RLG00000024022

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001662
Physical Location & Seq
Reverse (-)
30946122 .. 30948655
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024022

Sequence Viewer

Length: 696 bp
ATGCCGGTGGAGTCTAGATTCAGGCTTGAAAGGCCGTTTGACATTGCCGAGGCCTTTTCTCAGAGGCGATCTGGCTCGCTTGCGGTTCTAATCGATGAGTCGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCCGCAGGAGATGATGGGCTTGCAAGGAGCAGAATGAGGACACCGAGAGCCGCGACTGGTCGAGAGAATGTGCCGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTGTGTTGCCTTCTTGGTATCCAAGAACCCCTCTCCGTGATATAACTGCGGTTACCAGGGCCATTGAGAGGAGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGGTCAAGTTCCATCTCTACAAGTTCAGGATGCTCCTCCTGAGCATCAAGTTCTCGCTGCTACTCCTTCAACCATTAAAAAGAGACCTTGCCCACCTCCTTCTGTTCTTAAAGTGCACAAAATTCTGCTAGAGGTGGCCAATCAGCCTGATGAGGGAGAGTTTGTTACACCAGAGAAGAAGCTGCTGAACTCGATTGACAAAGTTGAGAAGGTGGTGTTGGAAGAACTTCAGAGGCTGAAGAGGACGCCAAGTGCTAAGAAGGCAGAGAGGGAAAGAAAGGTTCGAACTTTGATGTCAATGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

25.91

Weight (kDa)

11.02

Isoelectric Point (pI)

68.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 212
AciI CCGC 6 cut(s) 83, 112, 162, 210, 233, 326
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 2 cut(s) 237, 525
AcsI RAATTY 1 cut(s) 510
AcuI CTGAAG 2 cut(s) 602, 647
AcyI GRCGYC 1 cut(s) 635
AfiI CCNNNNNNNGG 4 cut(s) 239, 259, 345, 542
AgeI ACCGGT 1 cut(s) 130
AgsI TTSAA 2 cut(s) 29, 459
AjnI CCWGG 1 cut(s) 332
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
AluBI AGCT 2 cut(s) 356, 571
AluI AGCT 2 cut(s) 356, 571
Alw21I GWGCWC 2 cut(s) 358, 507
Alw26I GTCTC 2 cut(s) 136, 466
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 625
AoxI GGCC 6 cut(s) 32, 51, 159, 237, 336, 525
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 446, 571
ApoI RAATTY 1 cut(s) 510
AsiGI ACCGGT 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 159, 336
AsuHPI GGTGA 1 cut(s) 377
AsuII TTCGAA 1 cut(s) 673
BaeGI GKGCMC 1 cut(s) 507
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 1 cut(s) 358
Bbv12I GWGCWC 2 cut(s) 358, 507
BbvI GCAGC 2 cut(s) 433, 558
BccI CCATC 2 cut(s) 167, 409
BceAI ACGGC 2 cut(s) 19, 161
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 334
BciVI GTATCC 1 cut(s) 306
BcoDI GTCTC 2 cut(s) 136, 466
BfaI CTAG 4 cut(s) 15, 149, 249, 518
BfmI CTRYAG 1 cut(s) 384
BfuI GTATCC 1 cut(s) 306
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 5 cut(s) 162, 210, 233, 447, 572
BlsI GCNGC 5 cut(s) 163, 211, 234, 448, 573
Bme1390I CCNGG 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 159, 336
BmrFI CCNGG 1 cut(s) 334
BmsI GCATC 3 cut(s) 145, 409, 442
BplI GAGNNNNNCTC 2 cut(s) 340, 372
Bpu10I CCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 673
Bsa29I ATCGAT 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 2 cut(s) 48, 333
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 259, 345, 542
Bse118I RCCGGY 3 cut(s) 4, 103, 130
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 334
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 48, 333
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 4 cut(s) 239, 259, 345, 542
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 74, 420
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 3 cut(s) 361, 383, 414
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 2 cut(s) 433, 558
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 6 cut(s) 34, 53, 161, 239, 338, 527
BshTI ACCGGT 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 2 cut(s) 358, 507
BsiSI CCGG 5 cut(s) 5, 104, 131, 240, 253
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 4 cut(s) 239, 259, 345, 542
BsmAI GTCTC 2 cut(s) 136, 466
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 276
BsnI GGCC 6 cut(s) 34, 53, 161, 239, 338, 527
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 358, 507
Bsp143I GATC 2 cut(s) 68, 269
BspACI CCGC 6 cut(s) 83, 112, 162, 210, 233, 326
BspANI GGCC 6 cut(s) 34, 53, 161, 239, 338, 527
BspCNI CTCAG 2 cut(s) 73, 421
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 346
BspT104I TTCGAA 1 cut(s) 673
BspTNI GGTCTC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 3 cut(s) 4, 103, 130
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 3 cut(s) 4, 103, 130
BssECI CCNNGG 2 cut(s) 48, 333
BssMI GATC 2 cut(s) 68, 269
BssNI GRCGYC 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 2 cut(s) 346, 623
BstACI GRCGYC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 673
BstC8I GCNNGC 6 cut(s) 77, 81, 105, 180, 237, 358
BstDEI CTNAG 3 cut(s) 60, 429, 645
BstEII GGTNACC 1 cut(s) 328
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 2 cut(s) 71, 272
BstMAI GTCTC 2 cut(s) 136, 466
BstMBI GATC 2 cut(s) 68, 269
BstMWI GCNNNNNNNGC 3 cut(s) 31, 109, 650
BstNI CCWGG 1 cut(s) 334
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 332
BstSFI CTRYAG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 433, 558
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 6 cut(s) 34, 53, 161, 239, 338, 527
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 6 cut(s) 77, 81, 105, 180, 237, 358
CaiI CAGNNNCTG 1 cut(s) 625
Cfr10I RCCGGY 3 cut(s) 4, 103, 130
Cfr13I GGNCC 2 cut(s) 159, 336
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 643
CspAI ACCGGT 1 cut(s) 130
DdeI CTNAG 3 cut(s) 60, 429, 645
DpnI GATC 2 cut(s) 70, 271
DpnII GATC 2 cut(s) 68, 269
EaeI YGGCCR 2 cut(s) 237, 525
Eam1104I CTCTTC 2 cut(s) 346, 623
EarI CTCTTC 2 cut(s) 346, 623
EciI GGCGGA 1 cut(s) 127
Ecl136II GAGCTC 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 2 cut(s) 602, 647
Eco91I GGTNACC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 356
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 136
FaiI YATR 2 cut(s) 157, 320
FaqI GGGAC 1 cut(s) 276
Fnu4HI GCNGC 5 cut(s) 162, 210, 233, 447, 572
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 5 cut(s) 162, 210, 233, 447, 572
FspBI CTAG 4 cut(s) 15, 149, 249, 518
GluI GCNGC 5 cut(s) 162, 210, 233, 447, 572
HaeIII GGCC 6 cut(s) 34, 53, 161, 239, 338, 527
HapII CCGG 5 cut(s) 5, 104, 131, 240, 253
HgaI GACGC 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 635
HinfI GANTC 3 cut(s) 11, 18, 98
HpaII CCGG 5 cut(s) 5, 104, 131, 240, 253
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 3 cut(s) 63, 141, 621
Hpy188III TCNNGA 4 cut(s) 15, 221, 416, 428
Hpy8I GTNNAC 1 cut(s) 505
HpyAV CCTTC 6 cut(s) 252, 297, 465, 498, 592, 643
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4V TGCA 4 cut(s) 136, 182, 386, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 109, 650
HpyF3I CTNAG 3 cut(s) 60, 429, 645
Hsp92I GRCGYC 1 cut(s) 635
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 68, 269
LguI GCTCTTC 1 cut(s) 346
LmnI GCTCC 2 cut(s) 186, 427
Lsp1109I GCAGC 2 cut(s) 433, 558
LweI GCATC 3 cut(s) 145, 409, 442
MaeI CTAG 4 cut(s) 15, 149, 249, 518
MaeIII GTNAC 2 cut(s) 328, 553
MalI GATC 2 cut(s) 70, 271
MboI GATC 2 cut(s) 68, 269
MboII GAAGA 4 cut(s) 363, 577, 623, 640
MhlI GDGCHC 2 cut(s) 358, 507
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 2 cut(s) 20, 107
MmeI TCCRAC 1 cut(s) 588
Mox20I TGGCCA 1 cut(s) 527
MreI CGCCGGCG 1 cut(s) 103
MroNI GCCGGC 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 615
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 465, 498
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 5 cut(s) 5, 104, 131, 240, 253
MspR9I CCNGG 1 cut(s) 334
MvaI CCWGG 1 cut(s) 334
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 3 cut(s) 31, 109, 650
NaeI GCCGGC 1 cut(s) 105
NdeII GATC 2 cut(s) 68, 269
NgoMIV GCCGGC 1 cut(s) 103
NmeAIII GCCGAG 1 cut(s) 73
NspV TTCGAA 1 cut(s) 673
PceI AGGCCT 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 346
PdiI GCCGGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 18
PinAI ACCGGT 1 cut(s) 130
PkrI GCNGC 5 cut(s) 163, 211, 234, 448, 573
PleI GAGTC 2 cut(s) 19, 106
PpsI GAGTC 2 cut(s) 19, 106
Psp124BI GAGCTC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 332
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 332
PspPI GGNCC 2 cut(s) 159, 336
PstI CTGCAG 1 cut(s) 388
PstNI CAGNNNCTG 1 cut(s) 625
SacI GAGCTC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 346
SaqAI TTAA 2 cut(s) 465, 498
SatI GCNGC 5 cut(s) 162, 210, 233, 447, 572
Sau3AI GATC 2 cut(s) 68, 269
Sau96I GGNCC 2 cut(s) 159, 336
SchI GAGTC 2 cut(s) 20, 107
ScrFI CCNGG 1 cut(s) 334
SduI GDGCHC 2 cut(s) 358, 507
SetI ASST 7 cut(s) 358, 478, 487, 525, 573, 603, 672
SfaNI GCATC 3 cut(s) 145, 409, 442
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 673
SgrAI CRCCGGYG 2 cut(s) 103, 130
Sse9I AATT 1 cut(s) 510
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 6 cut(s) 83, 112, 162, 210, 233, 326
SspMI CTAG 4 cut(s) 15, 149, 249, 518
SstI GAGCTC 1 cut(s) 358
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 332
TaaI ACNGT 1 cut(s) 278
TaqI TCGA 5 cut(s) 93, 220, 272, 581, 673
TasI AATT 1 cut(s) 510
TauI GCSGC 3 cut(s) 164, 212, 235
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 2 cut(s) 465, 498
Tru9I TTAA 2 cut(s) 465, 498
TscAI CASTG 1 cut(s) 283
TseI GCWGC 2 cut(s) 446, 571
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 1 cut(s) 283
VneI GTGCAC 1 cut(s) 503
XapI RAATTY 1 cut(s) 510
XbaI TCTAGA 1 cut(s) 14
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 4 cut(s) 15, 149, 249, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.