Rorug06G0144900

Acyl-coenzyme A thioesterase 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000552
Physical Location & Seq
Forward (+)
20667363 .. 20673178
5816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0144900.1

Sequence Viewer

Length: 2325 bp
ATGGCTCCTCCTCCAGGGATTGTTGTCACCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTCCTTCTTTCTTTTGCTTCCACAAATGTTTACATTGTTTATATGGGAGAAAGAAAGTATGGAAACCCTGAGCTGGTATCTGAGTCTCACCATGAGATTCTGTCATATGTATTTGGAAGCAAAGAAGCTGCAAGGGAATCAATTCTGTACACCTACAAGCATGGATTTTCGGGGTTCGCTGCAGTTCTAACTCCATCTCAAGCCAACCATATTGCAGATGTACCCGGTGTTGTTCGTGTCATTCCTCACAGGTTTCTTGATTTGCATACAACTAGAAGTTGGAACTTTCTGCAAGTAGACTCTCACTTTAGAACTGGAGTTCTTTCGAGGAGTCAATCAGGCATTGGGTCTATTATTGGAGTCATGGACACTGGAATTTGGCCCGAGTCTCAAAGCTTTAGAGATGAGGGTATGGGAGACATTCCATCTCGTTGGAGAGGCATATGTCAAGAAGGAGAAAAGTTCAATCACTCCCATTGCAACAGGAAGATAATTGGTGCACGCTGGTATAATAAAGGATATGAAGCTGAATTTGGAAAGATAGATACAAGTGATGGGTTTGAATTTCTATCTCCCCGAGATGCTTCAGGACATGGTACTCATACATCATCCACTGCAGCCGGTGGTTTTACAGAAAATGCAAGCTTTATGGGACTTGCTTCAGGATTGGCAAGAGGAGGTGCTCCATCATCTTGGTTAGCTATTTACAAAGTTTGTTGGGCTACTGGTGGCTGCAGCTCAGCAGACCTTCTTGCTGCATTTGATGACGCAATTTTTGATGGTGTTGACGTGCTTTCAGTATCTCTAGGTTCTCCGCCTCCACTTGCATCTTATGTTGAGGATGTTGTGTCCATTGGTTCTTTTCATGCTGTGGCCAAAGGAATCTCAGTTGTATGTTCTGCTGGAAACTCTGGCCCATACTCTCAAACGATCATAAATTCTGCTCCTTGGATATTCACTGTTGCAGCAAGCACTATCGACAGGGCTTTTCCTACTGCAATCTCCCTGGGAAACAATCAAACTGTAGTGGGTCAGGGTTTGTATACAGGGATGGATGTGAACAAGTTCTACCCTCTTGTGTATGGACAAGACATTGCATCTGTTGATGCAGATGAAGACAGCGCTGGGAGCTGTGAGTCGGGAACTCTAAATGGTACTTTAGCAAATGGAAAGTTTGTTCTTTGTTTCCAATCTCGATCACAGAGGTCATCCACTGCTGCACAGAGAACTGTAATGAGTGCCGGGGGTGCCGGTCTCATTTTTGCTCAGTTTCCCAGCAAGGATGTTTCTTTGTCCTCGGGTAGTCTACCGTGTGTTCAAATTGACTTTGCAATCGGCACATATATTCTCACATATATTGGAGCAACCAGAAATCCAATAGTCAAGTTAAACCCTACAAAAACACTATTGGGACAGCAAATATCCCCAGAAGTAGCATTCTTCTCTGCTCGAGGACCCAATTCTGTCTCTCCTTCGGTACTGAAGCCCGATATTGCTGCTCCTGGTGTGAATATATTGTCCTCTTGGTCCCCTGCTTATTCACTATTACAACATCAACCATCTCCAGTCATCTTTAAAATTGAATCAGGAACTTCCATGGCTTGTCCACATATATCTGGTATAGTGGCTCTTCTTAAAGTCATCCACCCAACATGGAGCCCTGCAGCAATAAAGTCTGCCCTTATTACAACAGCCTCTTTAGAAGATCAATATGGTCAAAGTATCGAGGCTGAGGGCGCTCCTCAGAAGAAAGCTGATCCATTCGACTATGGTGGTGGACATGTCAATCCTAACAAAGCCATAGCCCCCGGGCTCATTTATGATATAGAAACCTCAGATTACATCCGTTTTCTTTGCTCCATGGGGTATAACAATTCAGCCATCAGCGCAATCGCAGGAGCTAGCATCACATGCCATAAATCGACCAATGTCCAAGTTAATCTCAATCTGCCTTCCATCACCATTCATGAGCTCAAGGAAAGGATGACAGTATCAAGAACAGTCACAAATGTTGGTCCAGTTAATTCTATCTACATTGCTCGTGTTCAAACACCTGCCGGTGTGTCTGTTAAAGTTAAGCCATCGGCTTTGTTGTTCAACTCTACTGCAAAGAAGCTGGAGTTCAAGGTTGTTTTTCGTCCCCTGCTAAAAGTTGCAGGAAGATTTTCTTTTGGAAATCTGTACTGGGAAGATGGTTTTCATGTAGTGAGGATACCTCTAACAGTGAGAACTGTCCTTGATGATTTTTATGCAGAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

774

Amino Acids

82.75

Weight (kDa)

6.68

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 110 6.5e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 591 1.5e-43 Subtilase family
fn3_6 PF17766 668 - 763 5.5e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36250 AT2G36250 AT2G36250 AT2G36250 AT3G52750 AT3G52750 AT3G52750 AT3G52750
fragaria_vesca FvH4_6g18610 FvH4_6g18610 FvH4_6g18610 FvH4_6g18610
malus_domestica MD12G1035600.v1.1 MD14G1022400.v1.1
prunus_persica Prupe.7G110900_v2.0.a1 Prupe.7G110900_v2.0.a1
pyrus_communis pycom12g02340 pycom14g02140
rosa_chinensis RchiOBHm_Chr3g0473191 RchiOBHm_Chr3g0473221 RchiOBHm_Chr3g0473231 RchiOBHm_Chr3g0473301 RchiOBHm_Chr3g0473311 RchiOBHm_Chr3g0473351 RchiOBHm_Chr3g0473401 RchiOBHm_Chr3g0473491 RchiOBHm_Chr3g0473541 RchiOBHm_Chr5g0044451 RchiOBHm_Chr7g0190991
rosa_laevigata RLG00000019977 RLG00000024009 RLG00000024012 RLG00000024015 RLG00000024018 RLG00000024019 RLG00000024021 RLG00000030788
rosa_multiflora Rmu_sc0000327.1_g000039 Rmu_sc0002075.1_g000002 Rmu_sc0003069.1_g000018 Rmu_sc0003069.1_g000050 Rmu_sc0003069.1_g000058 Rmu_sc0006792.1_g000029 Rmu_sc0011598.1_g000001 Rmu_sc0016164.1_g000018
rosa_roxburghii Rroxscaffold_2G00102410 Rroxscaffold_2G00102420 Rroxscaffold_4G00324300 Rroxscaffold_6G00408060 Rroxscaffold_6G00408100 Rroxscaffold_6G00408130 Rroxscaffold_6G00408200 Rroxscaffold_6G00408220 Rroxscaffold_6G00408230
rosa_rugosa Rorug03G0132100 Rorug03G0132500 Rorug03G0132600 Rorug03G0132900 Rorug05G0214500 Rorug06G0144900
rosa_samantha Rh3AG183800 Rh3AG184500 Rh3BG210300 Rh3BG210500 Rh3BG210800 Rh3BG211200 Rh3BG211600 Rh3BG211800 Rh3BG212000 Rh3BG212200 Rh3BG212400 Rh3BG212500 Rh3CG207400 Rh3CG207500 Rh3CG207700 Rh3CG209100 Rh3DG207400 Rh3DG208500 Rh5BG305900 Rh5DG315200
rosa_wichuraiana Rw0G008130 Rw0G008180 Rw0G014760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2125
Acc36I ACCTGC 1 cut(s) 2125
AccB1I GGYRCC 1 cut(s) 1310
AccI GTMKAC 3 cut(s) 360, 1106, 1369
AciI CCGC 1 cut(s) 878
AclWI GGATC 1 cut(s) 1814
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 4 cut(s) 438, 593, 626, 1000
AcuI CTGAAG 3 cut(s) 633, 708, 1565
AfaI GTAC 6 cut(s) 212, 285, 661, 1219, 1542, 2246
AfeI AGCGCT 1 cut(s) 1186
AfiI CCNNNNNNNGG 2 cut(s) 14, 136
AflIII ACRYGT 1 cut(s) 1843
AgsI TTSAA 7 cut(s) 529, 626, 1382, 1646, 2111, 2161, 2188
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 3 cut(s) 13, 1068, 1564
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AloI GAACNNNNNNTCC 2 cut(s) 1224, 1256
Alw21I GWGCWC 3 cut(s) 565, 748, 2037
Alw26I GTCTC 6 cut(s) 153, 456, 474, 1322, 1534, 2312
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 1 cut(s) 1814
Ama87I CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
Aor51HI AGCGCT 1 cut(s) 1186
AoxI GGCC 3 cut(s) 443, 936, 976
ApaLI GTGCAC 1 cut(s) 561
ApoI RAATTY 4 cut(s) 438, 593, 626, 1000
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
Asp700I GAANNNNTTC 3 cut(s) 204, 1127, 2227
AspLEI GCGC 3 cut(s) 1187, 1802, 1952
AspS9I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
AsuC2I CCSGG 4 cut(s) 288, 1306, 1872, 1873
AsuHPI GGTGA 3 cut(s) 19, 143, 2014
AsuNHI GCTAGC 1 cut(s) 1964
AvaI CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
AvaII GGWCC 3 cut(s) 1517, 1590, 2078
BaeGI GKGCMC 1 cut(s) 565
BalI TGGCCA 1 cut(s) 938
BanI GGYRCC 1 cut(s) 1310
BanII GRGCYC 3 cut(s) 1724, 1878, 2037
BauI CACGAG 1 cut(s) 2103
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 3 cut(s) 565, 748, 2037
BbvCI CCTCAGC 1 cut(s) 1794
BcgI CGANNNNNNTGC 2 cut(s) 1541, 1575
BciT130I CCWGG 3 cut(s) 15, 1070, 1566
BciVI GTATCC 1 cut(s) 2268
BcnI CCSGG 4 cut(s) 288, 1306, 1872, 1873
BcoDI GTCTC 6 cut(s) 153, 456, 474, 1322, 1534, 2312
BfaI CTAG 3 cut(s) 336, 869, 1965
BfmI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
BfoI RGCGCY 2 cut(s) 1188, 1803
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 1 cut(s) 2268
BlpI GCTNAGC 1 cut(s) 802
Bme1390I CCNGG 7 cut(s) 15, 288, 1070, 1306, 1566, 1872, 1873
Bme18I GGWCC 3 cut(s) 1517, 1590, 2078
BmeT110I CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
BmiI GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
BmrFI CCNGG 7 cut(s) 15, 288, 1070, 1306, 1566, 1872, 1873
BmrI ACTGGG 1 cut(s) 2257
BmsI GCATC 5 cut(s) 634, 899, 1159, 1169, 1977
BmtI GCTAGC 1 cut(s) 1968
BmuI ACTGGG 1 cut(s) 2257
BoxI GACNNNNGTC 1 cut(s) 1991
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 2 cut(s) 2263, 2295
BpmI CTGGAG 3 cut(s) 399, 1611, 2201
Bpu10I CCTNAGC 2 cut(s) 132, 1794
Bpu1102I GCTNAGC 1 cut(s) 802
BpuEI CTTGAG 2 cut(s) 246, 2021
BpuMI CCSGG 4 cut(s) 288, 1306, 1872, 1873
BsaI GGTCTC 1 cut(s) 1322
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 136
Bse118I RCCGGY 3 cut(s) 683, 1313, 2120
Bse1I ACTGG 6 cut(s) 382, 439, 793, 1628, 2081, 2252
Bse3DI GCAATG 3 cut(s) 538, 1155, 2097
BseBI CCWGG 3 cut(s) 15, 1070, 1566
BseGI GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BseLI CCNNNNNNNGG 2 cut(s) 14, 136
BseMI GCAATG 3 cut(s) 538, 1155, 2097
BseMII CTCAG 8 cut(s) 123, 135, 816, 963, 1343, 1785, 1820, 1911
BseNI ACTGG 6 cut(s) 382, 439, 793, 1628, 2081, 2252
BseRI GAGGAG 3 cut(s) 406, 753, 1794
BseSI GKGCMC 1 cut(s) 565
BseYI CCCAGC 2 cut(s) 1187, 1337
BsgI GTGCAG 1 cut(s) 1266
BshFI GGCC 3 cut(s) 445, 938, 978
BshNI GGYRCC 1 cut(s) 1310
BsiHKAI GWGCWC 3 cut(s) 565, 748, 2037
BsiHKCI CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
BsiSI CCGG 6 cut(s) 288, 684, 1305, 1314, 1872, 2121
BslFI GGGAC 4 cut(s) 729, 1488, 1576, 2187
BslI CCNNNNNNNGG 2 cut(s) 14, 136
BsmAI GTCTC 6 cut(s) 153, 456, 474, 1322, 1534, 2312
BsmFI GGGAC 4 cut(s) 729, 1488, 1576, 2187
BsmI GAATGC 1 cut(s) 1499
BsnI GGCC 3 cut(s) 445, 938, 978
Bso31I GGTCTC 1 cut(s) 1322
BsoBI CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
Bsp1286I GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 4 cut(s) 993, 1259, 1768, 1819
Bsp1720I GCTNAGC 1 cut(s) 802
Bsp19I CCATGG 2 cut(s) 1659, 1923
BspACI CCGC 1 cut(s) 878
BspANI GGCC 3 cut(s) 445, 938, 978
BspCNI CTCAG 8 cut(s) 124, 136, 815, 962, 1342, 1786, 1819, 1910
BspHI TCATGA 1 cut(s) 2029
BspLI GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
BspMAI CTGCAG 5 cut(s) 54, 247, 682, 800, 1729
BspMI ACCTGC 1 cut(s) 2125
BspOI GCTAGC 1 cut(s) 1968
BspPI GGATC 1 cut(s) 1814
BspQI GCTCTTC 1 cut(s) 1698
BspT107I GGYRCC 1 cut(s) 1310
BspTNI GGTCTC 1 cut(s) 1322
BsrDI GCAATG 3 cut(s) 538, 1155, 2097
BsrFI RCCGGY 3 cut(s) 683, 1313, 2120
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 6 cut(s) 382, 439, 793, 1628, 2081, 2252
BssAI RCCGGY 3 cut(s) 683, 1313, 2120
BssMI GATC 4 cut(s) 993, 1259, 1768, 1819
BssNAI GTATAC 1 cut(s) 1107
BssSI CACGAG 1 cut(s) 2103
BssT1I CCWWGG 3 cut(s) 1010, 1659, 1923
Bst1107I GTATAC 1 cut(s) 1107
Bst2BI CACGAG 1 cut(s) 2103
Bst2UI CCWGG 3 cut(s) 15, 1070, 1566
Bst4CI ACNGT 8 cut(s) 1024, 1087, 1294, 1374, 2053, 2065, 2287, 2296
Bst6I CTCTTC 1 cut(s) 1698
BstAPI GCANNNNNTGC 1 cut(s) 1974
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 4 cut(s) 565, 706, 1033, 1966
BstDEI CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
BstDSI CCRYGG 2 cut(s) 1659, 1923
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BstH2I RGCGCY 2 cut(s) 1188, 1803
BstHHI GCGC 3 cut(s) 1187, 1802, 1952
BstKTI GATC 4 cut(s) 996, 1262, 1771, 1822
BstMAI GTCTC 6 cut(s) 153, 456, 474, 1322, 1534, 2312
BstMBI GATC 4 cut(s) 993, 1259, 1768, 1819
BstMWI GCNNNNNNNGC 5 cut(s) 1191, 1310, 1799, 1949, 1974
BstNI CCWGG 3 cut(s) 15, 1070, 1566
BstNSI RCATGY 2 cut(s) 1847, 1977
BstPAI GACNNNNGTC 1 cut(s) 1991
BstSCI CCNGG 7 cut(s) 13, 286, 1068, 1304, 1564, 1870, 1871
BstSFI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
BstSLI GKGCMC 1 cut(s) 565
BstV2I GAAGAC 1 cut(s) 1185
BstXI CCANNNNNNTGG 2 cut(s) 495, 756
BstZ17I GTATAC 1 cut(s) 1107
BsuI GTATCC 1 cut(s) 2268
BsuRI GGCC 3 cut(s) 445, 938, 978
BtgI CCRYGG 2 cut(s) 1659, 1923
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BtsI GCAGTG 2 cut(s) 675, 1275
BtsIMutI CAGTG 5 cut(s) 432, 675, 1020, 1275, 2292
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 4 cut(s) 565, 706, 1033, 1966
CciI TCATGA 1 cut(s) 2029
CfoI GCGC 3 cut(s) 1187, 1802, 1952
Cfr10I RCCGGY 3 cut(s) 683, 1313, 2120
Cfr13I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
Cfr9I CCCGGG 1 cut(s) 1871
CseI GACGC 1 cut(s) 839
Csp6I GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
CviQI GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
DdeI CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
DpnI GATC 4 cut(s) 995, 1261, 1770, 1821
DpnII GATC 4 cut(s) 993, 1259, 1768, 1819
DraI TTTAAA 1 cut(s) 1639
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 1698
EarI CTCTTC 1 cut(s) 1698
EciI GGCGGA 1 cut(s) 867
Ecl136II GAGCTC 1 cut(s) 2035
Eco130I CCWWGG 3 cut(s) 1010, 1659, 1923
Eco24I GRGCYC 3 cut(s) 1724, 1878, 2037
Eco31I GGTCTC 1 cut(s) 1322
Eco47I GGWCC 3 cut(s) 1517, 1590, 2078
Eco47III AGCGCT 1 cut(s) 1186
Eco53kI GAGCTC 1 cut(s) 2035
Eco57I CTGAAG 3 cut(s) 633, 708, 1565
Eco88I CYCGRG 5 cut(s) 446, 639, 1360, 1512, 1871
EcoICRI GAGCTC 1 cut(s) 2035
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 1517
EcoRII CCWGG 3 cut(s) 13, 1068, 1564
EcoT14I CCWWGG 3 cut(s) 1010, 1659, 1923
EcoT38I GRGCYC 3 cut(s) 1724, 1878, 2037
ErhI CCWWGG 3 cut(s) 1010, 1659, 1923
FalI AAGNNNNNCTT 2 cut(s) 2215, 2247
FaqI GGGAC 4 cut(s) 729, 1488, 1576, 2187
FauNDI CATATG 2 cut(s) 169, 506
FblI GTMKAC 3 cut(s) 360, 1106, 1369
FokI GGATG 9 cut(s) 658, 917, 1126, 1130, 1258, 1358, 1691, 1892, 2059
FriOI GRGCYC 3 cut(s) 1724, 1878, 2037
FspBI CTAG 3 cut(s) 336, 869, 1965
GlaI GCGC 3 cut(s) 1186, 1801, 1951
GsaI CCCAGC 2 cut(s) 1191, 1341
GsuI CTGGAG 3 cut(s) 399, 1611, 2201
HaeII RGCGCY 2 cut(s) 1188, 1803
HaeIII GGCC 3 cut(s) 445, 938, 978
HapII CCGG 6 cut(s) 288, 684, 1305, 1314, 1872, 2121
HgaI GACGC 1 cut(s) 839
HhaI GCGC 3 cut(s) 1187, 1802, 1952
Hin6I GCGC 3 cut(s) 1185, 1800, 1950
HinP1I GCGC 3 cut(s) 1185, 1800, 1950
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 2 cut(s) 457, 706
HpaII CCGG 6 cut(s) 288, 684, 1305, 1314, 1872, 2121
HphI GGTGA 3 cut(s) 19, 143, 2014
Hpy188I TCNGA 3 cut(s) 145, 1809, 1900
Hpy188III TCNNGA 9 cut(s) 320, 512, 651, 726, 1203, 1257, 1650, 2030, 2058
HpyAV CCTTC 5 cut(s) 77, 509, 821, 1545, 2025
HpyCH4III ACNGT 8 cut(s) 1024, 1087, 1294, 1374, 2053, 2065, 2287, 2296
HpyCH4IV ACGT 1 cut(s) 852
HpyF10VI GCNNNNNNNGC 5 cut(s) 1191, 1310, 1799, 1949, 1974
HpyF3I CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
HpySE526I ACGT 1 cut(s) 852
HspAI GCGC 3 cut(s) 1185, 1800, 1950
Kzo9I GATC 4 cut(s) 993, 1259, 1768, 1819
LguI GCTCTTC 1 cut(s) 1698
LweI GCATC 5 cut(s) 634, 899, 1159, 1169, 1977
MaeI CTAG 3 cut(s) 336, 869, 1965
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 2 cut(s) 25, 2065
MalI GATC 4 cut(s) 995, 1261, 1770, 1821
MboI GATC 4 cut(s) 993, 1259, 1768, 1819
MboII GAAGA 9 cut(s) 32, 562, 1190, 1495, 1685, 1778, 1822, 2235, 2264
MhlI GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
MlsI TGGCCA 1 cut(s) 938
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 6 cut(s) 155, 356, 403, 432, 458, 1208
MmeI TCCRAC 2 cut(s) 323, 476
Mox20I TGGCCA 1 cut(s) 938
MroXI GAANNNNTTC 3 cut(s) 204, 1127, 2227
MscI TGGCCA 1 cut(s) 938
MseI TTAA 7 cut(s) 1451, 1638, 1698, 2001, 2085, 2133, 2139
Msp20I TGGCCA 1 cut(s) 938
MspI CCGG 6 cut(s) 288, 684, 1305, 1314, 1872, 2121
MspR9I CCNGG 7 cut(s) 15, 288, 1070, 1306, 1566, 1872, 1873
Mva1269I GAATGC 1 cut(s) 1499
MvaI CCWGG 3 cut(s) 15, 1070, 1566
MwoI GCNNNNNNNGC 5 cut(s) 1191, 1310, 1799, 1949, 1974
NciI CCSGG 4 cut(s) 288, 1306, 1872, 1873
NcoI CCATGG 2 cut(s) 1659, 1923
NdeI CATATG 2 cut(s) 169, 506
NdeII GATC 4 cut(s) 993, 1259, 1768, 1819
NheI GCTAGC 1 cut(s) 1964
NlaIV GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
NmuCI GTSAC 2 cut(s) 25, 2065
NspI RCATGY 2 cut(s) 1847, 1977
PaeR7I CTCGAG 1 cut(s) 1512
PagI TCATGA 1 cut(s) 2029
PaqCI CACCTGC 1 cut(s) 2125
PasI CCCWGGG 1 cut(s) 1069
PciI ACATGT 1 cut(s) 1843
PciSI GCTCTTC 1 cut(s) 1698
PctI GAATGC 1 cut(s) 1499
PdmI GAANNNNTTC 3 cut(s) 204, 1127, 2227
PfeI GAWTC 4 cut(s) 160, 200, 945, 1646
PleI GAGTC 6 cut(s) 154, 356, 402, 431, 457, 1207
PpsI GAGTC 6 cut(s) 154, 356, 402, 431, 457, 1207
PpuMI RGGWCCY 1 cut(s) 1517
PscI ACATGT 1 cut(s) 1843
PshAI GACNNNNGTC 1 cut(s) 1991
Psp124BI GAGCTC 1 cut(s) 2037
Psp5II RGGWCCY 1 cut(s) 1517
Psp6I CCWGG 3 cut(s) 13, 1068, 1564
PspFI CCCAGC 2 cut(s) 1187, 1337
PspGI CCWGG 3 cut(s) 13, 1068, 1564
PspN4I GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
PspPI GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
PspPPI RGGWCCY 1 cut(s) 1517
PspXI VCTCGAGB 1 cut(s) 1512
PsrI GAACNNNNNNTAC 2 cut(s) 1115, 1147
PstI CTGCAG 5 cut(s) 54, 247, 682, 800, 1729
RsaI GTAC 6 cut(s) 212, 285, 661, 1219, 1542, 2246
RsaNI GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
SacI GAGCTC 1 cut(s) 2037
SapI GCTCTTC 1 cut(s) 1698
SaqAI TTAA 7 cut(s) 1451, 1638, 1698, 2001, 2085, 2133, 2139
Sau3AI GATC 4 cut(s) 993, 1259, 1768, 1819
Sau96I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
SchI GAGTC 6 cut(s) 155, 356, 403, 432, 458, 1208
ScrFI CCNGG 7 cut(s) 15, 288, 1070, 1306, 1566, 1872, 1873
SduI GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
SfaNI GCATC 5 cut(s) 634, 899, 1159, 1169, 1977
SfcI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
Sfr274I CTCGAG 1 cut(s) 1512
SinI GGWCC 3 cut(s) 1517, 1590, 2078
SlaI CTCGAG 1 cut(s) 1512
SmaI CCCGGG 1 cut(s) 1873
SmlI CTYRAG 3 cut(s) 261, 1512, 2036
SmoI CTYRAG 3 cut(s) 261, 1512, 2036
SsiI CCGC 1 cut(s) 878
SspMI CTAG 3 cut(s) 336, 869, 1965
SstI GAGCTC 1 cut(s) 2037
StyD4I CCNGG 7 cut(s) 13, 286, 1068, 1304, 1564, 1870, 1871
StyI CCWWGG 3 cut(s) 1010, 1659, 1923
TaaI ACNGT 8 cut(s) 1024, 1087, 1294, 1374, 2053, 2065, 2287, 2296
TaiI ACGT 1 cut(s) 855
TaqI TCGA 7 cut(s) 389, 1041, 1258, 1513, 1788, 1827, 1985
TatI WGTACW 2 cut(s) 210, 2244
TfiI GAWTC 4 cut(s) 160, 200, 945, 1646
Tru1I TTAA 7 cut(s) 1451, 1638, 1698, 2001, 2085, 2133, 2139
Tru9I TTAA 7 cut(s) 1451, 1638, 1698, 2001, 2085, 2133, 2139
TscAI CASTG 5 cut(s) 439, 682, 1027, 1282, 2292
TseFI GTSAC 2 cut(s) 25, 2065
Tsp45I GTSAC 2 cut(s) 25, 2065
TspDTI ATGAA 5 cut(s) 600, 917, 1191, 2018, 2252
TspGWI ACGGA 1 cut(s) 1898
TspMI CCCGGG 1 cut(s) 1871
TspRI CASTG 5 cut(s) 439, 682, 1027, 1282, 2292
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 3 cut(s) 1517, 1590, 2078
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 4 cut(s) 438, 593, 626, 1000
XceI RCATGY 2 cut(s) 1847, 1977
XhoI CTCGAG 1 cut(s) 1512
XmaI CCCGGG 1 cut(s) 1871
XmiI GTMKAC 3 cut(s) 360, 1106, 1369
XmnI GAANNNNTTC 3 cut(s) 204, 1127, 2227
XspI CTAG 3 cut(s) 336, 869, 1965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.