Rw6G014860

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004631
Physical Location & Seq
Forward (+)
27801228 .. 27802796
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014860.1

Sequence Viewer

Length: 414 bp
ATGGCATCCCAGTTTCTGGCAGCGGCGGCGGAGAGGATAGCTCCGGCGGCGAGGCGCCAAGCCGTCATCTTAACAGACGCTGCGGCATCTAGAGTACGACAGCTCCTAGAGCAGCGCCAGCGGTCGTATCTCAAGCTCGGCGTCAAGTCCCGAGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGATGATAAAGGTAAGTTCGACGAGTTGATCGAAGACAAGGGTGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGGACGAAGATGGATTTTGTAGATGATAAGCTCAGGTCCGAGTTTGTCTTTATCAATCCCAACTCCAAAGGACAATGTGGTTGTGGTGAATCTTTCATGACAACAAGCAGTAGTGGAGCTACTAAACAGAGTGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

137

Amino Acids

14.89

Weight (kDa)

9.24

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 23 - 121 1e-18 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 16
AciI CCGC 6 cut(s) 23, 26, 29, 47, 83, 121
AclWI GGATC 1 cut(s) 242
AcyI GRCGYC 2 cut(s) 55, 141
AfaI GTAC 2 cut(s) 96, 173
AfiI CCNNNNNNNGG 1 cut(s) 16
AluBI AGCT 5 cut(s) 41, 103, 136, 304, 392
AluI AGCT 5 cut(s) 41, 103, 136, 304, 392
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 2 cut(s) 16, 80
Ama87I CYCGRG 1 cut(s) 150
ApeKI GCWGC 4 cut(s) 20, 80, 112, 156
AspLEI GCGC 2 cut(s) 57, 117
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 309
BanI GGYRCC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 4 cut(s) 32, 67, 124, 143
BccI CCATC 1 cut(s) 277
BceAI ACGGC 2 cut(s) 47, 178
BfaI CTAG 2 cut(s) 90, 107
BfoI RGCGCY 2 cut(s) 58, 118
BisI GCNGC 8 cut(s) 21, 24, 27, 48, 81, 84, 113, 157
BlsI GCNGC 8 cut(s) 22, 25, 28, 49, 82, 85, 114, 158
Bme18I GGWCC 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 56
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 3 cut(s) 14, 95, 252
BmuI ACTGGG 1 cut(s) 4
BpiI GAAGAC 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 25, 57
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 2 cut(s) 55, 141
BsaJI CCNNGG 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 10
Bse8I GATNNNNATC 1 cut(s) 242
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 5
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 10
BseXI GCAGC 4 cut(s) 32, 67, 124, 143
Bsh1285I CGRYCG 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 133, 289
BslI CCNNNNNNNGG 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 133, 289
BsoBI CYCGRG 1 cut(s) 150
Bsp143I GATC 3 cut(s) 216, 243, 247
BspACI CCGC 6 cut(s) 23, 26, 29, 47, 83, 121
BspCNI CTCAG 1 cut(s) 318
BspDI ATCGAT 1 cut(s) 246
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 56
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 54
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 3 cut(s) 216, 243, 247
BssNI GRCGYC 2 cut(s) 55, 141
BstACI GRCGYC 2 cut(s) 55, 141
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 5
BstH2I RGCGCY 2 cut(s) 58, 118
BstHHI GCGC 2 cut(s) 57, 117
BstKTI GATC 3 cut(s) 219, 246, 250
BstMBI GATC 3 cut(s) 216, 243, 247
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 5 cut(s) 26, 47, 109, 118, 162
BstNSI RCATGY 1 cut(s) 269
BstV1I GCAGC 4 cut(s) 32, 67, 124, 143
BstV2I GAAGAC 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 119
CaiI CAGNNNCTG 2 cut(s) 16, 80
CciI TCATGA 1 cut(s) 369
CfoI GCGC 2 cut(s) 57, 117
Cfr13I GGNCC 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 2 cut(s) 86, 130
Csp6I GTAC 2 cut(s) 95, 172
CviAII CATG 2 cut(s) 266, 370
CviJI RGCY 9 cut(s) 41, 62, 103, 136, 156, 165, 257, 304, 392
CviKI_1 RGCY 9 cut(s) 41, 62, 103, 136, 156, 165, 257, 304, 392
CviQI GTAC 2 cut(s) 95, 172
DdeI CTNAG 1 cut(s) 305
DinI GGCGCC 1 cut(s) 56
DpnI GATC 3 cut(s) 218, 245, 249
DpnII GATC 3 cut(s) 216, 243, 247
EciI GGCGGA 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 56
EheI GGCGCC 1 cut(s) 56
FaeI CATG 2 cut(s) 269, 373
FaiI YATR 2 cut(s) 267, 371
FaqI GGGAC 2 cut(s) 133, 289
FatI CATG 2 cut(s) 265, 369
Fnu4HI GCNGC 8 cut(s) 21, 24, 27, 48, 81, 84, 113, 157
Fsp4HI GCNGC 8 cut(s) 21, 24, 27, 48, 81, 84, 113, 157
FspBI CTAG 2 cut(s) 90, 107
GlaI GCGC 2 cut(s) 56, 116
GluI GCNGC 8 cut(s) 21, 24, 27, 48, 81, 84, 113, 157
HaeII RGCGCY 2 cut(s) 58, 118
HapII CCGG 1 cut(s) 44
HgaI GACGC 2 cut(s) 86, 130
HhaI GCGC 2 cut(s) 57, 117
Hin1I GRCGYC 2 cut(s) 55, 141
Hin1II CATG 2 cut(s) 269, 373
Hin6I GCGC 2 cut(s) 55, 115
HinP1I GCGC 2 cut(s) 55, 115
HinfI GANTC 1 cut(s) 362
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 2 cut(s) 261, 313
Hpy188III TCNNGA 3 cut(s) 90, 150, 370
Hpy8I GTNNAC 1 cut(s) 174
Hpy99I CGWCG 1 cut(s) 212
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 47, 109, 118, 162
HpyF3I CTNAG 1 cut(s) 305
Hsp92I GRCGYC 2 cut(s) 55, 141
Hsp92II CATG 2 cut(s) 269, 373
HspAI GCGC 2 cut(s) 55, 115
KasI GGCGCC 1 cut(s) 54
Kzo9I GATC 3 cut(s) 216, 243, 247
LmnI GCTCC 3 cut(s) 46, 108, 389
LpnPI CCDG 6 cut(s) 2, 23, 57, 131, 292, 393
Lsp1109I GCAGC 4 cut(s) 32, 67, 124, 143
LweI GCATC 3 cut(s) 14, 95, 252
MaeI CTAG 2 cut(s) 90, 107
MalI GATC 3 cut(s) 218, 245, 249
MboI GATC 3 cut(s) 216, 243, 247
MboII GAAGA 3 cut(s) 233, 247, 292
MluCI AATT 1 cut(s) 181
Mly113I GGCGCC 1 cut(s) 55
MnlI CCTC 3 cut(s) 27, 45, 146
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 2 cut(s) 71, 412
MspA1I CMGCKG 2 cut(s) 23, 121
MspI CCGG 1 cut(s) 44
MwoI GCNNNNNNNGC 5 cut(s) 26, 47, 109, 118, 162
NarI GGCGCC 1 cut(s) 55
NdeII GATC 3 cut(s) 216, 243, 247
NlaIII CATG 2 cut(s) 269, 373
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 117
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PagI TCATGA 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 8 cut(s) 22, 25, 28, 49, 82, 85, 114, 158
PluTI GGCGCC 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 309
PstNI CAGNNNCTG 2 cut(s) 16, 80
RsaI GTAC 2 cut(s) 96, 173
RsaNI GTAC 2 cut(s) 95, 172
SaqAI TTAA 2 cut(s) 71, 412
SatI GCNGC 8 cut(s) 21, 24, 27, 48, 81, 84, 113, 157
Sau3AI GATC 3 cut(s) 216, 243, 247
Sau96I GGNCC 1 cut(s) 309
SetI ASST 7 cut(s) 43, 105, 138, 202, 306, 311, 394
SfaNI GCATC 3 cut(s) 14, 95, 252
SfoI GGCGCC 1 cut(s) 56
SinI GGWCC 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 1 cut(s) 181
SsiI CCGC 6 cut(s) 23, 26, 29, 47, 83, 121
SspDI GGCGCC 1 cut(s) 54
SspMI CTAG 2 cut(s) 90, 107
TaqI TCGA 3 cut(s) 207, 219, 246
TasI AATT 1 cut(s) 181
TauI GCSGC 4 cut(s) 26, 29, 50, 86
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 71, 412
Tru9I TTAA 2 cut(s) 71, 412
TseI GCWGC 4 cut(s) 20, 80, 112, 156
TspDTI ATGAA 1 cut(s) 358
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 309
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 1 cut(s) 269
XspI CTAG 2 cut(s) 90, 107
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.