Rh7AG326200

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001662
Physical Location & Seq
Forward (+)
37216730 .. 37219530
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG326200.1

Sequence Viewer

Length: 798 bp
ATGCCGGTGGAGTCTAGATTCAGGCTTGAGAGGCCGTTTGACGTTGCGGAGGCCTTTTCTCAAAGGCGATCTGGCTCGCTTGCTGTTCTAATCGATCAGCGCGTGGAGTCGTTGGAATTGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCCGTAGGAGGTATGAGGACACCGAGAGCCGCGATTGGTCAACAGAATGTGCTGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTGTGTTGCCTTCTTGGTATCCAAGAACCCCTCTCTGTGATATAACTGCTATTATCAGAGGAGTCCATTGGATTACCATGTTTCAGTTGGGTTTAATTGGTTTGTTGAACAAAGAGTCTCATTGGATTCATCTCATGGTCTTGGGTGTATTATTGGCTTTATTCGCCATTGAGAGGAGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGGTCAATTTCCATCTCTACAAGTTCAGGATGCTTCTCCTGAGCATCAAGTTCTCGCTGCTACTCCTTCAACCATTAAGAAGAGACCTTGCCCACCTAGTTCTGTTCTTAAAGTGCACAAAATTCTTCTAGAGGTGGCTAATCAGCCTGATGAGGGAGAGTTTGTTACACCACAGAAGAAGCTACTGAACTCAATTGACAAAGTTGAGAAGGTGGTGTTGGAAGAACTTCAGAGGCTGAAGAGGACCCCGAGTGCTAAGAAGGCAGAGAGGGAAAGAAAGGTTCGAACTTTGATGTCAATGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

29.83

Weight (kDa)

10.82

Isoelectric Point (pI)

60.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 102, 209
AciI CCGC 3 cut(s) 47, 130, 207
AclWI GGATC 1 cut(s) 274
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 2 cut(s) 704, 749
AfiI CCNNNNNNNGG 3 cut(s) 256, 447, 644
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 2 cut(s) 382, 561
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 2 cut(s) 458, 673
AluI AGCT 2 cut(s) 458, 673
Alw21I GWGCWC 2 cut(s) 460, 609
Alw26I GTCTC 3 cut(s) 154, 396, 568
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 1 cut(s) 274
AlwNI CAGNNNCTG 1 cut(s) 727
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 4 cut(s) 32, 51, 177, 234
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 2 cut(s) 229, 548
ApoI RAATTY 1 cut(s) 612
AsiGI ACCGGT 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 177, 735
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 775
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 1 cut(s) 735
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 460
Bbv12I GWGCWC 2 cut(s) 460, 609
BbvI GCAGC 2 cut(s) 216, 535
BccI CCATC 1 cut(s) 511
BceAI ACGGC 3 cut(s) 19, 164, 179
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciVI GTATCC 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 154, 396, 568
BfaI CTAG 5 cut(s) 15, 167, 246, 588, 620
BfmI CTRYAG 1 cut(s) 486
BfuI GTATCC 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 127
BisI GCNGC 3 cut(s) 207, 230, 549
BlsI GCNGC 3 cut(s) 208, 231, 550
Bme18I GGWCC 1 cut(s) 735
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 177, 735
BmiI GGNNCC 1 cut(s) 737
BmsI GCATC 3 cut(s) 163, 511, 544
BplI GAGNNNNNCTC 2 cut(s) 442, 474
Bpu10I CCTNAGC 1 cut(s) 531
Bpu14I TTCGAA 1 cut(s) 775
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 568
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 447, 644
Bse118I RCCGGY 3 cut(s) 4, 121, 148
BseCI ATCGAT 1 cut(s) 93
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 3 cut(s) 256, 447, 644
BseMII CTCAG 1 cut(s) 522
BseRI GAGGAG 3 cut(s) 348, 463, 485
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 2 cut(s) 216, 535
Bsh1236I CGCG 2 cut(s) 102, 209
BshFI GGCC 4 cut(s) 34, 53, 179, 236
BshTI ACCGGT 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 2 cut(s) 460, 609
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 5 cut(s) 5, 122, 149, 237, 250
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 3 cut(s) 256, 447, 644
BsmAI GTCTC 3 cut(s) 154, 396, 568
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 4 cut(s) 34, 53, 179, 236
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 739
Bsp119I TTCGAA 1 cut(s) 775
Bsp1286I GDGCHC 2 cut(s) 460, 609
Bsp143I GATC 3 cut(s) 68, 94, 266
BspACI CCGC 3 cut(s) 47, 130, 207
BspANI GGCC 4 cut(s) 34, 53, 179, 236
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 2 cut(s) 102, 209
BspLI GGNNCC 1 cut(s) 737
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 1 cut(s) 274
BspQI GCTCTTC 1 cut(s) 448
BspT104I TTCGAA 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 568
BsrFI RCCGGY 3 cut(s) 4, 121, 148
BssAI RCCGGY 3 cut(s) 4, 121, 148
BssMI GATC 3 cut(s) 68, 94, 266
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 3 cut(s) 448, 566, 725
BstBI TTCGAA 1 cut(s) 775
BstC8I GCNNGC 5 cut(s) 77, 81, 123, 234, 460
BstDEI CTNAG 2 cut(s) 531, 747
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 2 cut(s) 102, 209
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 71, 97, 269
BstMAI GTCTC 3 cut(s) 154, 396, 568
BstMBI GATC 3 cut(s) 68, 94, 266
BstMWI GCNNNNNNNGC 4 cut(s) 31, 127, 437, 752
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 2 cut(s) 102, 209
BstV1I GCAGC 2 cut(s) 216, 535
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 4 cut(s) 34, 53, 179, 236
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 280
Cac8I GCNNGC 5 cut(s) 77, 81, 123, 234, 460
CaiI CAGNNNCTG 1 cut(s) 727
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 3 cut(s) 4, 121, 148
Cfr13I GGNCC 2 cut(s) 177, 735
ClaI ATCGAT 1 cut(s) 93
CspAI ACCGGT 1 cut(s) 148
CviAII CATG 2 cut(s) 352, 409
DdeI CTNAG 2 cut(s) 531, 747
DpnI GATC 3 cut(s) 70, 96, 268
DpnII GATC 3 cut(s) 68, 94, 266
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 3 cut(s) 448, 566, 725
EarI CTCTTC 3 cut(s) 448, 566, 725
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 458
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 735
Eco53kI GAGCTC 1 cut(s) 458
Eco57I CTGAAG 2 cut(s) 704, 749
Eco88I CYCGRG 1 cut(s) 739
EcoICRI GAGCTC 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 2 cut(s) 355, 412
FaiI YATR 5 cut(s) 175, 191, 317, 353, 410
FaqI GGGAC 1 cut(s) 273
FatI CATG 2 cut(s) 351, 408
Fnu4HI GCNGC 3 cut(s) 207, 230, 549
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 3 cut(s) 207, 230, 549
FspBI CTAG 5 cut(s) 15, 167, 246, 588, 620
GlaI GCGC 1 cut(s) 101
GluI GCNGC 3 cut(s) 207, 230, 549
HaeIII GGCC 4 cut(s) 34, 53, 179, 236
HapII CCGG 5 cut(s) 5, 122, 149, 237, 250
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 355, 412
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 218
HindII GTYRAC 1 cut(s) 218
HinfI GANTC 6 cut(s) 11, 18, 107, 336, 389, 400
HpaII CCGG 5 cut(s) 5, 122, 149, 237, 250
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 2 cut(s) 218, 607
Hpy188I TCNGA 3 cut(s) 159, 332, 723
Hpy188III TCNNGA 4 cut(s) 15, 518, 530, 620
Hpy8I GTNNAC 2 cut(s) 218, 607
HpyAV CCTTC 5 cut(s) 249, 294, 567, 694, 745
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 3 cut(s) 154, 488, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 127, 437, 752
HpyF3I CTNAG 2 cut(s) 531, 747
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 355, 412
HspAI GCGC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 3 cut(s) 68, 94, 266
LguI GCTCTTC 1 cut(s) 448
Lsp1109I GCAGC 2 cut(s) 216, 535
LweI GCATC 3 cut(s) 163, 511, 544
MaeI CTAG 5 cut(s) 15, 167, 246, 588, 620
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 655
MalI GATC 3 cut(s) 70, 96, 268
MboI GATC 3 cut(s) 68, 94, 266
MboII GAAGA 6 cut(s) 465, 583, 608, 679, 725, 742
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 2 cut(s) 460, 609
MluCI AATT 5 cut(s) 116, 369, 497, 612, 684
MlyI GAGTC 4 cut(s) 20, 116, 345, 398
MmeI TCCRAC 2 cut(s) 93, 690
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 3 cut(s) 368, 567, 600
MspI CCGG 5 cut(s) 5, 122, 149, 237, 250
MunI CAATTG 1 cut(s) 684
MvnI CGCG 2 cut(s) 102, 209
MwoI GCNNNNNNNGC 4 cut(s) 31, 127, 437, 752
NaeI GCCGGC 1 cut(s) 123
NdeII GATC 3 cut(s) 68, 94, 266
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 355, 412
NlaIV GGNNCC 1 cut(s) 737
NspV TTCGAA 1 cut(s) 775
PceI AGGCCT 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 18, 400
PinAI ACCGGT 1 cut(s) 148
PkrI GCNGC 3 cut(s) 208, 231, 550
PleI GAGTC 4 cut(s) 19, 115, 344, 397
PpsI GAGTC 4 cut(s) 19, 115, 344, 397
PpuMI RGGWCCY 1 cut(s) 735
Psp124BI GAGCTC 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 735
PspN4I GGNNCC 1 cut(s) 737
PspPI GGNCC 2 cut(s) 177, 735
PspPPI RGGWCCY 1 cut(s) 735
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 727
SacI GAGCTC 1 cut(s) 460
SapI GCTCTTC 1 cut(s) 448
SaqAI TTAA 3 cut(s) 368, 567, 600
SatI GCNGC 3 cut(s) 207, 230, 549
Sau3AI GATC 3 cut(s) 68, 94, 266
Sau96I GGNCC 2 cut(s) 177, 735
SchI GAGTC 4 cut(s) 20, 116, 345, 398
SduI GDGCHC 2 cut(s) 460, 609
SetI ASST 9 cut(s) 45, 190, 460, 580, 589, 627, 675, 705, 774
SfaNI GCATC 3 cut(s) 163, 511, 544
SfcI CTRYAG 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 775
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 735
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 5 cut(s) 116, 369, 497, 612, 684
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 3 cut(s) 47, 130, 207
SspMI CTAG 5 cut(s) 15, 167, 246, 588, 620
SstI GAGCTC 1 cut(s) 460
StuI AGGCCT 1 cut(s) 53
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 93, 269, 775
TasI AATT 5 cut(s) 116, 369, 497, 612, 684
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 2 cut(s) 18, 400
Tru1I TTAA 3 cut(s) 368, 567, 600
Tru9I TTAA 3 cut(s) 368, 567, 600
TscAI CASTG 1 cut(s) 280
TseI GCWGC 2 cut(s) 229, 548
TspDTI ATGAA 1 cut(s) 392
TspRI CASTG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 735
XapI RAATTY 1 cut(s) 612
XbaI TCTAGA 2 cut(s) 14, 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 5 cut(s) 15, 167, 246, 588, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.