RLG00000002322

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001662
Physical Location & Seq
Reverse (-)
32169806 .. 32176691
6886 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002322

Sequence Viewer

Length: 1842 bp
ATGCTGGTGGAGTCTAGATTCAGGCTTGAAAGGCCGCTTGACATCGCGGAGGCCTTTTCTCAAAGGCGATCTGGCTCGCTTGCGGTTCTAATCGATCAGCGCGTGGAGTCGTTGGAATCGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCTACAGGAGGTGATGGGCTCGCAAGGAGCAGAATGAGGACACCGAGAGCCGCGATTGGTCAAGAGAATGTGCTGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTGTGTTGCCTTCTTGGTATCCAAGAACCCCTCTCCGTGATATAACTGCGATTATCAGGGCCATTGAGAGGGGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGGTCAATTTCCATCTCTACAAGTTCAGGATGCTCCTCCTGAGCATCAAGTTCTCGCTGCTACTCCCTCAACCAATAAGAAGAGACCTTGCCCACCTCCTTCTGTTCTTAAAGTGCACAAAATTCTGCTAGAGGTGGCTAATCAGCCTGATGACGGAGAGTTCGTTACACCACAGAAGAAGCTGCTGAACTCGATTGACAAAGTAGAGAAGGTGGTGTTGGAAGAACTTCAGAGGCTGAAGAGGACCCCGAGTGCTAAGAAGGCCGAGAGGGAAAGAAAGAGAGCACGCCGTGGGAGACCGCGCCGTTACTTCCAAGGAGGGCCCCATGCTCCCCATTCCCGGCCAACGCAACGTCCTCATCACCAGCGCACTGCCGTACGTCAACAACGTCCCTCATCGGCTGCGTTCTCAGCGCCGACGTCTTCGCTCGCTACTGCCGTCTCCGGGGATACAACACCGTCTATATCTGCGGCACCGCCACCGAGACCAAAGCCCTGGAGGACAAGTGTAGCCCCAAGGAGATCTGCGATAAGTAAGTCTTGGCAGGTGTATGAGTGGTTCAATATTAGTTTTGACAAATTCGGGCGAACTTCGACGCCGGAGCAGACTGAGGTGTGCCAAGCGATTTTCAATAGGCTGCTGGAGAATAATTGGCTTTCTGAGAACACAATGCAGCAGCTATATTGTGATACTTGCACACGGTTTTTAGCTGACAGGCTCGTGGAGGGGACGTGCCCAACTCAAGGTTGTGATTATACCTCTGCAAGAGGAGATCAGTATGAGAACTGTGGGAAGCTTTTAAACCCCATAGAATTAAAAGATCTGAAGTGCAAGTGGGGGGTTCCAGTTCCACATGATATCTTGAGGGACAAGGTTAATACATGTTTGCCAACTTCCACTGATTATTTTTTAGATTTTCATACAGTAGAGTTTCTGGCATCAGGGAGGATACATGCAAATGAGTTTGATGTGTTGGATTGTAGCAGTGGAAGACTAAAGAAAACCATTTGGAAGCTCTTAAATGCCCCTGATGGTGAGCGGAATTCCGTATCTTCGAGCCACATTGAAGAAGGTATAAAATCAAGCAGTGCCCATTGCATCTCATACTTAATCAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTAAGACAGAAACTCAGGATGCGGGGACTACGCTGCCATCCAATGTACTGCAGAAGCTCAACGCGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCTCTCAGAGCACTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCCACTGCTCTGAAGCAAGTCACCAAATCTACTTCTCTGGAATGTGATTACGTGATGCGACTTGAATGTGGATTTATGTACCTTTTGAGAAGAAACGAGGCCTCCATCAAGAATAAAGGCGGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

614

Amino Acids

68.78

Weight (kDa)

9.94

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 317 - 414 3.8e-28 tRNA synthetases class I (M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 943
AatII GACGTC 1 cut(s) 830
Acc36I ACCTGC 1 cut(s) 943
AccB1I GGYRCC 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 1447
AccII CGCG 5 cut(s) 47, 102, 230, 709, 1618
AclWI GGATC 3 cut(s) 295, 1520, 1533
AcoI YGGCCR 2 cut(s) 255, 749
AcsI RAATTY 3 cut(s) 528, 986, 1450
AcuI CTGAAG 4 cut(s) 620, 665, 1253, 1745
AcyI GRCGYC 2 cut(s) 827, 1004
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 3 cut(s) 786, 1601, 1793
AfiI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
AflIII ACRYGT 1 cut(s) 1289
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 5 cut(s) 29, 970, 1039, 1475, 1778
AjiI CACGTC 1 cut(s) 1140
AjnI CCWGG 1 cut(s) 902
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 9 cut(s) 374, 589, 1087, 1118, 1204, 1423, 1611, 1694, 1703
AluI AGCT 9 cut(s) 374, 589, 1087, 1118, 1204, 1423, 1611, 1694, 1703
Alw21I GWGCWC 5 cut(s) 376, 525, 694, 1654, 1669
Alw26I GTCTC 5 cut(s) 154, 484, 697, 853, 886
Alw44I GTGCAC 1 cut(s) 521
AlwI GGATC 3 cut(s) 295, 1520, 1533
AlwNI CAGNNNCTG 1 cut(s) 643
Ama87I CYCGRG 2 cut(s) 655, 1647
AoxI GGCC 8 cut(s) 32, 51, 255, 354, 669, 728, 749, 1812
ApaI GGGCCC 1 cut(s) 732
ApaLI GTGCAC 1 cut(s) 521
ApeKI GCWGC 8 cut(s) 250, 464, 589, 809, 1045, 1081, 1084, 1587
ApoI RAATTY 3 cut(s) 528, 986, 1450
ArsI GACNNNNNNTTYG 2 cut(s) 551, 583
AsiGI ACCGGT 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 4 cut(s) 102, 711, 777, 823
AspS9I GGNCC 4 cut(s) 354, 651, 728, 729
AsuC2I CCSGG 2 cut(s) 748, 853
AsuHPI GGTGA 5 cut(s) 200, 395, 761, 1454, 1726
AvaI CYCGRG 2 cut(s) 655, 1647
AvaII GGWCC 1 cut(s) 651
BaeGI GKGCMC 4 cut(s) 525, 732, 1145, 1501
BamHI GGATCC 1 cut(s) 1525
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 3 cut(s) 198, 376, 732
BauI CACGAG 1 cut(s) 1127
BbsI GAAGAC 2 cut(s) 822, 1405
Bbv12I GWGCWC 5 cut(s) 376, 525, 694, 1654, 1669
BbvI GCAGC 8 cut(s) 237, 451, 576, 796, 1032, 1093, 1096, 1574
BccI CCATC 5 cut(s) 185, 427, 1433, 1599, 1826
BceAI ACGGC 5 cut(s) 179, 681, 696, 767, 830
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 3 cut(s) 324, 850, 1350
BcnI CCSGG 2 cut(s) 748, 853
BcoDI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BfaI CTAG 5 cut(s) 15, 167, 267, 536, 1530
BfmI CTRYAG 3 cut(s) 180, 402, 1603
BfoI RGCGCY 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 943
BfuI GTATCC 3 cut(s) 324, 850, 1350
BglI GCCNNNNNGGC 1 cut(s) 127
BglII AGATCT 2 cut(s) 929, 1228
Bme1390I CCNGG 3 cut(s) 748, 853, 904
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 2 cut(s) 655, 1647
BmgBI CACGTC 1 cut(s) 1140
BmgT120I GGNCC 4 cut(s) 354, 651, 728, 729
BmiI GGNNCC 6 cut(s) 653, 730, 731, 882, 1251, 1527
BmrFI CCNGG 3 cut(s) 748, 853, 904
BmsI GCATC 7 cut(s) 163, 427, 460, 1355, 1515, 1563, 1758
BpiI GAAGAC 2 cut(s) 822, 1405
BpmI CTGGAG 2 cut(s) 925, 1070
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 2 cut(s) 1134, 1291
BpuMI CCSGG 2 cut(s) 748, 853
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 1765
BsaHI GRCGYC 2 cut(s) 827, 1004
BsaI GGTCTC 3 cut(s) 484, 697, 886
BsaJI CCNNGG 5 cut(s) 697, 721, 852, 902, 923
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 8 cut(s) 555, 585, 898, 928, 1124, 1154, 1799, 1829
Bsc4I CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
Bse118I RCCGGY 2 cut(s) 121, 148
Bse1I ACTGG 2 cut(s) 1253, 1674
Bse3DI GCAATG 1 cut(s) 1501
BseBI CCWGG 1 cut(s) 904
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 5 cut(s) 697, 721, 852, 902, 923
BseGI GGATG 3 cut(s) 442, 1578, 1591
BseLI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
BseMI GCAATG 1 cut(s) 1501
BseMII CTCAG 6 cut(s) 438, 831, 1008, 1059, 1582, 1675
BseNI ACTGG 2 cut(s) 1253, 1674
BseRI GAGGAG 3 cut(s) 401, 432, 1191
BseSI GKGCMC 4 cut(s) 525, 732, 1145, 1501
BseXI GCAGC 8 cut(s) 237, 451, 576, 796, 1032, 1093, 1096, 1574
Bsh1236I CGCG 5 cut(s) 47, 102, 230, 709, 1618
BshFI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1814
BshNI GGYRCC 1 cut(s) 880
BshTI ACCGGT 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 5 cut(s) 376, 525, 694, 1654, 1669
BsiHKCI CYCGRG 2 cut(s) 655, 1647
BsiSI CCGG 7 cut(s) 122, 149, 258, 271, 748, 852, 1007
BsiWI CGTACG 1 cut(s) 784
BslFI GGGAC 5 cut(s) 294, 783, 1150, 1289, 1593
BslI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
BsmAI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BsmBI CGTCTC 2 cut(s) 154, 853
BsmFI GGGAC 5 cut(s) 294, 783, 1150, 1289, 1593
BsmI GAATGC 1 cut(s) 1626
BsnI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1814
Bso31I GGTCTC 3 cut(s) 484, 697, 886
BsoBI CYCGRG 2 cut(s) 655, 1647
Bsp120I GGGCCC 1 cut(s) 728
Bsp1286I GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1501, 1654, 1669
Bsp143I GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
BspANI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1814
BspCNI CTCAG 6 cut(s) 439, 830, 1009, 1060, 1581, 1674
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 5 cut(s) 47, 102, 230, 709, 1618
BspLI GGNNCC 6 cut(s) 653, 730, 731, 882, 1251, 1527
BspMAI CTGCAG 2 cut(s) 406, 1607
BspMI ACCTGC 1 cut(s) 943
BspPI GGATC 3 cut(s) 295, 1520, 1533
BspQI GCTCTTC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 880
BspTNI GGTCTC 3 cut(s) 484, 697, 886
BsrBI CCGCTC 1 cut(s) 1447
BsrDI GCAATG 1 cut(s) 1501
BsrFI RCCGGY 2 cut(s) 121, 148
BsrI ACTGG 2 cut(s) 1253, 1674
BssAI RCCGGY 2 cut(s) 121, 148
BssECI CCNNGG 5 cut(s) 697, 721, 852, 902, 923
BssMI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
BssNI GRCGYC 2 cut(s) 827, 1004
BssSI CACGAG 1 cut(s) 1127
BssT1I CCWWGG 2 cut(s) 721, 923
Bst2BI CACGAG 1 cut(s) 1127
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 5 cut(s) 296, 867, 1110, 1196, 1333
Bst6I CTCTTC 3 cut(s) 364, 482, 641
BstACI GRCGYC 2 cut(s) 827, 1004
BstBAI YACGTR 1 cut(s) 1765
BstC8I GCNNGC 9 cut(s) 77, 81, 123, 198, 255, 376, 694, 837, 1692
BstDEI CTNAG 7 cut(s) 447, 663, 817, 1017, 1068, 1568, 1661
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 3 cut(s) 442, 1578, 1591
BstFNI CGCG 5 cut(s) 47, 102, 230, 709, 1618
BstH2I RGCGCY 1 cut(s) 824
BstHHI GCGC 4 cut(s) 102, 711, 777, 823
BstKTI GATC 7 cut(s) 71, 97, 290, 932, 1183, 1231, 1528
BstMAI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BstMBI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
BstMWI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1649, 1664, 1673, 1700
BstNI CCWGG 1 cut(s) 904
BstNSI RCATGY 2 cut(s) 1293, 1364
BstSCI CCNGG 3 cut(s) 746, 851, 902
BstSFI CTRYAG 3 cut(s) 180, 402, 1603
BstSLI GKGCMC 4 cut(s) 525, 732, 1145, 1501
BstUI CGCG 5 cut(s) 47, 102, 230, 709, 1618
BstV1I GCAGC 8 cut(s) 237, 451, 576, 796, 1032, 1093, 1096, 1574
BstV2I GAAGAC 2 cut(s) 822, 1405
BstX2I RGATCY 3 cut(s) 929, 1228, 1525
BstXI CCANNNNNNTGG 1 cut(s) 903
BstYI RGATCY 3 cut(s) 929, 1228, 1525
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 3 cut(s) 324, 850, 1350
BsuRI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1814
BsuTUI ATCGAT 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 697
BtgZI GCGATG 1 cut(s) 28
BtrI CACGTC 1 cut(s) 1140
BtsCI GGATG 3 cut(s) 442, 1578, 1591
BtsI GCAGTG 4 cut(s) 777, 1399, 1501, 1716
BtsIMutI CAGTG 7 cut(s) 301, 777, 1305, 1399, 1501, 1667, 1716
BveI ACCTGC 1 cut(s) 943
Cac8I GCNNGC 9 cut(s) 77, 81, 123, 198, 255, 376, 694, 837, 1692
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 4 cut(s) 102, 711, 777, 823
Cfr10I RCCGGY 2 cut(s) 121, 148
Cfr13I GGNCC 4 cut(s) 354, 651, 728, 729
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 1012
Csp6I GTAC 3 cut(s) 785, 1600, 1792
CspAI ACCGGT 1 cut(s) 148
CviAII CATG 4 cut(s) 734, 1262, 1290, 1361
CviQI GTAC 3 cut(s) 785, 1600, 1792
DdeI CTNAG 7 cut(s) 447, 663, 817, 1017, 1068, 1568, 1661
DpnI GATC 7 cut(s) 70, 96, 289, 931, 1182, 1230, 1527
DpnII GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
DraI TTTAAA 1 cut(s) 1209
DraIII CACNNNGTG 1 cut(s) 698
EaeI YGGCCR 2 cut(s) 255, 749
Eam1104I CTCTTC 3 cut(s) 364, 482, 641
EarI CTCTTC 3 cut(s) 364, 482, 641
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 721, 923
Eco147I AGGCCT 2 cut(s) 53, 1814
Eco24I GRGCYC 3 cut(s) 198, 376, 732
Eco31I GGTCTC 3 cut(s) 484, 697, 886
Eco32I GATATC 1 cut(s) 1267
Eco47I GGWCC 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 4 cut(s) 620, 665, 1253, 1745
Eco88I CYCGRG 2 cut(s) 655, 1647
EcoICRI GAGCTC 1 cut(s) 374
EcoO109I RGGNCCY 3 cut(s) 651, 728, 729
EcoRI GAATTC 1 cut(s) 1450
EcoRII CCWGG 1 cut(s) 902
EcoRV GATATC 1 cut(s) 1267
EcoT14I CCWWGG 2 cut(s) 721, 923
EcoT38I GRGCYC 3 cut(s) 198, 376, 732
ErhI CCWWGG 2 cut(s) 721, 923
Esp3I CGTCTC 2 cut(s) 154, 853
FaeI CATG 4 cut(s) 737, 1265, 1293, 1364
FalI AAGNNNNNCTT 2 cut(s) 931, 963
FaqI GGGAC 5 cut(s) 294, 783, 1150, 1289, 1593
FatI CATG 4 cut(s) 733, 1261, 1289, 1360
FauI CCCGC 1 cut(s) 1569
FokI GGATG 3 cut(s) 449, 1578, 1585
FriOI GRGCYC 3 cut(s) 198, 376, 732
FspBI CTAG 5 cut(s) 15, 167, 267, 536, 1530
GlaI GCGC 4 cut(s) 101, 710, 776, 822
GsuI CTGGAG 2 cut(s) 925, 1070
HaeII RGCGCY 1 cut(s) 824
HaeIII GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1814
HapII CCGG 7 cut(s) 122, 149, 258, 271, 748, 852, 1007
HgaI GACGC 1 cut(s) 1012
HhaI GCGC 4 cut(s) 102, 711, 777, 823
Hin1I GRCGYC 2 cut(s) 827, 1004
Hin1II CATG 4 cut(s) 737, 1265, 1293, 1364
Hin6I GCGC 4 cut(s) 100, 709, 775, 821
HinP1I GCGC 4 cut(s) 100, 709, 775, 821
HincII GTYRAC 1 cut(s) 791
HindII GTYRAC 1 cut(s) 791
HindIII AAGCTT 1 cut(s) 1202
HinfI GANTC 4 cut(s) 11, 18, 107, 116
HpaII CCGG 7 cut(s) 122, 149, 258, 271, 748, 852, 1007
HphI GGTGA 5 cut(s) 200, 395, 761, 1454, 1726
Hpy166II GTNNAC 2 cut(s) 523, 791
Hpy188I TCNGA 6 cut(s) 159, 639, 1069, 1233, 1664, 1725
Hpy188III TCNNGA 9 cut(s) 15, 239, 434, 446, 1270, 1570, 1647, 1751, 1822
Hpy8I GTNNAC 2 cut(s) 523, 791
Hpy99I CGWCG 2 cut(s) 829, 1006
HpyAV CCTTC 6 cut(s) 270, 315, 516, 610, 661, 1472
HpyCH4III ACNGT 5 cut(s) 296, 867, 1110, 1196, 1333
HpyCH4IV ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1764
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1649, 1664, 1673, 1700
HpyF3I CTNAG 7 cut(s) 447, 663, 817, 1017, 1068, 1568, 1661
HpySE526I ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1764
Hsp92I GRCGYC 2 cut(s) 827, 1004
Hsp92II CATG 4 cut(s) 737, 1265, 1293, 1364
HspAI GCGC 4 cut(s) 100, 709, 775, 821
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 5 cut(s) 204, 445, 742, 1009, 1699
Lsp1109I GCAGC 8 cut(s) 237, 451, 576, 796, 1032, 1093, 1096, 1574
LweI GCATC 7 cut(s) 163, 427, 460, 1355, 1515, 1563, 1758
MaeI CTAG 5 cut(s) 15, 167, 267, 536, 1530
MaeII ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1764
MaeIII GTNAC 3 cut(s) 571, 713, 1732
MalI GATC 7 cut(s) 70, 96, 289, 931, 1182, 1230, 1527
MbiI CCGCTC 1 cut(s) 1447
MboI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
MflI RGATCY 3 cut(s) 929, 1228, 1525
MhlI GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1501, 1654, 1669
MluCI AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1450, 1626
MlyI GAGTC 2 cut(s) 20, 116
MmeI TCCRAC 4 cut(s) 93, 606, 1362, 1721
MreI CGCCGGCG 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 7 cut(s) 516, 1208, 1223, 1284, 1427, 1517, 1556
MslI CAYNNNNRTG 1 cut(s) 1365
MspA1I CMGCKG 1 cut(s) 1703
MspI CCGG 7 cut(s) 122, 149, 258, 271, 748, 852, 1007
MspR9I CCNGG 3 cut(s) 748, 853, 904
Mva1269I GAATGC 1 cut(s) 1626
MvaI CCWGG 1 cut(s) 904
MvnI CGCG 5 cut(s) 47, 102, 230, 709, 1618
MwoI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1649, 1664, 1673, 1700
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 2 cut(s) 748, 853
NdeII GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 4 cut(s) 737, 1265, 1293, 1364
NlaIV GGNNCC 6 cut(s) 653, 730, 731, 882, 1251, 1527
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 1732
NspI RCATGY 2 cut(s) 1293, 1364
PaeR7I CTCGAG 1 cut(s) 1647
PaqCI CACCTGC 1 cut(s) 943
PceI AGGCCT 2 cut(s) 53, 1814
PciI ACATGT 1 cut(s) 1289
PciSI GCTCTTC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 567, 793, 843
PctI GAATGC 1 cut(s) 1626
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 18, 116
Pfl23II CGTACG 1 cut(s) 784
PinAI ACCGGT 1 cut(s) 148
PleI GAGTC 2 cut(s) 19, 115
PpsI GAGTC 2 cut(s) 19, 115
Ppu21I YACGTR 1 cut(s) 1765
PpuMI RGGWCCY 1 cut(s) 651
PscI ACATGT 1 cut(s) 1289
Psp124BI GAGCTC 1 cut(s) 376
Psp5II RGGWCCY 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 902
PspGI CCWGG 1 cut(s) 902
PspLI CGTACG 1 cut(s) 784
PspN4I GGNNCC 6 cut(s) 653, 730, 731, 882, 1251, 1527
PspOMI GGGCCC 1 cut(s) 728
PspPI GGNCC 4 cut(s) 354, 651, 728, 729
PspPPI RGGWCCY 1 cut(s) 651
PsrI GAACNNNNNNTAC 2 cut(s) 950, 982
PstI CTGCAG 2 cut(s) 406, 1607
PstNI CAGNNNCTG 1 cut(s) 643
PsuI RGATCY 3 cut(s) 929, 1228, 1525
PvuII CAGCTG 1 cut(s) 1703
RsaI GTAC 3 cut(s) 786, 1601, 1793
RsaNI GTAC 3 cut(s) 785, 1600, 1792
RseI CAYNNNNRTG 1 cut(s) 1365
SacI GAGCTC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 364
SaqAI TTAA 7 cut(s) 516, 1208, 1223, 1284, 1427, 1517, 1556
Sau3AI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1525
Sau96I GGNCC 4 cut(s) 354, 651, 728, 729
SchI GAGTC 2 cut(s) 20, 116
ScrFI CCNGG 3 cut(s) 748, 853, 904
SduI GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1501, 1654, 1669
SfaNI GCATC 7 cut(s) 163, 427, 460, 1355, 1515, 1563, 1758
SfcI CTRYAG 3 cut(s) 180, 402, 1603
Sfr274I CTCGAG 1 cut(s) 1647
SgrAI CRCCGGYG 2 cut(s) 121, 148
SinI GGWCC 1 cut(s) 651
SlaI CTCGAG 1 cut(s) 1647
SmiMI CAYNNNNRTG 1 cut(s) 1365
SmlI CTYRAG 3 cut(s) 1149, 1270, 1647
SmoI CTYRAG 3 cut(s) 1149, 1270, 1647
Sse9I AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1450, 1626
SseBI AGGCCT 2 cut(s) 53, 1814
SspI AATATT 1 cut(s) 973
SspMI CTAG 5 cut(s) 15, 167, 267, 536, 1530
SstI GAGCTC 1 cut(s) 376
StuI AGGCCT 2 cut(s) 53, 1814
StyD4I CCNGG 3 cut(s) 746, 851, 902
StyI CCWWGG 2 cut(s) 721, 923
TaaI ACNGT 5 cut(s) 296, 867, 1110, 1196, 1333
TaiI ACGT 6 cut(s) 763, 790, 799, 830, 1142, 1767
TaqI TCGA 6 cut(s) 93, 290, 599, 1001, 1463, 1648
TasI AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1450, 1626
TatI WGTACW 1 cut(s) 1599
TauI GCSGC 3 cut(s) 37, 230, 881
TfiI GAWTC 2 cut(s) 18, 116
Tru1I TTAA 7 cut(s) 516, 1208, 1223, 1284, 1427, 1517, 1556
Tru9I TTAA 7 cut(s) 516, 1208, 1223, 1284, 1427, 1517, 1556
TscAI CASTG 7 cut(s) 301, 784, 1312, 1399, 1501, 1674, 1723
TseFI GTSAC 1 cut(s) 1732
TseI GCWGC 8 cut(s) 250, 464, 589, 809, 1045, 1081, 1084, 1587
Tsp45I GTSAC 1 cut(s) 1732
TspDTI ATGAA 1 cut(s) 1316
TspGWI ACGGA 3 cut(s) 320, 576, 1444
TspRI CASTG 7 cut(s) 301, 784, 1312, 1399, 1501, 1674, 1723
VneI GTGCAC 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 3 cut(s) 528, 986, 1450
XbaI TCTAGA 1 cut(s) 14
XceI RCATGY 2 cut(s) 1293, 1364
XhoI CTCGAG 1 cut(s) 1647
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 5 cut(s) 15, 167, 267, 536, 1530
ZraI GACGTC 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.