FvH4_6g18590

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004631
Physical Location & Seq
Reverse (-)
12282058 .. 12284464
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18590.t1

Sequence Viewer

Length: 414 bp
ATGGCTTCGCAGATTCTGGCGGCGGCGACAGGGAGGACGGCGATGGCGGCGAGGAGGCAAGCGGTGAACTTGACGGAGGCGGCGGCTTCTAGAATACGGCAGCTGCTGGAGCAGCGGCAGCGGTCGTATCTGAAGCTCGGGGTTAAGGCGCGTGGCTGCAACGGCTTGTCGTATACTCTCAATTACGCCGATGATAAAGGAAAGTTTGACGAGTTGGTTGAAGATAAAGGCGTCAAGATATTGATCGATCCAAAGGCTCTGATGCATGTGATTGGCACCAAGATGGATTTTGTGGATGACAAGCTCAGGTCTGAGTTTATATTTATCAATCCCAACTCAAAAGGACAATGTGGCTGTGGTGAATCTTTCATGACAACCGGTAGTACTGGAGCTACTAAGAACAGCAACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

138

Amino Acids

14.89

Weight (kDa)

9.4

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 22 - 121 3.1e-19 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 1 cut(s) 173
AccII CGCG 1 cut(s) 151
AciI CCGC 8 cut(s) 20, 23, 47, 62, 80, 83, 115, 121
AclWI GGATC 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 385
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 1 cut(s) 221
AloI GAACNNNNNNTCC 1 cut(s) 392
AluBI AGCT 4 cut(s) 103, 136, 304, 392
AluI AGCT 4 cut(s) 103, 136, 304, 392
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 2 cut(s) 16, 106
Ama87I CYCGRG 1 cut(s) 137
ApeKI GCWGC 5 cut(s) 100, 103, 112, 118, 156
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 151
AsuHPI GGTGA 2 cut(s) 76, 371
AvaI CYCGRG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 90, 112, 124, 130, 143
BccI CCATC 2 cut(s) 37, 277
BceAI ACGGC 3 cut(s) 54, 113, 178
BfaI CTAG 1 cut(s) 90
BmcAI AGTACT 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 277
BmsI GCATC 1 cut(s) 252
BpmI CTGGAG 2 cut(s) 128, 408
Bpu10I CCTNAGC 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 1 cut(s) 392
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 391
Bse8I GATNNNNATC 1 cut(s) 242
BseCI ATCGAT 1 cut(s) 246
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 1 cut(s) 242
BseMII CTCAG 2 cut(s) 303, 319
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 1 cut(s) 67
BseXI GCAGC 5 cut(s) 90, 112, 124, 130, 143
Bsh1236I CGCG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 275
BshTI ACCGGT 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 378
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 2 cut(s) 243, 247
BspACI CCGC 8 cut(s) 20, 23, 47, 62, 80, 83, 115, 121
BspCNI CTCAG 2 cut(s) 304, 318
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 1 cut(s) 151
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 377
BssMI GATC 2 cut(s) 243, 247
BssNAI GTATAC 1 cut(s) 174
BssNI GRCGYC 1 cut(s) 231
Bst1107I GTATAC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 305, 312, 396
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 2 cut(s) 246, 250
BstMBI GATC 2 cut(s) 243, 247
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 5 cut(s) 47, 109, 112, 118, 162
BstNSI RCATGY 1 cut(s) 269
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 5 cut(s) 90, 112, 124, 130, 143
BstZ17I GTATAC 1 cut(s) 174
Bsu15I ATCGAT 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 246
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 60
CaiI CAGNNNCTG 2 cut(s) 16, 106
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 377
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 384
CspAI ACCGGT 1 cut(s) 377
CviAII CATG 2 cut(s) 266, 370
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 3 cut(s) 305, 312, 396
DpnI GATC 2 cut(s) 245, 249
DpnII GATC 2 cut(s) 243, 247
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 267
FaeI CATG 2 cut(s) 269, 373
FaiI YATR 4 cut(s) 174, 267, 320, 371
FatI CATG 2 cut(s) 265, 369
FblI GTMKAC 1 cut(s) 173
FokI GGATG 1 cut(s) 308
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 150
GsuI CTGGAG 2 cut(s) 128, 408
HapII CCGG 1 cut(s) 378
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 269, 373
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 2 cut(s) 13, 362
HpaII CCGG 1 cut(s) 378
HphI GGTGA 2 cut(s) 76, 371
Hpy166II GTNNAC 2 cut(s) 67, 174
Hpy188I TCNGA 3 cut(s) 132, 261, 313
Hpy188III TCNNGA 3 cut(s) 90, 235, 370
Hpy8I GTNNAC 2 cut(s) 67, 174
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 109, 112, 118, 162
HpyF3I CTNAG 3 cut(s) 305, 312, 396
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 269, 373
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 2 cut(s) 243, 247
LmnI GCTCC 2 cut(s) 109, 389
LpnPI CCDG 6 cut(s) 2, 15, 92, 292, 372, 391
Lsp1109I GCAGC 5 cut(s) 90, 112, 124, 130, 143
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 90
MalI GATC 2 cut(s) 245, 249
MboI GATC 2 cut(s) 243, 247
MboII GAAGA 1 cut(s) 233
MluCI AATT 1 cut(s) 181
MnlI CCTC 4 cut(s) 27, 45, 48, 70
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 3 cut(s) 103, 115, 121
MspI CCGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 5 cut(s) 47, 109, 112, 118, 162
NdeII GATC 2 cut(s) 243, 247
NlaIII CATG 2 cut(s) 269, 373
NlaIV GGNNCC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PagI TCATGA 1 cut(s) 369
PfeI GAWTC 2 cut(s) 13, 362
PinAI ACCGGT 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 277
PstNI CAGNNNCTG 2 cut(s) 16, 106
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 144
Sau3AI GATC 2 cut(s) 243, 247
ScaI AGTACT 1 cut(s) 385
SetI ASST 5 cut(s) 105, 138, 306, 311, 394
SfaNI GCATC 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 1 cut(s) 181
SsiI CCGC 8 cut(s) 20, 23, 47, 62, 80, 83, 115, 121
SspMI CTAG 1 cut(s) 90
TaqI TCGA 1 cut(s) 246
TasI AATT 1 cut(s) 181
TatI WGTACW 1 cut(s) 383
TauI GCSGC 6 cut(s) 23, 26, 50, 83, 86, 118
TfiI GAWTC 2 cut(s) 13, 362
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 5 cut(s) 100, 103, 112, 118, 156
TspDTI ATGAA 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 89
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 173
XspI CTAG 1 cut(s) 90
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.