MD12G1035500.v1.1

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004631
Physical Location & Seq
Reverse (-)
3869724 .. 3872280
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035500.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCTTCGCAAATTCTGGCGGCGGTAGCCGAGAGGGTCGGACCGGCGGCGAGGCGACAGGCCGTGAGCCTGACGGACGCGGCGGCGAATCGCATACGGCAGCTCCTAGAGCAGCGCCGGCGGCCTTATCTCAAGCTCGGAGTCAAGGCTCGCGGCTGCAACGGATTGTCCTACACTCTCAATTACGCAGATGATAAGGGGAAGTTCGACGAATTGGTGGAGGATAAAGGTGTGAAGATTTTGATTGATCCGAAGGCTCTGATGCACGTCATAGGAACGAAGATGGATTTTATAGATGATAAACTCAGGTCTGAGTTCGTATTCATCAATCCCAACTCTAAAGGACAGTGTGGCTGTGGGGAATCTTTCATGACAACAGGCGGCAGCGGAGCTACCAAGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

136

Amino Acids

14.72

Weight (kDa)

9.51

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 22 - 121 1.9e-18 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 80, 153
AciI CCGC 9 cut(s) 20, 23, 47, 80, 83, 121, 153, 381, 387
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 1 cut(s) 12
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 3 cut(s) 103, 136, 392
AluI AGCT 3 cut(s) 103, 136, 392
AlwI GGATC 1 cut(s) 242
AoxI GGCC 2 cut(s) 60, 122
ApeKI GCWGC 4 cut(s) 100, 112, 156, 384
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 41
AvaII GGWCC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 112, 124, 143, 396
BccI CCATC 1 cut(s) 277
BceAI ACGGC 2 cut(s) 47, 113
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 41
BmsI GCATC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bse118I RCCGGY 2 cut(s) 43, 117
BseMII CTCAG 2 cut(s) 303, 319
BseXI GCAGC 4 cut(s) 112, 124, 143, 396
Bsh1236I CGCG 2 cut(s) 80, 153
BshFI GGCC 2 cut(s) 62, 124
BsiSI CCGG 2 cut(s) 44, 118
BsnI GGCC 2 cut(s) 62, 124
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 9 cut(s) 20, 23, 47, 80, 83, 121, 153, 381, 387
BspANI GGCC 2 cut(s) 62, 124
BspCNI CTCAG 2 cut(s) 304, 318
BspFNI CGCG 2 cut(s) 80, 153
BspHI TCATGA 1 cut(s) 369
BspPI GGATC 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 43, 117
BssAI RCCGGY 2 cut(s) 43, 117
BssMI GATC 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 119, 151
BstDEI CTNAG 2 cut(s) 305, 312
BstFNI CGCG 2 cut(s) 80, 153
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 250
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
BstUI CGCG 2 cut(s) 80, 153
BstV1I GCAGC 4 cut(s) 112, 124, 143, 396
BsuRI GGCC 2 cut(s) 62, 124
BtrI CACGTC 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 119, 151
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 2 cut(s) 43, 117
Cfr13I GGNCC 1 cut(s) 41
CpoI CGGWCCG 1 cut(s) 41
CseI GACGC 1 cut(s) 86
CspI CGGWCCG 1 cut(s) 41
CviAII CATG 1 cut(s) 370
DdeI CTNAG 2 cut(s) 305, 312
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 41
FaeI CATG 1 cut(s) 373
FaiI YATR 5 cut(s) 95, 272, 293, 371, 406
FatI CATG 1 cut(s) 369
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 62, 124
HapII CCGG 2 cut(s) 44, 118
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 117
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 3 cut(s) 88, 141, 362
HpaII CCGG 2 cut(s) 44, 118
Hpy188I TCNGA 5 cut(s) 41, 140, 252, 261, 313
Hpy188III TCNNGA 1 cut(s) 370
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 2 cut(s) 247, 394
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
HpyF3I CTNAG 2 cut(s) 305, 312
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 2 cut(s) 108, 389
LpnPI CCDG 7 cut(s) 2, 44, 57, 83, 131, 292, 363
Lsp1109I GCAGC 4 cut(s) 112, 124, 143, 396
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 267
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 2 cut(s) 247, 292
MluCI AATT 3 cut(s) 12, 181, 212
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 3 cut(s) 27, 45, 214
MreI CGCCGGCG 1 cut(s) 117
MroNI GCCGGC 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 2 cut(s) 44, 118
MvnI CGCG 2 cut(s) 80, 153
MwoI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
NaeI GCCGGC 1 cut(s) 119
NdeII GATC 1 cut(s) 247
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 55
PagI TCATGA 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 88, 362
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 41
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 150
SetI ASST 7 cut(s) 105, 138, 232, 270, 311, 394, 405
SfaNI GCATC 1 cut(s) 252
SgrAI CRCCGGYG 1 cut(s) 117
SinI GGWCC 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 3 cut(s) 12, 181, 212
SsiI CCGC 9 cut(s) 20, 23, 47, 80, 83, 121, 153, 381, 387
SspMI CTAG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 207
TasI AATT 3 cut(s) 12, 181, 212
TauI GCSGC 7 cut(s) 23, 50, 83, 86, 124, 156, 384
TfiI GAWTC 2 cut(s) 88, 362
TscAI CASTG 1 cut(s) 353
TseI GCWGC 4 cut(s) 100, 112, 156, 384
TspDTI ATGAA 2 cut(s) 313, 358
TspGWI ACGGA 2 cut(s) 89, 177
TspRI CASTG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 12
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.