I GRGCYC 3 cut(s) 347, 827, 1670
Eco31I GGTCTC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 1147
Eco47I GGWCC 2 cut(s) 1801, 1840
Eco53kI GAGCTC 3 cut(s) 345, 825, 1668
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 4 cut(s) 295, 348, 517, 1810
EcoICRI GAGCTC 3 cut(s) 345, 825, 1668
EcoNI CCTNNNNNAGG 2 cut(s) 1154, 1847
EcoO109I RGGNCCY 2 cut(s) 1722, 1840
EcoRI GAATTC 3 cut(s) 733, 802, 1732
EcoRII CCWGG 2 cut(s) 123, 1031
EcoRV GATATC 1 cut(s) 1147
EcoT14I CCWWGG 1 cut(s) 539
EcoT38I GRGCYC 3 cut(s) 347, 827, 1670
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 6 cut(s) 182, 543, 1081, 1156, 1517, 1808
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 1254, 1286
FaqI GGGAC 2 cut(s) 1466, 1814
FatI CATG 6 cut(s) 178, 539, 1077, 1152, 1513, 1804
FauNDI CATATG 3 cut(s) 306, 499, 1390
FbaI TGATCA 2 cut(s) 712, 1444
FblI GTMKAC 1 cut(s) 1603
Fnu4HI GCNGC 5 cut(s) 440, 750, 753, 921, 1466
FokI GGATG 3 cut(s) 1015, 1436, 1728
FriOI GRGCYC 3 cut(s) 347, 827, 1670
Fsp4HI GCNGC 5 cut(s) 440, 750, 753, 921, 1466
FspBI CTAG 9 cut(s) 93, 117, 569, 1164, 1335, 1427, 1485, 1748, 1882
GluI GCNGC 5 cut(s) 440, 750, 753, 921, 1466
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 2 cut(s) 816, 1090
HaeIII GGCC 6 cut(s) 614, 639, 743, 1284, 1407, 1724
Hin1II CATG 6 cut(s) 182, 543, 1081, 1156, 1517, 1808
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 8 cut(s) 211, 397, 719, 1006, 1019, 1553, 1701, 1808
HphI GGTGA 4 cut(s) 71, 470, 819, 1169
Hpy166II GTNNAC 4 cut(s) 1169, 1177, 1318, 1604
Hpy188I TCNGA 3 cut(s) 547, 847, 1783
Hpy188III TCNNGA 7 cut(s) 350, 506, 517, 519, 716, 1717, 1874
Hpy8I GTNNAC 4 cut(s) 1169, 1177, 1318, 1604
HpyAV CCTTC 6 cut(s) 86, 123, 583, 1668, 1778, 1859
HpyCH4III ACNGT 3 cut(s) 173, 473, 1544
HpyCH4IV ACGT 3 cut(s) 709, 881, 1137
HpyF10VI GCNNNNNNNGC 3 cut(s) 749, 771, 1267
HpyF3I CTNAG 6 cut(s) 564, 745, 766, 1091, 1757, 1796
HpySE526I ACGT 3 cut(s) 709, 881, 1137
Hsp92II CATG 6 cut(s) 182, 543, 1081, 1156, 1517, 1808
Ksp22I TGATCA 2 cut(s) 712, 1444
LmnI GCTCC 5 cut(s) 822, 917, 1258, 1388, 1665
Lsp1109I GCAGC 5 cut(s) 451, 736, 764, 932, 1452
LweI GCATC 5 cut(s) 174, 844, 878, 1037, 1314
MaeI CTAG 9 cut(s) 93, 117, 569, 1164, 1335, 1427, 1485, 1748, 1882
MaeII ACGT 3 cut(s) 709, 881, 1137
MaeIII GTNAC 9 cut(s) 84, 173, 473, 506, 649, 936, 1138, 1372, 1554
MbiI CCGCTC 1 cut(s) 765
MboII GAAGA 6 cut(s) 129, 220, 439, 473, 648, 1352
MflI RGATCY 1 cut(s) 1487
MhlI GDGCHC 3 cut(s) 347, 827, 1670
MlsI TGGCCA 1 cut(s) 1284
MluNI TGGCCA 1 cut(s) 1284
MlyI GAGTC 6 cut(s) 220, 406, 713, 1013, 1562, 1802
MmeI TCCRAC 1 cut(s) 1642
Mox20I TGGCCA 1 cut(s) 1284
MroXI GAANNNNTTC 2 cut(s) 133, 1303
MscI TGGCCA 1 cut(s) 1284
MseI TTAA 4 cut(s) 623, 1133, 1530, 1848
Msp20I TGGCCA 1 cut(s) 1284
MspA1I CMGCKG 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 1529
MspR9I CCNGG 2 cut(s) 125, 1033
MvaI CCWGG 2 cut(s) 125, 1033
MwoI GCNNNNNNNGC 3 cut(s) 749, 771, 1267
NcoI CCATGG 1 cut(s) 539
NdeI CATATG 3 cut(s) 306, 499, 1390
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 6 cut(s) 182, 543, 1081, 1156, 1517, 1808
NlaIV GGNNCC 3 cut(s) 1384, 1802, 1841
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 6 cut(s) 84, 473, 506, 936, 1372, 1554
PaeR7I CTCGAG 3 cut(s) 295, 517, 1810
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdmI GAANNNNTTC 2 cut(s) 133, 1303
PfeI GAWTC 2 cut(s) 1006, 1701
PkrI GCNGC 5 cut(s) 441, 751, 754, 922, 1467
PleI GAGTC 6 cut(s) 219, 405, 713, 1013, 1561, 1802
PpsI GAGTC 6 cut(s) 219, 405, 713, 1013, 1561, 1802
PpuMI RGGWCCY 1 cut(s) 1840
Psp124BI GAGCTC 3 cut(s) 347, 827, 1670
Psp5II RGGWCCY 1 cut(s) 1840
Psp6I CCWGG 2 cut(s) 123, 1031
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 123, 1031
PspN4I GGNNCC 3 cut(s) 1384, 1802, 1841
PspPI GGNCC 4 cut(s) 612, 1722, 1801, 1840
PspPPI RGGWCCY 1 cut(s) 1840
PspXI VCTCGAGB 2 cut(s) 295, 1810
PstI CTGCAG 2 cut(s) 730, 754
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 1487
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 5 cut(s) 68, 537, 1170, 1589, 1789
RsaNI GTAC 5 cut(s) 67, 536, 1169, 1588, 1788
SacI GAGCTC 3 cut(s) 347, 827, 1670
SaqAI TTAA 4 cut(s) 623, 1133, 1530, 1848
SatI GCNGC 5 cut(s) 440, 750, 753, 921, 1466
Sau96I GGNCC 4 cut(s) 612, 1722, 1801, 1840
SchI GAGTC 6 cut(s) 220, 406, 713, 1013, 1562, 1802
ScrFI CCNGG 2 cut(s) 125, 1033
SduI GDGCHC 3 cut(s) 347, 827, 1670
SfaNI GCATC 5 cut(s) 174, 844, 878, 1037, 1314
SfcI CTRYAG 3 cut(s) 726, 750, 1767
Sfr274I CTCGAG 3 cut(s) 295, 517, 1810
SinI GGWCC 2 cut(s) 1801, 1840
SlaI CTCGAG 3 cut(s) 295, 517, 1810
SmlI CTYRAG 5 cut(s) 295, 517, 1529, 1717, 1810
SmoI CTYRAG 5 cut(s) 295, 517, 1529, 1717, 1810
SpeI ACTAGT 1 cut(s) 1163
SsiI CCGC 3 cut(s) 365, 404, 763
SspI AATATT 2 cut(s) 433, 1416
SspMI CTAG 9 cut(s) 93, 117, 569, 1164, 1335, 1427, 1485, 1748, 1882
SstI GAGCTC 3 cut(s) 347, 827, 1670
StyD4I CCNGG 2 cut(s) 123, 1031
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 3 cut(s) 173, 473, 1544
TaiI ACGT 3 cut(s) 712, 884, 1140
TaqI TCGA 4 cut(s) 296, 518, 1709, 1811
TatI WGTACW 3 cut(s) 66, 1168, 1587
TfiI GAWTC 2 cut(s) 1006, 1701
Tru1I TTAA 4 cut(s) 623, 1133, 1530, 1848
Tru9I TTAA 4 cut(s) 623, 1133, 1530, 1848
TscAI CASTG 4 cut(s) 364, 735, 945, 1114
TseFI GTSAC 6 cut(s) 84, 473, 506, 936, 1372, 1554
TseI GCWGC 5 cut(s) 439, 749, 752, 920, 1465
Tsp45I GTSAC 6 cut(s) 84, 473, 506, 936, 1372, 1554
TspDTI ATGAA 7 cut(s) 30, 270, 323, 648, 1206, 1536, 1641
TspGWI ACGGA 2 cut(s) 632, 1442
TspRI CASTG 4 cut(s) 364, 735, 945, 1114
Vha464I CTTAAG 1 cut(s) 1529
VpaK11BI GGWCC 2 cut(s) 1801, 1840
XagI CCTNNNNNAGG 2 cut(s) 1154, 1847
XapI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1185, 1206, 1732
XhoI CTCGAG 3 cut(s) 295, 517, 1810
XmiI GTMKAC 1 cut(s) 1603
XmnI GAANNNNTTC 2 cut(s) 133, 1303
XspI CTAG 9 cut(s) 93, 117, 569, 1164, 1335, 1427, 1485, 1748, 1882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.