RLG00000002349

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001662
Physical Location & Seq
Reverse (-)
32592636 .. 32595143
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002349

Sequence Viewer

Length: 714 bp
ATGCCGGTGGAGTCTAGATTCAGGCTTGAGAGGCCGTTTGACGTTGCGGAGGCCTTTTCTCAAAGGCGATCTGGCTCGCTTGCTGTTCTAATCGATCAGCGCGTGGAGTCGTTGGAATCGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCCGCAGGAGGTGATGGGCTTGCAAGGAGCAGAATGAGGACACCGAGAGCCGCGATTGGTCAAGAGATTGTGGTGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTATGTTGCCTTCTTGGTATCCAAGAACCCCTCTCCGTGATATAACTGCGATTACCAGGGCCATTGAGAGGAGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGATCAATTTCCATCTCTACAAGTTCAGGATGCTTCTCCTGAGCATCAAGTTCTCGCTGCTACTCCTTCAACCATTAAGAAGAGACCTTGCCCACCTAGTTCTGTTCTTAAAGTGCACAAAATTCTGCTAGAGGTGGCTAATCAGCCTGATGAGGGAGAGTTCGTTACACCACAGAAGAAGCTACTGAACTCAATTGACAAAGTTGAGAAGGTGGTGTTGGAAAAACTTCAGAGGCTGAAGAGGACCCCGAGTGCTAAGAAGGCAGAGAGGGAAAGAAAGGTTCGAACTTTGATGTCAATGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.67

Weight (kDa)

11.14

Isoelectric Point (pI)

69.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 102, 230
AciI CCGC 4 cut(s) 47, 130, 180, 228
AclWI GGATC 2 cut(s) 295, 417
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 620, 665
AfiI CCNNNNNNNGG 3 cut(s) 277, 363, 560
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 1 cut(s) 477
AjnI CCWGG 1 cut(s) 350
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 2 cut(s) 374, 589
AluI AGCT 2 cut(s) 374, 589
Alw21I GWGCWC 2 cut(s) 376, 525
Alw26I GTCTC 2 cut(s) 154, 484
Alw44I GTGCAC 1 cut(s) 521
AlwI GGATC 2 cut(s) 295, 417
AlwNI CAGNNNCTG 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 655
AoxI GGCC 5 cut(s) 32, 51, 177, 255, 354
ApaLI GTGCAC 1 cut(s) 521
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 1 cut(s) 528
AsiGI ACCGGT 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 3 cut(s) 177, 354, 651
AsuHPI GGTGA 2 cut(s) 200, 395
AsuII TTCGAA 1 cut(s) 691
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 525
BanII GRGCYC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
Bbv12I GWGCWC 2 cut(s) 376, 525
BbvI GCAGC 1 cut(s) 451
BccI CCATC 2 cut(s) 185, 427
BceAI ACGGC 2 cut(s) 19, 179
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 352
BciVI GTATCC 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 154, 484
BfaI CTAG 5 cut(s) 15, 167, 267, 504, 536
BfmI CTRYAG 1 cut(s) 402
BfuI GTATCC 1 cut(s) 324
BglI GCCNNNNNGGC 1 cut(s) 127
BisI GCNGC 3 cut(s) 180, 228, 465
BlsI GCNGC 3 cut(s) 181, 229, 466
Bme1390I CCNGG 1 cut(s) 352
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 1 cut(s) 655
BmgT120I GGNCC 3 cut(s) 177, 354, 651
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 1 cut(s) 352
BmsI GCATC 3 cut(s) 163, 427, 460
BplI GAGNNNNNCTC 2 cut(s) 358, 390
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 691
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 363, 560
Bse118I RCCGGY 3 cut(s) 4, 121, 148
BseBI CCWGG 1 cut(s) 352
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 3 cut(s) 277, 363, 560
BseMII CTCAG 1 cut(s) 438
BseRI GAGGAG 2 cut(s) 379, 401
BseSI GKGCMC 1 cut(s) 525
BseXI GCAGC 1 cut(s) 451
Bsh1236I CGCG 2 cut(s) 102, 230
BshFI GGCC 5 cut(s) 34, 53, 179, 257, 356
BshTI ACCGGT 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 2 cut(s) 376, 525
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 5 cut(s) 5, 122, 149, 258, 271
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 3 cut(s) 277, 363, 560
BsmAI GTCTC 2 cut(s) 154, 484
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 5 cut(s) 34, 53, 179, 257, 356
Bso31I GGTCTC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 655
Bsp119I TTCGAA 1 cut(s) 691
Bsp1286I GDGCHC 2 cut(s) 376, 525
Bsp143I GATC 4 cut(s) 68, 94, 287, 409
BspACI CCGC 4 cut(s) 47, 130, 180, 228
BspANI GGCC 5 cut(s) 34, 53, 179, 257, 356
BspCNI CTCAG 1 cut(s) 439
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 2 cut(s) 102, 230
BspLI GGNNCC 1 cut(s) 653
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 2 cut(s) 295, 417
BspQI GCTCTTC 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 691
BspTNI GGTCTC 1 cut(s) 484
BsrFI RCCGGY 3 cut(s) 4, 121, 148
BssAI RCCGGY 3 cut(s) 4, 121, 148
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 4 cut(s) 68, 94, 287, 409
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 3 cut(s) 364, 482, 641
BstBI TTCGAA 1 cut(s) 691
BstC8I GCNNGC 6 cut(s) 77, 81, 123, 198, 255, 376
BstDEI CTNAG 2 cut(s) 447, 663
BstF5I GGATG 1 cut(s) 442
BstFNI CGCG 2 cut(s) 102, 230
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 71, 97, 290, 412
BstMAI GTCTC 2 cut(s) 154, 484
BstMBI GATC 4 cut(s) 68, 94, 287, 409
BstMWI GCNNNNNNNGC 3 cut(s) 31, 127, 668
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 2 cut(s) 102, 230
BstV1I GCAGC 1 cut(s) 451
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 5 cut(s) 34, 53, 179, 257, 356
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 442
Cac8I GCNNGC 6 cut(s) 77, 81, 123, 198, 255, 376
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 3 cut(s) 4, 121, 148
Cfr13I GGNCC 3 cut(s) 177, 354, 651
ClaI ATCGAT 1 cut(s) 93
CspAI ACCGGT 1 cut(s) 148
DdeI CTNAG 2 cut(s) 447, 663
DpnI GATC 4 cut(s) 70, 96, 289, 411
DpnII GATC 4 cut(s) 68, 94, 287, 409
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 364, 482, 641
EarI CTCTTC 3 cut(s) 364, 482, 641
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 374
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 620, 665
Eco88I CYCGRG 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 376
Esp3I CGTCTC 1 cut(s) 154
FaiI YATR 3 cut(s) 175, 299, 338
FaqI GGGAC 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 180, 228, 465
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 3 cut(s) 180, 228, 465
FspBI CTAG 5 cut(s) 15, 167, 267, 504, 536
GlaI GCGC 1 cut(s) 101
GluI GCNGC 3 cut(s) 180, 228, 465
HaeIII GGCC 5 cut(s) 34, 53, 179, 257, 356
HapII CCGG 5 cut(s) 5, 122, 149, 258, 271
HhaI GCGC 1 cut(s) 102
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 4 cut(s) 11, 18, 107, 116
HpaII CCGG 5 cut(s) 5, 122, 149, 258, 271
HphI GGTGA 2 cut(s) 200, 395
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 2 cut(s) 159, 639
Hpy188III TCNNGA 4 cut(s) 15, 239, 434, 446
Hpy8I GTNNAC 1 cut(s) 523
HpyAV CCTTC 5 cut(s) 270, 315, 483, 610, 661
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 154, 200, 404, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 127, 668
HpyF3I CTNAG 2 cut(s) 447, 663
HpySE526I ACGT 1 cut(s) 42
HspAI GCGC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 4 cut(s) 68, 94, 287, 409
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 3 cut(s) 163, 427, 460
MaeI CTAG 5 cut(s) 15, 167, 267, 504, 536
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 571
MalI GATC 4 cut(s) 70, 96, 289, 411
MboI GATC 4 cut(s) 68, 94, 287, 409
MboII GAAGA 4 cut(s) 381, 499, 595, 658
MfeI CAATTG 1 cut(s) 600
MhlI GDGCHC 2 cut(s) 376, 525
MluCI AATT 3 cut(s) 413, 528, 600
MlyI GAGTC 2 cut(s) 20, 116
MmeI TCCRAC 2 cut(s) 93, 606
MreI CGCCGGCG 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 2 cut(s) 483, 516
MspI CCGG 5 cut(s) 5, 122, 149, 258, 271
MspR9I CCNGG 1 cut(s) 352
MunI CAATTG 1 cut(s) 600
MvaI CCWGG 1 cut(s) 352
MvnI CGCG 2 cut(s) 102, 230
MwoI GCNNNNNNNGC 3 cut(s) 31, 127, 668
NaeI GCCGGC 1 cut(s) 123
NdeII GATC 4 cut(s) 68, 94, 287, 409
NgoMIV GCCGGC 1 cut(s) 121
NlaIV GGNNCC 1 cut(s) 653
NspV TTCGAA 1 cut(s) 691
PceI AGGCCT 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 18, 116
PinAI ACCGGT 1 cut(s) 148
PkrI GCNGC 3 cut(s) 181, 229, 466
PleI GAGTC 2 cut(s) 19, 115
PpsI GAGTC 2 cut(s) 19, 115
PpuMI RGGWCCY 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 376
Psp5II RGGWCCY 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 3 cut(s) 177, 354, 651
PspPPI RGGWCCY 1 cut(s) 651
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 643
SacI GAGCTC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 364
SaqAI TTAA 2 cut(s) 483, 516
SatI GCNGC 3 cut(s) 180, 228, 465
Sau3AI GATC 4 cut(s) 68, 94, 287, 409
Sau96I GGNCC 3 cut(s) 177, 354, 651
SchI GAGTC 2 cut(s) 20, 116
ScrFI CCNGG 1 cut(s) 352
SduI GDGCHC 2 cut(s) 376, 525
SetI ASST 9 cut(s) 45, 190, 376, 496, 505, 543, 591, 621, 690
SfaNI GCATC 3 cut(s) 163, 427, 460
SfcI CTRYAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 691
SgrAI CRCCGGYG 2 cut(s) 121, 148
SinI GGWCC 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 3 cut(s) 413, 528, 600
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 4 cut(s) 47, 130, 180, 228
SspMI CTAG 5 cut(s) 15, 167, 267, 504, 536
SstI GAGCTC 1 cut(s) 376
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 93, 290, 691
TasI AATT 3 cut(s) 413, 528, 600
TauI GCSGC 2 cut(s) 182, 230
TfiI GAWTC 2 cut(s) 18, 116
Tru1I TTAA 2 cut(s) 483, 516
Tru9I TTAA 2 cut(s) 483, 516
TseI GCWGC 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 320
VneI GTGCAC 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 1 cut(s) 528
XbaI TCTAGA 1 cut(s) 14
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 5 cut(s) 15, 167, 267, 504, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.