MD01G1079000.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002962
Physical Location & Seq
Forward (+)
18622090 .. 18624746
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1079000.v1.1.491

Sequence Viewer

Length: 2061 bp
ATGCCATGCAGATGCAGTTCACTAGTTATTCCTACCTCTCCGGCTTCCGGCACGACGGTAGACGTTTTCAAAGAGCACAACGGTCTCCGCCGGTCAAACTCCAACCAAGACCTTTACCGTCGTGCTACTATGCGGAGGTCTTATTCGGACAACCATCTTTGTTACCGCAGCAGTCGTATCCACGCTGCTGCAACAGAACCAAAACTAAAGAACAGCCGTTCGGGGATTTTCTCGTTTCAGTTTTCGAGTTCCATTCTCCCAAACTCGGTGAGATCATTCTCGTTTGATCCGGACACGAGTAATCATAGGAGCATAAAGGCGAAGGACATGAGTAAGGAGACGTCTGGGGAGAGCGCCAGTGAGGAGGAGAGCAAAAGAGCCAATTGGGTGGAGAGACTAGTGGAAATCAGAAGTAAATGGAGGGATAGGCAGAAAAAGGACGATGCGGATGAAGACAGCGTCTGTGAAGCGGGTGAAAATGGTGATTGTGAATGTGGCAGAGATGAGGGTGGTTGTGCGGTGGATTATACTGCAGAAGACGATGGGGGAGAAACCAGTCCTGAAATTTTCCGGAGGTTTTTGGTTCGAGTTCCATGGTCTGATACCAAGCTGCTTTCCAAGCTATCCTTCTTGTGCAACTTGGCCTATGCGATGCCACAAATCAAGGAAAATTATATAAAGAGATATTATGGCCTACGATTAGTAACAACTTCACTAGAGAAGAAGGCCGAAGATGAAATCAAAGCAAAACTAGATGACGACTCTAATTGTGTACCTGTAGCTGCCGAAGTAGCTAGTGAATCCAGTTCGGAGAAAGCTGTAGAAGCTGGGCAGAAGCGTCCATGCCGTCCTGCTGTTGCTTATAATATTGCTGCCTCAGCTGCATCTTATGTGCGGTCACGCAATCAGGACAGCCTATCACTTGGGCCCAAATCGCAGCAGGAGGGTGATGGCGACAATTCACGAGAGGAAGGAGGGTACTCACCTCGAAGCTATAAGCCAGAGGTGGCAGTTTCCATGGCAGCGTCAACAATGACAGCAGTGGTTGCAGCAGGGGAAAAGGAGAAACAAGAAACAGCAAAGGACCTTCGGTCACTCCAGTCCTCACCTTGTGAATGGTTTGTCTGTGATGATGCCAGTACGTATACTCGATGTTTCGTGATTCAGGGTTCCGACTCCCTTGCATCCTGGCAGGCAAACCTGTTCTTCGAACCTACCAAATTCGAGGGGACAGCTGTGCTTGTTCACAGAGGAATCTACGAAGCAGCGAAGGGAATGTACGAGCGATTTATTCCAGAAATAACTGAACACTTGAATAGACACGGTGAGCGAGCGAAGCTTCAGTTTACAGGCCACTCTCTTGGAGGAAGCCTTTCTCTCTTGGTCCACTTGATGCTGCTGAGTAGGAAGTGTGTAAAACCCTCTACTCTTCGGCCAGTTGTCACATTTGGCTCGCCATTTGTGTTCTGTGGAGGCCAGAAAGTACTCGACAGACTGGGAGTGGACGAGGGTCAAATCCATTCCGTGATGATGCATAGAGATATCGTCCCTAGAGCCTTCTCCTGCAACTACCCTAACTATGTGGCCACACTTCTCAAGCGTTTAAATTCATTTCGGTCACACCCTTGTCTAATAAAAAATAAAATATTATATTCTCCGATGGGCAAACTAATCATTCTCCAACCTGATGAGAATGAATCTCCTCCACACCCTCTACTTCCTCCGGGGAGTGCCCTCTATGCTTTGGACAAGACCCAACGTGGATTCTCTTCAAGTGTCCTTACGACTTTCTTGAACTCTCCCCATCCACTAGAAACCCTAAGTGATCCTACAGCTTATGGTTCAGACGGTACAATTCTACGAGACCATGACTCAAGCAACTATCTAAAGGCTGTGAATCAAGTCCTAAGGAAATACACAAGGACAGCTGTTCGGAAAGTGAGAAAAGAAAGGAATCTATTGTGGCCGCTACTTACTTCACCATCTCCACACTCCTGGAACCATGAAAGTAATCTGGAGAGCAGCGGGTTGGTAACCAAGGAGATACTCACAGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

687

Amino Acids

76.35

Weight (kDa)

8.31

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 386 - 523 6.3e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AatII GACGTC 1 cut(s) 344
AccI GTMKAC 2 cut(s) 60, 1145
AccIII TCCGGA 2 cut(s) 289, 570
AciI CCGC 9 cut(s) 88, 133, 166, 446, 470, 518, 895, 1967, 2025
AclWI GGATC 2 cut(s) 281, 1820
AcoI YGGCCR 3 cut(s) 1433, 1584, 1964
AcsI RAATTY 3 cut(s) 564, 1220, 1606
AcuI CTGAAG 1 cut(s) 1325
AcyI GRCGYC 1 cut(s) 341
AdeI CACNNNGTG 1 cut(s) 1112
AfaI GTAC 6 cut(s) 774, 980, 1141, 1280, 1485, 1852
AfiI CCNNNNNNNGG 2 cut(s) 47, 265
AgsI TTSAA 4 cut(s) 70, 1315, 1773, 1795
AhdI GACNNNNNGTC 1 cut(s) 1090
AhlI ACTAGT 2 cut(s) 22, 397
AjnI CCWGG 2 cut(s) 1187, 1994
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 4 cut(s) 89, 332, 388, 1857
AlwI GGATC 2 cut(s) 281, 1820
AlwNI CAGNNNCTG 1 cut(s) 462
Aor13HI TCCGGA 2 cut(s) 289, 570
AoxI GGCC 9 cut(s) 642, 691, 726, 926, 1351, 1433, 1474, 1584, 1964
ApaI GGGCCC 1 cut(s) 930
ApoI RAATTY 3 cut(s) 564, 1220, 1606
Asp700I GAANNNNTTC 1 cut(s) 1372
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 4 cut(s) 926, 927, 1084, 1384
AsuC2I CCSGG 1 cut(s) 1725
AsuHPI GGTGA 8 cut(s) 280, 485, 494, 959, 975, 1098, 1337, 1971
AsuII TTCGAA 1 cut(s) 1209
AvaII GGWCC 2 cut(s) 1084, 1384
AxyI CCTNAGG 1 cut(s) 1907
BaeGI GKGCMC 2 cut(s) 930, 1735
BalI TGGCCA 1 cut(s) 1586
BanII GRGCYC 1 cut(s) 930
BarI GAAGNNNNNNTAC 2 cut(s) 1262, 1294
BauI CACGAG 2 cut(s) 295, 963
BbsI GAAGAC 2 cut(s) 459, 543
Bbv12I GWGCWC 1 cut(s) 78
BbvCI CCTCAGC 1 cut(s) 877
BccI CCATC 6 cut(s) 162, 536, 944, 1654, 1812, 1990
BceAI ACGGC 2 cut(s) 201, 831
BcgI CGANNNNNNTGC 2 cut(s) 1163, 1197
BciT130I CCWGG 2 cut(s) 1189, 1996
BciVI GTATCC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 1725
BcoDI GTCTC 4 cut(s) 89, 332, 388, 1857
BcuI ACTAGT 2 cut(s) 22, 397
BfaI CTAG 7 cut(s) 23, 398, 716, 752, 795, 1551, 1811
BfmI CTRYAG 4 cut(s) 531, 777, 819, 1830
BfoI RGCGCY 1 cut(s) 357
BfuI GTATCC 1 cut(s) 188
BmcAI AGTACT 1 cut(s) 1485
Bme1390I CCNGG 3 cut(s) 1189, 1725, 1996
Bme18I GGWCC 2 cut(s) 1084, 1384
BmeRI GACNNNNNGTC 1 cut(s) 1090
BmgT120I GGNCC 4 cut(s) 926, 927, 1084, 1384
BmiI GGNNCC 3 cut(s) 928, 1171, 2000
BmrFI CCNGG 3 cut(s) 1189, 1725, 1996
BmrI ACTGGG 1 cut(s) 1505
BmsI GCATC 8 cut(s) 2, 433, 642, 893, 1123, 1193, 1383, 1521
BmuI ACTGGG 1 cut(s) 1505
BoxI GACNNNNGTC 1 cut(s) 1509
BpiI GAAGAC 2 cut(s) 459, 543
BpmI CTGGAG 2 cut(s) 1082, 2036
Bpu10I CCTNAGC 1 cut(s) 877
Bpu14I TTCGAA 1 cut(s) 1209
BpuEI CTTGAG 2 cut(s) 1580, 1858
BpuMI CCSGG 1 cut(s) 1725
BsaAI YACGTR 1 cut(s) 1143
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 2 cut(s) 89, 1857
BsaJI CCNNGG 4 cut(s) 593, 1017, 1724, 2037
BsaWI WCCGGW 2 cut(s) 289, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 265
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 7 cut(s) 357, 555, 804, 1099, 1137, 1436, 1500
Bse21I CCTNAGG 1 cut(s) 1907
BseAI TCCGGA 2 cut(s) 289, 570
BseBI CCWGG 2 cut(s) 1189, 1996
BseDI CCNNGG 4 cut(s) 593, 1017, 1724, 2037
BseGI GGATG 3 cut(s) 454, 1184, 1804
BseLI CCNNNNNNNGG 2 cut(s) 47, 265
BseMII CTCAG 2 cut(s) 891, 1391
BseNI ACTGG 7 cut(s) 357, 555, 804, 1099, 1137, 1436, 1500
BseRI GAGGAG 3 cut(s) 377, 380, 1692
BseSI GKGCMC 2 cut(s) 930, 1735
BseYI CCCAGC 1 cut(s) 827
BshFI GGCC 9 cut(s) 644, 693, 728, 928, 1353, 1435, 1476, 1586, 1966
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 6 cut(s) 41, 48, 91, 290, 571, 1724
BslFI GGGAC 2 cut(s) 1243, 1532
BslI CCNNNNNNNGG 2 cut(s) 47, 265
BsmAI GTCTC 4 cut(s) 89, 332, 388, 1857
BsmBI CGTCTC 1 cut(s) 332
BsmFI GGGAC 2 cut(s) 1243, 1532
BsnI GGCC 9 cut(s) 644, 693, 728, 928, 1353, 1435, 1476, 1586, 1966
Bso31I GGTCTC 2 cut(s) 89, 1857
Bsp119I TTCGAA 1 cut(s) 1209
Bsp120I GGGCCC 1 cut(s) 926
Bsp1286I GDGCHC 3 cut(s) 78, 930, 1735
Bsp13I TCCGGA 2 cut(s) 289, 570
Bsp143I GATC 3 cut(s) 272, 286, 1825
Bsp19I CCATGG 2 cut(s) 593, 1017
BspACI CCGC 9 cut(s) 88, 133, 166, 446, 470, 518, 895, 1967, 2025
BspANI GGCC 9 cut(s) 644, 693, 728, 928, 1353, 1435, 1476, 1586, 1966
BspCNI CTCAG 2 cut(s) 890, 1392
BspEI TCCGGA 2 cut(s) 289, 570
BspLI GGNNCC 3 cut(s) 928, 1171, 2000
BspMAI CTGCAG 1 cut(s) 535
BspPI GGATC 2 cut(s) 281, 1820
BspT104I TTCGAA 1 cut(s) 1209
BspTNI GGTCTC 2 cut(s) 89, 1857
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 7 cut(s) 357, 555, 804, 1099, 1137, 1436, 1500
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 4 cut(s) 593, 1017, 1724, 2037
BssMI GATC 3 cut(s) 272, 286, 1825
BssNAI GTATAC 1 cut(s) 1146
BssNI GRCGYC 1 cut(s) 341
BssSI CACGAG 2 cut(s) 295, 963
BssT1I CCWWGG 3 cut(s) 593, 1017, 2037
Bst1107I GTATAC 1 cut(s) 1146
Bst2BI CACGAG 2 cut(s) 295, 963
Bst2UI CCWGG 2 cut(s) 1189, 1996
Bst4CI ACNGT 5 cut(s) 58, 83, 119, 1325, 1850
Bst6I CTCTTC 2 cut(s) 1434, 1774
BstACI GRCGYC 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBAI YACGTR 1 cut(s) 1143
BstBI TTCGAA 1 cut(s) 1209
BstC8I GCNNGC 3 cut(s) 1194, 1332, 1454
BstDEI CTNAG 4 cut(s) 877, 1400, 1820, 1907
BstDSI CCRYGG 2 cut(s) 593, 1017
BstEII GGTNACC 1 cut(s) 2032
BstF5I GGATG 3 cut(s) 454, 1184, 1804
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 3 cut(s) 275, 289, 1828
BstMAI GTCTC 4 cut(s) 89, 332, 388, 1857
BstMBI GATC 3 cut(s) 272, 286, 1825
BstMWI GCNNNNNNNGC 9 cut(s) 619, 791, 824, 878, 881, 934, 1046, 1336, 1739
BstNI CCWGG 2 cut(s) 1189, 1996
BstPAI GACNNNNGTC 1 cut(s) 1509
BstPI GGTNACC 1 cut(s) 2032
BstSCI CCNGG 3 cut(s) 1187, 1723, 1994
BstSFI CTRYAG 4 cut(s) 531, 777, 819, 1830
BstSLI GKGCMC 2 cut(s) 930, 1735
BstSNI TACGTA 1 cut(s) 1143
BstV2I GAAGAC 2 cut(s) 459, 543
BstXI CCANNNNNNTGG 3 cut(s) 388, 1361, 1995
BstZ17I GTATAC 1 cut(s) 1146
Bsu36I CCTNAGG 1 cut(s) 1907
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 9 cut(s) 644, 693, 728, 928, 1353, 1435, 1476, 1586, 1966
BtgI CCRYGG 2 cut(s) 593, 1017
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 3 cut(s) 454, 1184, 1804
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 2 cut(s) 364, 1047
Cac8I GCNNGC 3 cut(s) 1194, 1332, 1454
CaiI CAGNNNCTG 1 cut(s) 462
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 4 cut(s) 926, 927, 1084, 1384
CseI GACGC 3 cut(s) 448, 827, 1014
Csp6I GTAC 6 cut(s) 773, 979, 1140, 1279, 1484, 1851
CviAII CATG 7 cut(s) 6, 328, 594, 843, 1018, 1868, 2003
CviQI GTAC 6 cut(s) 773, 979, 1140, 1279, 1484, 1851
DdeI CTNAG 4 cut(s) 877, 1400, 1820, 1907
DpnI GATC 3 cut(s) 274, 288, 1827
DpnII GATC 3 cut(s) 272, 286, 1825
DraI TTTAAA 1 cut(s) 1605
DraIII CACNNNGTG 1 cut(s) 1112
DriI GACNNNNNGTC 1 cut(s) 1090
EaeI YGGCCR 3 cut(s) 1433, 1584, 1964
Eam1104I CTCTTC 2 cut(s) 1434, 1774
Eam1105I GACNNNNNGTC 1 cut(s) 1090
EarI CTCTTC 2 cut(s) 1434, 1774
EciI GGCGGA 1 cut(s) 77
Eco105I TACGTA 1 cut(s) 1143
Eco130I CCWWGG 3 cut(s) 593, 1017, 2037
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 2 cut(s) 89, 1857
Eco32I GATATC 1 cut(s) 1543
Eco47I GGWCC 2 cut(s) 1084, 1384
Eco57I CTGAAG 1 cut(s) 1325
Eco81I CCTNAGG 1 cut(s) 1907
Eco91I GGTNACC 1 cut(s) 2032
EcoO109I RGGNCCY 1 cut(s) 1084
EcoO65I GGTNACC 1 cut(s) 2032
EcoRII CCWGG 2 cut(s) 1187, 1994
EcoRV GATATC 1 cut(s) 1543
EcoT14I CCWWGG 3 cut(s) 593, 1017, 2037
EcoT22I ATGCAT 1 cut(s) 1536
EcoT38I GRGCYC 1 cut(s) 930
ErhI CCWWGG 3 cut(s) 593, 1017, 2037
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 7 cut(s) 9, 331, 597, 846, 1021, 1871, 2006
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 2 cut(s) 1243, 1532
FatI CATG 7 cut(s) 5, 327, 593, 842, 1017, 1867, 2002
FauI CCCGC 2 cut(s) 463, 2018
FblI GTMKAC 2 cut(s) 60, 1145
FokI GGATG 3 cut(s) 461, 1171, 1791
FriOI GRGCYC 1 cut(s) 930
FspBI CTAG 7 cut(s) 23, 398, 716, 752, 795, 1551, 1811
GlaI GCGC 1 cut(s) 355
GsaI CCCAGC 1 cut(s) 831
GsuI CTGGAG 2 cut(s) 1082, 2036
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 9 cut(s) 644, 693, 728, 928, 1353, 1435, 1476, 1586, 1966
HapII CCGG 6 cut(s) 41, 48, 91, 290, 571, 1724
HgaI GACGC 3 cut(s) 448, 827, 1014
HhaI GCGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 7 cut(s) 9, 331, 597, 846, 1021, 1871, 2006
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HindIII AAGCTT 1 cut(s) 1337
HpaII CCGG 6 cut(s) 41, 48, 91, 290, 571, 1724
HphI GGTGA 8 cut(s) 280, 485, 494, 959, 975, 1098, 1337, 1971
Hpy166II GTNNAC 9 cut(s) 20, 61, 773, 1029, 1146, 1246, 1347, 1387, 1504
Hpy188I TCNGA 8 cut(s) 148, 410, 601, 811, 1174, 1659, 1846, 1935
Hpy188III TCNNGA 9 cut(s) 290, 560, 571, 908, 963, 1159, 1295, 1792, 2015
Hpy8I GTNNAC 9 cut(s) 20, 61, 773, 1029, 1146, 1246, 1347, 1387, 1504
Hpy99I CGWCG 2 cut(s) 58, 123
HpyAV CCTTC 7 cut(s) 316, 637, 718, 965, 1097, 1264, 1567
HpyCH4III ACNGT 5 cut(s) 58, 83, 119, 1325, 1850
HpyCH4IV ACGT 4 cut(s) 63, 341, 1142, 1759
HpyF10VI GCNNNNNNNGC 9 cut(s) 619, 791, 824, 878, 881, 934, 1046, 1336, 1739
HpyF3I CTNAG 4 cut(s) 877, 1400, 1820, 1907
HpySE526I ACGT 4 cut(s) 63, 341, 1142, 1759
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 7 cut(s) 9, 331, 597, 846, 1021, 1871, 2006
HspAI GCGC 1 cut(s) 354
Kpn2I TCCGGA 2 cut(s) 289, 570
Kzo9I GATC 3 cut(s) 272, 286, 1825
LmnI GCTCC 1 cut(s) 309
LweI GCATC 8 cut(s) 2, 433, 642, 893, 1123, 1193, 1383, 1521
MaeI CTAG 7 cut(s) 23, 398, 716, 752, 795, 1551, 1811
MaeII ACGT 4 cut(s) 63, 341, 1142, 1759
MaeIII GTNAC 7 cut(s) 161, 703, 897, 1092, 1441, 1617, 2032
MalI GATC 3 cut(s) 274, 288, 1827
MboI GATC 3 cut(s) 272, 286, 1825
MboII GAAGA 7 cut(s) 464, 548, 733, 743, 1198, 1421, 1761
MfeI CAATTG 1 cut(s) 382
MhlI GDGCHC 3 cut(s) 78, 930, 1735
MlsI TGGCCA 1 cut(s) 1586
MluCI AATT 8 cut(s) 382, 564, 670, 766, 958, 1220, 1606, 1854
MluNI TGGCCA 1 cut(s) 1586
MlyI GAGTC 3 cut(s) 755, 1169, 1865
MmeI TCCRAC 3 cut(s) 126, 1197, 1705
Mox20I TGGCCA 1 cut(s) 1586
Mph1103I ATGCAT 1 cut(s) 1536
MroI TCCGGA 2 cut(s) 289, 570
MroXI GAANNNNTTC 1 cut(s) 1372
MscI TGGCCA 1 cut(s) 1586
MseI TTAA 2 cut(s) 1604, 2059
MslI CAYNNNNRTG 1 cut(s) 10
Msp20I TGGCCA 1 cut(s) 1586
MspA1I CMGCKG 4 cut(s) 881, 1235, 1928, 2025
MspI CCGG 6 cut(s) 41, 48, 91, 290, 571, 1724
MspR9I CCNGG 3 cut(s) 1189, 1725, 1996
MunI CAATTG 1 cut(s) 382
MvaI CCWGG 2 cut(s) 1189, 1996
MwoI GCNNNNNNNGC 9 cut(s) 619, 791, 824, 878, 881, 934, 1046, 1336, 1739
NciI CCSGG 1 cut(s) 1725
NcoI CCATGG 2 cut(s) 593, 1017
NdeII GATC 3 cut(s) 272, 286, 1825
NlaIII CATG 7 cut(s) 9, 331, 597, 846, 1021, 1871, 2006
NlaIV GGNNCC 3 cut(s) 928, 1171, 2000
NmuCI GTSAC 4 cut(s) 897, 1092, 1441, 1617
NsiI ATGCAT 1 cut(s) 1536
NspV TTCGAA 1 cut(s) 1209
PdmI GAANNNNTTC 1 cut(s) 1372
PfeI GAWTC 7 cut(s) 800, 1162, 1254, 1697, 1764, 1897, 1954
PflFI GACNNNGTC 1 cut(s) 458
PfoI TCCNGGA 1 cut(s) 1994
PleI GAGTC 3 cut(s) 755, 1169, 1865
PpsI GAGTC 3 cut(s) 755, 1169, 1865
Ppu21I YACGTR 1 cut(s) 1143
PpuMI RGGWCCY 1 cut(s) 1084
PshAI GACNNNNGTC 1 cut(s) 1509
PsiI TTATAA 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 2 cut(s) 1187, 1994
PspEI GGTNACC 1 cut(s) 2032
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 2 cut(s) 1187, 1994
PspN4I GGNNCC 3 cut(s) 928, 1171, 2000
PspOMI GGGCCC 1 cut(s) 926
PspPI GGNCC 4 cut(s) 926, 927, 1084, 1384
PspPPI RGGWCCY 1 cut(s) 1084
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 462
PsyI GACNNNGTC 1 cut(s) 458
PvuII CAGCTG 3 cut(s) 881, 1235, 1928
RsaI GTAC 6 cut(s) 774, 980, 1141, 1280, 1485, 1852
RsaNI GTAC 6 cut(s) 773, 979, 1140, 1279, 1484, 1851
RseI CAYNNNNRTG 1 cut(s) 10
SaqAI TTAA 2 cut(s) 1604, 2059
Sau3AI GATC 3 cut(s) 272, 286, 1825
Sau96I GGNCC 4 cut(s) 926, 927, 1084, 1384
ScaI AGTACT 1 cut(s) 1485
SchI GAGTC 3 cut(s) 755, 1169, 1865
ScrFI CCNGG 3 cut(s) 1189, 1725, 1996
SduI GDGCHC 3 cut(s) 78, 930, 1735
SfaNI GCATC 8 cut(s) 2, 433, 642, 893, 1123, 1193, 1383, 1521
SfcI CTRYAG 4 cut(s) 531, 777, 819, 1830
SfuI TTCGAA 1 cut(s) 1209
SinI GGWCC 2 cut(s) 1084, 1384
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 2 cut(s) 1595, 1873
SmoI CTYRAG 2 cut(s) 1595, 1873
SnaBI TACGTA 1 cut(s) 1143
SpeI ACTAGT 2 cut(s) 22, 397
Sse9I AATT 8 cut(s) 382, 564, 670, 766, 958, 1220, 1606, 1854
SsiI CCGC 9 cut(s) 88, 133, 166, 446, 470, 518, 895, 1967, 2025
SspI AATATT 2 cut(s) 868, 1647
SspMI CTAG 7 cut(s) 23, 398, 716, 752, 795, 1551, 1811
StyD4I CCNGG 3 cut(s) 1187, 1723, 1994
StyI CCWWGG 3 cut(s) 593, 1017, 2037
TaaI ACNGT 5 cut(s) 58, 83, 119, 1325, 1850
TaiI ACGT 4 cut(s) 66, 344, 1145, 1762
TaqI TCGA 7 cut(s) 245, 586, 988, 1150, 1209, 1224, 1488
TaqII GACCGA 2 cut(s) 1080, 1605
TasI AATT 8 cut(s) 382, 564, 670, 766, 958, 1220, 1606, 1854
TatI WGTACW 1 cut(s) 1483
TauI GCSGC 1 cut(s) 1969
TfiI GAWTC 7 cut(s) 800, 1162, 1254, 1697, 1764, 1897, 1954
Tru1I TTAA 2 cut(s) 1604, 2059
Tru9I TTAA 2 cut(s) 1604, 2059
TscAI CASTG 2 cut(s) 364, 1047
TseFI GTSAC 4 cut(s) 897, 1092, 1441, 1617
Tsp45I GTSAC 4 cut(s) 897, 1092, 1441, 1617
TspDTI ATGAA 5 cut(s) 465, 750, 1599, 1710, 2019
TspGWI ACGGA 1 cut(s) 1513
TspRI CASTG 2 cut(s) 364, 1047
Tth111I GACNNNGTC 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 1084, 1384
XapI RAATTY 3 cut(s) 564, 1220, 1606
XmiI GTMKAC 2 cut(s) 60, 1145
XmnI GAANNNNTTC 1 cut(s) 1372
XspI CTAG 7 cut(s) 23, 398, 716, 752, 795, 1551, 1811
ZraI GACGTC 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 1485
Zsp2I ATGCAT 1 cut(s) 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.