Rroxscaffold_6G00418380

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002962
Physical Location & Seq
Forward (+)
39893243 .. 39900317
7075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418380.1

Sequence Viewer

Length: 1914 bp
ATGGCATTGACTTCATTAGCTTTTCCTAGCTCTCTGGCTTCATCAACAAAAGATATCCCCAAAGAGTACAATGATCTTCACCAGTCACACTCTAGCAGAGTCCTATGCAATCGTGCTAGTAGCCAGGGAAGGTTTTCTTCAGCTGATAACCAGCTTTGTTATTGCATCAACCGTATCCATGCAACATCAACAGAACCAGAACCAAAGAAGAGTCCTTTCGTGGGAATTTCCTCTTTTCAAAGTCGGGGTTCTATCATTCCAAATTTGCTACGATCATATTCATTTGATCCAGACTCGAGTAAGCATATGAACATAGTGGAGAATGACAGAAATGAGGTGGGGAGCTCTCGGGAGAGCAGTGAAGAGGAGGAACTCAAGAGAACCAGTTGGGTGGAGAGTCTTGTGGAACTCGGCAGACACAACTGGAAGAATATTTGGCAGCAAAAAGTGGTTGGGGGTGAAGACAACGACTGTGATGAGGAAAGGAAACGCAGATCATATGATCGTGACCTTTTCTCGAGATTTTTGGTGGAAGTACCATGGTCAGATACCAAATTATTCTCTCAGCTAGCCCTCTTGTGCAACTTGGCTTATGTGATACCAGAGATCAGGGACACGGATTTAAAGACATACTATGGCCTACAATTTGTAACATCTTTATTAGATAAGCAAGCAAAGGCAGATGGAATTGTGAGTATCAAACCAAAACTTGATCAGGACTCTACTGCAGTGAATTCTAAGGCCTCAGCTGCAGCTTATCTCCGCTCAGTAAGCAAATCACAGCAGGAGGTTGCTGGAGAGAATTCATGCAGAAATGGAGGGAGCTCACCTCAAAATCATAACTCAGAGGTAGATGCTTCCCTCACAGCATCAACAATGACGTTGATGCTTGCAGCAGGTAATGAGGAAAAGCAAGGAGCAGCAAAGGATATTCAGTCACTGCACTCCTTACCTTGTGAGTGGTTTGTTTGTGATGATCCCACTACATATACTCGTTTCTTTGTGATTCAGGGAACTAGTGTACTTGTTCACCGAGGAATTTATGAACTGGCAAAGGAAATTTACCAGCAGTTCAAACCCAAAATAATTGACCACTTGGCAAGATATGGGGAGCAAGCGAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTCTCTGTTATACTCCACTTGATGCTACTGACTACGAAGCTCGTCAAACCCTCAACTCTTCTGCCTGTTGTCACATTTGGCTCCCCATATGTGTTCAGTGGAGGCCAGAAAATATTTGAGCAACTAGGATTGGATGAGTATGATCACATCCGTTGTGTTATGCTGCACAGAGATATTGTCCCTAGATCTTTCTCCTCTAACTACCCTAATCATGTCGCAGCAATTCTTAAGCATTTACATGGTTCATTTGAGTCACATCCTTGTCTAACAAAGAATGTATGTATCAATCTATACAACCAAATTCATAAAATCCTCCATAAATTTATGCAGTTATGTGCGGACTTAAACTCATATGAATTATGTGTTTCTATGCAGAAGATATTGTACTCTCCTATGGGTAAACTATTTATTGTCCAACCTGATGAAAAGTCATCTCCTCCACACCCTCTACTCCCTTCAGGGAGCTCTCTCTATGCTTTGGACAGTACCCAATGTGGATTCTCCTCGAGCGTCTTGAGGGACTTTTTGAATTCCCCACATCCACTAGAAACACTAAGTGATCCGACAGCGTATGGTTCGGAAGGTACAATTCTAAGGGACCATGACTCGAGCAACTATCTAAAGGCTGTGAATGGGGTCCTGAGGCTATACCAAAGGAAGGTCGTCAAGAAAGCTAGGAAGCAGAGGAATATCTTGTGGCCGCTGCTTAATTTACCATCTCCAAAATCATTGAACCCTGAAGATGAAAGTAACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

637

Amino Acids

71.39

Weight (kDa)

7.3

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 337 - 450 3.2e-19 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 765
AciI CCGC 3 cut(s) 763, 1493, 1856
AclI AACGTT 1 cut(s) 1908
AclWI GGATC 3 cut(s) 281, 971, 1709
AcoI YGGCCR 2 cut(s) 1135, 1853
AcsI RAATTY 9 cut(s) 225, 262, 733, 802, 1038, 1059, 1455, 1475, 1684
AcuI CTGAAG 3 cut(s) 123, 1596, 1914
AdeI CACNNNGTG 1 cut(s) 1712
AfaI GTAC 6 cut(s) 68, 537, 1023, 1541, 1642, 1741
AfiI CCNNNNNNNGG 2 cut(s) 221, 1813
AflII CTTAAG 1 cut(s) 1382
AgsI TTSAA 5 cut(s) 239, 1075, 1127, 1684, 1888
AhlI ACTAGT 1 cut(s) 1016
AjnI CCWGG 1 cut(s) 123
Alw21I GWGCWC 3 cut(s) 347, 827, 1622
AlwI GGATC 3 cut(s) 281, 971, 1709
AlwNI CAGNNNCTG 1 cut(s) 940
Ama87I CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
AoxI GGCC 5 cut(s) 637, 741, 1135, 1258, 1853
ApeKI GCWGC 8 cut(s) 439, 749, 752, 893, 920, 1318, 1373, 1858
ApoI RAATTY 9 cut(s) 225, 262, 733, 802, 1038, 1059, 1455, 1475, 1684
Asp700I GAANNNNTTC 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 1753, 1792
AsuHPI GGTGA 4 cut(s) 71, 470, 819, 1022
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
AvaII GGWCC 2 cut(s) 1753, 1792
AxyI CCTNAGG 1 cut(s) 1796
BalI TGGCCA 1 cut(s) 1137
BanII GRGCYC 3 cut(s) 347, 827, 1622
BarI GAAGNNNNNNTAC 2 cut(s) 1523, 1555
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 3 cut(s) 347, 827, 1622
BbvCI CCTCAGC 1 cut(s) 745
BbvI GCAGC 8 cut(s) 451, 736, 764, 905, 932, 1305, 1385, 1845
BccI CCATC 2 cut(s) 677, 1879
BciT130I CCWGG 1 cut(s) 125
BciVI GTATCC 1 cut(s) 185
BclI TGATCA 2 cut(s) 712, 1297
BcuI ACTAGT 1 cut(s) 1016
BfaI CTAG 9 cut(s) 27, 93, 117, 569, 1017, 1280, 1338, 1700, 1830
BfmI CTRYAG 2 cut(s) 726, 750
BfrI CTTAAG 1 cut(s) 1382
BfuAI ACCTGC 1 cut(s) 887
BfuI GTATCC 1 cut(s) 185
BglII AGATCT 1 cut(s) 1340
BisI GCNGC 9 cut(s) 440, 750, 753, 894, 921, 1319, 1374, 1856, 1859
BlsI GCNGC 9 cut(s) 441, 751, 754, 895, 922, 1320, 1375, 1857, 1860
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 1753, 1792
BmeT110I CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
BmgT120I GGNCC 2 cut(s) 1753, 1792
BmiI GGNNCC 3 cut(s) 1237, 1754, 1793
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 5 cut(s) 174, 844, 876, 878, 1167
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 468
BplI GAGNNNNNCTC 4 cut(s) 814, 846, 1140, 1172
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 359, 1690
BsaJI CCNNGG 3 cut(s) 124, 539, 1033
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 1813
Bse1I ACTGG 5 cut(s) 82, 384, 428, 1053, 1138
Bse21I CCTNAGG 1 cut(s) 1796
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 3 cut(s) 124, 539, 1033
BseGI GGATG 4 cut(s) 1294, 1302, 1411, 1693
BseLI CCNNNNNNNGG 2 cut(s) 221, 1813
BseMII CTCAG 5 cut(s) 578, 759, 780, 858, 1787
BseNI ACTGG 5 cut(s) 82, 384, 428, 1053, 1138
BseRI GAGGAG 4 cut(s) 380, 1339, 1581, 1648
BseXI GCAGC 8 cut(s) 451, 736, 764, 905, 932, 1305, 1385, 1845
BsgI GTGCAG 2 cut(s) 926, 1304
BshFI GGCC 5 cut(s) 639, 743, 1137, 1260, 1855
BsiHKAI GWGCWC 3 cut(s) 347, 827, 1622
BsiHKCI CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
BslFI GGGAC 4 cut(s) 626, 1319, 1688, 1766
BslI CCNNNNNNNGG 2 cut(s) 221, 1813
BsmFI GGGAC 4 cut(s) 626, 1319, 1688, 1766
BsnI GGCC 5 cut(s) 639, 743, 1137, 1260, 1855
BsoBI CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
Bsp1286I GDGCHC 3 cut(s) 347, 827, 1622
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 3 cut(s) 763, 1493, 1856
BspANI GGCC 5 cut(s) 639, 743, 1137, 1260, 1855
BspCNI CTCAG 5 cut(s) 577, 758, 779, 857, 1788
BspLI GGNNCC 3 cut(s) 1237, 1754, 1793
BspMAI CTGCAG 2 cut(s) 730, 754
BspMI ACCTGC 1 cut(s) 887
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 3 cut(s) 281, 971, 1709
BspTI CTTAAG 1 cut(s) 1382
BsrBI CCGCTC 1 cut(s) 765
BsrI ACTGG 5 cut(s) 82, 384, 428, 1053, 1138
BssECI CCNNGG 3 cut(s) 124, 539, 1033
BssT1I CCWWGG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 173, 473, 1640
Bst6I CTCTTC 3 cut(s) 203, 357, 1218
BstAFI CTTAAG 1 cut(s) 1382
BstC8I GCNNGC 4 cut(s) 570, 672, 891, 1116
BstDEI CTNAG 8 cut(s) 564, 738, 745, 766, 844, 1709, 1748, 1796
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 4 cut(s) 1294, 1302, 1411, 1693
BstMWI GCNNNNNNNGC 3 cut(s) 749, 771, 1120
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 2 cut(s) 726, 750
BstV1I GCAGC 8 cut(s) 451, 736, 764, 905, 932, 1305, 1385, 1845
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 1340
BstXI CCANNNNNNTGG 2 cut(s) 391, 1145
BstYI RGATCY 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 1796
BsuI GTATCC 1 cut(s) 185
BsuRI GGCC 5 cut(s) 639, 743, 1137, 1260, 1855
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 4 cut(s) 1294, 1302, 1411, 1693
BtsI GCAGTG 3 cut(s) 364, 735, 938
BtsIMutI CAGTG 4 cut(s) 364, 735, 938, 1258
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 4 cut(s) 570, 672, 891, 1116
CaiI CAGNNNCTG 1 cut(s) 940
Cfr13I GGNCC 2 cut(s) 1753, 1792
CseI GACGC 1 cut(s) 1654
Csp6I GTAC 6 cut(s) 67, 536, 1022, 1540, 1641, 1740
CviAII CATG 6 cut(s) 179, 540, 807, 1367, 1394, 1757
CviQI GTAC 6 cut(s) 67, 536, 1022, 1540, 1641, 1740
DdeI CTNAG 8 cut(s) 564, 738, 745, 766, 844, 1709, 1748, 1796
DraI TTTAAA 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 1712
EaeI YGGCCR 2 cut(s) 1135, 1853
Eam1104I CTCTTC 3 cut(s) 203, 357, 1218
EarI CTCTTC 3 cut(s) 203, 357, 1218
Ecl136II GAGCTC 3 cut(s) 345, 825, 1620
Eco130I CCWWGG 1 cut(s) 539
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 3 cut(s) 347, 827, 1622
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 1753, 1792
Eco53kI GAGCTC 3 cut(s) 345, 825, 1620
Eco57I CTGAAG 3 cut(s) 123, 1596, 1914
Eco81I CCTNAGG 1 cut(s) 1796
Eco88I CYCGRG 5 cut(s) 295, 348, 517, 1660, 1762
EcoICRI GAGCTC 3 cut(s) 345, 825, 1620
EcoO109I RGGNCCY 1 cut(s) 1792
EcoRI GAATTC 3 cut(s) 733, 802, 1684
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 539
EcoT38I GRGCYC 3 cut(s) 347, 827, 1622
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 6 cut(s) 182, 543, 810, 1370, 1397, 1760
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 1107, 1139
FaqI GGGAC 4 cut(s) 626, 1319, 1688, 1766
FatI CATG 6 cut(s) 178, 539, 806, 1366, 1393, 1756
FauNDI CATATG 4 cut(s) 306, 499, 1243, 1507
FbaI TGATCA 2 cut(s) 712, 1297
Fnu4HI GCNGC 9 cut(s) 440, 750, 753, 894, 921, 1319, 1374, 1856, 1859
FokI GGATG 4 cut(s) 1289, 1301, 1398, 1680
FriOI GRGCYC 3 cut(s) 347, 827, 1622
Fsp4HI GCNGC 9 cut(s) 440, 750, 753, 894, 921, 1319, 1374, 1856, 1859
FspBI CTAG 9 cut(s) 27, 93, 117, 569, 1017, 1280, 1338, 1700, 1830
GluI GCNGC 9 cut(s) 440, 750, 753, 894, 921, 1319, 1374, 1856, 1859
GsuI CTGGAG 1 cut(s) 816
HaeIII GGCC 5 cut(s) 639, 743, 1137, 1260, 1855
HgaI GACGC 1 cut(s) 1654
Hin1II CATG 6 cut(s) 182, 543, 810, 1370, 1397, 1760
HindIII AAGCTT 1 cut(s) 1121
HinfI GANTC 9 cut(s) 99, 211, 293, 397, 719, 1006, 1406, 1653, 1760
HphI GGTGA 4 cut(s) 71, 470, 819, 1022
Hpy166II GTNNAC 3 cut(s) 1022, 1030, 1556
Hpy188I TCNGA 4 cut(s) 547, 847, 1719, 1735
Hpy8I GTNNAC 3 cut(s) 1022, 1030, 1556
HpyAV CCTTC 4 cut(s) 123, 1620, 1730, 1807
HpyCH4III ACNGT 3 cut(s) 173, 473, 1640
HpyCH4IV ACGT 2 cut(s) 881, 1908
HpyF10VI GCNNNNNNNGC 3 cut(s) 749, 771, 1120
HpyF3I CTNAG 8 cut(s) 564, 738, 745, 766, 844, 1709, 1748, 1796
HpySE526I ACGT 2 cut(s) 881, 1908
Hsp92II CATG 6 cut(s) 182, 543, 810, 1370, 1397, 1760
Ksp22I TGATCA 2 cut(s) 712, 1297
LmnI GCTCC 6 cut(s) 342, 822, 917, 1111, 1241, 1617
Lsp1109I GCAGC 8 cut(s) 451, 736, 764, 905, 932, 1305, 1385, 1845
LweI GCATC 5 cut(s) 174, 844, 876, 878, 1167
MaeI CTAG 9 cut(s) 27, 93, 117, 569, 1017, 1280, 1338, 1700, 1830
MaeII ACGT 2 cut(s) 881, 1908
MaeIII GTNAC 7 cut(s) 84, 506, 649, 936, 1225, 1407, 1904
MbiI CCGCTC 1 cut(s) 765
MboII GAAGA 9 cut(s) 68, 129, 220, 374, 439, 473, 1205, 1543, 1907
MflI RGATCY 1 cut(s) 1340
MhlI GDGCHC 3 cut(s) 347, 827, 1622
MlsI TGGCCA 1 cut(s) 1137
MluNI TGGCCA 1 cut(s) 1137
MlyI GAGTC 7 cut(s) 108, 220, 287, 406, 713, 1415, 1754
MmeI TCCRAC 2 cut(s) 1594, 1742
Mox20I TGGCCA 1 cut(s) 1137
MroXI GAANNNNTTC 1 cut(s) 133
MscI TGGCCA 1 cut(s) 1137
MseI TTAA 4 cut(s) 623, 1383, 1499, 1863
MslI CAYNNNNRTG 1 cut(s) 1392
Msp20I TGGCCA 1 cut(s) 1137
MspA1I CMGCKG 3 cut(s) 143, 749, 1858
MspCI CTTAAG 1 cut(s) 1382
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 749, 771, 1120
NcoI CCATGG 1 cut(s) 539
NdeI CATATG 4 cut(s) 306, 499, 1243, 1507
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 6 cut(s) 182, 543, 810, 1370, 1397, 1760
NlaIV GGNNCC 3 cut(s) 1237, 1754, 1793
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 5 cut(s) 84, 506, 936, 1225, 1407
PaeR7I CTCGAG 4 cut(s) 295, 517, 1660, 1762
PceI AGGCCT 1 cut(s) 743
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 1006, 1653
PkrI GCNGC 9 cut(s) 441, 751, 754, 895, 922, 1320, 1375, 1857, 1860
PleI GAGTC 7 cut(s) 107, 219, 287, 405, 713, 1414, 1754
PpsI GAGTC 7 cut(s) 107, 219, 287, 405, 713, 1414, 1754
PpuMI RGGWCCY 1 cut(s) 1792
Psp124BI GAGCTC 3 cut(s) 347, 827, 1622
Psp1406I AACGTT 1 cut(s) 1908
Psp5II RGGWCCY 1 cut(s) 1792
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 1237, 1754, 1793
PspPI GGNCC 2 cut(s) 1753, 1792
PspPPI RGGWCCY 1 cut(s) 1792
PspXI VCTCGAGB 3 cut(s) 295, 1660, 1762
PstI CTGCAG 2 cut(s) 730, 754
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 1340
PvuII CAGCTG 2 cut(s) 143, 749
RsaI GTAC 6 cut(s) 68, 537, 1023, 1541, 1642, 1741
RsaNI GTAC 6 cut(s) 67, 536, 1022, 1540, 1641, 1740
RseI CAYNNNNRTG 1 cut(s) 1392
SacI GAGCTC 3 cut(s) 347, 827, 1622
SaqAI TTAA 4 cut(s) 623, 1383, 1499, 1863
SatI GCNGC 9 cut(s) 440, 750, 753, 894, 921, 1319, 1374, 1856, 1859
Sau96I GGNCC 2 cut(s) 1753, 1792
SchI GAGTC 7 cut(s) 108, 220, 287, 406, 713, 1415, 1754
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 3 cut(s) 347, 827, 1622
SfaNI GCATC 5 cut(s) 174, 844, 876, 878, 1167
SfcI CTRYAG 2 cut(s) 726, 750
Sfr274I CTCGAG 4 cut(s) 295, 517, 1660, 1762
SinI GGWCC 2 cut(s) 1753, 1792
SlaI CTCGAG 4 cut(s) 295, 517, 1660, 1762
SmiMI CAYNNNNRTG 1 cut(s) 1392
SmlI CTYRAG 7 cut(s) 295, 374, 517, 1382, 1660, 1669, 1762
SmoI CTYRAG 7 cut(s) 295, 374, 517, 1382, 1660, 1669, 1762
SpeI ACTAGT 1 cut(s) 1016
SseBI AGGCCT 1 cut(s) 743
SsiI CCGC 3 cut(s) 763, 1493, 1856
SspI AATATT 2 cut(s) 433, 1269
SspMI CTAG 9 cut(s) 27, 93, 117, 569, 1017, 1280, 1338, 1700, 1830
SstI GAGCTC 3 cut(s) 347, 827, 1622
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 3 cut(s) 173, 473, 1640
TaiI ACGT 2 cut(s) 884, 1911
TaqI TCGA 4 cut(s) 296, 518, 1661, 1763
TatI WGTACW 3 cut(s) 66, 1021, 1539
TauI GCSGC 1 cut(s) 1858
TfiI GAWTC 2 cut(s) 1006, 1653
Tru1I TTAA 4 cut(s) 623, 1383, 1499, 1863
Tru9I TTAA 4 cut(s) 623, 1383, 1499, 1863
TscAI CASTG 4 cut(s) 364, 735, 945, 1258
TseFI GTSAC 5 cut(s) 84, 506, 936, 1225, 1407
TseI GCWGC 8 cut(s) 439, 749, 752, 893, 920, 1318, 1373, 1858
Tsp45I GTSAC 5 cut(s) 84, 506, 936, 1225, 1407
TspGWI ACGGA 2 cut(s) 632, 1295
TspRI CASTG 4 cut(s) 364, 735, 945, 1258
Vha464I CTTAAG 1 cut(s) 1382
VpaK11BI GGWCC 2 cut(s) 1753, 1792
XapI RAATTY 9 cut(s) 225, 262, 733, 802, 1038, 1059, 1455, 1475, 1684
XhoI CTCGAG 4 cut(s) 295, 517, 1660, 1762
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 9 cut(s) 27, 93, 117, 569, 1017, 1280, 1338, 1700, 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.