Rh1CG266900

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002962
Physical Location & Seq
Forward (+)
53224193 .. 53230754
6562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG266900.1

Sequence Viewer

Length: 2055 bp
ATGGCCTGCACTTCATTATCTATACCTAGCTCTCCGGCTTCAACGAGGACTATTGACGTCTTCAAGGAGCAGAGTGGTCTTCGGCGATCGCAATCCAGCAAAGACCTTTACAGACGTGCAAATATTCGGAGGTCTTATTCCGATAACCATCTTTGCTACCGCATTAGGCCTATTCATGCTGCGTCAGCCCAACCAAAACTAAAGAACAGCCGGTCCATGGGGTTTTTCCCCTTTCAATTTTCGAGCTCCATTCTACCAAACTTGGTTAGATCATTTTTGTTTGATGCAGACACGAGTAAGAATATGAGCACGGCAGAGAGGGAAATGGAAATGATCGATAACTCTAGGGAGAGCGGTGAAGAAGAAGAAGATCATGAGATAAAGAGAGCCAATTGGGTGGAGAGGCTTGTGGAGATCAGAAGAAAATGGAGTAATAGGCAGCAAAAAGAAGTTGGGGATGAAGACAGTGTTTGTCACGAAGGTGATGGTGAATGTGAATGTGGAGAGGGTGGTTGTTTAGTGGATTACAGTACAGATGAAGATGATGGAGAGATAACTTCAGAAAGTTTCCAAAGGTTATTGGTCAAGGTACCATGGTCTGATACCAAACAATTTTCTCAGCTAGCCTTCTTGAGCAACCTGGCATATGTAATACCATTGATAAAGGCCGATGATTTGAAGAGATATTACGGCCTACAGTTTGTAACATCTTCATTAATAAAGAAAGCAGAGGAAGAGGAACTCAAAGAAGAAATTGATCAGGACTCTACTCGTGTACCTGTAACTGACGAGGAATCCACTTCAGAGGCAGCTGCAGACTCTGAGCAGAAGCATTCAATCCATCCATCTGTTGCTTACGAAATTGCTGCCTCAGCAGCATCTTATGTTCGCTCGCGTGACATGGATGGCGGTGATGTAGAGAATTCATTCAGAAGTGGAGACCAACAACAACAACGAGAGGAAGGAGGGAGCTCCCCCCGAAATTATAAGTCGGAGGTAGCTGTTTCCTTAACAGCATCAACGATGACAGTCGTGGTTGCAGCAGGGGAAAAACAAAAGCAAGAAACAGCACAAGACCTTCGGTCACCCCAATCTGCACCTTGTGAATGGTTTGTTTGTGATGACAACAGTACATATACTCGCTGCTTTGTGATTCAGGGTTCGGACTCCCTTGCGTCCTGGCAGGCAAACCTCTTCTTTGAACCTACAAAATTTGAGGGAACAAATGTACTTGTTCACAGAGGAATCTATGAAGCAGCAAAGGGGATGTACGAGCAATTCATGCCACAGATAATCGAGCACTTGGATAGATATGGAGAGCGTGCAAAGTTTCAGTTCACTGGTCATTCTCTTGGAGGAAGCCTCTCTCTTCTAGTCCACTTGATGTTACTGACCAGGAACTTTGTCAAGCCCTCCACTCTTCGGCCAGTTGTGACATTTGGCTCACCATTTGTGTTTTGTGGAGGCCAGAAAATACTAGACGACCTGGGTGTCGATGAGAACCAAATCCATTGCGTGATGATGCATAGAGATATTGTCCCAAGAGCCTTCTCCTGCAACTACCCTAACCATGTGGCTACGCTTCTCAAGCGTTTAAATGGTTCATTTCGGTCACACCCTTGTCTGATAAAAAACAAAATATTGTACTCTCCTATGGGTAAAATATTCATTCTGCAACCTGATGAAAGCTTGTCTCCGCCACACCCTCTTCTCCCCTTGGGGAGTGCCCTCTATACTTTGGACAAAACTCAACATGGGTACTCTTCAAGCGTCCTTAGAACCTTCTTAAATTCCCCGCATCCACTAGAAACCCTAAGCGATCCTACAGCTTATGGTTCGGAAGGTACAATCCTACGGGATCATGACTCGAGCAACTATCTGAAGGCTGTGAATGGTGTCCTTAGGCTACACACAAGAAAGGTTGTTCGTATAGCTAGGAAACAGAGGAATGTCTTGTGGCCGCTACTTACTTCACCATCCCCACACACATGGAACCATGAAAATAATCTGGAGAGCGGCAGATTGGTGACGAAGGAGATTCTCACAGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

684

Amino Acids

77.01

Weight (kDa)

5.94

Isoelectric Point (pI)

58.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 383 - 520 1.3e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
AasI GACNNNNNNGTC 1 cut(s) 1490
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 2 cut(s) 354, 2016
AccII CGCG 1 cut(s) 895
AciI CCGC 7 cut(s) 160, 354, 909, 1697, 1796, 1961, 2016
AclWI GGATC 2 cut(s) 1814, 1866
AcoI YGGCCR 2 cut(s) 1424, 1958
AcsI RAATTY 3 cut(s) 922, 1211, 1789
AcuI CTGAAG 3 cut(s) 543, 786, 1901
AcyI GRCGYC 1 cut(s) 57
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 9 cut(s) 532, 591, 777, 1132, 1230, 1271, 1646, 1760, 1846
AfiI CCNNNNNNNGG 2 cut(s) 217, 1422
AgsI TTSAA 7 cut(s) 42, 64, 236, 679, 837, 1202, 1767
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 116
AjnI CCWGG 4 cut(s) 639, 1178, 1394, 1485
AleI CACNNNNGTG 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 9 cut(s) 30, 246, 622, 812, 972, 1001, 1689, 1829, 1934
AluI AGCT 9 cut(s) 30, 246, 622, 812, 972, 1001, 1689, 1829, 1934
Alw21I GWGCWC 4 cut(s) 248, 311, 974, 1302
Alw26I GTCTC 2 cut(s) 933, 1698
AlwI GGATC 2 cut(s) 1814, 1866
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 1 cut(s) 1867
AoxI GGCC 7 cut(s) 3, 167, 666, 691, 1424, 1465, 1958
ApeKI GCWGC 9 cut(s) 179, 439, 809, 812, 866, 875, 1040, 1143, 1256
ApoI RAATTY 3 cut(s) 922, 1211, 1789
AseI ATTAAT 1 cut(s) 716
AsiSI GCGATCGC 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 926
Asp718I GGTACC 1 cut(s) 589
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 8 cut(s) 368, 494, 500, 923, 1077, 1437, 1965, 2038
AsuNHI GCTAGC 1 cut(s) 622
AvaI CYCGRG 1 cut(s) 1867
AvaII GGWCC 1 cut(s) 213
AxyI CCTNAGG 1 cut(s) 1901
BaeGI GKGCMC 1 cut(s) 1729
BaeI ACNNNNGTAYC 4 cut(s) 581, 614, 759, 792
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 248, 974
BarI GAAGNNNNNNTAC 2 cut(s) 671, 703
BauI CACGAG 2 cut(s) 292, 771
BbsI GAAGAC 3 cut(s) 52, 71, 468
Bbv12I GWGCWC 4 cut(s) 248, 311, 974, 1302
BbvCI CCTCAGC 1 cut(s) 871
BbvI GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BccI CCATC 7 cut(s) 156, 479, 539, 849, 853, 899, 1984
BceAI ACGGC 2 cut(s) 327, 706
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 4 cut(s) 641, 1180, 1396, 1487
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 2 cut(s) 933, 1698
BfaI CTAG 7 cut(s) 27, 345, 623, 1373, 1478, 1805, 1935
BfmI CTRYAG 3 cut(s) 695, 813, 1824
Bme1390I CCNGG 4 cut(s) 641, 1180, 1396, 1487
Bme18I GGWCC 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmeT110I CYCGRG 1 cut(s) 1867
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 591, 1994
BmrFI CCNGG 4 cut(s) 641, 1180, 1396, 1487
BmsI GCATC 5 cut(s) 274, 887, 1025, 1512, 1807
BmtI GCTAGC 1 cut(s) 626
BpiI GAAGAC 3 cut(s) 52, 71, 468
BplI GAGNNNNNCTC 2 cut(s) 1347, 1379
BpmI CTGGAG 1 cut(s) 2030
Bpu10I CCTNAGC 2 cut(s) 871, 1814
BpuEI CTTGAG 2 cut(s) 652, 1571
Bsa29I ATCGAT 1 cut(s) 336
BsaBI GATNNNNATC 2 cut(s) 91, 147
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 933
BsaJI CCNNGG 4 cut(s) 216, 593, 1486, 1716
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 1422
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 1345, 1427
Bse21I CCTNAGG 1 cut(s) 1901
Bse3DI GCAATG 1 cut(s) 1510
Bse8I GATNNNNATC 2 cut(s) 91, 147
BseBI CCWGG 4 cut(s) 641, 1180, 1396, 1487
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 216, 593, 1486, 1716
BseGI GGATG 6 cut(s) 463, 841, 910, 1272, 1798, 1976
BseJI GATNNNNATC 2 cut(s) 91, 147
BseLI CCNNNNNNNGG 2 cut(s) 217, 1422
BseMI GCAATG 1 cut(s) 1510
BseMII CTCAG 3 cut(s) 632, 813, 885
BseNI ACTGG 2 cut(s) 1345, 1427
BseSI GKGCMC 1 cut(s) 1729
BseXI GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 895
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 7 cut(s) 5, 169, 668, 693, 1426, 1467, 1960
BshNI GGYRCC 1 cut(s) 589
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 248, 311, 974, 1302
BsiHKCI CYCGRG 1 cut(s) 1867
BsiSI CCGG 2 cut(s) 35, 211
BslFI GGGAC 1 cut(s) 1523
BslI CCNNNNNNNGG 2 cut(s) 217, 1422
BsmAI GTCTC 2 cut(s) 933, 1698
BsmFI GGGAC 1 cut(s) 1523
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 7 cut(s) 5, 169, 668, 693, 1426, 1467, 1960
Bso31I GGTCTC 1 cut(s) 933
BsoBI CYCGRG 1 cut(s) 1867
Bsp1286I GDGCHC 5 cut(s) 248, 311, 974, 1302, 1729
Bsp143I GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
Bsp19I CCATGG 2 cut(s) 216, 593
BspACI CCGC 7 cut(s) 160, 354, 909, 1697, 1796, 1961, 2016
BspANI GGCC 7 cut(s) 5, 169, 668, 693, 1426, 1467, 1960
BspCNI CTCAG 3 cut(s) 631, 814, 884
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 895
BspHI TCATGA 2 cut(s) 373, 1861
BspLI GGNNCC 2 cut(s) 591, 1994
BspMAI CTGCAG 1 cut(s) 817
BspOI GCTAGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 1814, 1866
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 933
BsrBI CCGCTC 2 cut(s) 354, 2016
BsrDI GCAATG 1 cut(s) 1510
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 1345, 1427
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 216, 593, 1486, 1716
BssMI GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 2 cut(s) 292, 771
BssT1I CCWWGG 3 cut(s) 216, 593, 1716
Bst2BI CACGAG 2 cut(s) 292, 771
Bst2UI CCWGG 4 cut(s) 641, 1180, 1396, 1487
Bst4CI ACNGT 5 cut(s) 467, 530, 699, 1030, 1130
Bst6I CTCTTC 7 cut(s) 674, 729, 1199, 1374, 1425, 1713, 1768
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstC8I GCNNGC 5 cut(s) 7, 624, 893, 1185, 1323
BstDEI CTNAG 6 cut(s) 618, 822, 871, 1775, 1814, 1901
BstDSI CCRYGG 2 cut(s) 216, 593
BstEII GGTNACC 1 cut(s) 1083
BstF5I GGATG 6 cut(s) 463, 841, 910, 1272, 1798, 1976
BstFNI CGCG 1 cut(s) 895
BstKTI GATC 8 cut(s) 89, 272, 336, 373, 417, 760, 1822, 1861
BstMAI GTCTC 2 cut(s) 933, 1698
BstMBI GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
BstNI CCWGG 4 cut(s) 641, 1180, 1396, 1487
BstPI GGTNACC 1 cut(s) 1083
BstSCI CCNGG 4 cut(s) 639, 1178, 1394, 1485
BstSFI CTRYAG 3 cut(s) 695, 813, 1824
BstSLI GKGCMC 1 cut(s) 1729
BstUI CGCG 1 cut(s) 895
BstV1I GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BstV2I GAAGAC 3 cut(s) 52, 71, 468
BstXI CCANNNNNNTGG 2 cut(s) 397, 1989
Bsu15I ATCGAT 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 1901
BsuRI GGCC 7 cut(s) 5, 169, 668, 693, 1426, 1467, 1960
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 216, 593
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 6 cut(s) 463, 841, 910, 1272, 1798, 1976
BtsIMutI CAGTG 2 cut(s) 472, 1338
Cac8I GCNNGC 5 cut(s) 7, 624, 893, 1185, 1323
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 2 cut(s) 373, 1861
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 3 cut(s) 171, 1164, 1759
Csp6I GTAC 9 cut(s) 531, 590, 776, 1131, 1229, 1270, 1645, 1759, 1845
CviQI GTAC 9 cut(s) 531, 590, 776, 1131, 1229, 1270, 1645, 1759, 1845
DdeI CTNAG 6 cut(s) 618, 822, 871, 1775, 1814, 1901
DpnI GATC 8 cut(s) 88, 271, 335, 372, 416, 759, 1821, 1860
DpnII GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
DraI TTTAAA 1 cut(s) 1596
DraIII CACNNNGTG 1 cut(s) 1103
DrdI GACNNNNNNGTC 1 cut(s) 1490
DriI GACNNNNNGTC 1 cut(s) 1081
DseDI GACNNNNNNGTC 1 cut(s) 1490
EaeI YGGCCR 2 cut(s) 1424, 1958
Eam1104I CTCTTC 7 cut(s) 674, 729, 1199, 1374, 1425, 1713, 1768
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 7 cut(s) 674, 729, 1199, 1374, 1425, 1713, 1768
EciI GGCGGA 1 cut(s) 1686
Ecl136II GAGCTC 2 cut(s) 246, 972
Eco130I CCWWGG 3 cut(s) 216, 593, 1716
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 248, 974
Eco31I GGTCTC 1 cut(s) 933
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 246, 972
Eco57I CTGAAG 3 cut(s) 543, 786, 1901
Eco81I CCTNAGG 1 cut(s) 1901
Eco88I CYCGRG 1 cut(s) 1867
Eco91I GGTNACC 1 cut(s) 1083
EcoICRI GAGCTC 2 cut(s) 246, 972
EcoO65I GGTNACC 1 cut(s) 1083
EcoRI GAATTC 1 cut(s) 922
EcoRII CCWGG 4 cut(s) 639, 1178, 1394, 1485
EcoT14I CCWWGG 3 cut(s) 216, 593, 1716
EcoT22I ATGCAT 1 cut(s) 1527
EcoT38I GRGCYC 2 cut(s) 248, 974
ErhI CCWWGG 3 cut(s) 216, 593, 1716
FaqI GGGAC 1 cut(s) 1523
FauI CCCGC 1 cut(s) 1803
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 757
FokI GGATG 6 cut(s) 470, 828, 917, 1279, 1785, 1963
FriOI GRGCYC 2 cut(s) 248, 974
FspBI CTAG 7 cut(s) 27, 345, 623, 1373, 1478, 1805, 1935
GsuI CTGGAG 1 cut(s) 2030
HaeIII GGCC 7 cut(s) 5, 169, 668, 693, 1426, 1467, 1960
HapII CCGG 2 cut(s) 35, 211
HgaI GACGC 3 cut(s) 171, 1164, 1759
Hin1I GRCGYC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 1687
HinfI GANTC 8 cut(s) 764, 794, 818, 1153, 1166, 1245, 1865, 2038
HpaII CCGG 2 cut(s) 35, 211
HphI GGTGA 8 cut(s) 368, 494, 500, 923, 1077, 1437, 1965, 2038
Hpy166II GTNNAC 4 cut(s) 776, 1237, 1338, 1378
Hpy188III TCNNGA 6 cut(s) 374, 476, 631, 761, 1862, 2009
Hpy8I GTNNAC 4 cut(s) 776, 1237, 1338, 1378
HpyAV CCTTC 9 cut(s) 473, 637, 956, 1088, 1558, 1792, 1835, 1876, 2026
HpyCH4III ACNGT 5 cut(s) 467, 530, 699, 1030, 1130
HpyCH4IV ACGT 2 cut(s) 57, 115
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
HpyF3I CTNAG 6 cut(s) 618, 822, 871, 1775, 1814, 1901
HpySE526I ACGT 2 cut(s) 57, 115
Hsp92I GRCGYC 1 cut(s) 57
KpnI GGTACC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
LmnI GCTCC 4 cut(s) 67, 251, 969, 977
Lsp1109I GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
LweI GCATC 5 cut(s) 274, 887, 1025, 1512, 1807
MaeI CTAG 7 cut(s) 27, 345, 623, 1373, 1478, 1805, 1935
MaeII ACGT 2 cut(s) 57, 115
MaeIII GTNAC 9 cut(s) 473, 703, 781, 896, 1083, 1386, 1432, 1611, 2026
MalI GATC 8 cut(s) 88, 271, 335, 372, 416, 759, 1821, 1860
MbiI CCGCTC 2 cut(s) 354, 2016
MboI GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
MfeI CAATTG 1 cut(s) 391
MhlI GDGCHC 5 cut(s) 248, 311, 974, 1302, 1729
MlyI GAGTC 4 cut(s) 758, 812, 1160, 1859
MmeI TCCRAC 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 1527
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 5 cut(s) 716, 1010, 1595, 1787, 2053
MslI CAYNNNNRTG 2 cut(s) 480, 1987
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 2 cut(s) 35, 211
MspR9I CCNGG 4 cut(s) 641, 1180, 1396, 1487
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 4 cut(s) 641, 1180, 1396, 1487
MvnI CGCG 1 cut(s) 895
MwoI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
NcoI CCATGG 2 cut(s) 216, 593
NdeI CATATG 1 cut(s) 646
NdeII GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
NheI GCTAGC 1 cut(s) 622
NlaIV GGNNCC 2 cut(s) 591, 1994
NmuCI GTSAC 6 cut(s) 473, 896, 1083, 1432, 1611, 2026
NsiI ATGCAT 1 cut(s) 1527
OliI CACNNNNGTG 1 cut(s) 480
PaeR7I CTCGAG 1 cut(s) 1867
PagI TCATGA 2 cut(s) 373, 1861
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 1 cut(s) 832
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 4 cut(s) 794, 1153, 1245, 2038
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 4 cut(s) 758, 812, 1160, 1859
PpsI GAGTC 4 cut(s) 758, 812, 1160, 1859
PshBI ATTAAT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 987
Psp124BI GAGCTC 2 cut(s) 248, 974
Psp6I CCWGG 4 cut(s) 639, 1178, 1394, 1485
PspEI GGTNACC 1 cut(s) 1083
PspGI CCWGG 4 cut(s) 639, 1178, 1394, 1485
PspN4I GGNNCC 2 cut(s) 591, 1994
PspPI GGNCC 1 cut(s) 213
PspXI VCTCGAGB 1 cut(s) 1867
PstI CTGCAG 1 cut(s) 817
PstNI CAGNNNCTG 1 cut(s) 821
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 812
RgaI GCGATCGC 1 cut(s) 89
RsaI GTAC 9 cut(s) 532, 591, 777, 1132, 1230, 1271, 1646, 1760, 1846
RsaNI GTAC 9 cut(s) 531, 590, 776, 1131, 1229, 1270, 1645, 1759, 1845
RseI CAYNNNNRTG 2 cut(s) 480, 1987
SacI GAGCTC 2 cut(s) 248, 974
SaqAI TTAA 5 cut(s) 716, 1010, 1595, 1787, 2053
Sau3AI GATC 8 cut(s) 86, 269, 333, 370, 414, 757, 1819, 1858
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 4 cut(s) 758, 812, 1160, 1859
ScrFI CCNGG 4 cut(s) 641, 1180, 1396, 1487
SduI GDGCHC 5 cut(s) 248, 311, 974, 1302, 1729
SfaAI GCGATCGC 1 cut(s) 89
SfaNI GCATC 5 cut(s) 274, 887, 1025, 1512, 1807
SfcI CTRYAG 3 cut(s) 695, 813, 1824
Sfr274I CTCGAG 1 cut(s) 1867
SgfI GCGATCGC 1 cut(s) 89
SinI GGWCC 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 1867
SmiMI CAYNNNNRTG 2 cut(s) 480, 1987
SmlI CTYRAG 3 cut(s) 631, 1586, 1867
SmoI CTYRAG 3 cut(s) 631, 1586, 1867
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 7 cut(s) 160, 354, 909, 1697, 1796, 1961, 2016
SspI AATATT 3 cut(s) 124, 1641, 1665
SspMI CTAG 7 cut(s) 27, 345, 623, 1373, 1478, 1805, 1935
SstI GAGCTC 2 cut(s) 248, 974
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 4 cut(s) 639, 1178, 1394, 1485
StyI CCWWGG 3 cut(s) 216, 593, 1716
TaaI ACNGT 5 cut(s) 467, 530, 699, 1030, 1130
TaiI ACGT 2 cut(s) 60, 118
TaqI TCGA 5 cut(s) 242, 336, 1296, 1494, 1868
TaqII GACCGA 2 cut(s) 1071, 1599
TatI WGTACW 4 cut(s) 530, 1130, 1228, 1644
TauI GCSGC 2 cut(s) 1963, 2019
TfiI GAWTC 4 cut(s) 794, 1153, 1245, 2038
Tru1I TTAA 5 cut(s) 716, 1010, 1595, 1787, 2053
Tru9I TTAA 5 cut(s) 716, 1010, 1595, 1787, 2053
TscAI CASTG 2 cut(s) 472, 1345
TseFI GTSAC 6 cut(s) 473, 896, 1083, 1432, 1611, 2026
TseI GCWGC 9 cut(s) 179, 439, 809, 812, 866, 875, 1040, 1143, 1256
Tsp45I GTSAC 6 cut(s) 473, 896, 1083, 1432, 1611, 2026
TspRI CASTG 2 cut(s) 472, 1345
VpaK11BI GGWCC 1 cut(s) 213
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 3 cut(s) 922, 1211, 1789
XhoI CTCGAG 1 cut(s) 1867
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 7 cut(s) 27, 345, 623, 1373, 1478, 1805, 1935
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.