Rorug03G0132100

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000552
Physical Location & Seq
Reverse (-)
10713134 .. 10723768
10635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0132100.1

Sequence Viewer

Length: 3885 bp
ATGGCAACCAAAAGAACCTCCTCCTCAGTTTTTCCTTCTTCCTCTTCTTCATACCCACCTTGGACCTATGATGTCTTCCTCAGCTTCAGAGGTGAGGACACCCGTAAAAGTTTTACAGATCATCTATATTTTGCTTTGAAACGAGAAGGAATATCCACCTTTAGGGATGATGAACTTGAGAGAGGGAAACCAATTTCATCAGAACTCGTGCGAGCAATACAAGAATCAAGGTTTGCGCTTGTTATTTTCTCCAGAAACTATGCTTCTTCTAGGTGGTGCTTGGATGAACTCGCTGAGATTGTTGAGTGCATGAAAGAGATGGGACAGACGGTGCTTCCCATTTTCTATAATGTAGATCCATCAAATGTACGGAAACAAACGGGAAGCTTTGCAGAAGCCTTTGATGCACATGAAGAGCGCTTCAAGGATAACTTAGAAAAAGTGCAAAGGTGGAGAGCTGCTTTGACTGAAGTGGATCTTTCCGGATTTCATCTCCAAGATGGGCATGAGTCAGAATTTATTCAAAAGATTATTGAAAAGTTAGGATGTGATTTGGTTGGGGAACTCTCAATTGTTTCCAAAGACCTTGTTGGAATTGATTGCCGTGTAGAGGAAATAATGAAGTTATGTTCTGGTATAAGAAAGGAGAATGATGTGGGATTCATAGGTATATCAGGAATGGGTGGCATAGGAAAAACAACTCTTGCACAAGCTTTTTATGACAAAATCGCCAATCAATTTCCATCTAGAAGCTTTCTTCACAATGTAAGAGAGGTTTCAGAAAGAGATGGTATTGTTGCTTTACAAGAAAAACTCCTTCATGATGTTCGGATCAAAAGTTTTACACAAAGGTGGGATGTCCATGAGGGAATTAGTGGGATCAAGCACAGATTACGCCATAAAAAGGTCCTTATCATTCTTGATGATGTTGATAGTTTAGAACAATTGCAAGCATTAGCAGGAAGCGATGACTGGTTTGGTCAAGGGAGTAGGATCATTGTAACAACAAGAAATGAACATTTATTGAATGCTCATGGAGTGGATAGAAGGTATAAGGTTAAGGAATTGAGAAATGAGGAAGCCCTTCGGCTTTTTAGTTGGAAAGCCTTCAAGAGTGATTATCCTCAGAGAGATTACATGGAATTGTCCCTGGATTTTGTTAATTATGCCAAGGGCCTTCCTCTAGCACTTGAAGTTTTGGGTCCTTTCTTGTATGGTAAAAGCATAATTGAATGGAAAAGTGCCTTTAGTAGACTAAAACAAAGCCCTGAAGGAAAAATTATGTCTGTACTTCTAATAGGCTTTGATGGTTTGCATGAGAAGGAAAAGCAAATATTTTTAGACATTGCATGTTTCTTCAGAGGAAATGACAGAGAATATGTGGAAAAGATATTAGAAAGTTTTGGTTTTGACCCTGTCATTGGTCTAAGAGTTCTCATTGATAAATCTCTAATTACCGTAGTAGGGAAAATCTTGTGGATGCATGATTTAGTCCAGGAAATGGGTCGAGAAATTGTTCGTCAGGAAAATACAAGAGAGCTCGGAAAGCGCAGCAGATTGTGGCATTGTGATGATGTGCTTGATGTACTGGAGTATAATAAGGGAACCAAAGCAGTTGAAGGGTTAGTCCTAAAGTCACCTCAACAAAGAAAGGTACAATTGAACTGTGAAACCTTCTTGATGATGAAAAACCTAAGATTGCTTGGAATATATTGTGATGTGGTACTTCCTGAAGGCCTCAAGTATCTTCCTACTGAGTTGCGAGTTGTCCAATGGGAACGATATCCTCTGAAGTTATTTCCATCAAATTTCCCTTCAAGCAAACTTGTTGAACTGACCATGTGTTGCAGCAATATAAAACAATTATGGAAGGGATTCAAGGCATTAGAGAAGTTGAAATCTATTGAACTCACTGATTCTGAAAGTTTGACAGAAACCCCAGACTTTACCGGAGTCCCAAATCTTGAAAGGCTGATTCTTGAAGGTTGCACTAAATTATCTAGTGTTCATCCATCCATTAGAAATCTCAAGAAACTTGTCTTAATAAACATGAGGAATTGTAAAAGTCTTGAGAGCATTACTTGCAAGATCAGCATGGAATCTCTTGAAACTTTTATTCTTTCCGGTTGCGCCAAGCTCAAGAGATTTCCAGAGATTGTGGGAGATATGAAATGCTTGTCAAAGATTTGTTTAGATGGGACAGCTATACAAGAACTACCTTCGTCCATCGAGTTCCTGACTGGTCTTATGTTGTTGAATTTAAGTGATTGCAAAAATCTTCTTAGACTTCCGAGTGTCATTTACCATTTGACATCCCTTAAAACAATCAATCTATCAGGCTGCTCCAATCTTGATGAAATTCCAGTGACCTTAGGGGGTGTGGAAAGTCTGGAAGAGCTTGATGTGAGTGGAACTGCCATACGACAGATGCCATCATCCATTGTGTATCTCAAGAACCTCAAAGTGTTATCCTGTTCTGGGTGCAGAGGCATGTTGTCTACGTCATTGGTCTTCGGCTGGTTTCCAGCTCTATGCCCTGCAATGGGCTTGCCGTCACCTTTTTCACTTTTAGGTTTATGCTTTTTGACCGAACTAGATTTAAGTTACTGCAATTTATTTGATGGAGCAATCCCAAATGATTTGGACTGCTTATGCTCTTTGAAGTATCTATATCTAAGGGGAAACAATTTTAGAATTCTCCCCGAGAGCATCTGCCGACTTTCTAACCTTTGGGATCTGTGCTTGAGAGATTGTAGCAAATTGCAGTCACTGCCAAAACTTCCAATAACTGTGGAATACATTTGGACGGATGGTTGTACCTCACTGAGAAGTGATTCTGATCAGTATGAAGTATGGCCTTCAGGAGAGGATGGACAACTTGCTATTATTAAGCGCTCCATATCCCCAGAATGTTTGGTTATTATGCCAGAGTTGGAGTTCATAGCAGTGCTTCAAAGATATCATGAGATCAATGAGGTTTCAAGTCCACCGTTGAAAACAGCATATGGTGTTTCAGCTGGAGTTATCATTATTCCAGAGTGGTTTACTAATAAAAGCTACAGATCTTTTGTAACCATCCAGCTGCCTACAGAGTTATATAATAAAAGCAGGCCGTGGATAGGATATGCTCTGTATGTTTGTTTTCTAATGCACGATGACTTTGATACTGGATGTGATTTGAAAGTTATGCACGAGTTTGATTGTTACTTCAATACCAAAGAAGGTCCTCGTCAAAAAGAAACTTTACGTCACGAGCTAAATGTCAAAGACATTCCATTCACTGGGCCAAATGGATATTGGCTATACGTAACACATGCGTGGTTTTCTCAGCGTTTGAATGGCTTCGAGGAGTGCAGTAGCATTGAGGTTTTCACTAGAACTGATAGCACAGCTGTACAAGTGAAAATGAGTGGTACTCGTCTACTGAAGCACGAGCAAGATGTTTTAGGGTTTATCCAAGCAATAAAAAGCATTTTCATTGGTGTAAGAAGAAGTAGGCAGTTCAGGAAAGCGTATTCAGTTCAAGAAACTAAGTTGACCATGAAGGAACAAGGAGGGGAATCGACTTCAGGAGATTCAGATTGGTCCAGAAGTCATTTGCTCATGTCGACAGAGGATCAAGGCTGCACAATTGACTCTACTATCTCTCTAGAAGAACGCATGGAGTCACTGCCTTTAGATTTCTATGAGGGTCAACAATTAGGAGAGAAAACCTACTGCATAGTAGGCATCCCCTTGAATCCGCACACTGCATCTGCTTTGAAGGAAATCCTTCAGGTCTCTCTCTCAATCCTCTCTCATCCGACACACTCATACACAATAGCTAGACTAATTAAATATCATTATTTTTTGACAGGAATATTCTCATCGGTACGTAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1294

Amino Acids

147.2

Weight (kDa)

6.21

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 181 7.4e-56 TIR domain
TIR_2 PF13676 25 - 145 3.1e-16 TIR domain
NB-ARC PF00931 208 - 372 1.1e-22 NB-ARC domain
NACHT PF05729 224 - 364 2.2e-06 NACHT domain
WHD_ROQ1 PF23282 440 - 508 1.8e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 670 - 778 1.6e-07 Leucine-rich repeat region
LRR_13 PF23286 700 - 786 1.8e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36250 AT2G36250 AT2G36250 AT2G36250 AT3G52750 AT3G52750 AT3G52750 AT3G52750
fragaria_vesca FvH4_6g18610 FvH4_6g18610 FvH4_6g18610 FvH4_6g18610
malus_domestica MD12G1035600.v1.1 MD14G1022400.v1.1
prunus_persica Prupe.7G110900_v2.0.a1 Prupe.7G110900_v2.0.a1
pyrus_communis pycom12g02340 pycom14g02140
rosa_chinensis RchiOBHm_Chr3g0473191 RchiOBHm_Chr3g0473221 RchiOBHm_Chr3g0473231 RchiOBHm_Chr3g0473301 RchiOBHm_Chr3g0473311 RchiOBHm_Chr3g0473351 RchiOBHm_Chr3g0473401 RchiOBHm_Chr3g0473491 RchiOBHm_Chr3g0473541 RchiOBHm_Chr5g0044451 RchiOBHm_Chr7g0190991
rosa_laevigata RLG00000019977 RLG00000024009 RLG00000024012 RLG00000024015 RLG00000024018 RLG00000024019 RLG00000024021 RLG00000030788
rosa_multiflora Rmu_sc0000327.1_g000039 Rmu_sc0002075.1_g000002 Rmu_sc0003069.1_g000018 Rmu_sc0003069.1_g000050 Rmu_sc0003069.1_g000058 Rmu_sc0006792.1_g000029 Rmu_sc0011598.1_g000001 Rmu_sc0016164.1_g000018
rosa_roxburghii Rroxscaffold_2G00102410 Rroxscaffold_2G00102420 Rroxscaffold_4G00324300 Rroxscaffold_6G00408060 Rroxscaffold_6G00408100 Rroxscaffold_6G00408130 Rroxscaffold_6G00408200 Rroxscaffold_6G00408220 Rroxscaffold_6G00408230
rosa_rugosa Rorug03G0132100 Rorug03G0132500 Rorug03G0132600 Rorug03G0132900 Rorug05G0214500 Rorug06G0144900
rosa_samantha Rh3AG183800 Rh3AG184500 Rh3BG210300 Rh3BG210500 Rh3BG210800 Rh3BG211200 Rh3BG211600 Rh3BG211800 Rh3BG212000 Rh3BG212200 Rh3BG212400 Rh3BG212500 Rh3CG207400 Rh3CG207500 Rh3CG207700 Rh3CG209100 Rh3DG207400 Rh3DG208500 Rh5BG305900 Rh5DG315200
rosa_wichuraiana Rw0G008130 Rw0G008180 Rw0G014760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1501, 3281
AccI GTMKAC 4 cut(s) 1252, 2498, 3421, 3608
AccIII TCCGGA 1 cut(s) 482
AciI CCGC 1 cut(s) 3743
AclWI GGATC 7 cut(s) 350, 483, 839, 887, 1001, 2742, 3624
AcsI RAATTY 5 cut(s) 515, 1807, 2257, 2358, 2694
AfaI GTAC 9 cut(s) 369, 1290, 1587, 1656, 1725, 2818, 3396, 3415, 3873
AfeI AGCGCT 2 cut(s) 419, 2894
AjnI CCWGG 2 cut(s) 1149, 1494
AleI CACNNNNGTG 2 cut(s) 850, 3316
Alw21I GWGCWC 1 cut(s) 1542
Alw26I GTCTC 1 cut(s) 3784
AlwI GGATC 7 cut(s) 350, 483, 839, 887, 1001, 2742, 3624
AlwNI CAGNNNCTG 1 cut(s) 2770
Ama87I CYCGRG 1 cut(s) 2702
Aor13HI TCCGGA 1 cut(s) 482
Aor51HI AGCGCT 2 cut(s) 419, 2894
AoxI GGCC 5 cut(s) 1174, 1735, 2855, 3110, 3284
ApeKI GCWGC 6 cut(s) 458, 1551, 1848, 2340, 3082, 3624
ApoI RAATTY 5 cut(s) 515, 1807, 2257, 2358, 2694
Asp700I GAANNNNTTC 8 cut(s) 519, 1083, 1106, 1515, 1874, 2833, 3341, 3771
AspLEI GCGC 5 cut(s) 238, 420, 1551, 2132, 2895
AspS9I GGNCC 7 cut(s) 63, 907, 1174, 1202, 3224, 3284, 3585
AsuHPI GGTGA 3 cut(s) 104, 1629, 2547
AvaI CYCGRG 1 cut(s) 2702
AvaII GGWCC 5 cut(s) 63, 907, 1202, 3224, 3585
AxyI CCTNAGG 1 cut(s) 2371
BanII GRGCYC 1 cut(s) 1542
BauI CACGAG 4 cut(s) 206, 3191, 3251, 3431
BbsI GAAGAC 2 cut(s) 67, 2503
Bbv12I GWGCWC 1 cut(s) 1542
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 6 cut(s) 445, 1563, 1860, 2327, 3069, 3611
BceAI ACGGC 3 cut(s) 588, 2536, 3097
BciT130I CCWGG 2 cut(s) 1151, 1496
BclI TGATCA 1 cut(s) 2839
BcoDI GTCTC 1 cut(s) 3784
BfaI CTAG 8 cut(s) 270, 747, 1184, 2001, 2594, 3375, 3650, 3825
BfmI CTRYAG 2 cut(s) 3058, 3087
BfoI RGCGCY 2 cut(s) 421, 2896
BglII AGATCT 1 cut(s) 3062
BisI GCNGC 6 cut(s) 459, 1552, 1849, 2341, 3083, 3625
BlsI GCNGC 6 cut(s) 460, 1553, 1850, 2342, 3084, 3626
Bme1390I CCNGG 2 cut(s) 1151, 1496
Bme18I GGWCC 5 cut(s) 63, 907, 1202, 3224, 3585
BmeT110I CYCGRG 1 cut(s) 2702
BmgT120I GGNCC 7 cut(s) 63, 907, 1174, 1202, 3224, 3284, 3585
BmiI GGNNCC 2 cut(s) 1203, 1606
BmrFI CCNGG 2 cut(s) 1151, 1496
BmrI ACTGGG 1 cut(s) 3291
BmsI GCATC 6 cut(s) 394, 1470, 2418, 2718, 3738, 3761
BmuI ACTGGG 1 cut(s) 3291
BpiI GAAGAC 2 cut(s) 67, 2503
BplI GAGNNNNNCTC 2 cut(s) 3400, 3432
BpmI CTGGAG 3 cut(s) 235, 1610, 3039
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 7 cut(s) 197, 1724, 2012, 2090, 2123, 2435, 2764
BsaAI YACGTR 2 cut(s) 3307, 3875
BsaBI GATNNNNATC 1 cut(s) 2838
BsaI GGTCTC 1 cut(s) 3784
BsaJI CCNNGG 4 cut(s) 59, 1149, 1170, 3113
BsaWI WCCGGW 3 cut(s) 482, 1949, 2123
Bse1I ACTGG 6 cut(s) 977, 1593, 2245, 2363, 3172, 3286
Bse21I CCTNAGG 1 cut(s) 2371
Bse3DI GCAATG 2 cut(s) 1344, 2547
Bse8I GATNNNNATC 1 cut(s) 2838
BseAI TCCGGA 1 cut(s) 482
BseBI CCWGG 2 cut(s) 1151, 1496
BseDI CCNNGG 4 cut(s) 59, 1149, 1170, 3113
BseJI GATNNNNATC 1 cut(s) 2838
BseMI GCAATG 2 cut(s) 1344, 2547
BseMII CTCAG 7 cut(s) 39, 94, 285, 1139, 1746, 2816, 3341
BseNI ACTGG 6 cut(s) 977, 1593, 2245, 2363, 3172, 3286
BseRI GAGGAG 3 cut(s) 10, 13, 3362
BseXI GCAGC 6 cut(s) 445, 1563, 1860, 2327, 3069, 3611
BsgI GTGCAG 3 cut(s) 2503, 3373, 3610
BshFI GGCC 5 cut(s) 1176, 1737, 2857, 3112, 3286
BsiHKAI GWGCWC 1 cut(s) 1542
BsiHKCI CYCGRG 1 cut(s) 2702
BsiSI CCGG 3 cut(s) 483, 1950, 2124
BslFI GGGAC 4 cut(s) 336, 1132, 1940, 2212
BsmAI GTCTC 1 cut(s) 3784
BsmFI GGGAC 4 cut(s) 336, 1132, 1940, 2212
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 5 cut(s) 1176, 1737, 2857, 3112, 3286
Bso31I GGTCTC 1 cut(s) 3784
BsoBI CYCGRG 1 cut(s) 2702
Bsp1286I GDGCHC 1 cut(s) 1542
Bsp13I TCCGGA 1 cut(s) 482
Bsp1407I TGTACA 1 cut(s) 3394
BspACI CCGC 1 cut(s) 3743
BspANI GGCC 5 cut(s) 1176, 1737, 2857, 3112, 3286
BspCNI CTCAG 7 cut(s) 38, 93, 286, 1138, 1747, 2817, 3340
BspEI TCCGGA 1 cut(s) 482
BspHI TCATGA 2 cut(s) 820, 2962
BspLI GGNNCC 2 cut(s) 1203, 1606
BspPI GGATC 7 cut(s) 350, 483, 839, 887, 1001, 2742, 3624
BspQI GCTCTTC 2 cut(s) 408, 2388
BspTNI GGTCTC 1 cut(s) 3784
BsrDI GCAATG 2 cut(s) 1344, 2547
BsrGI TGTACA 1 cut(s) 3394
BsrI ACTGG 6 cut(s) 977, 1593, 2245, 2363, 3172, 3286
BssECI CCNNGG 4 cut(s) 59, 1149, 1170, 3113
BssSI CACGAG 4 cut(s) 206, 3191, 3251, 3431
BssT1I CCWWGG 2 cut(s) 59, 1170
Bst2BI CACGAG 4 cut(s) 206, 3191, 3251, 3431
Bst2UI CCWGG 2 cut(s) 1151, 1496
Bst4CI ACNGT 5 cut(s) 331, 1459, 1667, 2791, 2991
Bst6I CTCTTC 3 cut(s) 49, 408, 2388
BstAPI GCANNNNNTGC 2 cut(s) 2082, 2770
BstAUI TGTACA 1 cut(s) 3394
BstBAI YACGTR 2 cut(s) 3307, 3875
BstC8I GCNNGC 4 cut(s) 213, 951, 2549, 3110
BstDSI CCRYGG 1 cut(s) 3113
BstH2I RGCGCY 2 cut(s) 421, 2896
BstHHI GCGC 5 cut(s) 238, 420, 1551, 2132, 2895
BstMAI GTCTC 1 cut(s) 3784
BstMWI GCNNNNNNNGC 6 cut(s) 404, 1546, 2082, 2091, 2770, 3726
BstNI CCWGG 2 cut(s) 1151, 1496
BstNSI RCATGY 3 cut(s) 1353, 2494, 3317
BstSCI CCNGG 2 cut(s) 1149, 1494
BstSFI CTRYAG 2 cut(s) 3058, 3087
BstSNI TACGTA 2 cut(s) 3307, 3875
BstV1I GCAGC 6 cut(s) 445, 1563, 1860, 2327, 3069, 3611
BstV2I GAAGAC 2 cut(s) 67, 2503
BstX2I RGATCY 4 cut(s) 355, 475, 2734, 3062
BstYI RGATCY 4 cut(s) 355, 475, 2734, 3062
Bsu36I CCTNAGG 1 cut(s) 2371
BsuRI GGCC 5 cut(s) 1176, 1737, 2857, 3112, 3286
BtgI CCRYGG 1 cut(s) 3113
BtgZI GCGATG 1 cut(s) 981
BtsI GCAGTG 4 cut(s) 2768, 2952, 3668, 3747
BtsIMutI CAGTG 8 cut(s) 1911, 2370, 2768, 2822, 2952, 3279, 3668, 3747
Cac8I GCNNGC 4 cut(s) 213, 951, 2549, 3110
CaiI CAGNNNCTG 1 cut(s) 2770
CciI TCATGA 2 cut(s) 820, 2962
CfoI GCGC 5 cut(s) 238, 420, 1551, 2132, 2895
Cfr13I GGNCC 7 cut(s) 63, 907, 1174, 1202, 3224, 3284, 3585
Csp6I GTAC 9 cut(s) 368, 1289, 1586, 1655, 1724, 2817, 3395, 3414, 3872
CspCI CAANNNNNGTGG 2 cut(s) 2772, 2807
CviQI GTAC 9 cut(s) 368, 1289, 1586, 1655, 1724, 2817, 3395, 3414, 3872
Eam1104I CTCTTC 3 cut(s) 49, 408, 2388
EarI CTCTTC 3 cut(s) 49, 408, 2388
Ecl136II GAGCTC 1 cut(s) 1540
Eco105I TACGTA 2 cut(s) 3307, 3875
Eco130I CCWWGG 2 cut(s) 59, 1170
Eco147I AGGCCT 1 cut(s) 1737
Eco24I GRGCYC 1 cut(s) 1542
Eco31I GGTCTC 1 cut(s) 3784
Eco32I GATATC 2 cut(s) 1784, 2960
Eco47I GGWCC 5 cut(s) 63, 907, 1202, 3224, 3585
Eco47III AGCGCT 2 cut(s) 419, 2894
Eco53kI GAGCTC 1 cut(s) 1540
Eco81I CCTNAGG 1 cut(s) 2371
Eco88I CYCGRG 1 cut(s) 2702
EcoICRI GAGCTC 1 cut(s) 1540
EcoO109I RGGNCCY 4 cut(s) 907, 1174, 1202, 3224
EcoRI GAATTC 1 cut(s) 2694
EcoRII CCWGG 2 cut(s) 1149, 1494
EcoRV GATATC 2 cut(s) 1784, 2960
EcoT14I CCWWGG 2 cut(s) 59, 1170
EcoT22I ATGCAT 2 cut(s) 1485, 3883
EcoT38I GRGCYC 1 cut(s) 1542
ErhI CCWWGG 2 cut(s) 59, 1170
FalI AAGNNNNNCTT 4 cut(s) 416, 448, 462, 494
FaqI GGGAC 4 cut(s) 336, 1132, 1940, 2212
FauNDI CATATG 1 cut(s) 3004
FbaI TGATCA 1 cut(s) 2839
FblI GTMKAC 4 cut(s) 1252, 2498, 3421, 3608
Fnu4HI GCNGC 6 cut(s) 459, 1552, 1849, 2341, 3083, 3625
FriOI GRGCYC 1 cut(s) 1542
Fsp4HI GCNGC 6 cut(s) 459, 1552, 1849, 2341, 3083, 3625
FspBI CTAG 8 cut(s) 270, 747, 1184, 2001, 2594, 3375, 3650, 3825
GlaI GCGC 5 cut(s) 237, 419, 1550, 2131, 2894
GluI GCNGC 6 cut(s) 459, 1552, 1849, 2341, 3083, 3625
GsuI CTGGAG 3 cut(s) 235, 1610, 3039
HaeII RGCGCY 2 cut(s) 421, 2896
HaeIII GGCC 5 cut(s) 1176, 1737, 2857, 3112, 3286
HapII CCGG 3 cut(s) 483, 1950, 2124
HhaI GCGC 5 cut(s) 238, 420, 1551, 2132, 2895
Hin6I GCGC 5 cut(s) 236, 418, 1549, 2130, 2893
HinP1I GCGC 5 cut(s) 236, 418, 1549, 2130, 2893
HincII GTYRAC 3 cut(s) 3537, 3609, 3695
HindII GTYRAC 3 cut(s) 3537, 3609, 3695
HindIII AAGCTT 3 cut(s) 385, 711, 751
HpaII CCGG 3 cut(s) 483, 1950, 2124
HphI GGTGA 3 cut(s) 104, 1629, 2547
Hpy166II GTNNAC 8 cut(s) 1253, 2499, 2987, 3045, 3422, 3537, 3609, 3695
Hpy8I GTNNAC 8 cut(s) 1253, 2499, 2987, 3045, 3422, 3537, 3609, 3695
HpyCH4III ACNGT 5 cut(s) 331, 1459, 1667, 2791, 2991
HpyCH4IV ACGT 4 cut(s) 2501, 3247, 3306, 3874
HpyF10VI GCNNNNNNNGC 6 cut(s) 404, 1546, 2082, 2091, 2770, 3726
HpySE526I ACGT 4 cut(s) 2501, 3247, 3306, 3874
HspAI GCGC 5 cut(s) 236, 418, 1549, 2130, 2893
Kpn2I TCCGGA 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 2839
LguI GCTCTTC 2 cut(s) 408, 2388
LmnI GCTCC 3 cut(s) 2348, 2624, 2900
Lsp1109I GCAGC 6 cut(s) 445, 1563, 1860, 2327, 3069, 3611
LweI GCATC 6 cut(s) 394, 1470, 2418, 2718, 3738, 3761
MaeI CTAG 8 cut(s) 270, 747, 1184, 2001, 2594, 3375, 3650, 3825
MaeII ACGT 4 cut(s) 2501, 3247, 3306, 3874
MfeI CAATTG 4 cut(s) 570, 944, 1658, 3630
MflI RGATCY 4 cut(s) 355, 475, 2734, 3062
MhlI GDGCHC 1 cut(s) 1542
MlyI GAGTC 4 cut(s) 518, 1962, 3629, 3674
MmeI TCCRAC 4 cut(s) 571, 1079, 2913, 3827
Mph1103I ATGCAT 2 cut(s) 1485, 3883
MroI TCCGGA 1 cut(s) 482
MroXI GAANNNNTTC 8 cut(s) 519, 1083, 1106, 1515, 1874, 2833, 3341, 3771
MseI TTAA 8 cut(s) 1059, 1161, 2042, 2261, 2319, 2600, 2889, 3834
MslI CAYNNNNRTG 4 cut(s) 850, 1569, 2945, 3316
MspA1I CMGCKG 3 cut(s) 3017, 3082, 3392
MspI CCGG 3 cut(s) 483, 1950, 2124
MspR9I CCNGG 2 cut(s) 1151, 1496
MunI CAATTG 4 cut(s) 570, 944, 1658, 3630
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 2 cut(s) 1151, 1496
MwoI GCNNNNNNNGC 6 cut(s) 404, 1546, 2082, 2091, 2770, 3726
NdeI CATATG 1 cut(s) 3004
NlaIV GGNNCC 2 cut(s) 1203, 1606
NmuCI GTSAC 6 cut(s) 1635, 2365, 2553, 2766, 3248, 3666
NsiI ATGCAT 2 cut(s) 1485, 3883
NspI RCATGY 3 cut(s) 1353, 2494, 3317
OliI CACNNNNGTG 2 cut(s) 850, 3316
PagI TCATGA 2 cut(s) 820, 2962
PceI AGGCCT 1 cut(s) 1737
PciSI GCTCTTC 2 cut(s) 408, 2388
PctI GAATGC 1 cut(s) 1033
PdmI GAANNNNTTC 8 cut(s) 519, 1083, 1106, 1515, 1874, 2833, 3341, 3771
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 2 cut(s) 1501, 3281
PfoI TCCNGGA 1 cut(s) 1494
PkrI GCNGC 6 cut(s) 460, 1553, 1850, 2342, 3084, 3626
PleI GAGTC 4 cut(s) 517, 1961, 3629, 3673
PpsI GAGTC 4 cut(s) 517, 1961, 3629, 3673
Ppu21I YACGTR 2 cut(s) 3307, 3875
PpuMI RGGWCCY 3 cut(s) 907, 1202, 3224
Psp124BI GAGCTC 1 cut(s) 1542
Psp5II RGGWCCY 3 cut(s) 907, 1202, 3224
Psp6I CCWGG 2 cut(s) 1149, 1494
PspGI CCWGG 2 cut(s) 1149, 1494
PspN4I GGNNCC 2 cut(s) 1203, 1606
PspPI GGNCC 7 cut(s) 63, 907, 1174, 1202, 3224, 3284, 3585
PspPPI RGGWCCY 3 cut(s) 907, 1202, 3224
PstNI CAGNNNCTG 1 cut(s) 2770
PsuI RGATCY 4 cut(s) 355, 475, 2734, 3062
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 3 cut(s) 3017, 3082, 3392
RsaI GTAC 9 cut(s) 369, 1290, 1587, 1656, 1725, 2818, 3396, 3415, 3873
RsaNI GTAC 9 cut(s) 368, 1289, 1586, 1655, 1724, 2817, 3395, 3414, 3872
RseI CAYNNNNRTG 4 cut(s) 850, 1569, 2945, 3316
SacI GAGCTC 1 cut(s) 1542
SalI GTCGAC 1 cut(s) 3607
SapI GCTCTTC 2 cut(s) 408, 2388
SaqAI TTAA 8 cut(s) 1059, 1161, 2042, 2261, 2319, 2600, 2889, 3834
SatI GCNGC 6 cut(s) 459, 1552, 1849, 2341, 3083, 3625
Sau96I GGNCC 7 cut(s) 63, 907, 1174, 1202, 3224, 3284, 3585
SchI GAGTC 4 cut(s) 518, 1962, 3629, 3674
ScrFI CCNGG 2 cut(s) 1151, 1496
SduI GDGCHC 1 cut(s) 1542
SfaNI GCATC 6 cut(s) 394, 1470, 2418, 2718, 3738, 3761
SfcI CTRYAG 2 cut(s) 3058, 3087
SinI GGWCC 5 cut(s) 63, 907, 1202, 3224, 3585
SmiMI CAYNNNNRTG 4 cut(s) 850, 1569, 2945, 3316
SmlI CTYRAG 7 cut(s) 176, 1739, 2027, 2069, 2138, 2450, 2743
SmoI CTYRAG 7 cut(s) 176, 1739, 2027, 2069, 2138, 2450, 2743
SnaBI TACGTA 2 cut(s) 3307, 3875
SseBI AGGCCT 1 cut(s) 1737
SsiI CCGC 1 cut(s) 3743
SspI AATATT 2 cut(s) 1335, 3861
SspMI CTAG 8 cut(s) 270, 747, 1184, 2001, 2594, 3375, 3650, 3825
SstI GAGCTC 1 cut(s) 1542
StuI AGGCCT 1 cut(s) 1737
StyD4I CCNGG 2 cut(s) 1149, 1494
StyI CCWWGG 2 cut(s) 59, 1170
TaaI ACNGT 5 cut(s) 331, 1459, 1667, 2791, 2991
TaiI ACGT 4 cut(s) 2504, 3250, 3309, 3877
TaqI TCGA 5 cut(s) 1507, 2229, 3345, 3563, 3608
TaqII GACCGA 1 cut(s) 2603
TatI WGTACW 3 cut(s) 1288, 1585, 3394
Tru1I TTAA 8 cut(s) 1059, 1161, 2042, 2261, 2319, 2600, 2889, 3834
Tru9I TTAA 8 cut(s) 1059, 1161, 2042, 2261, 2319, 2600, 2889, 3834
TscAI CASTG 8 cut(s) 1918, 2370, 2775, 2829, 2952, 3286, 3675, 3754
TseFI GTSAC 6 cut(s) 1635, 2365, 2553, 2766, 3248, 3666
TseI GCWGC 6 cut(s) 458, 1551, 1848, 2340, 3082, 3624
Tsp45I GTSAC 6 cut(s) 1635, 2365, 2553, 2766, 3248, 3666
TspGWI ACGGA 2 cut(s) 385, 2822
TspRI CASTG 8 cut(s) 1918, 2370, 2775, 2829, 2952, 3286, 3675, 3754
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 2 cut(s) 1501, 3281
VpaK11BI GGWCC 5 cut(s) 63, 907, 1202, 3224, 3585
XapI RAATTY 5 cut(s) 515, 1807, 2257, 2358, 2694
XbaI TCTAGA 2 cut(s) 746, 3649
XceI RCATGY 3 cut(s) 1353, 2494, 3317
XcmI CCANNNNNNNNNTGG 1 cut(s) 3294
XmiI GTMKAC 4 cut(s) 1252, 2498, 3421, 3608
XmnI GAANNNNTTC 8 cut(s) 519, 1083, 1106, 1515, 1874, 2833, 3341, 3771
XspI CTAG 8 cut(s) 270, 747, 1184, 2001, 2594, 3375, 3650, 3825
Zsp2I ATGCAT 2 cut(s) 1485, 3883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.