MD12G1184000.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002962
Physical Location & Seq
Reverse (-)
26415941 .. 26417776
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1184000.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGAGATACAGATCATACGACCAGGAGTCTTTCTCGAGGTTTTTAGTTAGAGTACCATGGTCAGATACCAAGCTATTTTCTAAGCAAGCCTTCTTGTGCAACATGGCTTGTGTGATACCAGAGATCAAGTTCAACGATGACAGAGTACTTCAAGCAGCAAGCGAAATGGCAAGACAAGAGACGGAAAATGACCTTTGGTCACTCCACCACCCCTTGCCTTGTGAATGGTTTGTTTGTGATGACTCCAGTACGTCTACTCGTTGCTTCGTGATTCAGGAGATGAATGTACATGTTCACAGAGGAACCTATGAAGCGGCACAGCAAATGTACCAGCAATTCATGCCAGATATATTGGATCACTTACATAGAAATGGTGAACTCGCAAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCATTGCTCTTTTAGTCCACATGATGCTCCTCACTAGGCAGCTTGTGAAACCCTCTACTCTTCTGCCGGTTGTCACATTTGGATCACCATTTGTGTTCTGCGGAGGCCACAAGTTACTTGATCAAAAATGGTTGGATGCGAATAATCACATCCATTGTGTGATGATGCATAGAGATGTTGTCCCTAGAGCTTTCTCCTGCAACTACCCCAACCATGTGGCTACACTCCTCAAGCGTTTAAATGGTTCATTTCAGTCACTCCCTTGCCTGATAAAAAAAAATACGTTGTACTCTCCAATCGGCAAACTATTCATTCTTCAACCTGATGAAAGGTTTTCTCCTCCACACCCTCTACTCCCTATGGGGAGTGCCCCCTATGCCTTGGACAACACGCAATCTGGTTTCTCTTCAGATATCTTTAGGGCCTTCTTGAACTCCCCACATCCACTAGAAACCCTTAGTGATCCTACAGCTTACGGTTCAGAAGGTACAATCTTACGTGACCACGATTCAGACAACTATCTAAAGGCTATCAATAAGATCCTACGGAAACACACAAGGATGGTTGTGAGGAAAGTGAGGAAGCAGCGGAATCTCCTGTGGCCGCTGCTCACTTCACCCTCTCCACACCGGTTGAACGATGAAGGTGAAAGTGGTCTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

361

Amino Acids

41.24

Weight (kDa)

8.63

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 92 - 207 1.4e-21 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 254
AciI CCGC 4 cut(s) 314, 519, 1006, 1022
AclWI GGATC 4 cut(s) 363, 508, 875, 952
AcoI YGGCCR 2 cut(s) 400, 1019
AcuI CTGAAG 1 cut(s) 810
AfaI GTAC 7 cut(s) 54, 147, 250, 288, 329, 707, 907
AflIII ACRYGT 1 cut(s) 289
AgeI ACCGGT 1 cut(s) 1047
AgsI TTSAA 6 cut(s) 133, 152, 392, 737, 850, 1054
AhdI GACNNNNNGTC 2 cut(s) 25, 196
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 5 cut(s) 73, 388, 460, 608, 890
AluI AGCT 5 cut(s) 73, 388, 460, 608, 890
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 4 cut(s) 363, 508, 875, 952
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 4 cut(s) 400, 523, 840, 1019
ApeKI GCWGC 4 cut(s) 155, 457, 1003, 1024
AsiGI ACCGGT 1 cut(s) 1047
AspS9I GGNCC 1 cut(s) 840
AsuHPI GGTGA 4 cut(s) 386, 495, 1026, 1076
AvaI CYCGRG 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 402
BbvI GCAGC 4 cut(s) 167, 469, 1011, 1015
BccI CCATC 1 cut(s) 973
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 3 cut(s) 453, 603, 866
BfmI CTRYAG 1 cut(s) 885
BisI GCNGC 6 cut(s) 156, 315, 458, 1004, 1022, 1025
BlsI GCNGC 6 cut(s) 157, 316, 459, 1005, 1023, 1026
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 23
BmeRI GACNNNNNGTC 2 cut(s) 25, 196
BmeT110I CYCGRG 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 840
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 432, 544, 573
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 632
BsaAI YACGTR 1 cut(s) 917
BsaBI GATNNNNATC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 56, 798
BsaWI WCCGGW 1 cut(s) 1047
Bse118I RCCGGY 2 cut(s) 484, 1047
Bse1I ACTGG 2 cut(s) 246, 403
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 10
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 56, 798
BseGI GGATG 4 cut(s) 559, 567, 859, 984
BseJI GATNNNNATC 1 cut(s) 10
BseMI GCAATG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 246, 403
BseRI GAGGAG 3 cut(s) 437, 635, 747
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 4 cut(s) 167, 469, 1011, 1015
BshFI GGCC 4 cut(s) 402, 525, 842, 1021
BshTI ACCGGT 1 cut(s) 1047
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 2 cut(s) 485, 1048
BslFI GGGAC 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 4 cut(s) 402, 525, 842, 1021
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 790
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 4 cut(s) 314, 519, 1006, 1022
BspANI GGCC 4 cut(s) 402, 525, 842, 1021
BspLI GGNNCC 1 cut(s) 304
BspPI GGATC 4 cut(s) 363, 508, 875, 952
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 2 cut(s) 484, 1047
BsrGI TGTACA 1 cut(s) 286
BsrI ACTGG 2 cut(s) 246, 403
BssAI RCCGGY 2 cut(s) 484, 1047
BssECI CCNNGG 2 cut(s) 56, 798
BssMI GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
BssT1I CCWWGG 2 cut(s) 56, 798
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 896
Bst6I CTCTTC 2 cut(s) 483, 829
BstAPI GCANNNNNTGC 1 cut(s) 340
BstAUI TGTACA 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 917
BstC8I GCNNGC 2 cut(s) 87, 160
BstDEI CTNAG 2 cut(s) 81, 875
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 4 cut(s) 559, 567, 859, 984
BstKTI GATC 7 cut(s) 14, 126, 358, 503, 541, 883, 960
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
BstMWI GCNNNNNNNGC 2 cut(s) 340, 794
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 885
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 4 cut(s) 167, 469, 1011, 1015
BstX2I RGATCY 1 cut(s) 957
BstXI CCANNNNNNTGG 2 cut(s) 410, 634
BstYI RGATCY 1 cut(s) 957
BsuRI GGCC 4 cut(s) 402, 525, 842, 1021
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 4 cut(s) 559, 567, 859, 984
Cac8I GCNNGC 2 cut(s) 87, 160
Cfr10I RCCGGY 2 cut(s) 484, 1047
Cfr13I GGNCC 1 cut(s) 840
Csp6I GTAC 7 cut(s) 53, 146, 249, 287, 328, 706, 906
CspAI ACCGGT 1 cut(s) 1047
CspCI CAANNNNNGTGG 2 cut(s) 1032, 1067
CviAII CATG 6 cut(s) 57, 103, 290, 340, 439, 632
CviQI GTAC 7 cut(s) 53, 146, 249, 287, 328, 706, 906
DdeI CTNAG 2 cut(s) 81, 875
DpnI GATC 7 cut(s) 13, 125, 357, 502, 540, 882, 959
DpnII GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
DraI TTTAAA 1 cut(s) 657
DriI GACNNNNNGTC 2 cut(s) 25, 196
EaeI YGGCCR 2 cut(s) 400, 1019
Eam1104I CTCTTC 2 cut(s) 483, 829
Eam1105I GACNNNNNGTC 2 cut(s) 25, 196
EarI CTCTTC 2 cut(s) 483, 829
Eco130I CCWWGG 2 cut(s) 56, 798
Eco32I GATATC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 840
EcoRII CCWGG 1 cut(s) 21
EcoRV GATATC 1 cut(s) 832
EcoT14I CCWWGG 2 cut(s) 56, 798
EcoT22I ATGCAT 1 cut(s) 588
ErhI CCWWGG 2 cut(s) 56, 798
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 6 cut(s) 60, 106, 293, 343, 442, 635
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 584
FatI CATG 6 cut(s) 56, 102, 289, 339, 438, 631
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 6 cut(s) 156, 315, 458, 1004, 1022, 1025
FokI GGATG 4 cut(s) 554, 566, 846, 991
Fsp4HI GCNGC 6 cut(s) 156, 315, 458, 1004, 1022, 1025
FspBI CTAG 3 cut(s) 453, 603, 866
GluI GCNGC 6 cut(s) 156, 315, 458, 1004, 1022, 1025
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 4 cut(s) 402, 525, 842, 1021
HapII CCGG 2 cut(s) 485, 1048
Hin1II CATG 6 cut(s) 60, 106, 293, 343, 442, 635
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 5 cut(s) 26, 242, 271, 926, 1009
HpaII CCGG 2 cut(s) 485, 1048
HphI GGTGA 4 cut(s) 386, 495, 1026, 1076
Hpy166II GTNNAC 4 cut(s) 255, 295, 377, 436
Hpy188I TCNGA 5 cut(s) 64, 829, 901, 931, 1077
Hpy188III TCNNGA 4 cut(s) 34, 268, 275, 847
Hpy8I GTNNAC 4 cut(s) 255, 295, 377, 436
HpyAV CCTTC 4 cut(s) 100, 853, 896, 1055
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 3 cut(s) 251, 701, 916
HpyCH4V TGCA 3 cut(s) 99, 586, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 340, 794
HpyF3I CTNAG 2 cut(s) 81, 875
HpySE526I ACGT 3 cut(s) 251, 701, 916
Hsp92II CATG 6 cut(s) 60, 106, 293, 343, 442, 635
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
LmnI GCTCC 1 cut(s) 450
Lsp1109I GCAGC 4 cut(s) 167, 469, 1011, 1015
LweI GCATC 3 cut(s) 432, 544, 573
MaeI CTAG 3 cut(s) 453, 603, 866
MaeII ACGT 3 cut(s) 251, 701, 916
MaeIII GTNAC 5 cut(s) 198, 490, 531, 672, 917
MalI GATC 7 cut(s) 13, 125, 357, 502, 540, 882, 959
MboI GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
MboII GAAGA 3 cut(s) 470, 725, 816
MflI RGATCY 1 cut(s) 957
MhlI GDGCHC 1 cut(s) 790
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 2 cut(s) 335, 392
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 2 cut(s) 35, 236
MmeI TCCRAC 1 cut(s) 531
Mox20I TGGCCA 1 cut(s) 402
Mph1103I ATGCAT 1 cut(s) 588
MscI TGGCCA 1 cut(s) 402
MseI TTAA 1 cut(s) 656
MslI CAYNNNNRTG 3 cut(s) 369, 591, 977
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 1006, 1024
MspI CCGG 2 cut(s) 485, 1048
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 340, 794
NcoI CCATGG 1 cut(s) 56
NdeII GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
NlaIII CATG 6 cut(s) 60, 106, 293, 343, 442, 635
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 4 cut(s) 198, 490, 672, 917
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 1 cut(s) 293
PaeR7I CTCGAG 1 cut(s) 34
PciI ACATGT 1 cut(s) 289
PfeI GAWTC 3 cut(s) 271, 926, 1009
PinAI ACCGGT 1 cut(s) 1047
PkrI GCNGC 6 cut(s) 157, 316, 459, 1005, 1023, 1026
PleI GAGTC 2 cut(s) 34, 236
PpsI GAGTC 2 cut(s) 34, 236
Ppu21I YACGTR 1 cut(s) 917
PscI ACATGT 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 840
PsuI RGATCY 1 cut(s) 957
RsaI GTAC 7 cut(s) 54, 147, 250, 288, 329, 707, 907
RsaNI GTAC 7 cut(s) 53, 146, 249, 287, 328, 706, 906
RseI CAYNNNNRTG 3 cut(s) 369, 591, 977
SaqAI TTAA 1 cut(s) 656
SatI GCNGC 6 cut(s) 156, 315, 458, 1004, 1022, 1025
Sau3AI GATC 7 cut(s) 11, 123, 355, 500, 538, 880, 957
Sau96I GGNCC 1 cut(s) 840
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 2 cut(s) 35, 236
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 3 cut(s) 432, 544, 573
SfcI CTRYAG 1 cut(s) 885
Sfr274I CTCGAG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 3 cut(s) 369, 591, 977
SmlI CTYRAG 2 cut(s) 34, 647
SmoI CTYRAG 2 cut(s) 34, 647
Sse9I AATT 2 cut(s) 335, 392
SsiI CCGC 4 cut(s) 314, 519, 1006, 1022
SspMI CTAG 3 cut(s) 453, 603, 866
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 2 cut(s) 56, 798
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 3 cut(s) 254, 704, 919
TaqI TCGA 1 cut(s) 35
TasI AATT 2 cut(s) 335, 392
TatI WGTACW 3 cut(s) 145, 286, 705
TauI GCSGC 2 cut(s) 317, 1024
TfiI GAWTC 3 cut(s) 271, 926, 1009
Tru1I TTAA 1 cut(s) 656
Tru9I TTAA 1 cut(s) 656
TseFI GTSAC 4 cut(s) 198, 490, 672, 917
TseI GCWGC 4 cut(s) 155, 457, 1003, 1024
Tsp45I GTSAC 4 cut(s) 198, 490, 672, 917
TspDTI ATGAA 7 cut(s) 296, 324, 328, 654, 718, 759, 1074
TspGWI ACGGA 2 cut(s) 197, 979
XceI RCATGY 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 3 cut(s) 453, 603, 866
ZrmI AGTACT 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.