Rorug03G0132000

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001662
Physical Location & Seq
Forward (+)
10704082 .. 10712503
8422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0132000.1

Sequence Viewer

Length: 2154 bp
ATGGACACTGTGGATCCTACGCGAGCTTTTGTCAAGAATGTTAAGCGTATCATCGTCAAGGTTGGAACTGCTGTGGTCACTCGTGGTGATGGAAGACTGGCATTGGGTAGACTGGGATCTCTTTGTGAGCAGCTTAAAGATATAAATTCTCAGGGCTATGAGGTTATTTTGGTGACCTCAGGAGCTGTTGGGCTTGGCCGTCAAAGGCTGAGATACAGGAGATTGGCAAATAGCAGCTTTGCTGATCTCCAAAACCCACAAAATGATTTTGATGGAAAGGCTTGTGCAGCTGTTGGACAGAGTAGTCTCATGGCTCTTTATGATGCCATGTTCAGCCAGCTTGATGTGACTTCTTCTCAGCTTCTCGTGACAGATAGTGTTTTCAGGGATGAAGCTTTCAGAAAGCAGCTCTCTGAAACTGTGAAGTCCTTGTTAAAACTGAGGGTTATTCCCATATTCAACGAAAATGATGCAGTTAGTACTAGGAAGGCCCCTTATGAGGACTCTTCTGGTATATTTTGGGACAATGATAGTTTGGCTGGTCTATTGGCCCTCGAACTGAAGGCTGATCTTCTTGTGTTGCTAAGTGATGTAGAGGGTCTCTACAGTGGTCCTCCCACTGATCCAAAGTCAAAGTTAATCCGTACATATGTGAAAGAAAAACATCATGGAGAAATAACATTTGGAGACAAGTCAAGGGTGGGCAGGGGGGGTATGACCGCTAAAGTCAATGCTGCTGTGTGTGCCGCTTATGGTGGAATCCCTGTTGTTATCACTAGTGGCTATGCTAATGATAACATTATCAAAGTGCTTCAAGGAGAACGAATTGGTACTGTCTTTCATCAAGATGCACATTTATGGACTCTGGTTAAAGAAGAATGTGCACGCGACTTGGCTGTTGCAGCACGGGAATGTTCTAGAAAGCTTCGAGCTCTAAACTGTCAGGATAGGAGGCAAATATTGCTCGATGTAGCTGATGCCTTGGAGGCAAATGAAAGTGACATTTTAGCTGAAAATGAAGCTGATATTGCAGCTGCGCAGGAAGCTGGATATGATGGGGCACTAATAGCTCGGTTGGCCCTAAAGCCTGGGAAGATTTCTGCACTTGCAAAATCTATGCGTCAACTTGCAGACATGGAAGAGCCCATTGGCCAGGTCTTGCAGAAAACAGAGGTTGCAGATGGACTTGTCTTAGAGAAAGTGACTTGTCCCTTGGGTGTTCTTTTGGTTGTTTTTGAGTCTCGACCTGATGCCCTTGTTCAGATAGCTTCATTAGCAATTCAAAGTCGGAATGGTCTTCTGCTTAAAGGTGGGAAAGAAGCCAAGAGGTCAAATGCTATCTTGCACAAGGTCATCACCAAAGCAATCCCAGATACTGTTGGTGGCGAGCTTATTGGACTTGTAACTTCAAGAGATGATATTCCTGATCTGCTGAAGCTCGACGATGTGATAGATCTTGTCATCCCTAGAGGCAGTAATAAACTCGTTTCTCAAATCAAGGAGTCGACAAAGATACCTGTTCTTGGTCATGCTGATGGGATCTGCCATGTTTATATCGATAAGTCAGCTAATTTGGAGATGGCAAAGCGCATTGTTCATGATGCAAAGACAGATTATCCTGCAGCCTGTAATGCAATGGAAACACTTCTTGTACACCAGGATTTGTTTAACAATGGTCAGCTTAATGAGATAATCACACAACTCCGAGAGTCAGGTGTTACTTTATATGGTGGACCACATGCAGCAGTTTTGTTAAATCTTTCAGAGACAAGCACATTGCATCACGAGTACAGTTCAATGGCTTGCACAGTTGAAATTGTAGATAATGTGCATGATGCAGTTGACCACATTCATGTATATGGAAGTGGTCACACTGAATGCATTGTTACTGAAGATGATGAAGTTGCTGAATATTTCTTAAGCCAAGTTGACAGCGCTGCAGTATTTCACAATGCAAGTACACGATTCTGCGACGGAGCTCGCTTTGGTCTTGGTGCAGAGGTTGGGATAAGTACAAGCAGGATTCACGCTCGAGGTCCTGTAGGAGTTGAAGGACTGCTAACAACCAGATGGACTCTGAGGGGGAAAGGGCAGATCGTGGATGGTGATAATGGGGTCGAATACACTCACAAGAATTTACTTAAAAGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

717

Amino Acids

77.63

Weight (kDa)

5.74

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 15 - 260 6e-36 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 303
Acc16I TGCGCA 1 cut(s) 1038
AccI GTMKAC 2 cut(s) 109, 1505
AccII CGCG 2 cut(s) 22, 888
AciI CCGC 2 cut(s) 720, 747
AclWI GGATC 5 cut(s) 8, 21, 124, 617, 1547
AcoI YGGCCR 2 cut(s) 196, 1150
AcsI RAATTY 2 cut(s) 145, 2134
AcuI CTGAAG 3 cut(s) 581, 1454, 1911
AfaI GTAC 7 cut(s) 481, 646, 832, 1653, 1790, 1960, 2014
AfeI AGCGCT 1 cut(s) 1936
AfiI CCNNNNNNNGG 2 cut(s) 499, 1523
AflII CTTAAG 1 cut(s) 1918
AgsI TTSAA 7 cut(s) 460, 815, 1283, 1410, 1797, 1814, 2051
AhlI ACTAGT 1 cut(s) 776
AjnI CCWGG 3 cut(s) 1087, 1152, 1656
AjuI GAANNNNNNNTTGG 4 cut(s) 666, 698, 1131, 1163
Alw21I GWGCWC 3 cut(s) 886, 934, 1981
Alw26I GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
Alw44I GTGCAC 1 cut(s) 882
AlwI GGATC 5 cut(s) 8, 21, 124, 617, 1547
AlwNI CAGNNNCTG 2 cut(s) 185, 1376
Ama87I CYCGRG 1 cut(s) 2031
Aor51HI AGCGCT 1 cut(s) 1936
AoxI GGCC 5 cut(s) 196, 489, 549, 1077, 1150
ApaLI GTGCAC 1 cut(s) 882
ApoI RAATTY 2 cut(s) 145, 2134
Asp700I GAANNNNTTC 1 cut(s) 1644
AspLEI GCGC 3 cut(s) 1039, 1590, 1937
AspS9I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
AsuHPI GGTGA 4 cut(s) 98, 184, 1348, 2117
AvaI CYCGRG 1 cut(s) 2031
AvaII GGWCC 3 cut(s) 611, 1733, 2036
AxyI CCTNAGG 1 cut(s) 178
BaeGI GKGCMC 2 cut(s) 886, 1063
BalI TGGCCA 1 cut(s) 1152
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 3 cut(s) 934, 1146, 1981
BauI CACGAG 3 cut(s) 81, 365, 1784
BbsI GAAGAC 2 cut(s) 100, 1289
Bbv12I GWGCWC 3 cut(s) 886, 934, 1981
BccI CCATC 8 cut(s) 83, 266, 1049, 1175, 1529, 1573, 2064, 2096
BceAI ACGGC 1 cut(s) 183
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 3 cut(s) 1089, 1154, 1658
BcoDI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BcuI ACTAGT 1 cut(s) 776
BfaI CTAG 4 cut(s) 483, 777, 918, 1467
BfmI CTRYAG 4 cut(s) 604, 1620, 1938, 2040
BfoI RGCGCY 1 cut(s) 1938
BfrI CTTAAG 1 cut(s) 1918
BglI GCCNNNNNGGC 1 cut(s) 986
BglII AGATCT 1 cut(s) 1453
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 3 cut(s) 1089, 1154, 1658
Bme18I GGWCC 3 cut(s) 611, 1733, 2036
BmeT110I CYCGRG 1 cut(s) 2031
BmgT120I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
BmiI GGNNCC 2 cut(s) 15, 492
BmrFI CCNGG 3 cut(s) 1089, 1154, 1658
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 2 cut(s) 100, 1289
Bsa29I ATCGAT 1 cut(s) 1557
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 981, 1088, 1212
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 1523
Bse1I ACTGG 2 cut(s) 102, 117
Bse21I CCTNAGG 1 cut(s) 178
Bse3DI GCAATG 2 cut(s) 1641, 1775
BseBI CCWGG 3 cut(s) 1089, 1154, 1658
BseCI ATCGAT 1 cut(s) 1557
BseDI CCNNGG 3 cut(s) 981, 1088, 1212
BseGI GGATG 3 cut(s) 394, 1461, 2107
BseLI CCNNNNNNNGG 2 cut(s) 499, 1523
BseMI GCAATG 2 cut(s) 1641, 1775
BseMII CTCAG 6 cut(s) 164, 192, 200, 371, 431, 2069
BseNI ACTGG 2 cut(s) 102, 117
BseSI GKGCMC 2 cut(s) 886, 1063
BsgI GTGCAG 3 cut(s) 306, 1086, 2016
Bsh1236I CGCG 2 cut(s) 22, 888
BshFI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BshVI ATCGAT 1 cut(s) 1557
BsiHKAI GWGCWC 3 cut(s) 886, 934, 1981
BsiHKCI CYCGRG 1 cut(s) 2031
BslFI GGGAC 2 cut(s) 536, 1194
BslI CCNNNNNNNGG 2 cut(s) 499, 1523
BsmAI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BsmFI GGGAC 2 cut(s) 536, 1194
BsmI GAATGC 1 cut(s) 1883
BsnI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
Bso31I GGTCTC 1 cut(s) 605
BsoBI CYCGRG 1 cut(s) 2031
Bsp1286I GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
Bsp1407I TGTACA 1 cut(s) 1651
Bsp143I GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
BspACI CCGC 2 cut(s) 720, 747
BspANI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BspCNI CTCAG 6 cut(s) 163, 191, 201, 370, 432, 2070
BspDI ATCGAT 1 cut(s) 1557
BspFNI CGCG 2 cut(s) 22, 888
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 2 cut(s) 15, 492
BspMAI CTGCAG 2 cut(s) 1624, 1942
BspPI GGATC 5 cut(s) 8, 21, 124, 617, 1547
BspQI GCTCTTC 1 cut(s) 1134
BspTI CTTAAG 1 cut(s) 1918
BspTNI GGTCTC 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 1641, 1775
BsrGI TGTACA 1 cut(s) 1651
BsrI ACTGG 2 cut(s) 102, 117
BssECI CCNNGG 3 cut(s) 981, 1088, 1212
BssMI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
BssSI CACGAG 3 cut(s) 81, 365, 1784
BssT1I CCWWGG 2 cut(s) 981, 1212
Bst2BI CACGAG 3 cut(s) 81, 365, 1784
Bst2UI CCWGG 3 cut(s) 1089, 1154, 1658
Bst4CI ACNGT 8 cut(s) 10, 421, 608, 835, 941, 1378, 1793, 1810
Bst6I CTCTTC 2 cut(s) 511, 1134
BstAFI CTTAAG 1 cut(s) 1918
BstAPI GCANNNNNTGC 1 cut(s) 961
BstAUI TGTACA 1 cut(s) 1651
BstC8I GCNNGC 6 cut(s) 24, 338, 886, 1388, 1804, 1981
BstDEI CTNAG 8 cut(s) 150, 178, 209, 357, 440, 584, 1192, 2078
BstEII GGTNACC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 394, 1461, 2107
BstFNI CGCG 2 cut(s) 22, 888
BstH2I RGCGCY 1 cut(s) 1938
BstHHI GCGC 3 cut(s) 1039, 1590, 1937
BstKTI GATC 9 cut(s) 16, 119, 247, 571, 625, 1429, 1456, 1542, 2097
BstMAI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BstMBI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
BstNI CCWGG 3 cut(s) 1089, 1154, 1658
BstNSI RCATGY 1 cut(s) 1742
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 3 cut(s) 1087, 1152, 1656
BstSFI CTRYAG 4 cut(s) 604, 1620, 1938, 2040
BstSLI GKGCMC 2 cut(s) 886, 1063
BstUI CGCG 2 cut(s) 22, 888
BstV2I GAAGAC 2 cut(s) 100, 1289
BstX2I RGATCY 4 cut(s) 13, 116, 1453, 1539
BstYI RGATCY 4 cut(s) 13, 116, 1453, 1539
Bsu15I ATCGAT 1 cut(s) 1557
Bsu36I CCTNAGG 1 cut(s) 178
BsuRI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BsuTUI ATCGAT 1 cut(s) 1557
BtsCI GGATG 3 cut(s) 394, 1461, 2107
BtsIMutI CAGTG 4 cut(s) 6, 613, 618, 1872
Cac8I GCNNGC 6 cut(s) 24, 338, 886, 1388, 1804, 1981
CaiI CAGNNNCTG 2 cut(s) 185, 1376
CciI TCATGA 1 cut(s) 1597
CfoI GCGC 3 cut(s) 1039, 1590, 1937
Cfr13I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
ClaI ATCGAT 1 cut(s) 1557
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
CviQI GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
DdeI CTNAG 8 cut(s) 150, 178, 209, 357, 440, 584, 1192, 2078
DpnI GATC 9 cut(s) 15, 118, 246, 570, 624, 1428, 1455, 1541, 2096
DpnII GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
DrdI GACNNNNNNGTC 1 cut(s) 303
DseDI GACNNNNNNGTC 1 cut(s) 303
EaeI YGGCCR 2 cut(s) 196, 1150
Eam1104I CTCTTC 2 cut(s) 511, 1134
EarI CTCTTC 2 cut(s) 511, 1134
Ecl136II GAGCTC 2 cut(s) 932, 1979
Eco130I CCWWGG 2 cut(s) 981, 1212
Eco24I GRGCYC 3 cut(s) 934, 1146, 1981
Eco31I GGTCTC 1 cut(s) 605
Eco47I GGWCC 3 cut(s) 611, 1733, 2036
Eco47III AGCGCT 1 cut(s) 1936
Eco53kI GAGCTC 2 cut(s) 932, 1979
Eco57I CTGAAG 3 cut(s) 581, 1454, 1911
Eco81I CCTNAGG 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 2031
Eco91I GGTNACC 1 cut(s) 172
EcoICRI GAGCTC 2 cut(s) 932, 1979
EcoO109I RGGNCCY 2 cut(s) 490, 2036
EcoO65I GGTNACC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 1087, 1152, 1656
EcoT14I CCWWGG 2 cut(s) 981, 1212
EcoT22I ATGCAT 1 cut(s) 1883
EcoT38I GRGCYC 3 cut(s) 934, 1146, 1981
ErhI CCWWGG 2 cut(s) 981, 1212
FaqI GGGAC 2 cut(s) 536, 1194
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 2 cut(s) 109, 1505
FokI GGATG 3 cut(s) 401, 1448, 2114
FriOI GRGCYC 3 cut(s) 934, 1146, 1981
FspBI CTAG 4 cut(s) 483, 777, 918, 1467
FspI TGCGCA 1 cut(s) 1038
GlaI GCGC 3 cut(s) 1038, 1589, 1936
HaeII RGCGCY 1 cut(s) 1938
HaeIII GGCC 5 cut(s) 198, 491, 551, 1079, 1152
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 3 cut(s) 1039, 1590, 1937
Hin6I GCGC 3 cut(s) 1037, 1588, 1935
HinP1I GCGC 3 cut(s) 1037, 1588, 1935
HincII GTYRAC 4 cut(s) 1124, 1506, 1843, 1930
HindII GTYRAC 4 cut(s) 1124, 1506, 1843, 1930
HindIII AAGCTT 2 cut(s) 393, 923
HinfI GANTC 9 cut(s) 503, 759, 862, 1238, 1502, 1709, 1965, 2023, 2074
HphI GGTGA 4 cut(s) 98, 184, 1348, 2117
Hpy166II GTNNAC 9 cut(s) 110, 884, 1124, 1506, 1654, 1733, 1843, 1930, 1961
Hpy188I TCNGA 7 cut(s) 401, 415, 1263, 1290, 1706, 1765, 2079
Hpy8I GTNNAC 9 cut(s) 110, 884, 1124, 1506, 1654, 1733, 1843, 1930, 1961
Hpy99I CGWCG 2 cut(s) 1445, 1976
HpyAV CCTTC 3 cut(s) 481, 556, 2045
HpyCH4III ACNGT 8 cut(s) 10, 421, 608, 835, 941, 1378, 1793, 1810
HpyF3I CTNAG 8 cut(s) 150, 178, 209, 357, 440, 584, 1192, 2078
HspAI GCGC 3 cut(s) 1037, 1588, 1935
Kzo9I GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
LguI GCTCTTC 1 cut(s) 1134
LmnI GCTCC 2 cut(s) 182, 1976
LweI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
MaeI CTAG 4 cut(s) 483, 777, 918, 1467
MalI GATC 9 cut(s) 15, 118, 246, 570, 624, 1428, 1455, 1541, 2096
MboI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
MboII GAAGA 9 cut(s) 105, 345, 498, 563, 887, 1105, 1151, 1289, 1904
MflI RGATCY 4 cut(s) 13, 116, 1453, 1539
MhlI GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
MlsI TGGCCA 1 cut(s) 1152
MluCI AATT 6 cut(s) 145, 825, 1278, 1570, 1815, 2134
MluNI TGGCCA 1 cut(s) 1152
MlyI GAGTC 6 cut(s) 497, 856, 1247, 1511, 1718, 2068
MmeI TCCRAC 3 cut(s) 43, 274, 1268
Mox20I TGGCCA 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 1883
MroXI GAANNNNTTC 1 cut(s) 1644
MscI TGGCCA 1 cut(s) 1152
MslI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
Msp20I TGGCCA 1 cut(s) 1152
MspA1I CMGCKG 2 cut(s) 290, 1034
MspCI CTTAAG 1 cut(s) 1918
MspR9I CCNGG 3 cut(s) 1089, 1154, 1658
Mva1269I GAATGC 1 cut(s) 1883
MvaI CCWGG 3 cut(s) 1089, 1154, 1658
MvnI CGCG 2 cut(s) 22, 888
NdeI CATATG 1 cut(s) 649
NdeII GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
NlaIV GGNNCC 2 cut(s) 15, 492
NmuCI GTSAC 7 cut(s) 76, 172, 346, 367, 998, 1201, 1868
NsbI TGCGCA 1 cut(s) 1038
NsiI ATGCAT 1 cut(s) 1883
NspI RCATGY 1 cut(s) 1742
PaeR7I CTCGAG 1 cut(s) 2031
PagI TCATGA 1 cut(s) 1597
PciSI GCTCTTC 1 cut(s) 1134
PctI GAATGC 1 cut(s) 1883
PdmI GAANNNNTTC 1 cut(s) 1644
PfeI GAWTC 3 cut(s) 759, 1965, 2023
PleI GAGTC 6 cut(s) 497, 856, 1246, 1510, 1717, 2068
PpsI GAGTC 6 cut(s) 497, 856, 1246, 1510, 1717, 2068
PpuMI RGGWCCY 1 cut(s) 2036
Psp124BI GAGCTC 2 cut(s) 934, 1981
Psp5II RGGWCCY 1 cut(s) 2036
Psp6I CCWGG 3 cut(s) 1087, 1152, 1656
PspEI GGTNACC 1 cut(s) 172
PspGI CCWGG 3 cut(s) 1087, 1152, 1656
PspN4I GGNNCC 2 cut(s) 15, 492
PspPI GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
PspPPI RGGWCCY 1 cut(s) 2036
PspXI VCTCGAGB 1 cut(s) 2031
PstI CTGCAG 2 cut(s) 1624, 1942
PstNI CAGNNNCTG 2 cut(s) 185, 1376
PsuI RGATCY 4 cut(s) 13, 116, 1453, 1539
PvuII CAGCTG 2 cut(s) 290, 1034
RsaI GTAC 7 cut(s) 481, 646, 832, 1653, 1790, 1960, 2014
RsaNI GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
RseI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
SacI GAGCTC 2 cut(s) 934, 1981
SalI GTCGAC 1 cut(s) 1504
SapI GCTCTTC 1 cut(s) 1134
Sau3AI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 2094
Sau96I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 6 cut(s) 497, 856, 1247, 1511, 1718, 2068
ScrFI CCNGG 3 cut(s) 1089, 1154, 1658
SduI GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
SfaNI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
SfcI CTRYAG 4 cut(s) 604, 1620, 1938, 2040
Sfr274I CTCGAG 1 cut(s) 2031
SinI GGWCC 3 cut(s) 611, 1733, 2036
SlaI CTCGAG 1 cut(s) 2031
SmiMI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
SmlI CTYRAG 2 cut(s) 1918, 2031
SmoI CTYRAG 2 cut(s) 1918, 2031
SpeI ACTAGT 1 cut(s) 776
Sse9I AATT 6 cut(s) 145, 825, 1278, 1570, 1815, 2134
SsiI CCGC 2 cut(s) 720, 747
SspI AATATT 2 cut(s) 960, 1913
SspMI CTAG 4 cut(s) 483, 777, 918, 1467
SstI GAGCTC 2 cut(s) 934, 1981
StyD4I CCNGG 3 cut(s) 1087, 1152, 1656
StyI CCWWGG 2 cut(s) 981, 1212
TaaI ACNGT 8 cut(s) 10, 421, 608, 835, 941, 1378, 1793, 1810
TaqI TCGA 9 cut(s) 555, 928, 966, 1243, 1440, 1505, 1557, 2032, 2118
TasI AATT 6 cut(s) 145, 825, 1278, 1570, 1815, 2134
TatI WGTACW 5 cut(s) 479, 1651, 1788, 1958, 2012
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 3 cut(s) 759, 1965, 2023
TscAI CASTG 4 cut(s) 13, 613, 625, 1879
TseFI GTSAC 7 cut(s) 76, 172, 346, 367, 998, 1201, 1868
Tsp45I GTSAC 7 cut(s) 76, 172, 346, 367, 998, 1201, 1868
TspDTI ATGAA 8 cut(s) 405, 830, 1008, 1032, 1260, 1586, 1841, 1914
TspGWI ACGGA 2 cut(s) 632, 1989
TspRI CASTG 4 cut(s) 13, 613, 625, 1879
Vha464I CTTAAG 1 cut(s) 1918
VneI GTGCAC 1 cut(s) 882
VpaK11BI GGWCC 3 cut(s) 611, 1733, 2036
XapI RAATTY 2 cut(s) 145, 2134
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 1 cut(s) 1742
XhoI CTCGAG 1 cut(s) 2031
XmiI GTMKAC 2 cut(s) 109, 1505
XmnI GAANNNNTTC 1 cut(s) 1644
XspI CTAG 4 cut(s) 483, 777, 918, 1467
ZrmI AGTACT 1 cut(s) 481
Zsp2I ATGCAT 1 cut(s) 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.