Rh3DG207300

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001662
Physical Location & Seq
Forward (+)
18937362 .. 18939278
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG207300.1

Sequence Viewer

Length: 741 bp
ATGTCATCTCCGGCGGTTTTAATCAAGATGCCGGTGGAGTCTAGATTCAGGCTTGAGAGGCCGTTTGACATCGCGGAGGCCTTTTCTCAAAGGCGATCTGGCTCGCTTGCGGTTCTAAACGATCAGCGCGTGGAGTCGTTGGAATCGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCCGCAGGAGGTGATGGGCTCGCGCGGAGCAGAATGAGGACACCGAGAGCCGCGATTGGTCGAGAGAATGTGCTGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTGTGTTGCCTTCTTGGTATCCAAGAACCCCTCTCCGTGATATAACTGCGATTACCAGGGCCATTGAGAGGAGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGGTCAATTTCCATCTCTACAAGTTCAGGATGCTCCTCCTGAGCATCAAGTTCTTGCTGCTACTCCTTCAACCAATAAGAAGAGACCTTGCCCACCTCCTTCTGTTCTTAAAGTGCACAAAATTCTGCTAGAGGTGGCTAATCAGCCTGATGAGGGAGAGTTCGTTACACCACAGAAGAAGCTGCTGAACTCGATTGACAAAGTTGAGAAAGTAGTGTTGGAAGAACTTCAGAGGCAGAAGAGGACGCCAAGTGCTAAGAAGGCAGAGAGGGAAAGAAAGGTTCGAACTTTGATGTCAATGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.6

Weight (kDa)

11.22

Isoelectric Point (pI)

74.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 74, 129, 227, 229, 257
AciI CCGC 7 cut(s) 14, 74, 110, 157, 207, 229, 255
AclWI GGATC 1 cut(s) 322
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 680
AfiI CCNNNNNNNGG 3 cut(s) 304, 390, 587
AgeI ACCGGT 1 cut(s) 175
AgsI TTSAA 1 cut(s) 504
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AluBI AGCT 2 cut(s) 401, 616
AluI AGCT 2 cut(s) 401, 616
Alw21I GWGCWC 2 cut(s) 403, 552
Alw26I GTCTC 2 cut(s) 181, 511
Alw44I GTGCAC 1 cut(s) 548
AlwI GGATC 1 cut(s) 322
AoxI GGCC 5 cut(s) 59, 78, 204, 282, 381
ApaLI GTGCAC 1 cut(s) 548
ApeKI GCWGC 3 cut(s) 277, 491, 616
ApoI RAATTY 1 cut(s) 555
AsiGI ACCGGT 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 2 cut(s) 129, 229
AspS9I GGNCC 2 cut(s) 204, 381
AsuHPI GGTGA 2 cut(s) 227, 422
AsuII TTCGAA 1 cut(s) 718
BaeGI GKGCMC 1 cut(s) 552
BanII GRGCYC 2 cut(s) 225, 403
Bbv12I GWGCWC 2 cut(s) 403, 552
BbvI GCAGC 3 cut(s) 264, 478, 603
BccI CCATC 2 cut(s) 212, 454
BceAI ACGGC 2 cut(s) 46, 206
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 379
BciVI GTATCC 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 181, 511
BfaI CTAG 4 cut(s) 42, 194, 294, 563
BfmI CTRYAG 1 cut(s) 429
BfuI GTATCC 1 cut(s) 351
BglI GCCNNNNNGGC 1 cut(s) 154
BisI GCNGC 5 cut(s) 207, 255, 278, 492, 617
BlsI GCNGC 5 cut(s) 208, 256, 279, 493, 618
Bme1390I CCNGG 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 204, 381
BmrFI CCNGG 1 cut(s) 379
BmsI GCATC 4 cut(s) 18, 190, 454, 487
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu10I CCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 1 cut(s) 378
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 390, 587
Bse118I RCCGGY 3 cut(s) 31, 148, 175
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 3 cut(s) 304, 390, 587
BseMII CTCAG 1 cut(s) 465
BseRI GAGGAG 3 cut(s) 406, 428, 459
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 3 cut(s) 264, 478, 603
Bsh1236I CGCG 5 cut(s) 74, 129, 227, 229, 257
BshFI GGCC 5 cut(s) 61, 80, 206, 284, 383
BshTI ACCGGT 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 403, 552
BsiSI CCGG 6 cut(s) 11, 32, 149, 176, 285, 298
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 3 cut(s) 304, 390, 587
BsmAI GTCTC 2 cut(s) 181, 511
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 5 cut(s) 61, 80, 206, 284, 383
Bso31I GGTCTC 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 3 cut(s) 225, 403, 552
Bsp143I GATC 3 cut(s) 95, 121, 314
BspACI CCGC 7 cut(s) 14, 74, 110, 157, 207, 229, 255
BspANI GGCC 5 cut(s) 61, 80, 206, 284, 383
BspCNI CTCAG 1 cut(s) 466
BspFNI CGCG 5 cut(s) 74, 129, 227, 229, 257
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 1 cut(s) 322
BspQI GCTCTTC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 718
BspTNI GGTCTC 1 cut(s) 511
BsrFI RCCGGY 3 cut(s) 31, 148, 175
BssAI RCCGGY 3 cut(s) 31, 148, 175
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 3 cut(s) 95, 121, 314
BssNI GRCGYC 1 cut(s) 680
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 3 cut(s) 391, 509, 668
BstACI GRCGYC 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 6 cut(s) 104, 108, 150, 225, 282, 403
BstDEI CTNAG 2 cut(s) 474, 690
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 5 cut(s) 74, 129, 227, 229, 257
BstHHI GCGC 2 cut(s) 129, 229
BstKTI GATC 3 cut(s) 98, 124, 317
BstMAI GTCTC 2 cut(s) 181, 511
BstMBI GATC 3 cut(s) 95, 121, 314
BstMWI GCNNNNNNNGC 3 cut(s) 58, 154, 695
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 5 cut(s) 74, 129, 227, 229, 257
BstV1I GCAGC 3 cut(s) 264, 478, 603
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 5 cut(s) 61, 80, 206, 284, 383
BtgZI GCGATG 1 cut(s) 55
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 6 cut(s) 104, 108, 150, 225, 282, 403
CfoI GCGC 2 cut(s) 129, 229
Cfr10I RCCGGY 3 cut(s) 31, 148, 175
Cfr13I GGNCC 2 cut(s) 204, 381
CseI GACGC 1 cut(s) 688
CspAI ACCGGT 1 cut(s) 175
DdeI CTNAG 2 cut(s) 474, 690
DpnI GATC 3 cut(s) 97, 123, 316
DpnII GATC 3 cut(s) 95, 121, 314
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 3 cut(s) 391, 509, 668
EarI CTCTTC 3 cut(s) 391, 509, 668
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 401
Eco147I AGGCCT 1 cut(s) 80
Eco24I GRGCYC 2 cut(s) 225, 403
Eco31I GGTCTC 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 377
EcoT38I GRGCYC 2 cut(s) 225, 403
Esp3I CGTCTC 1 cut(s) 181
FaiI YATR 2 cut(s) 202, 365
FaqI GGGAC 1 cut(s) 321
Fnu4HI GCNGC 5 cut(s) 207, 255, 278, 492, 617
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 2 cut(s) 225, 403
Fsp4HI GCNGC 5 cut(s) 207, 255, 278, 492, 617
FspBI CTAG 4 cut(s) 42, 194, 294, 563
GlaI GCGC 2 cut(s) 128, 228
GluI GCNGC 5 cut(s) 207, 255, 278, 492, 617
HaeIII GGCC 5 cut(s) 61, 80, 206, 284, 383
HapII CCGG 6 cut(s) 11, 32, 149, 176, 285, 298
HgaI GACGC 1 cut(s) 688
HhaI GCGC 2 cut(s) 129, 229
Hin1I GRCGYC 1 cut(s) 680
Hin6I GCGC 2 cut(s) 127, 227
HinP1I GCGC 2 cut(s) 127, 227
HinfI GANTC 4 cut(s) 38, 45, 134, 143
HpaII CCGG 6 cut(s) 11, 32, 149, 176, 285, 298
HphI GGTGA 2 cut(s) 227, 422
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 2 cut(s) 186, 666
Hpy188III TCNNGA 5 cut(s) 25, 42, 266, 461, 473
Hpy8I GTNNAC 1 cut(s) 550
HpyAV CCTTC 5 cut(s) 297, 342, 510, 543, 688
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4V TGCA 3 cut(s) 181, 431, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 154, 695
HpyF3I CTNAG 2 cut(s) 474, 690
Hsp92I GRCGYC 1 cut(s) 680
HspAI GCGC 2 cut(s) 127, 227
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 95, 121, 314
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 2 cut(s) 231, 472
Lsp1109I GCAGC 3 cut(s) 264, 478, 603
LweI GCATC 4 cut(s) 18, 190, 454, 487
MaeI CTAG 4 cut(s) 42, 194, 294, 563
MaeIII GTNAC 1 cut(s) 598
MalI GATC 3 cut(s) 97, 123, 316
MboI GATC 3 cut(s) 95, 121, 314
MboII GAAGA 5 cut(s) 408, 526, 622, 668, 685
MhlI GDGCHC 3 cut(s) 225, 403, 552
MluCI AATT 2 cut(s) 440, 555
MlyI GAGTC 2 cut(s) 47, 143
MmeI TCCRAC 2 cut(s) 120, 633
MreI CGCCGGCG 1 cut(s) 148
MroNI GCCGGC 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 2 cut(s) 20, 543
MspI CCGG 6 cut(s) 11, 32, 149, 176, 285, 298
MspR9I CCNGG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 379
MvnI CGCG 5 cut(s) 74, 129, 227, 229, 257
MwoI GCNNNNNNNGC 3 cut(s) 58, 154, 695
NaeI GCCGGC 1 cut(s) 150
NdeII GATC 3 cut(s) 95, 121, 314
NgoMIV GCCGGC 1 cut(s) 148
NspV TTCGAA 1 cut(s) 718
PceI AGGCCT 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 45, 143
PinAI ACCGGT 1 cut(s) 175
PkrI GCNGC 5 cut(s) 208, 256, 279, 493, 618
PleI GAGTC 2 cut(s) 46, 142
PpsI GAGTC 2 cut(s) 46, 142
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspPI GGNCC 2 cut(s) 204, 381
PstI CTGCAG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 2 cut(s) 20, 543
SatI GCNGC 5 cut(s) 207, 255, 278, 492, 617
Sau3AI GATC 3 cut(s) 95, 121, 314
Sau96I GGNCC 2 cut(s) 204, 381
SchI GAGTC 2 cut(s) 47, 143
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 3 cut(s) 225, 403, 552
SetI ASST 7 cut(s) 217, 403, 523, 532, 570, 618, 717
SfaNI GCATC 4 cut(s) 18, 190, 454, 487
SfcI CTRYAG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 718
SgrAI CRCCGGYG 2 cut(s) 148, 175
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 2 cut(s) 440, 555
SseBI AGGCCT 1 cut(s) 80
SsiI CCGC 7 cut(s) 14, 74, 110, 157, 207, 229, 255
SspMI CTAG 4 cut(s) 42, 194, 294, 563
SstI GAGCTC 1 cut(s) 403
StuI AGGCCT 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 377
TaaI ACNGT 1 cut(s) 323
TaqI TCGA 4 cut(s) 265, 317, 626, 718
TasI AATT 2 cut(s) 440, 555
TauI GCSGC 2 cut(s) 209, 257
TfiI GAWTC 2 cut(s) 45, 143
Tru1I TTAA 2 cut(s) 20, 543
Tru9I TTAA 2 cut(s) 20, 543
TscAI CASTG 1 cut(s) 328
TseI GCWGC 3 cut(s) 277, 491, 616
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 548
XapI RAATTY 1 cut(s) 555
XbaI TCTAGA 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 4 cut(s) 42, 194, 294, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.