Rorug06G0055200

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004631
Physical Location & Seq
Reverse (-)
7588585 .. 7589686
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0055200.1

Sequence Viewer

Length: 645 bp
ATGAGCTGCAATGGATGCCGAGTTTTGCGAAAGGGTTGCAGCGAGAATTGCATGCTACGTCAATGCATAGAGTGGATGGAAGACCCTCAAGCTCAAGCTCACGCCACCGTTTTCGTCGCCAAGTTCTTCGGCCGCGCCGGCCTCCTCTCCTTCATCAACGCTGTCTCCGAAAGCCAAAGACCAGCTTTGTTTCAGTCTTTGTTGTTTGAGGCGGTGGGGAGGACTATAAATCCGGTGAACGGAGCGACGGGGCTTCTTTTGGCTGGGAACTGGAAGGTCTGCCAGGCTGCGGTGGAGAAGGTGCTGAGCGGCGGCGCGTTGGAGCCACTTCCCGAGTACAGTCGTCGTGGCATTCATCACGTTCGACAACAACGGCGGGATGAGCGCTATTCCTCCAAGTACTCGACCAAAAAGAGGCCGGCTTTGTTGGCTTTTGACGACGACGACGACGATGATGAACGGCCTGATCTTGATCTTAGCTTGAAGGCATCGTCGGAGTTGCATAGAGATGATCACGATAGAGCGGCGACGCCGTCGTCTGTGGAAGGCTCGGAAACGACGACGCTGTCAAGAAGTGGTTCGTCGTCGGCAGATTGTACTACTAATGATTCAGAGGGAACAAGGCTTCTAAGACTATTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

214

Amino Acids

23.62

Weight (kDa)

5.98

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 99 2.6e-23 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 535, 565
AccBSI CCGCTC 2 cut(s) 309, 524
AccII CGCG 2 cut(s) 135, 317
AciI CCGC 7 cut(s) 133, 212, 290, 309, 312, 376, 524
AcoI YGGCCR 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 3 cut(s) 338, 401, 598
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 3 cut(s) 239, 289, 414
AgsI TTSAA 1 cut(s) 484
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 5 cut(s) 6, 92, 98, 185, 480
AluI AGCT 5 cut(s) 6, 92, 98, 185, 480
Alw26I GTCTC 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 4 cut(s) 130, 139, 416, 461
ApeKI GCWGC 3 cut(s) 6, 39, 287
ArsI GACNNNNNNTTYG 1 cut(s) 624
Asp700I GAANNNNTTC 1 cut(s) 577
AspLEI GCGC 3 cut(s) 137, 317, 387
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 51, 274
BccI CCATC 1 cut(s) 70
BceAI ACGGC 3 cut(s) 389, 476, 517
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 388
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 525
BlpI GCTNAGC 1 cut(s) 305
BlsI GCNGC 7 cut(s) 8, 41, 134, 289, 311, 314, 526
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 2 cut(s) 5, 497
BpiI GAAGAC 1 cut(s) 87
Bpu1102I GCTNAGC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 72, 78
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 530
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 289, 414
Bse118I RCCGGY 2 cut(s) 137, 418
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 284
BseGI GGATG 3 cut(s) 20, 81, 385
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 3 cut(s) 239, 289, 414
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 134
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 51, 274
BseYI CCCAGC 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 135, 317
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 4 cut(s) 132, 141, 418, 463
BsiEI CGRYCG 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 3 cut(s) 138, 233, 419
BslI CCNNNNNNNGG 3 cut(s) 239, 289, 414
BsmAI GTCTC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 4 cut(s) 132, 141, 418, 463
BsoBI CYCGRG 1 cut(s) 332
Bsp143I GATC 3 cut(s) 466, 472, 511
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 7 cut(s) 133, 212, 290, 309, 312, 376, 524
BspANI GGCC 4 cut(s) 132, 141, 418, 463
BspCNI CTCAG 1 cut(s) 297
BspFNI CGCG 2 cut(s) 135, 317
BspLI GGNNCC 1 cut(s) 324
BsrBI CCGCTC 2 cut(s) 309, 524
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 2 cut(s) 137, 418
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 2 cut(s) 137, 418
BssMI GATC 3 cut(s) 466, 472, 511
BssNI GRCGYC 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 109, 341
BstACI GRCGYC 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 53, 139, 420
BstDEI CTNAG 3 cut(s) 305, 476, 629
BstF5I GGATG 3 cut(s) 20, 81, 385
BstFNI CGCG 2 cut(s) 135, 317
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 3 cut(s) 137, 317, 387
BstKTI GATC 3 cut(s) 469, 475, 514
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 3 cut(s) 466, 472, 511
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 382, 428
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 282
BstUI CGCG 2 cut(s) 135, 317
BstV1I GCAGC 2 cut(s) 51, 274
BstV2I GAAGAC 1 cut(s) 87
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 4 cut(s) 132, 141, 418, 463
BtsCI GGATG 3 cut(s) 20, 81, 385
Cac8I GCNNGC 3 cut(s) 53, 139, 420
CfoI GCGC 3 cut(s) 137, 317, 387
Cfr10I RCCGGY 2 cut(s) 137, 418
CseI GACGC 2 cut(s) 538, 571
Csp6I GTAC 3 cut(s) 337, 400, 597
CviAII CATG 1 cut(s) 52
CviQI GTAC 3 cut(s) 337, 400, 597
DdeI CTNAG 3 cut(s) 305, 476, 629
DpnI GATC 3 cut(s) 468, 474, 513
DpnII GATC 3 cut(s) 466, 472, 511
DrdI GACNNNNNNGTC 2 cut(s) 535, 565
DseDI GACNNNNNNGTC 2 cut(s) 535, 565
EaeI YGGCCR 1 cut(s) 130
EagI CGGCCG 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 130
Eco47III AGCGCT 1 cut(s) 386
Eco52I CGGCCG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 68
FaeI CATG 1 cut(s) 55
FaiI YATR 4 cut(s) 53, 68, 227, 504
FalI AAGNNNNNCTT 2 cut(s) 169, 201
FatI CATG 1 cut(s) 51
FauI CCCGC 1 cut(s) 369
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 525
FokI GGATG 3 cut(s) 27, 88, 392
Fsp4HI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 525
GlaI GCGC 3 cut(s) 136, 316, 386
GluI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 525
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 4 cut(s) 132, 141, 418, 463
HapII CCGG 3 cut(s) 138, 233, 419
HgaI GACGC 2 cut(s) 538, 571
HhaI GCGC 3 cut(s) 137, 317, 387
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 3 cut(s) 135, 315, 385
HinP1I GCGC 3 cut(s) 135, 315, 385
HinfI GANTC 1 cut(s) 608
HpaII CCGG 3 cut(s) 138, 233, 419
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 4 cut(s) 169, 496, 553, 613
Hpy188III TCNNGA 4 cut(s) 332, 470, 515, 570
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 5 cut(s) 160, 268, 292, 478, 539
HpyCH4III ACNGT 2 cut(s) 109, 341
HpyCH4IV ACGT 2 cut(s) 58, 360
HpyCH4V TGCA 5 cut(s) 9, 39, 51, 66, 502
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 382, 428
HpyF3I CTNAG 3 cut(s) 305, 476, 629
HpySE526I ACGT 2 cut(s) 58, 360
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 3 cut(s) 135, 315, 385
KroI GCCGGC 2 cut(s) 137, 418
KroNI GCCGGC 2 cut(s) 139, 420
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 3 cut(s) 466, 472, 511
LmnI GCTCC 2 cut(s) 242, 322
LpnPI CCDG 9 cut(s) 151, 195, 246, 249, 256, 269, 296, 432, 477
Lsp1109I GCAGC 2 cut(s) 51, 274
LweI GCATC 2 cut(s) 5, 497
MaeII ACGT 2 cut(s) 58, 360
MalI GATC 3 cut(s) 468, 474, 513
MbiI CCGCTC 2 cut(s) 309, 524
MboI GATC 3 cut(s) 466, 472, 511
MboII GAAGA 2 cut(s) 92, 118
MluCI AATT 1 cut(s) 46
MmeI TCCRAC 2 cut(s) 300, 474
MnlI CCTC 8 cut(s) 96, 152, 155, 202, 213, 403, 408, 607
Mph1103I ATGCAT 1 cut(s) 68
MroNI GCCGGC 2 cut(s) 137, 418
MroXI GAANNNNTTC 1 cut(s) 577
MslI CAYNNNNRTG 1 cut(s) 507
MspI CCGG 3 cut(s) 138, 233, 419
MspR9I CCNGG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 2 cut(s) 135, 317
MwoI GCNNNNNNNGC 5 cut(s) 15, 48, 138, 382, 428
NaeI GCCGGC 2 cut(s) 139, 420
NdeII GATC 3 cut(s) 466, 472, 511
NgoMIV GCCGGC 2 cut(s) 137, 418
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 324
NmeAIII GCCGAG 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 68
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 444, 447, 557
PctI GAATGC 1 cut(s) 351
PdiI GCCGGC 2 cut(s) 139, 420
PdmI GAANNNNTTC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 608
PflFI GACNNNGTC 2 cut(s) 532, 565
PkrI GCNGC 7 cut(s) 8, 41, 134, 289, 311, 314, 526
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 324
PsyI GACNNNGTC 2 cut(s) 532, 565
RsaI GTAC 3 cut(s) 338, 401, 598
RsaNI GTAC 3 cut(s) 337, 400, 597
RseI CAYNNNNRTG 1 cut(s) 507
SatI GCNGC 7 cut(s) 7, 40, 133, 288, 310, 313, 525
Sau3AI GATC 3 cut(s) 466, 472, 511
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 284
SetI ASST 9 cut(s) 8, 61, 94, 100, 187, 279, 303, 363, 482
SfaNI GCATC 2 cut(s) 5, 497
SfiI GGCCNNNNNGGCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 87, 93
SmoI CTYRAG 2 cut(s) 87, 93
SphI GCATGC 1 cut(s) 55
Sse9I AATT 1 cut(s) 46
SsiI CCGC 7 cut(s) 133, 212, 290, 309, 312, 376, 524
StyD4I CCNGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 109, 341
TaiI ACGT 2 cut(s) 61, 363
TaqI TCGA 2 cut(s) 364, 404
TasI AATT 1 cut(s) 46
TatI WGTACW 3 cut(s) 336, 399, 596
TauI GCSGC 4 cut(s) 135, 312, 315, 527
TfiI GAWTC 1 cut(s) 608
TseI GCWGC 3 cut(s) 6, 39, 287
TspDTI ATGAA 4 cut(s) 142, 344, 471, 628
TspGWI ACGGA 1 cut(s) 255
Tth111I GACNNNGTC 2 cut(s) 532, 565
XceI RCATGY 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 577
ZrmI AGTACT 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.