Rw0G019030

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00856
OG0004631
Physical Location & Seq
Reverse (-)
18853 .. 20440
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019030.1

Sequence Viewer

Length: 366 bp
ATGGCTTCGCAGATTCTGGCTGCGGCGGCGGGGAGGACGGCAGCGGCGGCGAGGCGGCAAGCGCAGCGCCAGCGGTCGTATCTGAAGCTCGGGGTTAAGGCGCGTGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGATGATAAGGGAAAGTTCGATGAGTTGGTTGAAGATAAAGGCGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGAACCAAGATGGATTTTGTGGATGACAAACTCAGGTCTGAGTTTACATTTATCAATCCCAACTCAAAAGGACAATGCGGCTGTGGTGAATCTTTTATGACAACAGGTAGTACTGGAGCTACTAAGCAAAGCAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

121

Amino Acids

13.02

Weight (kDa)

9.4

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 14 - 105 2.9e-15 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 103
AciI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 303
AclWI GGATC 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 2 cut(s) 125, 337
AgsI TTSAA 1 cut(s) 173
AluBI AGCT 2 cut(s) 88, 344
AluI AGCT 2 cut(s) 88, 344
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 89
ApeKI GCWGC 5 cut(s) 20, 41, 64, 108, 357
AspLEI GCGC 3 cut(s) 64, 69, 103
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 89
BbvI GCAGC 4 cut(s) 7, 53, 76, 95
BccI CCATC 1 cut(s) 229
BceAI ACGGC 2 cut(s) 54, 130
BfoI RGCGCY 1 cut(s) 70
BmcAI AGTACT 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 229
BmsI GCATC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 360
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 198
BseGI GGATG 1 cut(s) 253
BseJI GATNNNNATC 1 cut(s) 194
BseMII CTCAG 2 cut(s) 255, 271
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 4 cut(s) 7, 53, 76, 95
Bsh1236I CGCG 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 77
BsiHKCI CYCGRG 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 89
Bsp143I GATC 2 cut(s) 195, 199
BspACI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 303
BspCNI CTCAG 2 cut(s) 256, 270
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 229
BspPI GGATC 1 cut(s) 194
BsrI ACTGG 1 cut(s) 343
BssMI GATC 2 cut(s) 195, 199
BstC8I GCNNGC 2 cut(s) 60, 71
BstDEI CTNAG 3 cut(s) 257, 264, 348
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 103
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 3 cut(s) 64, 69, 103
BstKTI GATC 2 cut(s) 198, 202
BstMBI GATC 2 cut(s) 195, 199
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
BstNSI RCATGY 1 cut(s) 221
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 4 cut(s) 7, 53, 76, 95
Bsu15I ATCGAT 1 cut(s) 198
BsuTUI ATCGAT 1 cut(s) 198
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 2 cut(s) 60, 71
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 3 cut(s) 64, 69, 103
ClaI ATCGAT 1 cut(s) 198
Csp6I GTAC 2 cut(s) 124, 336
CviAII CATG 1 cut(s) 218
CviJI RGCY 9 cut(s) 5, 20, 88, 108, 117, 209, 306, 344, 360
CviKI_1 RGCY 9 cut(s) 5, 20, 88, 108, 117, 209, 306, 344, 360
CviQI GTAC 2 cut(s) 124, 336
DdeI CTNAG 3 cut(s) 257, 264, 348
DpnI GATC 2 cut(s) 197, 201
DpnII GATC 2 cut(s) 195, 199
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 219
FaeI CATG 1 cut(s) 221
FaiI YATR 2 cut(s) 219, 323
FatI CATG 1 cut(s) 217
FauI CCCGC 1 cut(s) 22
FokI GGATG 1 cut(s) 260
GlaI GCGC 3 cut(s) 63, 68, 102
GsuI CTGGAG 1 cut(s) 360
HaeII RGCGCY 1 cut(s) 70
HhaI GCGC 3 cut(s) 64, 69, 103
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 3 cut(s) 62, 67, 101
HinP1I GCGC 3 cut(s) 62, 67, 101
HinfI GANTC 2 cut(s) 13, 314
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 126, 270
Hpy188I TCNGA 3 cut(s) 84, 213, 265
Hpy8I GTNNAC 2 cut(s) 126, 270
HpyCH4V TGCA 2 cut(s) 111, 217
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
HpyF3I CTNAG 3 cut(s) 257, 264, 348
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 3 cut(s) 62, 67, 101
Kzo9I GATC 2 cut(s) 195, 199
LmnI GCTCC 1 cut(s) 341
LpnPI CCDG 5 cut(s) 2, 83, 244, 315, 324
Lsp1109I GCAGC 4 cut(s) 7, 53, 76, 95
LweI GCATC 1 cut(s) 204
MalI GATC 2 cut(s) 197, 201
MboI GATC 2 cut(s) 195, 199
MboII GAAGA 2 cut(s) 185, 199
MluCI AATT 1 cut(s) 133
MnlI CCTC 2 cut(s) 27, 45
Mph1103I ATGCAT 1 cut(s) 219
MseI TTAA 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 44, 73
MteI GCGCNGCGC 1 cut(s) 65
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
NdeII GATC 2 cut(s) 195, 199
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 219
NspI RCATGY 1 cut(s) 221
PfeI GAWTC 2 cut(s) 13, 314
PspN4I GGNNCC 1 cut(s) 229
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 125, 337
RsaNI GTAC 2 cut(s) 124, 336
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 2 cut(s) 195, 199
ScaI AGTACT 1 cut(s) 337
SetI ASST 4 cut(s) 90, 263, 334, 346
SfaNI GCATC 1 cut(s) 204
Sse9I AATT 1 cut(s) 133
SsiI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 303
TaqI TCGA 2 cut(s) 159, 198
TasI AATT 1 cut(s) 133
TatI WGTACW 1 cut(s) 335
TauI GCSGC 6 cut(s) 26, 29, 47, 50, 58, 306
TfiI GAWTC 2 cut(s) 13, 314
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseI GCWGC 5 cut(s) 20, 41, 64, 108, 357
XceI RCATGY 1 cut(s) 221
ZrmI AGTACT 1 cut(s) 337
Zsp2I ATGCAT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.