RLG00000024940

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002962
Physical Location & Seq
Forward (+)
40526069 .. 40528257
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024940

Sequence Viewer

Length: 1683 bp
ATGGCATTGACTTCATTAGCTATTCCTAGCTCTCTGGCTTCATCAACAAAATATATCCTCAAAGAGTACAATGACCTTCACCAGTCACACTCTAGCAGAGACCTATGCAATCTTGCTAGTATCCAGGGAAGGTTTTCTTCAGCTGATAACCAGATTTGTTATTGCATCAACCGTATCCATGCAACATCAACAGAACCAGAACCAAAGAAGAGTCCATTCAAGCAAATTTCCTGGTTTCAAAGTCGGGGTGCTATCATTCCAAATTTGCTACGATCATATTCATTGGATCCAAACTCGAGTAAGCATATGAACATAGTGGAGAATGACAGAAATGAGGTGGAGAGCTCTCGGGAGAGCAGTGAAGCGGAGGAACTAAAGAGAACCAGTTGGGTGGAGAGTCTTGTGGAACTCGGCAGAAACAACTGGAAGAATATTTGGCAGCAAAAAGTGGTTGGAGGTGAAGACAACGACTGTGACGAGGAAAGGAAATGCAGATCATATGATCGTGACCTTTTCTCGAGATTTTTGGCCACGGATTTAAAGAGATACTATGGCCTACACTTTGTAACATCTTCATTAGATAAGCAAGCAAAGGCAGATGGAATTGTGAGTATCAAACCAAAACTTGATCAGGACTCTACTGCAGTGAATTCTATGGCCTCAGCTGCAGCTTATCTCCGCTCAGTAAGCAAATCACAGCAGGAGGTTGCTGGAGAGAATTCATGCAAAAATGGAGGGAGCTCACCCCAAAACCACAAGTCAGAGACAGATGCTTCCCTTACAGCATCAACAATGACGTTGATGCTCGCAGCAGGTAATGAGGAAAAGCAAGGAGCAGCAAAGGATATTCAGTCACTGCACTCCTTACCTTGTGAGTGGTTTGTTTGTGATGATCCCACTACATATACTCGTTTCTTTGTGATTCAGGGTATAGACTCCCTTGCATCCTGGCAGGCATATTTCTTATTTGAACCCATTAAATTTGAGGGAACTAGTGTTCTTGTTCACCGAGGAATTTATGAACTGGCAAAGGAAATTTACCAGCAGTTCAAACCCAAAATAATTGACCACTTGGCAAGATATGGGGAGCAAGCGAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTTTCTGTTATAGTCCACTTGATGCTACTGACTAGGAAGCTCGTCAAACCCTCAACTCTTCTGCCTGATGTCACATTTGGCTCCCCATATGTGTTCTGTGGAGGCCAGAAAATATTTGTGCAACTAGGATTGGATGAGTATGATCACATCCGTTGTGTTATGCTGCACAGAGATATTGTCCCTAGATCTTTCTCCTCTAACTACCCTAATCATGTTGCAGAAGTTCTTAAGCGTTCACATGGTTCATTTGAGTCACACCCTTGTCTAACAAAGAATAAGATATTGTACTCTCCTATGGGTAGACTATTTATCGTCCAACCTGATGAAAAGTCATCTCCCCCACACCCTCTACTCCCTTCAGGGAGCTCTCTCTATGCTTTGGACAGTACCCAATGTGGATTCTCCTCGAGTGTCTTGGGGGCCTTTTTGAATTCCCCACATCCACTAGAAACACTAAGTGATCCTACAGCGTATGGTTCGGAAGGTACAATTCTAAGGGACCATGACTCGAGCAACTATCTAAAGGCTGTGAATGGGGTCCTAAGGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

561

Amino Acids

62.46

Weight (kDa)

6.68

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 306 - 442 2.1e-20 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 803
AccBSI CCGCTC 1 cut(s) 681
AccI GTMKAC 1 cut(s) 1432
AciI CCGC 2 cut(s) 365, 679
AclWI GGATC 4 cut(s) 281, 294, 887, 1586
AcoI YGGCCR 2 cut(s) 528, 1111
AcsI RAATTY 8 cut(s) 225, 262, 649, 718, 980, 1014, 1035, 1561
AcuI CTGAAG 2 cut(s) 123, 1473
AdeI CACNNNGTG 1 cut(s) 1589
AfaI GTAC 4 cut(s) 68, 1418, 1519, 1618
AflII CTTAAG 1 cut(s) 1358
AgsI TTSAA 6 cut(s) 220, 239, 971, 1051, 1103, 1561
AhlI ACTAGT 1 cut(s) 992
AjnI CCWGG 3 cut(s) 123, 230, 947
AloI GAACNNNNNNTCC 2 cut(s) 981, 1013
Alw21I GWGCWC 3 cut(s) 347, 743, 1499
Alw26I GTCTC 2 cut(s) 93, 758
AlwI GGATC 4 cut(s) 281, 294, 887, 1586
AlwNI CAGNNNCTG 1 cut(s) 856
Ama87I CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
AoxI GGCC 6 cut(s) 528, 553, 657, 1111, 1234, 1551
ApeKI GCWGC 6 cut(s) 439, 665, 668, 809, 836, 1294
ApoI RAATTY 8 cut(s) 225, 262, 649, 718, 980, 1014, 1035, 1561
Asp700I GAANNNNTTC 2 cut(s) 133, 1132
AspS9I GGNCC 3 cut(s) 1551, 1630, 1669
AsuHPI GGTGA 4 cut(s) 71, 470, 735, 998
AvaI CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
AvaII GGWCC 2 cut(s) 1630, 1669
AxyI CCTNAGG 1 cut(s) 1673
BalI TGGCCA 2 cut(s) 530, 1113
BamHI GGATCC 1 cut(s) 286
BanII GRGCYC 3 cut(s) 347, 743, 1499
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 3 cut(s) 347, 743, 1499
BbvCI CCTCAGC 1 cut(s) 661
BbvI GCAGC 6 cut(s) 451, 652, 680, 821, 848, 1281
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 3 cut(s) 125, 232, 949
BciVI GTATCC 2 cut(s) 131, 185
BclI TGATCA 2 cut(s) 628, 1273
BcoDI GTCTC 2 cut(s) 93, 758
BcuI ACTAGT 1 cut(s) 992
BfaI CTAG 8 cut(s) 27, 93, 117, 993, 1164, 1256, 1314, 1577
BfmI CTRYAG 4 cut(s) 642, 666, 1596, 1679
BfrI CTTAAG 1 cut(s) 1358
BfuAI ACCTGC 1 cut(s) 803
BfuI GTATCC 2 cut(s) 131, 185
BglII AGATCT 1 cut(s) 1316
BisI GCNGC 6 cut(s) 440, 666, 669, 810, 837, 1295
BlsI GCNGC 6 cut(s) 441, 667, 670, 811, 838, 1296
Bme1390I CCNGG 3 cut(s) 125, 232, 949
Bme18I GGWCC 2 cut(s) 1630, 1669
BmeT110I CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
BmgT120I GGNCC 3 cut(s) 1551, 1630, 1669
BmiI GGNNCC 5 cut(s) 288, 1213, 1552, 1631, 1670
BmrFI CCNGG 3 cut(s) 125, 232, 949
BmsI GCATC 6 cut(s) 174, 760, 792, 794, 953, 1143
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 1 cut(s) 661
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 3 cut(s) 124, 531, 1009
Bse1I ACTGG 5 cut(s) 82, 384, 428, 1029, 1114
Bse21I CCTNAGG 1 cut(s) 1673
BseBI CCWGG 3 cut(s) 125, 232, 949
BseDI CCNNGG 3 cut(s) 124, 531, 1009
BseGI GGATG 4 cut(s) 944, 1270, 1278, 1570
BseMII CTCAG 2 cut(s) 675, 696
BseNI ACTGG 5 cut(s) 82, 384, 428, 1029, 1114
BseRI GAGGAG 2 cut(s) 1315, 1525
BseXI GCAGC 6 cut(s) 451, 652, 680, 821, 848, 1281
BsgI GTGCAG 2 cut(s) 842, 1280
BshFI GGCC 6 cut(s) 530, 555, 659, 1113, 1236, 1553
BsiHKAI GWGCWC 3 cut(s) 347, 743, 1499
BsiHKCI CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
BslFI GGGAC 2 cut(s) 1295, 1643
BsmAI GTCTC 2 cut(s) 93, 758
BsmFI GGGAC 2 cut(s) 1295, 1643
BsnI GGCC 6 cut(s) 530, 555, 659, 1113, 1236, 1553
Bso31I GGTCTC 1 cut(s) 93
BsoBI CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
Bsp1286I GDGCHC 3 cut(s) 347, 743, 1499
Bsp143I GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
BspACI CCGC 2 cut(s) 365, 679
BspANI GGCC 6 cut(s) 530, 555, 659, 1113, 1236, 1553
BspCNI CTCAG 2 cut(s) 674, 695
BspLI GGNNCC 5 cut(s) 288, 1213, 1552, 1631, 1670
BspMAI CTGCAG 2 cut(s) 646, 670
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 4 cut(s) 281, 294, 887, 1586
BspTI CTTAAG 1 cut(s) 1358
BspTNI GGTCTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 681
BsrI ACTGG 5 cut(s) 82, 384, 428, 1029, 1114
BssECI CCNNGG 3 cut(s) 124, 531, 1009
BssMI GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
Bst2UI CCWGG 3 cut(s) 125, 232, 949
Bst4CI ACNGT 3 cut(s) 173, 473, 1517
Bst6I CTCTTC 2 cut(s) 203, 1194
BstAFI CTTAAG 1 cut(s) 1358
BstC8I GCNNGC 4 cut(s) 588, 807, 954, 1092
BstDEI CTNAG 5 cut(s) 661, 682, 1586, 1625, 1673
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 4 cut(s) 944, 1270, 1278, 1570
BstKTI GATC 9 cut(s) 275, 289, 497, 505, 631, 895, 1276, 1319, 1594
BstMAI GTCTC 2 cut(s) 93, 758
BstMBI GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
BstMWI GCNNNNNNNGC 3 cut(s) 665, 687, 1096
BstNI CCWGG 3 cut(s) 125, 232, 949
BstSCI CCNGG 3 cut(s) 123, 230, 947
BstSFI CTRYAG 4 cut(s) 642, 666, 1596, 1679
BstV1I GCAGC 6 cut(s) 451, 652, 680, 821, 848, 1281
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 2 cut(s) 286, 1316
BstXI CCANNNNNNTGG 2 cut(s) 391, 1121
BstYI RGATCY 2 cut(s) 286, 1316
Bsu36I CCTNAGG 1 cut(s) 1673
BsuI GTATCC 2 cut(s) 131, 185
BsuRI GGCC 6 cut(s) 530, 555, 659, 1113, 1236, 1553
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 4 cut(s) 944, 1270, 1278, 1570
BtsI GCAGTG 3 cut(s) 364, 651, 854
BtsIMutI CAGTG 3 cut(s) 364, 651, 854
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 4 cut(s) 588, 807, 954, 1092
CaiI CAGNNNCTG 1 cut(s) 856
Cfr13I GGNCC 3 cut(s) 1551, 1630, 1669
Csp6I GTAC 4 cut(s) 67, 1417, 1518, 1617
CviAII CATG 5 cut(s) 179, 723, 1343, 1370, 1634
CviQI GTAC 4 cut(s) 67, 1417, 1518, 1617
DdeI CTNAG 5 cut(s) 661, 682, 1586, 1625, 1673
DpnI GATC 9 cut(s) 274, 288, 496, 504, 630, 894, 1275, 1318, 1593
DpnII GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
DraI TTTAAA 1 cut(s) 540
DraIII CACNNNGTG 1 cut(s) 1589
EaeI YGGCCR 2 cut(s) 528, 1111
Eam1104I CTCTTC 2 cut(s) 203, 1194
EarI CTCTTC 2 cut(s) 203, 1194
Ecl136II GAGCTC 3 cut(s) 345, 741, 1497
Eco24I GRGCYC 3 cut(s) 347, 743, 1499
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 1630, 1669
Eco53kI GAGCTC 3 cut(s) 345, 741, 1497
Eco57I CTGAAG 2 cut(s) 123, 1473
Eco81I CCTNAGG 1 cut(s) 1673
Eco88I CYCGRG 5 cut(s) 295, 348, 517, 1537, 1639
EcoICRI GAGCTC 3 cut(s) 345, 741, 1497
EcoO109I RGGNCCY 2 cut(s) 1551, 1669
EcoRI GAATTC 3 cut(s) 649, 718, 1561
EcoRII CCWGG 3 cut(s) 123, 230, 947
EcoT38I GRGCYC 3 cut(s) 347, 743, 1499
FaeI CATG 5 cut(s) 182, 726, 1346, 1373, 1637
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 1083, 1115
FaqI GGGAC 2 cut(s) 1295, 1643
FatI CATG 5 cut(s) 178, 722, 1342, 1369, 1633
FauNDI CATATG 3 cut(s) 306, 499, 1219
FbaI TGATCA 2 cut(s) 628, 1273
FblI GTMKAC 1 cut(s) 1432
Fnu4HI GCNGC 6 cut(s) 440, 666, 669, 810, 837, 1295
FokI GGATG 4 cut(s) 931, 1265, 1277, 1557
FriOI GRGCYC 3 cut(s) 347, 743, 1499
Fsp4HI GCNGC 6 cut(s) 440, 666, 669, 810, 837, 1295
FspBI CTAG 8 cut(s) 27, 93, 117, 993, 1164, 1256, 1314, 1577
GluI GCNGC 6 cut(s) 440, 666, 669, 810, 837, 1295
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 6 cut(s) 530, 555, 659, 1113, 1236, 1553
Hin1II CATG 5 cut(s) 182, 726, 1346, 1373, 1637
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 8 cut(s) 211, 397, 635, 922, 935, 1382, 1530, 1637
HphI GGTGA 4 cut(s) 71, 470, 735, 998
Hpy166II GTNNAC 4 cut(s) 1006, 1147, 1367, 1433
Hpy188I TCNGA 2 cut(s) 763, 1612
Hpy188III TCNNGA 5 cut(s) 350, 506, 517, 519, 632
Hpy8I GTNNAC 4 cut(s) 1006, 1147, 1367, 1433
HpyAV CCTTC 4 cut(s) 86, 123, 1497, 1607
HpyCH4III ACNGT 3 cut(s) 173, 473, 1517
HpyCH4IV ACGT 1 cut(s) 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 665, 687, 1096
HpyF3I CTNAG 5 cut(s) 661, 682, 1586, 1625, 1673
HpySE526I ACGT 1 cut(s) 797
Hsp92II CATG 5 cut(s) 182, 726, 1346, 1373, 1637
Ksp22I TGATCA 2 cut(s) 628, 1273
Kzo9I GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
LmnI GCTCC 5 cut(s) 738, 833, 1087, 1217, 1494
Lsp1109I GCAGC 6 cut(s) 451, 652, 680, 821, 848, 1281
LweI GCATC 6 cut(s) 174, 760, 792, 794, 953, 1143
MaeI CTAG 8 cut(s) 27, 93, 117, 993, 1164, 1256, 1314, 1577
MaeII ACGT 1 cut(s) 797
MaeIII GTNAC 7 cut(s) 84, 473, 506, 565, 852, 1201, 1383
MalI GATC 9 cut(s) 274, 288, 496, 504, 630, 894, 1275, 1318, 1593
MbiI CCGCTC 1 cut(s) 681
MboI GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
MboII GAAGA 6 cut(s) 129, 220, 439, 473, 564, 1181
MflI RGATCY 2 cut(s) 286, 1316
MhlI GDGCHC 3 cut(s) 347, 743, 1499
MlsI TGGCCA 2 cut(s) 530, 1113
MluNI TGGCCA 2 cut(s) 530, 1113
MlyI GAGTC 6 cut(s) 220, 406, 629, 929, 1391, 1631
MmeI TCCRAC 2 cut(s) 433, 1471
Mox20I TGGCCA 2 cut(s) 530, 1113
MroXI GAANNNNTTC 2 cut(s) 133, 1132
MscI TGGCCA 2 cut(s) 530, 1113
MseI TTAA 3 cut(s) 539, 978, 1359
Msp20I TGGCCA 2 cut(s) 530, 1113
MspA1I CMGCKG 2 cut(s) 143, 665
MspCI CTTAAG 1 cut(s) 1358
MspR9I CCNGG 3 cut(s) 125, 232, 949
MvaI CCWGG 3 cut(s) 125, 232, 949
MwoI GCNNNNNNNGC 3 cut(s) 665, 687, 1096
NdeI CATATG 3 cut(s) 306, 499, 1219
NdeII GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
NlaIII CATG 5 cut(s) 182, 726, 1346, 1373, 1637
NlaIV GGNNCC 5 cut(s) 288, 1213, 1552, 1631, 1670
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 6 cut(s) 84, 473, 506, 852, 1201, 1383
PaeR7I CTCGAG 4 cut(s) 295, 517, 1537, 1639
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdmI GAANNNNTTC 2 cut(s) 133, 1132
PfeI GAWTC 2 cut(s) 922, 1530
PkrI GCNGC 6 cut(s) 441, 667, 670, 811, 838, 1296
PleI GAGTC 6 cut(s) 219, 405, 629, 929, 1390, 1631
PpsI GAGTC 6 cut(s) 219, 405, 629, 929, 1390, 1631
PpuMI RGGWCCY 1 cut(s) 1669
Psp124BI GAGCTC 3 cut(s) 347, 743, 1499
Psp5II RGGWCCY 1 cut(s) 1669
Psp6I CCWGG 3 cut(s) 123, 230, 947
PspGI CCWGG 3 cut(s) 123, 230, 947
PspN4I GGNNCC 5 cut(s) 288, 1213, 1552, 1631, 1670
PspPI GGNCC 3 cut(s) 1551, 1630, 1669
PspPPI RGGWCCY 1 cut(s) 1669
PspXI VCTCGAGB 3 cut(s) 295, 1537, 1639
PstI CTGCAG 2 cut(s) 646, 670
PstNI CAGNNNCTG 1 cut(s) 856
PsuI RGATCY 2 cut(s) 286, 1316
PvuII CAGCTG 2 cut(s) 143, 665
RsaI GTAC 4 cut(s) 68, 1418, 1519, 1618
RsaNI GTAC 4 cut(s) 67, 1417, 1518, 1617
SacI GAGCTC 3 cut(s) 347, 743, 1499
SaqAI TTAA 3 cut(s) 539, 978, 1359
SatI GCNGC 6 cut(s) 440, 666, 669, 810, 837, 1295
Sau3AI GATC 9 cut(s) 272, 286, 494, 502, 628, 892, 1273, 1316, 1591
Sau96I GGNCC 3 cut(s) 1551, 1630, 1669
SchI GAGTC 6 cut(s) 220, 406, 629, 929, 1391, 1631
ScrFI CCNGG 3 cut(s) 125, 232, 949
SduI GDGCHC 3 cut(s) 347, 743, 1499
SfaNI GCATC 6 cut(s) 174, 760, 792, 794, 953, 1143
SfcI CTRYAG 4 cut(s) 642, 666, 1596, 1679
Sfr274I CTCGAG 4 cut(s) 295, 517, 1537, 1639
SinI GGWCC 2 cut(s) 1630, 1669
SlaI CTCGAG 4 cut(s) 295, 517, 1537, 1639
SmlI CTYRAG 5 cut(s) 295, 517, 1358, 1537, 1639
SmoI CTYRAG 5 cut(s) 295, 517, 1358, 1537, 1639
SpeI ACTAGT 1 cut(s) 992
SsiI CCGC 2 cut(s) 365, 679
SspI AATATT 2 cut(s) 433, 1245
SspMI CTAG 8 cut(s) 27, 93, 117, 993, 1164, 1256, 1314, 1577
SstI GAGCTC 3 cut(s) 347, 743, 1499
StyD4I CCNGG 3 cut(s) 123, 230, 947
TaaI ACNGT 3 cut(s) 173, 473, 1517
TaiI ACGT 1 cut(s) 800
TaqI TCGA 4 cut(s) 296, 518, 1538, 1640
TatI WGTACW 2 cut(s) 66, 1416
TfiI GAWTC 2 cut(s) 922, 1530
Tru1I TTAA 3 cut(s) 539, 978, 1359
Tru9I TTAA 3 cut(s) 539, 978, 1359
TscAI CASTG 3 cut(s) 364, 651, 861
TseFI GTSAC 6 cut(s) 84, 473, 506, 852, 1201, 1383
TseI GCWGC 6 cut(s) 439, 665, 668, 809, 836, 1294
Tsp45I GTSAC 6 cut(s) 84, 473, 506, 852, 1201, 1383
TspDTI ATGAA 8 cut(s) 30, 270, 323, 564, 711, 1035, 1365, 1470
TspGWI ACGGA 2 cut(s) 548, 1271
TspRI CASTG 3 cut(s) 364, 651, 861
Vha464I CTTAAG 1 cut(s) 1358
VpaK11BI GGWCC 2 cut(s) 1630, 1669
XapI RAATTY 8 cut(s) 225, 262, 649, 718, 980, 1014, 1035, 1561
XhoI CTCGAG 4 cut(s) 295, 517, 1537, 1639
XmiI GTMKAC 1 cut(s) 1432
XmnI GAANNNNTTC 2 cut(s) 133, 1132
XspI CTAG 8 cut(s) 27, 93, 117, 993, 1164, 1256, 1314, 1577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.