RLG00000002350

negative regulation of ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001662
Physical Location & Seq
Forward (+)
32634297 .. 32641181
6885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002350

Sequence Viewer

Length: 1779 bp
ATGCCGGTGGAGTCTAGATTCAGGCTTGAAAGGCCGCTTGACATCGCGGAGGCCTTTTCTCAAAGGCGATCTGGCTCGCTTGCGGTTCTAATCGATCAGCGCGTGGAGTCGTTGGAATCGCCGGCGAGGCGGAGGGCGTTAGCGACACCGGTGCATCAGAGACGGCTAGGGTTATGGGCTACAGGAGGTGATGGGCTCGCAAGGAGCAGAATGAGGACACCGAGAGCCGCGATTGGTCAAGAGAATGTGCTGCTGGCCGGAAGTTCTAGCCGGAGAAGGGGACGGGGATCGAACAGTGTGTTGCCTTCTTGGTATCCAAGAACCCCTCTCCGTGATATAACTGCGATTATCAGGGCCATTGAGAGGGGAAGAGCTCGCTTGGGTGAGGAGAATGGCGAAACTGCAGAGGGTCAATTTCCATCTCTACAAGTTCAGGATGCTCCTCCTGAGCATCAAGTTCTCGCTGCTACTCCTTCAACCAATAAGAAGAGACCTTGCCCACCTCCTTCTGTTCTTAAAGTGCACAAAATTCTGCTAGAGGTGGCTAATCAGCCTGATGAGGGAGAGTTCGTTACACCACAGAAGAAGCTGCTGAACTCGATTGACAAAGTAGAGAAGGTGGTGTTGGAAGAACTTCAGAGGCTGAAGAGGACCCCGAGTGCTACGAAGGCCGAGAGGGGAAGAAAGAGAGCACGCCGTGGGAGACCGCGCCGTTACTTCCAAGGAGGGCCCCATGCTCCCCATTCCCGGCCAACGCAACGTCCTCATCACCAGCGCACTGCCGTACGTCAACAACGTCCCTCATCGGCTGCGTTCTCAGCGCCGACGTCTTCGCTCGCTACTGCCGTCTCCGGGGATACAACACCGTCTATATCTGCGGCACCGCCACCGAGACCAAAGCCCTGGAGGACAAGTGTAGCCCCAAGGAGATCTGCGATAAGTAAGTCTTGGCAGCTGTATGAGTGGTTCAATATTAGTTTTGACAAATTCGGGCGAACTTCGACGCCGGAGCAGACTGAGGTGTGCCAAGCGATTTTCAATAGGCTGCTGGAGAATAATTGGCTTTCTGAGAACACAATGCAGCAGCTATATTGTGATACTTGCCCACGGTTTTTAGCTGACAGGCTCGTGGAGGGGACGTGCCCAACTCAAGGTTGTGATTATACCTCTGCAAGAGGAGATCAGTATGAGAACTGTGGGAAGCTTTTAAACCCCATAGAATTAAAAGATCTGAAGTGCAAGGTCTGTAGTTGGGGAGATTTTCATACAGTAGAGTTTCTGGCATCAGGGAGGATACATGCAAATGAGTTTGATGTGTTGGATTGTAGCAGTGGAAGACTAAAGAAAACCATTTGGAAGCTCTTAAATGCCCCTGATGGTGAGCGGAATTCCGTATCTTCGAGCCACATTGAAGAAGGTATAAAATCAAGCAGTGCCCATTGCATCTCATACTTAATCAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTAAGACAGAAACTCAGGATGCGGGGACTACGCTGCCATCCAATGTACTGCAGAAGCTCAACGCGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCTCTCAGAGCACTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCCACTGCTCTGAAGCAAGTCACCAAATCTACTTCTCTGGAATGTGATTACGTGATGCGACTTGAATGTGGATTTATGTACCTTTTGAGAAGAAACGAGGCCTCCATCAAGAATAAAGGCGGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0002831 GO:0002833 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007088 GO:0007113 GO:0007127 GO:0007135 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009553 GO:0009561 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009960 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0010154 GO:0010224 GO:0010564 GO:0010605 GO:0016043 GO:0019222 GO:0022402 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031347 GO:0031349 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032101 GO:0032103 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0034641 GO:0034645 GO:0040020 GO:0042023 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044092 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048468 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051321 GO:0051338 GO:0051348 GO:0051438 GO:0051444 GO:0051445 GO:0051726 GO:0051783 GO:0060255 GO:0061458 GO:0061982 GO:0061983 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0090558 GO:0090626 GO:0140013 GO:1900424 GO:1900426 GO:1901360 GO:1901576 GO:1903046 GO:1903320 GO:1903321 GO:1904666 GO:1904667 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

593

Amino Acids

66.25

Weight (kDa)

10.03

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 317 - 417 5.3e-29 tRNA synthetases class I (M)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 830
AccB1I GGYRCC 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 1384
AccII CGCG 5 cut(s) 47, 102, 230, 709, 1555
AclWI GGATC 3 cut(s) 295, 1457, 1470
AcoI YGGCCR 2 cut(s) 255, 749
AcsI RAATTY 3 cut(s) 528, 986, 1387
AcuI CTGAAG 4 cut(s) 620, 665, 1253, 1682
AcyI GRCGYC 2 cut(s) 827, 1004
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 3 cut(s) 786, 1538, 1730
AfiI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 6 cut(s) 29, 477, 970, 1039, 1412, 1715
AjiI CACGTC 1 cut(s) 1140
AjnI CCWGG 1 cut(s) 902
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
Alw21I GWGCWC 5 cut(s) 376, 525, 694, 1591, 1606
Alw26I GTCTC 5 cut(s) 154, 484, 697, 853, 886
Alw44I GTGCAC 1 cut(s) 521
AlwI GGATC 3 cut(s) 295, 1457, 1470
AlwNI CAGNNNCTG 1 cut(s) 643
Ama87I CYCGRG 2 cut(s) 655, 1584
AoxI GGCC 8 cut(s) 32, 51, 255, 354, 669, 728, 749, 1749
ApaI GGGCCC 1 cut(s) 732
ApaLI GTGCAC 1 cut(s) 521
ApeKI GCWGC 9 cut(s) 250, 464, 589, 809, 952, 1045, 1081, 1084, 1524
ApoI RAATTY 3 cut(s) 528, 986, 1387
AsiGI ACCGGT 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 4 cut(s) 102, 711, 777, 823
AspS9I GGNCC 4 cut(s) 354, 651, 728, 729
AsuC2I CCSGG 2 cut(s) 748, 853
AsuHPI GGTGA 5 cut(s) 200, 395, 761, 1391, 1663
AvaI CYCGRG 2 cut(s) 655, 1584
AvaII GGWCC 1 cut(s) 651
BaeGI GKGCMC 4 cut(s) 525, 732, 1145, 1438
BamHI GGATCC 1 cut(s) 1462
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 3 cut(s) 198, 376, 732
BauI CACGAG 1 cut(s) 1127
BbsI GAAGAC 2 cut(s) 822, 1342
Bbv12I GWGCWC 5 cut(s) 376, 525, 694, 1591, 1606
BbvI GCAGC 9 cut(s) 237, 451, 576, 796, 964, 1032, 1093, 1096, 1511
BccI CCATC 5 cut(s) 185, 427, 1370, 1536, 1763
BceAI ACGGC 5 cut(s) 179, 681, 696, 767, 830
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 3 cut(s) 324, 850, 1287
BcnI CCSGG 2 cut(s) 748, 853
BcoDI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BfaI CTAG 5 cut(s) 15, 167, 267, 536, 1467
BfmI CTRYAG 4 cut(s) 180, 402, 1246, 1540
BfoI RGCGCY 1 cut(s) 824
BfuI GTATCC 3 cut(s) 324, 850, 1287
BglI GCCNNNNNGGC 1 cut(s) 127
BglII AGATCT 2 cut(s) 929, 1228
Bme1390I CCNGG 3 cut(s) 748, 853, 904
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 2 cut(s) 655, 1584
BmgBI CACGTC 1 cut(s) 1140
BmgT120I GGNCC 4 cut(s) 354, 651, 728, 729
BmiI GGNNCC 5 cut(s) 653, 730, 731, 882, 1464
BmrFI CCNGG 3 cut(s) 748, 853, 904
BmsI GCATC 7 cut(s) 163, 427, 460, 1292, 1452, 1500, 1695
BpiI GAAGAC 2 cut(s) 822, 1342
BpmI CTGGAG 2 cut(s) 925, 1070
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 1134
BpuMI CCSGG 2 cut(s) 748, 853
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 1702
BsaHI GRCGYC 2 cut(s) 827, 1004
BsaI GGTCTC 3 cut(s) 484, 697, 886
BsaJI CCNNGG 6 cut(s) 697, 721, 852, 902, 923, 1106
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 8 cut(s) 555, 585, 898, 928, 1124, 1154, 1736, 1766
Bsc4I CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
Bse118I RCCGGY 3 cut(s) 4, 121, 148
Bse1I ACTGG 1 cut(s) 1611
Bse3DI GCAATG 1 cut(s) 1438
BseBI CCWGG 1 cut(s) 904
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 6 cut(s) 697, 721, 852, 902, 923, 1106
BseGI GGATG 3 cut(s) 442, 1515, 1528
BseLI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
BseMI GCAATG 1 cut(s) 1438
BseMII CTCAG 6 cut(s) 438, 831, 1008, 1059, 1519, 1612
BseNI ACTGG 1 cut(s) 1611
BseRI GAGGAG 3 cut(s) 401, 432, 1191
BseSI GKGCMC 4 cut(s) 525, 732, 1145, 1438
BseXI GCAGC 9 cut(s) 237, 451, 576, 796, 964, 1032, 1093, 1096, 1511
Bsh1236I CGCG 5 cut(s) 47, 102, 230, 709, 1555
BshFI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1751
BshNI GGYRCC 1 cut(s) 880
BshTI ACCGGT 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 5 cut(s) 376, 525, 694, 1591, 1606
BsiHKCI CYCGRG 2 cut(s) 655, 1584
BsiSI CCGG 8 cut(s) 5, 122, 149, 258, 271, 748, 852, 1007
BsiWI CGTACG 1 cut(s) 784
BslFI GGGAC 4 cut(s) 294, 783, 1150, 1530
BslI CCNNNNNNNGG 6 cut(s) 277, 363, 560, 747, 852, 1151
BsmAI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BsmBI CGTCTC 2 cut(s) 154, 853
BsmFI GGGAC 4 cut(s) 294, 783, 1150, 1530
BsmI GAATGC 1 cut(s) 1563
BsnI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1751
Bso31I GGTCTC 3 cut(s) 484, 697, 886
BsoBI CYCGRG 2 cut(s) 655, 1584
Bsp120I GGGCCC 1 cut(s) 728
Bsp1286I GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1438, 1591, 1606
Bsp143I GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
BspANI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1751
BspCNI CTCAG 6 cut(s) 439, 830, 1009, 1060, 1518, 1611
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 5 cut(s) 47, 102, 230, 709, 1555
BspLI GGNNCC 5 cut(s) 653, 730, 731, 882, 1464
BspMAI CTGCAG 2 cut(s) 406, 1544
BspPI GGATC 3 cut(s) 295, 1457, 1470
BspQI GCTCTTC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 880
BspTNI GGTCTC 3 cut(s) 484, 697, 886
BsrBI CCGCTC 1 cut(s) 1384
BsrDI GCAATG 1 cut(s) 1438
BsrFI RCCGGY 3 cut(s) 4, 121, 148
BsrI ACTGG 1 cut(s) 1611
BssAI RCCGGY 3 cut(s) 4, 121, 148
BssECI CCNNGG 6 cut(s) 697, 721, 852, 902, 923, 1106
BssMI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
BssNI GRCGYC 2 cut(s) 827, 1004
BssSI CACGAG 1 cut(s) 1127
BssT1I CCWWGG 2 cut(s) 721, 923
Bst2BI CACGAG 1 cut(s) 1127
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 5 cut(s) 296, 867, 1110, 1196, 1270
Bst6I CTCTTC 3 cut(s) 364, 482, 641
BstACI GRCGYC 2 cut(s) 827, 1004
BstBAI YACGTR 1 cut(s) 1702
BstC8I GCNNGC 9 cut(s) 77, 81, 123, 198, 255, 376, 694, 837, 1629
BstDEI CTNAG 6 cut(s) 447, 817, 1017, 1068, 1505, 1598
BstDSI CCRYGG 2 cut(s) 697, 1106
BstF5I GGATG 3 cut(s) 442, 1515, 1528
BstFNI CGCG 5 cut(s) 47, 102, 230, 709, 1555
BstH2I RGCGCY 1 cut(s) 824
BstHHI GCGC 4 cut(s) 102, 711, 777, 823
BstKTI GATC 7 cut(s) 71, 97, 290, 932, 1183, 1231, 1465
BstMAI GTCTC 5 cut(s) 154, 484, 697, 853, 886
BstMBI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
BstMWI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1586, 1601, 1610, 1637
BstNI CCWGG 1 cut(s) 904
BstNSI RCATGY 1 cut(s) 1301
BstSCI CCNGG 3 cut(s) 746, 851, 902
BstSFI CTRYAG 4 cut(s) 180, 402, 1246, 1540
BstSLI GKGCMC 4 cut(s) 525, 732, 1145, 1438
BstUI CGCG 5 cut(s) 47, 102, 230, 709, 1555
BstV1I GCAGC 9 cut(s) 237, 451, 576, 796, 964, 1032, 1093, 1096, 1511
BstV2I GAAGAC 2 cut(s) 822, 1342
BstX2I RGATCY 3 cut(s) 929, 1228, 1462
BstXI CCANNNNNNTGG 1 cut(s) 903
BstYI RGATCY 3 cut(s) 929, 1228, 1462
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 3 cut(s) 324, 850, 1287
BsuRI GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1751
BsuTUI ATCGAT 1 cut(s) 93
BtgI CCRYGG 2 cut(s) 697, 1106
BtgZI GCGATG 1 cut(s) 28
BtrI CACGTC 1 cut(s) 1140
BtsCI GGATG 3 cut(s) 442, 1515, 1528
BtsI GCAGTG 4 cut(s) 777, 1336, 1438, 1653
BtsIMutI CAGTG 6 cut(s) 301, 777, 1336, 1438, 1604, 1653
Cac8I GCNNGC 9 cut(s) 77, 81, 123, 198, 255, 376, 694, 837, 1629
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 4 cut(s) 102, 711, 777, 823
Cfr10I RCCGGY 3 cut(s) 4, 121, 148
Cfr13I GGNCC 4 cut(s) 354, 651, 728, 729
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 1012
Csp6I GTAC 3 cut(s) 785, 1537, 1729
CspAI ACCGGT 1 cut(s) 148
CviAII CATG 2 cut(s) 734, 1298
CviQI GTAC 3 cut(s) 785, 1537, 1729
DdeI CTNAG 6 cut(s) 447, 817, 1017, 1068, 1505, 1598
DpnI GATC 7 cut(s) 70, 96, 289, 931, 1182, 1230, 1464
DpnII GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
DraI TTTAAA 1 cut(s) 1209
DraIII CACNNNGTG 1 cut(s) 698
EaeI YGGCCR 2 cut(s) 255, 749
Eam1104I CTCTTC 3 cut(s) 364, 482, 641
EarI CTCTTC 3 cut(s) 364, 482, 641
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 721, 923
Eco147I AGGCCT 2 cut(s) 53, 1751
Eco24I GRGCYC 3 cut(s) 198, 376, 732
Eco31I GGTCTC 3 cut(s) 484, 697, 886
Eco47I GGWCC 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 4 cut(s) 620, 665, 1253, 1682
Eco88I CYCGRG 2 cut(s) 655, 1584
EcoICRI GAGCTC 1 cut(s) 374
EcoO109I RGGNCCY 3 cut(s) 651, 728, 729
EcoRI GAATTC 1 cut(s) 1387
EcoRII CCWGG 1 cut(s) 902
EcoT14I CCWWGG 2 cut(s) 721, 923
EcoT38I GRGCYC 3 cut(s) 198, 376, 732
ErhI CCWWGG 2 cut(s) 721, 923
Esp3I CGTCTC 2 cut(s) 154, 853
FaeI CATG 2 cut(s) 737, 1301
FalI AAGNNNNNCTT 2 cut(s) 931, 963
FaqI GGGAC 4 cut(s) 294, 783, 1150, 1530
FatI CATG 2 cut(s) 733, 1297
FauI CCCGC 1 cut(s) 1506
FokI GGATG 3 cut(s) 449, 1515, 1522
FriOI GRGCYC 3 cut(s) 198, 376, 732
FspBI CTAG 5 cut(s) 15, 167, 267, 536, 1467
GlaI GCGC 4 cut(s) 101, 710, 776, 822
GsuI CTGGAG 2 cut(s) 925, 1070
HaeII RGCGCY 1 cut(s) 824
HaeIII GGCC 8 cut(s) 34, 53, 257, 356, 671, 730, 751, 1751
HapII CCGG 8 cut(s) 5, 122, 149, 258, 271, 748, 852, 1007
HgaI GACGC 1 cut(s) 1012
HhaI GCGC 4 cut(s) 102, 711, 777, 823
Hin1I GRCGYC 2 cut(s) 827, 1004
Hin1II CATG 2 cut(s) 737, 1301
Hin6I GCGC 4 cut(s) 100, 709, 775, 821
HinP1I GCGC 4 cut(s) 100, 709, 775, 821
HincII GTYRAC 1 cut(s) 791
HindII GTYRAC 1 cut(s) 791
HindIII AAGCTT 1 cut(s) 1202
HinfI GANTC 4 cut(s) 11, 18, 107, 116
HpaII CCGG 8 cut(s) 5, 122, 149, 258, 271, 748, 852, 1007
HphI GGTGA 5 cut(s) 200, 395, 761, 1391, 1663
Hpy166II GTNNAC 2 cut(s) 523, 791
Hpy188I TCNGA 6 cut(s) 159, 639, 1069, 1233, 1601, 1662
Hpy188III TCNNGA 8 cut(s) 15, 239, 434, 446, 1507, 1584, 1688, 1759
Hpy8I GTNNAC 2 cut(s) 523, 791
Hpy99I CGWCG 2 cut(s) 829, 1006
HpyAV CCTTC 7 cut(s) 270, 315, 483, 516, 610, 661, 1409
HpyCH4III ACNGT 5 cut(s) 296, 867, 1110, 1196, 1270
HpyCH4IV ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1701
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1586, 1601, 1610, 1637
HpyF3I CTNAG 6 cut(s) 447, 817, 1017, 1068, 1505, 1598
HpySE526I ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1701
Hsp92I GRCGYC 2 cut(s) 827, 1004
Hsp92II CATG 2 cut(s) 737, 1301
HspAI GCGC 4 cut(s) 100, 709, 775, 821
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 5 cut(s) 204, 445, 742, 1009, 1636
Lsp1109I GCAGC 9 cut(s) 237, 451, 576, 796, 964, 1032, 1093, 1096, 1511
LweI GCATC 7 cut(s) 163, 427, 460, 1292, 1452, 1500, 1695
MaeI CTAG 5 cut(s) 15, 167, 267, 536, 1467
MaeII ACGT 6 cut(s) 760, 787, 796, 827, 1139, 1701
MaeIII GTNAC 3 cut(s) 571, 713, 1669
MalI GATC 7 cut(s) 70, 96, 289, 931, 1182, 1230, 1464
MbiI CCGCTC 1 cut(s) 1384
MboI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
MflI RGATCY 3 cut(s) 929, 1228, 1462
MhlI GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1438, 1591, 1606
MluCI AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1387, 1563
MlyI GAGTC 2 cut(s) 20, 116
MmeI TCCRAC 4 cut(s) 93, 606, 1299, 1658
MreI CGCCGGCG 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 6 cut(s) 516, 1208, 1223, 1364, 1454, 1493
MslI CAYNNNNRTG 1 cut(s) 1302
MspA1I CMGCKG 2 cut(s) 955, 1640
MspI CCGG 8 cut(s) 5, 122, 149, 258, 271, 748, 852, 1007
MspR9I CCNGG 3 cut(s) 748, 853, 904
Mva1269I GAATGC 1 cut(s) 1563
MvaI CCWGG 1 cut(s) 904
MvnI CGCG 5 cut(s) 47, 102, 230, 709, 1555
MwoI GCNNNNNNNGC 8 cut(s) 31, 127, 668, 818, 1586, 1601, 1610, 1637
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 2 cut(s) 748, 853
NdeII GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 737, 1301
NlaIV GGNNCC 5 cut(s) 653, 730, 731, 882, 1464
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 1669
NspI RCATGY 1 cut(s) 1301
PaeR7I CTCGAG 1 cut(s) 1584
PceI AGGCCT 2 cut(s) 53, 1751
PciSI GCTCTTC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 793, 843
PctI GAATGC 1 cut(s) 1563
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 18, 116
Pfl23II CGTACG 1 cut(s) 784
PinAI ACCGGT 1 cut(s) 148
PleI GAGTC 2 cut(s) 19, 115
PpsI GAGTC 2 cut(s) 19, 115
Ppu21I YACGTR 1 cut(s) 1702
PpuMI RGGWCCY 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 376
Psp5II RGGWCCY 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 902
PspGI CCWGG 1 cut(s) 902
PspLI CGTACG 1 cut(s) 784
PspN4I GGNNCC 5 cut(s) 653, 730, 731, 882, 1464
PspOMI GGGCCC 1 cut(s) 728
PspPI GGNCC 4 cut(s) 354, 651, 728, 729
PspPPI RGGWCCY 1 cut(s) 651
PsrI GAACNNNNNNTAC 2 cut(s) 950, 982
PstI CTGCAG 2 cut(s) 406, 1544
PstNI CAGNNNCTG 1 cut(s) 643
PsuI RGATCY 3 cut(s) 929, 1228, 1462
PvuII CAGCTG 2 cut(s) 955, 1640
RsaI GTAC 3 cut(s) 786, 1538, 1730
RsaNI GTAC 3 cut(s) 785, 1537, 1729
RseI CAYNNNNRTG 1 cut(s) 1302
SacI GAGCTC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 364
SaqAI TTAA 6 cut(s) 516, 1208, 1223, 1364, 1454, 1493
Sau3AI GATC 7 cut(s) 68, 94, 287, 929, 1180, 1228, 1462
Sau96I GGNCC 4 cut(s) 354, 651, 728, 729
SchI GAGTC 2 cut(s) 20, 116
ScrFI CCNGG 3 cut(s) 748, 853, 904
SduI GDGCHC 9 cut(s) 198, 376, 525, 694, 732, 1145, 1438, 1591, 1606
SfaNI GCATC 7 cut(s) 163, 427, 460, 1292, 1452, 1500, 1695
SfcI CTRYAG 4 cut(s) 180, 402, 1246, 1540
Sfr274I CTCGAG 1 cut(s) 1584
SgrAI CRCCGGYG 2 cut(s) 121, 148
SinI GGWCC 1 cut(s) 651
SlaI CTCGAG 1 cut(s) 1584
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 2 cut(s) 1149, 1584
SmoI CTYRAG 2 cut(s) 1149, 1584
Sse9I AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1387, 1563
SseBI AGGCCT 2 cut(s) 53, 1751
SspI AATATT 1 cut(s) 973
SspMI CTAG 5 cut(s) 15, 167, 267, 536, 1467
SstI GAGCTC 1 cut(s) 376
StuI AGGCCT 2 cut(s) 53, 1751
StyD4I CCNGG 3 cut(s) 746, 851, 902
StyI CCWWGG 2 cut(s) 721, 923
TaaI ACNGT 5 cut(s) 296, 867, 1110, 1196, 1270
TaiI ACGT 6 cut(s) 763, 790, 799, 830, 1142, 1704
TaqI TCGA 6 cut(s) 93, 290, 599, 1001, 1400, 1585
TasI AATT 7 cut(s) 413, 528, 986, 1057, 1220, 1387, 1563
TatI WGTACW 1 cut(s) 1536
TauI GCSGC 3 cut(s) 37, 230, 881
TfiI GAWTC 2 cut(s) 18, 116
Tru1I TTAA 6 cut(s) 516, 1208, 1223, 1364, 1454, 1493
Tru9I TTAA 6 cut(s) 516, 1208, 1223, 1364, 1454, 1493
TscAI CASTG 6 cut(s) 301, 784, 1336, 1438, 1611, 1660
TseFI GTSAC 1 cut(s) 1669
TseI GCWGC 9 cut(s) 250, 464, 589, 809, 952, 1045, 1081, 1084, 1524
Tsp45I GTSAC 1 cut(s) 1669
TspDTI ATGAA 1 cut(s) 1253
TspGWI ACGGA 2 cut(s) 320, 1381
TspRI CASTG 6 cut(s) 301, 784, 1336, 1438, 1611, 1660
VneI GTGCAC 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 3 cut(s) 528, 986, 1387
XbaI TCTAGA 1 cut(s) 14
XceI RCATGY 1 cut(s) 1301
XhoI CTCGAG 1 cut(s) 1584
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 5 cut(s) 15, 167, 267, 536, 1467
ZraI GACGTC 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.