Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0006291

Physical Location & Seq

Genomic Coordinates Reverse (-)

10320520 .. 10322785

Gene Structure 2266 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g16410.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGGGCGAGAAAGTTGAAGGTGCGAAGAATGAGGGCGAGAAGAAGGCCGCCGACGCCGGAAAGAAGGTCGACGACGGCTCAAACACTGTCGTTTACAAGACCGATATGCATTGCGAAGGATGCGCCAAGAAAATCAAACGAGCTGTCAAAAACTTCCCCGGCGTTGAGCAAGTGAAGACAGATGCCGGAGCTAACAAGCTGACGGTGACCGGGAAAATGGACCCCGCCGCTCTTAAGGCGAGACTGGAGGAGAAGATTAAGAAGAAGGTCGACCTCGTCTCTCCTCAGCCGGCCAAAAAGGAAGGCGGTGGAGGCGGAGGAGACAAGAAGGCGGATGAAAAGTCCGAAAAGCCCTCGGAGAAGAAAGCCGAGGAGAAAAAGCCGGCCGATAAGAAGCCCATAGAGAAAGAGGCGGCTGCCCCCAAAGAGAGCACTGTTCCTTTGAAGATTCGGTTGCATTGCGAGGGTTGCATCCAGAAGATGCGAAGTAAAATCTTGAAATATAAAGGTGTTAGTACAGTGAGCTTTGACATGGCCAAGGATCTGGTCACGGTGGTGGGCACTATGGACACCAAAACTTTAGTTCCTTATCTTTCGGAGAAGTTCAAGAGACCAGTCGACGTGATCGCGCCGGCGAAGAAGGACGATGGCGCCGGAGCCAAGAAAGATGATAAGAAACCGGCTGCTGAAGGCGGCGAGAAGAAAGACGGCGGCGGTGACAAAAAGGAAGAAAAGAAAGAGGGAAAGAAGGAAGCTGCCCCCGCAGGCGAGAAGAAAGAGGAGAAAAAGGAAGCTGCTGCCGGCGGTGGCGGTGGAGGCGCGGTGGTGAACAAAATGGAGTACTCTGGTTACCCTTACGCGGCATCGACATCGTACTGGTACGACCCCGGACATGTTTACAACCACAACAAGTTTGTGATGGAGGCTCAGGAGCACCAGGCGCATGTTAACCAGGGCTACGGGCACTACGCAATGCCGATGGAGCATTATCCGGCGCCGCAGATGTTCAGCGACGAGAACCCCAACGGTTGTTCGGTGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

348

Amino Acids

37.42

Weight (kDa)

9.04

Isoelectric Point (pI)

36.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	32 - 88	1.9e-15	Heavy-metal-associated domain
HMA	PF00403	151 - 195	5.9e-09	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36950 AT5G03380 AT5G03380
fragaria_vesca	FvH4_6g16410 FvH4_6g16410
malus_domestica	MD04G1093300.v1.1 MD12G1113000.v1.1
prunus_persica	Prupe.6G232100_v2.0.a1 Prupe.6G232100_v2.0.a1
pyrus_communis	pycom04g08830 pycom12g11200
rosa_chinensis	RchiOBHm_Chr3g0470271
rosa_laevigata	RLG00000024284 RLG00000024290
rosa_multiflora	Rmu_co8443661.1_g000001 Rmu_sc0005600.1_g000019 Rmu_sc0011073.1_g000002
rosa_roxburghii	Rroxscaffold_6G00411000 Rroxscaffold_6G00411110
rosa_rugosa	Rorug03G0107700
rosa_samantha	Rh1AG099300 Rh3AG157500 Rh3BG181600 Rh3CG172400 Rh3DG193300
rosa_wichuraiana	Rw3G014860 Rw3G014980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	650, 994
AccBSI	CCGCTC	1 cut(s)	230
AccI	GTMKAC	3 cut(s)	69, 270, 618
AccII	CGCG	3 cut(s)	629, 821, 860
AclWI	GGATC	1 cut(s)	549
AcoI	YGGCCR	3 cut(s)	291, 384, 534
AcuI	CTGAAG	1 cut(s)	708
AcyI	GRCGYC	3 cut(s)	54, 651, 995
AfaI	GTAC	4 cut(s)	517, 842, 875, 881
AfiI	CCNNNNNNNGG	1 cut(s)	859
AflII	CTTAAG	1 cut(s)	233
AflIII	ACRYGT	1 cut(s)	892
AgsI	TTSAA	4 cut(s)	17, 445, 499, 607
AjiI	CACGTC	1 cut(s)	622
AjnI	CCWGG	2 cut(s)	936, 951
AleI	CACNNNNGTG	1 cut(s)	554
AluBI	AGCT	6 cut(s)	143, 191, 199, 525, 755, 794
AluI	AGCT	6 cut(s)	143, 191, 199, 525, 755, 794
Alw21I	GWGCWC	2 cut(s)	434, 936
Alw26I	GTCTC	4 cut(s)	235, 283, 315, 604
AlwI	GGATC	1 cut(s)	549
AoxI	GGCC	4 cut(s)	45, 291, 384, 534
ApeKI	GCWGC	5 cut(s)	416, 683, 755, 794, 797
ArsI	GACNNNNNNTTYG	2 cut(s)	129, 161
AspLEI	GCGC	6 cut(s)	125, 631, 653, 821, 943, 997
AspS9I	GGNCC	1 cut(s)	220
AsuC2I	CCSGG	3 cut(s)	159, 211, 888
AsuHPI	GGTGA	3 cut(s)	217, 728, 838
AvaII	GGWCC	1 cut(s)	220
BaeGI	GKGCMC	2 cut(s)	563, 966
BalI	TGGCCA	1 cut(s)	536
BanI	GGYRCC	2 cut(s)	650, 994
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	2 cut(s)	434, 936
BbvCI	CCTCAGC	1 cut(s)	285
BbvI	GCAGC	5 cut(s)	403, 670, 742, 781, 784
BccI	CCATC	3 cut(s)	641, 913, 973
BceAI	ACGGC	2 cut(s)	91, 724
BcgI	CGANNNNNNTGC	2 cut(s)	852, 886
BciT130I	CCWGG	2 cut(s)	938, 953
BcnI	CCSGG	3 cut(s)	159, 211, 888
BcoDI	GTCTC	4 cut(s)	235, 283, 315, 604
BfoI	RGCGCY	2 cut(s)	654, 998
BfrI	CTTAAG	1 cut(s)	233
BmcAI	AGTACT	1 cut(s)	842
Bme1390I	CCNGG	5 cut(s)	159, 211, 888, 938, 953
Bme18I	GGWCC	1 cut(s)	220
BmgBI	CACGTC	1 cut(s)	622
BmgT120I	GGNCC	1 cut(s)	220
BmiI	GGNNCC	4 cut(s)	222, 652, 658, 996
BmrFI	CCNGG	5 cut(s)	159, 211, 888, 938, 953
BmsI	GCATC	5 cut(s)	110, 172, 471, 480, 872
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	266
Bpu10I	CCTNAGC	2 cut(s)	285, 927
BpuMI	CCSGG	3 cut(s)	159, 211, 888
BsaHI	GRCGYC	3 cut(s)	54, 651, 995
BsaI	GGTCTC	1 cut(s)	604
BsaJI	CCNNGG	6 cut(s)	157, 354, 369, 537, 886, 952
BsaXI	ACNNNNNCTCC	2 cut(s)	807, 837
Bsc4I	CCNNNNNNNGG	1 cut(s)	859
Bse118I	RCCGGY	5 cut(s)	289, 382, 631, 679, 800
Bse1I	ACTGG	3 cut(s)	249, 614, 881
Bse3DI	GCAATG	3 cut(s)	109, 457, 978
BseBI	CCWGG	2 cut(s)	938, 953
BseDI	CCNNGG	6 cut(s)	157, 354, 369, 537, 886, 952
BseGI	GGATG	3 cut(s)	125, 340, 471
BseLI	CCNNNNNNNGG	1 cut(s)	859
BseMI	GCAATG	3 cut(s)	109, 457, 978
BseMII	CTCAG	2 cut(s)	299, 941
BseNI	ACTGG	3 cut(s)	249, 614, 881
BseRI	GAGGAG	5 cut(s)	263, 273, 333, 386, 794
BseSI	GKGCMC	2 cut(s)	563, 966
BseX3I	CGGCCG	1 cut(s)	384
BseXI	GCAGC	5 cut(s)	403, 670, 742, 781, 784
Bsh1236I	CGCG	3 cut(s)	629, 821, 860
Bsh1285I	CGRYCG	1 cut(s)	387
BshFI	GGCC	4 cut(s)	47, 293, 386, 536
BshNI	GGYRCC	2 cut(s)	650, 994
BsiEI	CGRYCG	1 cut(s)	387
BsiHKAI	GWGCWC	2 cut(s)	434, 936
BslI	CCNNNNNNNGG	1 cut(s)	859
BsmAI	GTCTC	4 cut(s)	235, 283, 315, 604
BsmBI	CGTCTC	1 cut(s)	283
BsnI	GGCC	4 cut(s)	47, 293, 386, 536
Bso31I	GGTCTC	1 cut(s)	604
Bsp1286I	GDGCHC	4 cut(s)	434, 563, 936, 966
Bsp143I	GATC	2 cut(s)	541, 624
BspANI	GGCC	4 cut(s)	47, 293, 386, 536
BspCNI	CTCAG	2 cut(s)	298, 940
BspFNI	CGCG	3 cut(s)	629, 821, 860
BspLI	GGNNCC	4 cut(s)	222, 652, 658, 996
BspPI	GGATC	1 cut(s)	549
BspT107I	GGYRCC	2 cut(s)	650, 994
BspTI	CTTAAG	1 cut(s)	233
BspTNI	GGTCTC	1 cut(s)	604
BsrBI	CCGCTC	1 cut(s)	230
BsrDI	GCAATG	3 cut(s)	109, 457, 978
BsrFI	RCCGGY	5 cut(s)	289, 382, 631, 679, 800
BsrI	ACTGG	3 cut(s)	249, 614, 881
BssAI	RCCGGY	5 cut(s)	289, 382, 631, 679, 800
BssECI	CCNNGG	6 cut(s)	157, 354, 369, 537, 886, 952
BssMI	GATC	2 cut(s)	541, 624
BssNI	GRCGYC	3 cut(s)	54, 651, 995
BssT1I	CCWWGG	1 cut(s)	537
Bst2UI	CCWGG	2 cut(s)	938, 953
Bst4CI	ACNGT	6 cut(s)	88, 205, 436, 520, 553, 1028
BstACI	GRCGYC	3 cut(s)	54, 651, 995
BstAFI	CTTAAG	1 cut(s)	233
BstC8I	GCNNGC	5 cut(s)	291, 384, 633, 766, 802
BstDEI	CTNAG	2 cut(s)	285, 927
BstEII	GGTNACC	2 cut(s)	205, 848
BstF5I	GGATG	3 cut(s)	125, 340, 471
BstFNI	CGCG	3 cut(s)	629, 821, 860
BstH2I	RGCGCY	2 cut(s)	654, 998
BstHHI	GCGC	6 cut(s)	125, 631, 653, 821, 943, 997
BstKTI	GATC	2 cut(s)	544, 627
BstMAI	GTCTC	4 cut(s)	235, 283, 315, 604
BstMBI	GATC	2 cut(s)	541, 624
BstMCI	CGRYCG	1 cut(s)	387
BstMWI	GCNNNNNNNGC	9 cut(s)	53, 120, 236, 312, 468, 761, 816, 940, 982
BstNI	CCWGG	2 cut(s)	938, 953
BstNSI	RCATGY	2 cut(s)	896, 947
BstPI	GGTNACC	2 cut(s)	205, 848
BstSCI	CCNGG	5 cut(s)	157, 209, 886, 936, 951
BstSLI	GKGCMC	2 cut(s)	563, 966
BstUI	CGCG	3 cut(s)	629, 821, 860
BstV1I	GCAGC	5 cut(s)	403, 670, 742, 781, 784
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	1 cut(s)	541
BstXI	CCANNNNNNTGG	1 cut(s)	544
BstYI	RGATCY	1 cut(s)	541
BstZI	CGGCCG	1 cut(s)	384
BsuRI	GGCC	4 cut(s)	47, 293, 386, 536
BtrI	CACGTC	1 cut(s)	622
BtsCI	GGATG	3 cut(s)	125, 340, 471
BtsIMutI	CAGTG	3 cut(s)	84, 432, 525
Cac8I	GCNNGC	5 cut(s)	291, 384, 633, 766, 802
CfoI	GCGC	6 cut(s)	125, 631, 653, 821, 943, 997
Cfr10I	RCCGGY	5 cut(s)	289, 382, 631, 679, 800
Cfr13I	GGNCC	1 cut(s)	220
CseI	GACGC	1 cut(s)	62
Csp6I	GTAC	4 cut(s)	516, 841, 874, 880
CviAII	CATG	3 cut(s)	532, 893, 944
CviQI	GTAC	4 cut(s)	516, 841, 874, 880
DdeI	CTNAG	2 cut(s)	285, 927
DinI	GGCGCC	2 cut(s)	652, 996
DpnI	GATC	2 cut(s)	543, 626
DpnII	GATC	2 cut(s)	541, 624
EaeI	YGGCCR	3 cut(s)	291, 384, 534
EagI	CGGCCG	1 cut(s)	384
EciI	GGCGGA	2 cut(s)	330, 347
EclXI	CGGCCG	1 cut(s)	384
Eco130I	CCWWGG	1 cut(s)	537
Eco31I	GGTCTC	1 cut(s)	604
Eco47I	GGWCC	1 cut(s)	220
Eco52I	CGGCCG	1 cut(s)	384
Eco57I	CTGAAG	1 cut(s)	708
Eco91I	GGTNACC	2 cut(s)	205, 848
EcoO65I	GGTNACC	2 cut(s)	205, 848
EcoRII	CCWGG	2 cut(s)	936, 951
EcoT14I	CCWWGG	1 cut(s)	537
EcoT22I	ATGCAT	1 cut(s)	111
EgeI	GGCGCC	2 cut(s)	652, 996
EheI	GGCGCC	2 cut(s)	652, 996
ErhI	CCWWGG	1 cut(s)	537
Esp3I	CGTCTC	1 cut(s)	283
FaeI	CATG	3 cut(s)	535, 896, 947
FaiI	YATR	7 cut(s)	107, 401, 504, 533, 566, 894, 945
FatI	CATG	3 cut(s)	531, 892, 943
FauI	CCCGC	2 cut(s)	232, 769
FblI	GTMKAC	3 cut(s)	69, 270, 618
FokI	GGATG	3 cut(s)	132, 347, 458
GlaI	GCGC	6 cut(s)	124, 630, 652, 820, 942, 996
GsuI	CTGGAG	1 cut(s)	266
HaeII	RGCGCY	2 cut(s)	654, 998
HaeIII	GGCC	4 cut(s)	47, 293, 386, 536
HgaI	GACGC	1 cut(s)	62
HhaI	GCGC	6 cut(s)	125, 631, 653, 821, 943, 997
Hin1I	GRCGYC	3 cut(s)	54, 651, 995
Hin1II	CATG	3 cut(s)	535, 896, 947
Hin6I	GCGC	6 cut(s)	123, 629, 651, 819, 941, 995
HinP1I	GCGC	6 cut(s)	123, 629, 651, 819, 941, 995
HincII	GTYRAC	4 cut(s)	70, 271, 619, 949
HindII	GTYRAC	4 cut(s)	70, 271, 619, 949
HinfI	GANTC	1 cut(s)	448
HpaI	GTTAAC	1 cut(s)	949
HphI	GGTGA	3 cut(s)	217, 728, 838
Hpy166II	GTNNAC	7 cut(s)	70, 94, 271, 619, 829, 898, 949
Hpy188I	TCNGA	3 cut(s)	346, 358, 598
Hpy188III	TCNNGA	4 cut(s)	475, 496, 607, 929
Hpy8I	GTNNAC	7 cut(s)	70, 94, 271, 619, 829, 898, 949
Hpy99I	CGWCG	5 cut(s)	56, 74, 77, 623, 1016
HpyCH4III	ACNGT	6 cut(s)	88, 205, 436, 520, 553, 1028
HpyCH4IV	ACGT	1 cut(s)	621
HpyCH4V	TGCA	3 cut(s)	109, 457, 471
HpyF10VI	GCNNNNNNNGC	9 cut(s)	53, 120, 236, 312, 468, 761, 816, 940, 982
HpyF3I	CTNAG	2 cut(s)	285, 927
HpySE526I	ACGT	1 cut(s)	621
Hsp92I	GRCGYC	3 cut(s)	54, 651, 995
Hsp92II	CATG	3 cut(s)	535, 896, 947
HspAI	GCGC	6 cut(s)	123, 629, 651, 819, 941, 995
KasI	GGCGCC	2 cut(s)	650, 994
KroI	GCCGGC	4 cut(s)	289, 382, 631, 800
KroNI	GCCGGC	4 cut(s)	291, 384, 633, 802
KspAI	GTTAAC	1 cut(s)	949
Kzo9I	GATC	2 cut(s)	541, 624
LmnI	GCTCC	4 cut(s)	188, 656, 931, 982
Lsp1109I	GCAGC	5 cut(s)	403, 670, 742, 781, 784
LweI	GCATC	5 cut(s)	110, 172, 471, 480, 872
MaeII	ACGT	1 cut(s)	621
MaeIII	GTNAC	4 cut(s)	205, 547, 716, 848
MalI	GATC	2 cut(s)	543, 626
MbiI	CCGCTC	1 cut(s)	230
MboI	GATC	2 cut(s)	541, 624
MflI	RGATCY	1 cut(s)	541
MhlI	GDGCHC	4 cut(s)	434, 563, 936, 966
MlsI	TGGCCA	1 cut(s)	536
MluNI	TGGCCA	1 cut(s)	536
Mly113I	GGCGCC	2 cut(s)	651, 995
Mox20I	TGGCCA	1 cut(s)	536
Mph1103I	ATGCAT	1 cut(s)	111
MreI	CGCCGGCG	1 cut(s)	631
MroNI	GCCGGC	4 cut(s)	289, 382, 631, 800
MscI	TGGCCA	1 cut(s)	536
MseI	TTAA	3 cut(s)	234, 258, 948
MslI	CAYNNNNRTG	1 cut(s)	554
Msp20I	TGGCCA	1 cut(s)	536
MspCI	CTTAAG	1 cut(s)	233
MspR9I	CCNGG	5 cut(s)	159, 211, 888, 938, 953
MvaI	CCWGG	2 cut(s)	938, 953
MvnI	CGCG	3 cut(s)	629, 821, 860
MwoI	GCNNNNNNNGC	9 cut(s)	53, 120, 236, 312, 468, 761, 816, 940, 982
NaeI	GCCGGC	4 cut(s)	291, 384, 633, 802
NarI	GGCGCC	2 cut(s)	651, 995
NciI	CCSGG	3 cut(s)	159, 211, 888
NdeII	GATC	2 cut(s)	541, 624
NgoMIV	GCCGGC	4 cut(s)	289, 382, 631, 800
NlaIII	CATG	3 cut(s)	535, 896, 947
NlaIV	GGNNCC	4 cut(s)	222, 652, 658, 996
NmeAIII	GCCGAG	1 cut(s)	394
NmuCI	GTSAC	3 cut(s)	205, 547, 716
NsiI	ATGCAT	1 cut(s)	111
NspI	RCATGY	2 cut(s)	896, 947
OliI	CACNNNNGTG	1 cut(s)	554
PciI	ACATGT	1 cut(s)	892
PdiI	GCCGGC	4 cut(s)	291, 384, 633, 802
PfeI	GAWTC	1 cut(s)	448
PflFI	GACNNNGTC	1 cut(s)	275
PluTI	GGCGCC	2 cut(s)	654, 998
PscI	ACATGT	1 cut(s)	892
Psp6I	CCWGG	2 cut(s)	936, 951
PspEI	GGTNACC	2 cut(s)	205, 848
PspGI	CCWGG	2 cut(s)	936, 951
PspN4I	GGNNCC	4 cut(s)	222, 652, 658, 996
PspPI	GGNCC	1 cut(s)	220
PsuI	RGATCY	1 cut(s)	541
PsyI	GACNNNGTC	1 cut(s)	275
RsaI	GTAC	4 cut(s)	517, 842, 875, 881
RsaNI	GTAC	4 cut(s)	516, 841, 874, 880
RseI	CAYNNNNRTG	1 cut(s)	554
SalI	GTCGAC	3 cut(s)	68, 269, 617
SaqAI	TTAA	3 cut(s)	234, 258, 948
Sau3AI	GATC	2 cut(s)	541, 624
Sau96I	GGNCC	1 cut(s)	220
ScaI	AGTACT	1 cut(s)	842
ScrFI	CCNGG	5 cut(s)	159, 211, 888, 938, 953
SduI	GDGCHC	4 cut(s)	434, 563, 936, 966
SfaNI	GCATC	5 cut(s)	110, 172, 471, 480, 872
SfoI	GGCGCC	2 cut(s)	652, 996
SgrAI	CRCCGGYG	1 cut(s)	631
SinI	GGWCC	1 cut(s)	220
SmiMI	CAYNNNNRTG	1 cut(s)	554
SmlI	CTYRAG	1 cut(s)	233
SmoI	CTYRAG	1 cut(s)	233
SspDI	GGCGCC	2 cut(s)	650, 994
StyD4I	CCNGG	5 cut(s)	157, 209, 886, 936, 951
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	6 cut(s)	88, 205, 436, 520, 553, 1028
TaiI	ACGT	1 cut(s)	624
TaqI	TCGA	4 cut(s)	69, 270, 618, 866
TaqII	GACCGA	1 cut(s)	116
TatI	WGTACW	2 cut(s)	515, 840
TauI	GCSGC	7 cut(s)	50, 230, 416, 696, 714, 863, 1000
TfiI	GAWTC	1 cut(s)	448
Tru1I	TTAA	3 cut(s)	234, 258, 948
Tru9I	TTAA	3 cut(s)	234, 258, 948
TscAI	CASTG	3 cut(s)	91, 439, 525
TseFI	GTSAC	3 cut(s)	205, 547, 716
TseI	GCWGC	5 cut(s)	416, 683, 755, 794, 797
Tsp45I	GTSAC	3 cut(s)	205, 547, 716
TspDTI	ATGAA	1 cut(s)	351
TspRI	CASTG	3 cut(s)	91, 439, 525
Tth111I	GACNNNGTC	1 cut(s)	275
Vha464I	CTTAAG	1 cut(s)	233
VpaK11BI	GGWCC	1 cut(s)	220
XceI	RCATGY	2 cut(s)	896, 947
XmiI	GTMKAC	3 cut(s)	69, 270, 618
ZrmI	AGTACT	1 cut(s)	842
Zsp2I	ATGCAT	1 cut(s)	111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.