Rh3BG181600

Axoneme-associated protein mst101(2)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0006291
Physical Location & Seq
Reverse (-)
15988721 .. 15991542
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG181600.1

Sequence Viewer

Length: 894 bp
ATGGTTTTAGGTGTTGAACAAGTGAAGACAGATGCCGGAGCCAACAAACTGACGGTGACCGGGAAAGTGGACGCCGCCGCTCTGAAGGCGAAACTCGAGGAGAGGATTAGGAAGAAGGTCGACCTCGTCTCTCCTCAGCCGGCCAAAAAGGAAGGAGGCGGTGAAGGAGGAGGAGACAAGAAGAAGGCGGAGGAAAAGTCCGAAAAGCCCTCGGAGAAGAAAGCCGAGGAAAAAAAGCCGGCCGATCAGCCCAAAGAGAAAGAGGCCCCCAAAGAGAGCACGATCGCTTTGAAGATCCGGTTGCATTGCGAGGGTTGCATCCAGAAGATGCGCAGTAAAATCTTGAAGTTCAAAGGTGTTAGTACGGTGGCCTTCGACATGGCCAAAGATCTGGTCACGGTGGTGGGCACGATGGACACCAAAGCGCTAGTTCCCTATCTGTCGGAGAAGTTCAAGAGACCGGTCGACGTGATCGCGCCGGCGAAGAAGGACGACGGCGCCGGAGCCAAGAAAGATGATAAGAAAGCAGCTGCCGGAGGCGGCGAGAAGAAAGACGGAGGTGGTGACAAGAAGGAAGAAAATAAAGAGGGAAAGAAGGAAGCTGCCCCCGGAGGCGGTGGAGGCGAGAAGAAAGAAGCTGCTGCTGCCGGCGGCGGTGGAGGTGCGATAGTGAACAAAATGGAGTATTCCGGCTACCCTTACCCGGCGTCGACGTCGTACTGGTACGACCCCGGACACGTTTACAACCACAACAAGTTTGTGATGGAGGCTCAGGAGCACCAGGCGCATGTTAGCCAAGGGTACGGGAACTACAACTACGCAATGCCCATGGAGCACTACCCTGAGCCGCAGATGTTCAGCGACGAGAACCCCAACGGTTGTTCGGTGATGTGA

Protein Analysis

297

Amino Acids

31.85

Weight (kDa)

8.84

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 99 - 144 2.7e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 716
Acc16I TGCGCA 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 3 cut(s) 120, 465, 710
AccII CGCG 1 cut(s) 476
AciI CCGC 9 cut(s) 75, 78, 159, 188, 540, 615, 651, 654, 848
AclWI GGATC 1 cut(s) 289
AcoI YGGCCR 3 cut(s) 141, 240, 381
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 4 cut(s) 72, 498, 707, 713
AfaI GTAC 4 cut(s) 364, 719, 725, 803
AfeI AGCGCT 1 cut(s) 426
AfiI CCNNNNNNNGG 2 cut(s) 614, 703
AflIII ACRYGT 1 cut(s) 736
AgeI ACCGGT 1 cut(s) 460
AgsI TTSAA 5 cut(s) 17, 292, 346, 352, 454
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 780
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 3 cut(s) 530, 602, 638
AluI AGCT 3 cut(s) 530, 602, 638
Alw21I GWGCWC 3 cut(s) 281, 780, 837
Alw26I GTCTC 3 cut(s) 133, 168, 451
AlwI GGATC 1 cut(s) 289
Ama87I CYCGRG 1 cut(s) 95
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 5 cut(s) 141, 240, 264, 369, 381
ApeKI GCWGC 6 cut(s) 527, 530, 602, 638, 641, 644
ArsI GACNNNNNNTTYG 2 cut(s) 378, 410
AsiGI ACCGGT 1 cut(s) 460
AspLEI GCGC 5 cut(s) 333, 427, 478, 500, 787
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 4 cut(s) 61, 609, 704, 732
AsuHPI GGTGA 3 cut(s) 67, 173, 575
AvaI CYCGRG 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 383
BanI GGYRCC 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 3 cut(s) 281, 780, 837
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 6 cut(s) 517, 539, 589, 625, 628, 631
BccI CCATC 2 cut(s) 406, 757
BceAI ACGGC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 782
BcnI CCSGG 4 cut(s) 61, 609, 704, 732
BcoDI GTCTC 3 cut(s) 133, 168, 451
BfaI CTAG 1 cut(s) 428
BfoI RGCGCY 2 cut(s) 428, 501
BglII AGATCT 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 61, 609, 704, 732, 782
BmeT110I CYCGRG 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 4 cut(s) 40, 267, 499, 505
BmrFI CCNGG 5 cut(s) 61, 609, 704, 732, 782
BmsI GCATC 3 cut(s) 22, 318, 327
BpiI GAAGAC 1 cut(s) 32
Bpu10I CCTNAGC 3 cut(s) 135, 771, 843
BpuMI CCSGG 4 cut(s) 61, 609, 704, 732
BsaHI GRCGYC 4 cut(s) 72, 498, 707, 713
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 6 cut(s) 210, 225, 607, 730, 796, 828
BsaWI WCCGGW 2 cut(s) 297, 460
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 614, 703
Bse118I RCCGGY 5 cut(s) 139, 238, 460, 478, 647
Bse1I ACTGG 1 cut(s) 725
Bse3DI GCAATG 2 cut(s) 304, 828
BseBI CCWGG 1 cut(s) 782
BseDI CCNNGG 6 cut(s) 210, 225, 607, 730, 796, 828
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 2 cut(s) 614, 703
BseMI GCAATG 2 cut(s) 304, 828
BseMII CTCAG 3 cut(s) 149, 785, 834
BseNI ACTGG 1 cut(s) 725
BseRI GAGGAG 4 cut(s) 113, 123, 183, 186
BseSI GKGCMC 1 cut(s) 410
BseX3I CGGCCG 1 cut(s) 240
BseXI GCAGC 6 cut(s) 517, 539, 589, 625, 628, 631
Bsh1236I CGCG 1 cut(s) 476
Bsh1285I CGRYCG 3 cut(s) 243, 285, 465
BshFI GGCC 5 cut(s) 143, 242, 266, 371, 383
BshNI GGYRCC 1 cut(s) 497
BshTI ACCGGT 1 cut(s) 460
BsiEI CGRYCG 3 cut(s) 243, 285, 465
BsiHKAI GWGCWC 3 cut(s) 281, 780, 837
BsiHKCI CYCGRG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 614, 703
BsmAI GTCTC 3 cut(s) 133, 168, 451
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 5 cut(s) 143, 242, 266, 371, 383
Bso31I GGTCTC 1 cut(s) 451
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 4 cut(s) 281, 410, 780, 837
Bsp143I GATC 5 cut(s) 244, 282, 294, 388, 471
Bsp19I CCATGG 1 cut(s) 828
BspACI CCGC 9 cut(s) 75, 78, 159, 188, 540, 615, 651, 654, 848
BspANI GGCC 5 cut(s) 143, 242, 266, 371, 383
BspCNI CTCAG 3 cut(s) 148, 784, 835
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 4 cut(s) 40, 267, 499, 505
BspPI GGATC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 304, 828
BsrFI RCCGGY 5 cut(s) 139, 238, 460, 478, 647
BsrI ACTGG 1 cut(s) 725
BssAI RCCGGY 5 cut(s) 139, 238, 460, 478, 647
BssECI CCNNGG 6 cut(s) 210, 225, 607, 730, 796, 828
BssMI GATC 5 cut(s) 244, 282, 294, 388, 471
BssNI GRCGYC 4 cut(s) 72, 498, 707, 713
BssT1I CCWWGG 2 cut(s) 796, 828
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 4 cut(s) 55, 367, 400, 878
BstACI GRCGYC 4 cut(s) 72, 498, 707, 713
BstC8I GCNNGC 4 cut(s) 141, 240, 480, 649
BstDEI CTNAG 3 cut(s) 135, 771, 843
BstDSI CCRYGG 1 cut(s) 828
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 2 cut(s) 428, 501
BstHHI GCGC 5 cut(s) 333, 427, 478, 500, 787
BstKTI GATC 5 cut(s) 247, 285, 297, 391, 474
BstMAI GTCTC 3 cut(s) 133, 168, 451
BstMBI GATC 5 cut(s) 244, 282, 294, 388, 471
BstMCI CGRYCG 3 cut(s) 243, 285, 465
BstMWI GCNNNNNNNGC 6 cut(s) 86, 315, 621, 644, 784, 832
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 1 cut(s) 791
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 5 cut(s) 59, 607, 702, 730, 780
BstSLI GKGCMC 1 cut(s) 410
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 6 cut(s) 517, 539, 589, 625, 628, 631
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 2 cut(s) 294, 388
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 2 cut(s) 294, 388
BstZI CGGCCG 1 cut(s) 240
BsuRI GGCC 5 cut(s) 143, 242, 266, 371, 383
BtgI CCRYGG 1 cut(s) 828
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 1 cut(s) 318
Cac8I GCNNGC 4 cut(s) 141, 240, 480, 649
CfoI GCGC 5 cut(s) 333, 427, 478, 500, 787
Cfr10I RCCGGY 5 cut(s) 139, 238, 460, 478, 647
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 2 cut(s) 80, 696
Csp6I GTAC 4 cut(s) 363, 718, 724, 802
CspAI ACCGGT 1 cut(s) 460
CviAII CATG 3 cut(s) 379, 788, 829
CviQI GTAC 4 cut(s) 363, 718, 724, 802
DdeI CTNAG 3 cut(s) 135, 771, 843
DinI GGCGCC 1 cut(s) 499
DpnI GATC 5 cut(s) 246, 284, 296, 390, 473
DpnII GATC 5 cut(s) 244, 282, 294, 388, 471
EaeI YGGCCR 3 cut(s) 141, 240, 381
EagI CGGCCG 1 cut(s) 240
EciI GGCGGA 1 cut(s) 203
EclXI CGGCCG 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 796, 828
Eco31I GGTCTC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 426
Eco52I CGGCCG 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 95
Eco91I GGTNACC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 780
EcoT14I CCWWGG 2 cut(s) 796, 828
EgeI GGCGCC 1 cut(s) 499
EheI GGCGCC 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 796, 828
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 3 cut(s) 382, 791, 832
FaiI YATR 3 cut(s) 380, 789, 830
FatI CATG 3 cut(s) 378, 787, 828
FblI GTMKAC 3 cut(s) 120, 465, 710
FokI GGATG 1 cut(s) 305
FspBI CTAG 1 cut(s) 428
FspI TGCGCA 1 cut(s) 332
GlaI GCGC 5 cut(s) 332, 426, 477, 499, 786
HaeII RGCGCY 2 cut(s) 428, 501
HaeIII GGCC 5 cut(s) 143, 242, 266, 371, 383
HgaI GACGC 2 cut(s) 80, 696
HhaI GCGC 5 cut(s) 333, 427, 478, 500, 787
Hin1I GRCGYC 4 cut(s) 72, 498, 707, 713
Hin1II CATG 3 cut(s) 382, 791, 832
Hin6I GCGC 5 cut(s) 331, 425, 476, 498, 785
HinP1I GCGC 5 cut(s) 331, 425, 476, 498, 785
HincII GTYRAC 3 cut(s) 121, 466, 711
HindII GTYRAC 3 cut(s) 121, 466, 711
HphI GGTGA 3 cut(s) 67, 173, 575
Hpy166II GTNNAC 6 cut(s) 70, 121, 466, 673, 711, 742
Hpy188I TCNGA 4 cut(s) 84, 202, 214, 445
Hpy188III TCNNGA 4 cut(s) 322, 343, 454, 773
Hpy8I GTNNAC 6 cut(s) 70, 121, 466, 673, 711, 742
Hpy99I CGWCG 6 cut(s) 470, 497, 712, 715, 718, 866
HpyAV CCTTC 9 cut(s) 79, 109, 146, 158, 178, 382, 481, 565, 589
HpyCH4III ACNGT 4 cut(s) 55, 367, 400, 878
HpyCH4IV ACGT 3 cut(s) 468, 713, 738
HpyCH4V TGCA 2 cut(s) 304, 318
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 315, 621, 644, 784, 832
HpyF3I CTNAG 3 cut(s) 135, 771, 843
HpySE526I ACGT 3 cut(s) 468, 713, 738
Hsp92I GRCGYC 4 cut(s) 72, 498, 707, 713
Hsp92II CATG 3 cut(s) 382, 791, 832
HspAI GCGC 5 cut(s) 331, 425, 476, 498, 785
KasI GGCGCC 1 cut(s) 497
KroI GCCGGC 4 cut(s) 139, 238, 478, 647
KroNI GCCGGC 4 cut(s) 141, 240, 480, 649
Kzo9I GATC 5 cut(s) 244, 282, 294, 388, 471
LmnI GCTCC 4 cut(s) 38, 503, 775, 832
Lsp1109I GCAGC 6 cut(s) 517, 539, 589, 625, 628, 631
LweI GCATC 3 cut(s) 22, 318, 327
MaeI CTAG 1 cut(s) 428
MaeII ACGT 3 cut(s) 468, 713, 738
MaeIII GTNAC 3 cut(s) 55, 394, 563
MalI GATC 5 cut(s) 246, 284, 296, 390, 473
MbiI CCGCTC 1 cut(s) 80
MboI GATC 5 cut(s) 244, 282, 294, 388, 471
MflI RGATCY 2 cut(s) 294, 388
MhlI GDGCHC 4 cut(s) 281, 410, 780, 837
MlsI TGGCCA 1 cut(s) 383
MluNI TGGCCA 1 cut(s) 383
Mly113I GGCGCC 1 cut(s) 498
MmeI TCCRAC 1 cut(s) 423
Mox20I TGGCCA 1 cut(s) 383
MreI CGCCGGCG 1 cut(s) 478
MroNI GCCGGC 4 cut(s) 139, 238, 478, 647
MscI TGGCCA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 530
MspR9I CCNGG 5 cut(s) 61, 609, 704, 732, 782
MvaI CCWGG 1 cut(s) 782
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 6 cut(s) 86, 315, 621, 644, 784, 832
NaeI GCCGGC 4 cut(s) 141, 240, 480, 649
NarI GGCGCC 1 cut(s) 498
NciI CCSGG 4 cut(s) 61, 609, 704, 732
NcoI CCATGG 1 cut(s) 828
NdeII GATC 5 cut(s) 244, 282, 294, 388, 471
NgoMIV GCCGGC 4 cut(s) 139, 238, 478, 647
NlaIII CATG 3 cut(s) 382, 791, 832
NlaIV GGNNCC 4 cut(s) 40, 267, 499, 505
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 3 cut(s) 55, 394, 563
NsbI TGCGCA 1 cut(s) 332
NspI RCATGY 1 cut(s) 791
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 95
PdiI GCCGGC 4 cut(s) 141, 240, 480, 649
PflFI GACNNNGTC 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 460
Ple19I CGATCG 1 cut(s) 285
PluTI GGCGCC 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 780
PspEI GGTNACC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 4 cut(s) 40, 267, 499, 505
PspPI GGNCC 1 cut(s) 265
PspXI VCTCGAGB 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 800, 832
PsuI RGATCY 2 cut(s) 294, 388
PsyI GACNNNGTC 1 cut(s) 125
PvuI CGATCG 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 4 cut(s) 364, 719, 725, 803
RsaNI GTAC 4 cut(s) 363, 718, 724, 802
RseI CAYNNNNRTG 1 cut(s) 401
SalI GTCGAC 3 cut(s) 119, 464, 709
Sau3AI GATC 5 cut(s) 244, 282, 294, 388, 471
Sau96I GGNCC 1 cut(s) 265
ScrFI CCNGG 5 cut(s) 61, 609, 704, 732, 782
SduI GDGCHC 4 cut(s) 281, 410, 780, 837
SfaNI GCATC 3 cut(s) 22, 318, 327
SfoI GGCGCC 1 cut(s) 499
Sfr274I CTCGAG 1 cut(s) 95
SgrAI CRCCGGYG 1 cut(s) 478
SgrDI CGTCGACG 1 cut(s) 709
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 9 cut(s) 75, 78, 159, 188, 540, 615, 651, 654, 848
SspDI GGCGCC 1 cut(s) 497
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 5 cut(s) 59, 607, 702, 730, 780
StyI CCWWGG 2 cut(s) 796, 828
TaaI ACNGT 4 cut(s) 55, 367, 400, 878
TaiI ACGT 3 cut(s) 471, 716, 741
TaqI TCGA 5 cut(s) 96, 120, 375, 465, 710
TauI GCSGC 5 cut(s) 77, 80, 543, 654, 850
TseFI GTSAC 3 cut(s) 55, 394, 563
TseI GCWGC 6 cut(s) 527, 530, 602, 638, 641, 644
Tsp45I GTSAC 3 cut(s) 55, 394, 563
TspGWI ACGGA 1 cut(s) 570
Tth111I GACNNNGTC 1 cut(s) 125
XceI RCATGY 1 cut(s) 791
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 3 cut(s) 120, 465, 710
XspI CTAG 1 cut(s) 428
ZraI GACGTC 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.