Rw7G028370

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004631
Physical Location & Seq
Forward (+)
38585755 .. 38587342
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G028370.1

Sequence Viewer

Length: 327 bp
ATGGCTTCGCAGATTCTGGCTGCGGCGGCGGGGAGGACGGCAGCGGCGGCGAGGCGGCAAGCGCAGCGCCAGCGGTCGTATCTGAAGCTCGGGGTTAAGGCGCGTGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGGCGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGAACCAAGATGGATTTTGTGGATGACAAACTCAGGTCTGAGTTTACATTTATCAATCCCAACTCAAAAGGACAATGCGGCTGTGGTGAATCTTTTATGACAACAGGTAGTACTGGAGCTACTAAGCAAAGCAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

108

Amino Acids

11.5

Weight (kDa)

9.96

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 43 - 92 5.6e-07 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 103
AciI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 264
AclWI GGATC 1 cut(s) 155
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 2 cut(s) 125, 298
AluBI AGCT 2 cut(s) 88, 305
AluI AGCT 2 cut(s) 88, 305
AlwI GGATC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 89
ApeKI GCWGC 5 cut(s) 20, 41, 64, 108, 318
AspLEI GCGC 3 cut(s) 64, 69, 103
AsuHPI GGTGA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 89
BbvI GCAGC 4 cut(s) 7, 53, 76, 95
BccI CCATC 1 cut(s) 190
BceAI ACGGC 2 cut(s) 54, 130
BfoI RGCGCY 1 cut(s) 70
BmcAI AGTACT 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 190
BmsI GCATC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 159
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 155
BseMII CTCAG 2 cut(s) 216, 232
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 4 cut(s) 7, 53, 76, 95
Bsh1236I CGCG 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 77
BsiHKCI CYCGRG 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 89
Bsp143I GATC 2 cut(s) 156, 160
BspACI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 264
BspCNI CTCAG 2 cut(s) 217, 231
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 304
BssMI GATC 2 cut(s) 156, 160
BstC8I GCNNGC 3 cut(s) 60, 71, 142
BstDEI CTNAG 3 cut(s) 218, 225, 309
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 103
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 3 cut(s) 64, 69, 103
BstKTI GATC 2 cut(s) 159, 163
BstMBI GATC 2 cut(s) 156, 160
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
BstNSI RCATGY 1 cut(s) 182
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 4 cut(s) 7, 53, 76, 95
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 3 cut(s) 60, 71, 142
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 3 cut(s) 64, 69, 103
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 124, 297
CviAII CATG 1 cut(s) 179
CviJI RGCY 9 cut(s) 5, 20, 88, 108, 117, 170, 267, 305, 321
CviKI_1 RGCY 9 cut(s) 5, 20, 88, 108, 117, 170, 267, 305, 321
CviQI GTAC 2 cut(s) 124, 297
DdeI CTNAG 3 cut(s) 218, 225, 309
DpnI GATC 2 cut(s) 158, 162
DpnII GATC 2 cut(s) 156, 160
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 180
FaeI CATG 1 cut(s) 182
FaiI YATR 2 cut(s) 180, 284
FatI CATG 1 cut(s) 178
FauI CCCGC 1 cut(s) 22
FokI GGATG 1 cut(s) 221
GlaI GCGC 3 cut(s) 63, 68, 102
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 70
HhaI GCGC 3 cut(s) 64, 69, 103
Hin1II CATG 1 cut(s) 182
Hin6I GCGC 3 cut(s) 62, 67, 101
HinP1I GCGC 3 cut(s) 62, 67, 101
HinfI GANTC 2 cut(s) 13, 275
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 2 cut(s) 126, 231
Hpy188I TCNGA 3 cut(s) 84, 174, 226
Hpy8I GTNNAC 2 cut(s) 126, 231
HpyCH4V TGCA 2 cut(s) 111, 178
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
HpyF3I CTNAG 3 cut(s) 218, 225, 309
Hsp92II CATG 1 cut(s) 182
HspAI GCGC 3 cut(s) 62, 67, 101
Kzo9I GATC 2 cut(s) 156, 160
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 6 cut(s) 2, 83, 126, 205, 276, 285
Lsp1109I GCAGC 4 cut(s) 7, 53, 76, 95
LweI GCATC 1 cut(s) 165
MalI GATC 2 cut(s) 158, 162
MboI GATC 2 cut(s) 156, 160
MboII GAAGA 1 cut(s) 160
MluCI AATT 1 cut(s) 133
MnlI CCTC 2 cut(s) 27, 45
Mph1103I ATGCAT 1 cut(s) 180
MseI TTAA 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 44, 73
MteI GCGCNGCGC 1 cut(s) 65
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 6 cut(s) 26, 47, 61, 64, 70, 114
NdeII GATC 2 cut(s) 156, 160
NlaIII CATG 1 cut(s) 182
NlaIV GGNNCC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 180
NspI RCATGY 1 cut(s) 182
PfeI GAWTC 2 cut(s) 13, 275
PspN4I GGNNCC 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 125, 298
RsaNI GTAC 2 cut(s) 124, 297
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 2 cut(s) 156, 160
ScaI AGTACT 1 cut(s) 298
SetI ASST 4 cut(s) 90, 224, 295, 307
SfaNI GCATC 1 cut(s) 165
Sse9I AATT 1 cut(s) 133
SsiI CCGC 8 cut(s) 23, 26, 29, 44, 47, 55, 73, 264
TaqI TCGA 1 cut(s) 159
TasI AATT 1 cut(s) 133
TatI WGTACW 1 cut(s) 296
TauI GCSGC 6 cut(s) 26, 29, 47, 50, 58, 267
TfiI GAWTC 2 cut(s) 13, 275
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseI GCWGC 5 cut(s) 20, 41, 64, 108, 318
XceI RCATGY 1 cut(s) 182
ZrmI AGTACT 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.