Rh1AG282800

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002962
Physical Location & Seq
Forward (+)
51197653 .. 51204168
6516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG282800.1

Sequence Viewer

Length: 1674 bp
ATGGCCTGCAGTTCATTATCTATACCTAGCTCTCCGGCTTCAACGAGGACTATAGACGTCTTCAAGGAGCAGAGTGGTCTTCGGCGATCGCAATCCAGCAAAGACCTTTACAGACGTGCAAATATTCGGAGGTCTTATTCCGATAACCATCTTTGCTACTGCATTAGGCCTATTCATGCTGCGTCAGCCCAACCAAAACTAAAGAACAGCCGGTCCATGGGGTTTTTCCCCTTTCAATTTTCGAGCTCCATTCTACCAAACTCGGTTAGATCATTTTTGTTTGATGCAGACACGAGTAAGCATATGAGCACGGCAGAGAGGGAAATGGAAATGATCGATAACTCTAGGGAGAGCGGTGAAGAAGAAGAAGATCATGAGATAAAGAGAGCCAATTGGGTGGAGAGGCTTGTGGAGATCAGAAGAAAATGGAGTAATAGGCAGCAAAAAGAAGTTGGGGATGAAGACAGTGTTTGTGACGAAGGTGATGGTGATTGTGAATGCGGAGAGGGTGGTTGTTTAGTGGATTACAGTACAGATGAAGATGATGGAGAGATAACTTCAGAAAGTTTCCAAAGGTTATTGGTCAAGGTACCATGGTCTGATACCAAACAATTTTCTCAGCTAGCCTTCTTGAGCAACCTGGCATATGTAATACCATTGATAAAGGCCGATGATTTGAAGAGATATTACGGCCTACAGTTTGTAACATCTTCATTAGTAAAAAAAGCAGAGGAAGAGGAACTCAAAGAAGGAATTGATCAGGACTCTACTCGTGTACCTGTAACTGACGAGGAATCCACTTCAGAGGCAGCTGCAGACTCTGAGCAGAAGCATTCAATCCATCCATCTGTTGCTTACGAAATTGCTGCCTCAGCAGCATCTTATGTTCGCTCGCGTGACATGGATGGTGGTGATGTAGAGAATTCATTCAGAAGTGGAGACCAACAACAACAACGAGAGGAAGGAGGGAGCTCCCCCCGAAATTATAAGTCGGAGGTAGCTGTTTCCTTAACAGCATCAACGATGACAGTCGTGGTTGCAGCAGGGGAAAAACAAAAGCAAGAAACAGCACAAGACCTTCGGTCACCCCAATCTGCACCTTGTGAATGGTTTGTTTGTGATGACAACAGTACATATACTCGCTGCTTTGTGATTCAGGGTTCGGACTCCCTTGCGTCCTGGCAGGCAAACCTCTTCTTTGAACCTACAAAATTTGAGGGAACAAATGTACTTGTTCACAGAGGAATCTATGAAGCAGCAAAGGGGATGTACGAGCAATTCATGCCACAGATAATCGAGCACTTGGATAGATATGGAGAGCGTGCAAAGTTTCAGTTCACTGGTCATTCTCTTGGAGGAAGCCTCTCCCTTCTAGTCCACTTGATGTTACTGACCAGGAACTTTGTCAAGCCCTCCACTCTTCGGCCAGTTGTGACATTTGGCTCACCATTTGTGTTTTGTGGAGGCCAGAGAATACTAGACGACCTGAGTGTCGATGAGAACCAAATCCATTGCGTGATGATGCATAGAGATATTGTCCCAAGAGCCTTCTCCTGCAACTACCCTAACTATGTGGCTACGCTTCTCAAGCGTTTAAATGGTTCATTTCGATCACACCCTTGTCTGATAAAAAACGTAAGCATCACTATGAAAATTAACGGTATATGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

557

Amino Acids

62.66

Weight (kDa)

5.4

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 383 - 520 1.1e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
AasI GACNNNNNNGTC 1 cut(s) 1490
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 354
AccII CGCG 1 cut(s) 895
AciI CCGC 2 cut(s) 354, 501
AcoI YGGCCR 1 cut(s) 1424
AcsI RAATTY 2 cut(s) 922, 1211
AcuI CTGAAG 2 cut(s) 543, 786
AcyI GRCGYC 1 cut(s) 57
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 6 cut(s) 532, 591, 777, 1132, 1230, 1271
AfiI CCNNNNNNNGG 2 cut(s) 217, 1422
AgsI TTSAA 6 cut(s) 42, 64, 236, 679, 837, 1202
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 116
AjnI CCWGG 3 cut(s) 639, 1178, 1394
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 6 cut(s) 30, 246, 622, 812, 972, 1001
AluI AGCT 6 cut(s) 30, 246, 622, 812, 972, 1001
Alw21I GWGCWC 4 cut(s) 248, 311, 974, 1302
Alw26I GTCTC 1 cut(s) 933
AlwNI CAGNNNCTG 1 cut(s) 821
AoxI GGCC 6 cut(s) 3, 167, 666, 691, 1424, 1465
ApeKI GCWGC 9 cut(s) 179, 439, 809, 812, 866, 875, 1040, 1143, 1256
ApoI RAATTY 2 cut(s) 922, 1211
AsiSI GCGATCGC 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 926
Asp718I GGTACC 1 cut(s) 589
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 6 cut(s) 368, 494, 500, 923, 1077, 1437
AsuNHI GCTAGC 1 cut(s) 622
AvaII GGWCC 1 cut(s) 213
BaeI ACNNNNGTAYC 4 cut(s) 581, 614, 759, 792
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 248, 974
BarI GAAGNNNNNNTAC 2 cut(s) 671, 703
BauI CACGAG 2 cut(s) 292, 771
BbsI GAAGAC 3 cut(s) 52, 71, 468
Bbv12I GWGCWC 4 cut(s) 248, 311, 974, 1302
BbvCI CCTCAGC 1 cut(s) 871
BbvI GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BccI CCATC 6 cut(s) 156, 479, 539, 849, 853, 899
BceAI ACGGC 2 cut(s) 327, 706
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 3 cut(s) 641, 1180, 1396
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 1 cut(s) 933
BfaI CTAG 6 cut(s) 27, 345, 623, 1373, 1478, 1672
BfmI CTRYAG 4 cut(s) 7, 51, 695, 813
BisI GCNGC 9 cut(s) 180, 440, 810, 813, 867, 876, 1041, 1144, 1257
BlsI GCNGC 9 cut(s) 181, 441, 811, 814, 868, 877, 1042, 1145, 1258
Bme1390I CCNGG 3 cut(s) 641, 1180, 1396
Bme18I GGWCC 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 3 cut(s) 641, 1180, 1396
BmsI GCATC 5 cut(s) 274, 887, 1025, 1512, 1650
BmtI GCTAGC 1 cut(s) 626
BpiI GAAGAC 3 cut(s) 52, 71, 468
BplI GAGNNNNNCTC 2 cut(s) 1347, 1379
Bpu10I CCTNAGC 1 cut(s) 871
BpuEI CTTGAG 2 cut(s) 652, 1571
Bsa29I ATCGAT 1 cut(s) 336
BsaBI GATNNNNATC 2 cut(s) 91, 147
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 933
BsaJI CCNNGG 2 cut(s) 216, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 1422
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 1345, 1427
Bse3DI GCAATG 1 cut(s) 1510
Bse8I GATNNNNATC 2 cut(s) 91, 147
BseBI CCWGG 3 cut(s) 641, 1180, 1396
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 216, 593
BseGI GGATG 4 cut(s) 463, 841, 910, 1272
BseJI GATNNNNATC 2 cut(s) 91, 147
BseLI CCNNNNNNNGG 2 cut(s) 217, 1422
BseMI GCAATG 1 cut(s) 1510
BseMII CTCAG 4 cut(s) 632, 813, 885, 1478
BseNI ACTGG 2 cut(s) 1345, 1427
BseXI GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 895
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 6 cut(s) 5, 169, 668, 693, 1426, 1467
BshNI GGYRCC 1 cut(s) 589
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 248, 311, 974, 1302
BsiSI CCGG 2 cut(s) 35, 211
BslFI GGGAC 1 cut(s) 1523
BslI CCNNNNNNNGG 2 cut(s) 217, 1422
BsmAI GTCTC 1 cut(s) 933
BsmFI GGGAC 1 cut(s) 1523
BsmI GAATGC 2 cut(s) 503, 832
BsnI GGCC 6 cut(s) 5, 169, 668, 693, 1426, 1467
Bso31I GGTCTC 1 cut(s) 933
Bsp1286I GDGCHC 4 cut(s) 248, 311, 974, 1302
Bsp143I GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
Bsp19I CCATGG 2 cut(s) 216, 593
BspACI CCGC 2 cut(s) 354, 501
BspANI GGCC 6 cut(s) 5, 169, 668, 693, 1426, 1467
BspCNI CTCAG 4 cut(s) 631, 814, 884, 1479
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 895
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 591
BspMAI CTGCAG 2 cut(s) 11, 817
BspOI GCTAGC 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 933
BsrBI CCGCTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 1510
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 1345, 1427
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 2 cut(s) 216, 593
BssMI GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 2 cut(s) 292, 771
BssT1I CCWWGG 2 cut(s) 216, 593
Bst2BI CACGAG 2 cut(s) 292, 771
Bst2UI CCWGG 3 cut(s) 641, 1180, 1396
Bst4CI ACNGT 6 cut(s) 467, 530, 699, 1030, 1130, 1661
Bst6I CTCTTC 4 cut(s) 674, 729, 1199, 1425
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstC8I GCNNGC 5 cut(s) 7, 624, 893, 1185, 1323
BstDEI CTNAG 4 cut(s) 618, 822, 871, 1487
BstDSI CCRYGG 2 cut(s) 216, 593
BstEII GGTNACC 1 cut(s) 1083
BstF5I GGATG 4 cut(s) 463, 841, 910, 1272
BstFNI CGCG 1 cut(s) 895
BstKTI GATC 7 cut(s) 89, 272, 336, 373, 417, 760, 1613
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
BstNI CCWGG 3 cut(s) 641, 1180, 1396
BstPI GGTNACC 1 cut(s) 1083
BstSCI CCNGG 3 cut(s) 639, 1178, 1394
BstSFI CTRYAG 4 cut(s) 7, 51, 695, 813
BstUI CGCG 1 cut(s) 895
BstV1I GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
BstV2I GAAGAC 3 cut(s) 52, 71, 468
BstXI CCANNNNNNTGG 1 cut(s) 397
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 6 cut(s) 5, 169, 668, 693, 1426, 1467
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 216, 593
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 4 cut(s) 463, 841, 910, 1272
BtsIMutI CAGTG 2 cut(s) 472, 1338
Cac8I GCNNGC 5 cut(s) 7, 624, 893, 1185, 1323
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 2 cut(s) 171, 1164
Csp6I GTAC 6 cut(s) 531, 590, 776, 1131, 1229, 1270
CviAII CATG 6 cut(s) 176, 217, 374, 594, 901, 1282
CviQI GTAC 6 cut(s) 531, 590, 776, 1131, 1229, 1270
DdeI CTNAG 4 cut(s) 618, 822, 871, 1487
DpnI GATC 7 cut(s) 88, 271, 335, 372, 416, 759, 1612
DpnII GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
DraI TTTAAA 1 cut(s) 1596
DraIII CACNNNGTG 1 cut(s) 1103
DrdI GACNNNNNNGTC 1 cut(s) 1490
DriI GACNNNNNGTC 1 cut(s) 1081
DseDI GACNNNNNNGTC 1 cut(s) 1490
EaeI YGGCCR 1 cut(s) 1424
Eam1104I CTCTTC 4 cut(s) 674, 729, 1199, 1425
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 4 cut(s) 674, 729, 1199, 1425
Ecl136II GAGCTC 2 cut(s) 246, 972
Eco130I CCWWGG 2 cut(s) 216, 593
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 248, 974
Eco31I GGTCTC 1 cut(s) 933
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 246, 972
Eco57I CTGAAG 2 cut(s) 543, 786
Eco91I GGTNACC 1 cut(s) 1083
EcoICRI GAGCTC 2 cut(s) 246, 972
EcoO65I GGTNACC 1 cut(s) 1083
EcoRI GAATTC 1 cut(s) 922
EcoRII CCWGG 3 cut(s) 639, 1178, 1394
EcoT14I CCWWGG 2 cut(s) 216, 593
EcoT22I ATGCAT 1 cut(s) 1527
EcoT38I GRGCYC 2 cut(s) 248, 974
ErhI CCWWGG 2 cut(s) 216, 593
FaeI CATG 6 cut(s) 179, 220, 377, 597, 904, 1285
FaqI GGGAC 1 cut(s) 1523
FatI CATG 6 cut(s) 175, 216, 373, 593, 900, 1281
FauNDI CATATG 2 cut(s) 303, 646
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 9 cut(s) 180, 440, 810, 813, 867, 876, 1041, 1144, 1257
FokI GGATG 4 cut(s) 470, 828, 917, 1279
FriOI GRGCYC 2 cut(s) 248, 974
Fsp4HI GCNGC 9 cut(s) 180, 440, 810, 813, 867, 876, 1041, 1144, 1257
FspBI CTAG 6 cut(s) 27, 345, 623, 1373, 1478, 1672
GluI GCNGC 9 cut(s) 180, 440, 810, 813, 867, 876, 1041, 1144, 1257
HaeIII GGCC 6 cut(s) 5, 169, 668, 693, 1426, 1467
HapII CCGG 2 cut(s) 35, 211
HgaI GACGC 2 cut(s) 171, 1164
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 6 cut(s) 179, 220, 377, 597, 904, 1285
HinfI GANTC 6 cut(s) 764, 794, 818, 1153, 1166, 1245
HpaII CCGG 2 cut(s) 35, 211
HphI GGTGA 6 cut(s) 368, 494, 500, 923, 1077, 1437
Hpy166II GTNNAC 4 cut(s) 776, 1237, 1338, 1378
Hpy188III TCNNGA 3 cut(s) 374, 631, 761
Hpy8I GTNNAC 4 cut(s) 776, 1237, 1338, 1378
HpyAV CCTTC 7 cut(s) 473, 637, 743, 956, 1088, 1379, 1558
HpyCH4III ACNGT 6 cut(s) 467, 530, 699, 1030, 1130, 1661
HpyCH4IV ACGT 3 cut(s) 57, 115, 1635
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
HpyF3I CTNAG 4 cut(s) 618, 822, 871, 1487
HpySE526I ACGT 3 cut(s) 57, 115, 1635
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 6 cut(s) 179, 220, 377, 597, 904, 1285
KpnI GGTACC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
LmnI GCTCC 4 cut(s) 67, 251, 969, 977
Lsp1109I GCAGC 9 cut(s) 166, 451, 799, 821, 853, 887, 1052, 1130, 1268
LweI GCATC 5 cut(s) 274, 887, 1025, 1512, 1650
MaeI CTAG 6 cut(s) 27, 345, 623, 1373, 1478, 1672
MaeII ACGT 3 cut(s) 57, 115, 1635
MaeIII GTNAC 8 cut(s) 473, 703, 781, 896, 1083, 1386, 1432, 1667
MalI GATC 7 cut(s) 88, 271, 335, 372, 416, 759, 1612
MbiI CCGCTC 1 cut(s) 354
MboI GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
MfeI CAATTG 1 cut(s) 391
MhlI GDGCHC 4 cut(s) 248, 311, 974, 1302
MlyI GAGTC 3 cut(s) 758, 812, 1160
MmeI TCCRAC 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 1527
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 3 cut(s) 1010, 1595, 1656
MslI CAYNNNNRTG 1 cut(s) 1646
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 2 cut(s) 35, 211
MspR9I CCNGG 3 cut(s) 641, 1180, 1396
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 2 cut(s) 503, 832
MvaI CCWGG 3 cut(s) 641, 1180, 1396
MvnI CGCG 1 cut(s) 895
MwoI GCNNNNNNNGC 5 cut(s) 185, 872, 875, 1282, 1588
NcoI CCATGG 2 cut(s) 216, 593
NdeI CATATG 2 cut(s) 303, 646
NdeII GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
NheI GCTAGC 1 cut(s) 622
NlaIII CATG 6 cut(s) 179, 220, 377, 597, 904, 1285
NlaIV GGNNCC 1 cut(s) 591
NmuCI GTSAC 5 cut(s) 473, 896, 1083, 1432, 1667
NsiI ATGCAT 1 cut(s) 1527
PagI TCATGA 1 cut(s) 373
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 2 cut(s) 503, 832
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 3 cut(s) 794, 1153, 1245
PkrI GCNGC 9 cut(s) 181, 441, 811, 814, 868, 877, 1042, 1145, 1258
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 3 cut(s) 758, 812, 1160
PpsI GAGTC 3 cut(s) 758, 812, 1160
PsiI TTATAA 1 cut(s) 987
Psp124BI GAGCTC 2 cut(s) 248, 974
Psp6I CCWGG 3 cut(s) 639, 1178, 1394
PspEI GGTNACC 1 cut(s) 1083
PspGI CCWGG 3 cut(s) 639, 1178, 1394
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 213
PstI CTGCAG 2 cut(s) 11, 817
PstNI CAGNNNCTG 1 cut(s) 821
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 812
RgaI GCGATCGC 1 cut(s) 89
RsaI GTAC 6 cut(s) 532, 591, 777, 1132, 1230, 1271
RsaNI GTAC 6 cut(s) 531, 590, 776, 1131, 1229, 1270
RseI CAYNNNNRTG 1 cut(s) 1646
SacI GAGCTC 2 cut(s) 248, 974
SaqAI TTAA 3 cut(s) 1010, 1595, 1656
SatI GCNGC 9 cut(s) 180, 440, 810, 813, 867, 876, 1041, 1144, 1257
Sau3AI GATC 7 cut(s) 86, 269, 333, 370, 414, 757, 1610
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 3 cut(s) 758, 812, 1160
ScrFI CCNGG 3 cut(s) 641, 1180, 1396
SduI GDGCHC 4 cut(s) 248, 311, 974, 1302
SfaAI GCGATCGC 1 cut(s) 89
SfaNI GCATC 5 cut(s) 274, 887, 1025, 1512, 1650
SfcI CTRYAG 4 cut(s) 7, 51, 695, 813
SgfI GCGATCGC 1 cut(s) 89
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 1646
SmlI CTYRAG 2 cut(s) 631, 1586
SmoI CTYRAG 2 cut(s) 631, 1586
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 2 cut(s) 354, 501
SspI AATATT 1 cut(s) 124
SspMI CTAG 6 cut(s) 27, 345, 623, 1373, 1478, 1672
SstI GAGCTC 2 cut(s) 248, 974
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 3 cut(s) 639, 1178, 1394
StyI CCWWGG 2 cut(s) 216, 593
TaaI ACNGT 6 cut(s) 467, 530, 699, 1030, 1130, 1661
TaiI ACGT 3 cut(s) 60, 118, 1638
TaqI TCGA 5 cut(s) 242, 336, 1296, 1494, 1609
TaqII GACCGA 1 cut(s) 1071
TatI WGTACW 3 cut(s) 530, 1130, 1228
TfiI GAWTC 3 cut(s) 794, 1153, 1245
Tru1I TTAA 3 cut(s) 1010, 1595, 1656
Tru9I TTAA 3 cut(s) 1010, 1595, 1656
TscAI CASTG 2 cut(s) 472, 1345
TseFI GTSAC 5 cut(s) 473, 896, 1083, 1432, 1667
TseI GCWGC 9 cut(s) 179, 439, 809, 812, 866, 875, 1040, 1143, 1256
Tsp45I GTSAC 5 cut(s) 473, 896, 1083, 1432, 1667
TspDTI ATGAA 9 cut(s) 164, 474, 552, 702, 915, 1266, 1270, 1593, 1664
TspRI CASTG 2 cut(s) 472, 1345
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 2 cut(s) 922, 1211
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 6 cut(s) 27, 345, 623, 1373, 1478, 1672
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.