Rroxscaffold_6G00408250

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004631
Physical Location & Seq
Reverse (-)
30724919 .. 30726834
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408250.1

Sequence Viewer

Length: 414 bp
ATGGCTTCGCAGATTCTGGCTGCGGCGGCGGGGAGGACGGCAGCGGCGGCGAGGCGGCAAGCGGTGAGCTTGACGGATGCGGCGGCTTCTAGAATACGGCAGCTGCTAGAGCAGCGCCAGCGGTCGTATCTGAAGCTCGGGGTTAAGGCGCGTGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGATGATAAGGGAAAGTTCGATGAGTTGGTTGAAGATAAAGGCGTGAAGGTATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGAACCAAGATGGATTTTGTGGATGACAAACTCAGGTCTGAGTTTATATTTATCAATCCCAACTCGAAAGGACAATGCGGCTGTGGTGAATCTTTTATGACAACAGGTAGTACTGGAGCTACTAAGCAAAGCAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

137

Amino Acids

14.75

Weight (kDa)

9.4

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 22 - 121 7.1e-19 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 151
AclWI GGATC 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 2 cut(s) 173, 385
AgsI TTSAA 1 cut(s) 221
AluBI AGCT 4 cut(s) 69, 103, 136, 392
AluI AGCT 4 cut(s) 69, 103, 136, 392
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 137
ApeKI GCWGC 7 cut(s) 20, 41, 100, 103, 112, 156, 405
AspLEI GCGC 2 cut(s) 117, 151
AsuHPI GGTGA 2 cut(s) 76, 371
AvaI CYCGRG 1 cut(s) 137
BbvI GCAGC 6 cut(s) 7, 53, 90, 112, 124, 143
BccI CCATC 1 cut(s) 277
BceAI ACGGC 3 cut(s) 54, 113, 178
BfaI CTAG 2 cut(s) 90, 107
BfoI RGCGCY 1 cut(s) 118
BmcAI AGTACT 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 277
BmsI GCATC 2 cut(s) 67, 252
BpmI CTGGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 391
BseCI ATCGAT 1 cut(s) 246
BseGI GGATG 2 cut(s) 82, 301
BseMII CTCAG 2 cut(s) 303, 319
BseNI ACTGG 1 cut(s) 391
BseXI GCAGC 6 cut(s) 7, 53, 90, 112, 124, 143
Bsh1236I CGCG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 2 cut(s) 243, 247
BspCNI CTCAG 2 cut(s) 304, 318
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 391
BssMI GATC 2 cut(s) 243, 247
BstC8I GCNNGC 2 cut(s) 60, 119
BstDEI CTNAG 3 cut(s) 305, 312, 396
BstF5I GGATG 2 cut(s) 82, 301
BstFNI CGCG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 151
BstKTI GATC 2 cut(s) 246, 250
BstMBI GATC 2 cut(s) 243, 247
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 6 cut(s) 26, 47, 109, 112, 118, 162
BstNSI RCATGY 1 cut(s) 269
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 6 cut(s) 7, 53, 90, 112, 124, 143
Bsu15I ATCGAT 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 2 cut(s) 82, 301
Cac8I GCNNGC 2 cut(s) 60, 119
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 2 cut(s) 117, 151
ClaI ATCGAT 1 cut(s) 246
Csp6I GTAC 2 cut(s) 172, 384
CviAII CATG 1 cut(s) 266
CviQI GTAC 2 cut(s) 172, 384
DdeI CTNAG 3 cut(s) 305, 312, 396
DpnI GATC 2 cut(s) 245, 249
DpnII GATC 2 cut(s) 243, 247
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 267
FaeI CATG 1 cut(s) 269
FaiI YATR 3 cut(s) 267, 320, 371
FatI CATG 1 cut(s) 265
FauI CCCGC 1 cut(s) 22
FokI GGATG 2 cut(s) 89, 308
FspBI CTAG 2 cut(s) 90, 107
GlaI GCGC 2 cut(s) 116, 150
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 118
HhaI GCGC 2 cut(s) 117, 151
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 2 cut(s) 115, 149
HinP1I GCGC 2 cut(s) 115, 149
HinfI GANTC 2 cut(s) 13, 362
HphI GGTGA 2 cut(s) 76, 371
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 3 cut(s) 132, 261, 313
Hpy188III TCNNGA 1 cut(s) 90
Hpy8I GTNNAC 1 cut(s) 174
HpyAV CCTTC 1 cut(s) 229
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 47, 109, 112, 118, 162
HpyF3I CTNAG 3 cut(s) 305, 312, 396
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 2 cut(s) 115, 149
Kzo9I GATC 2 cut(s) 243, 247
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 5 cut(s) 2, 131, 292, 363, 372
Lsp1109I GCAGC 6 cut(s) 7, 53, 90, 112, 124, 143
LweI GCATC 2 cut(s) 67, 252
MaeI CTAG 2 cut(s) 90, 107
MalI GATC 2 cut(s) 245, 249
MboI GATC 2 cut(s) 243, 247
MboII GAAGA 1 cut(s) 233
MluCI AATT 1 cut(s) 181
MnlI CCTC 2 cut(s) 27, 45
Mph1103I ATGCAT 1 cut(s) 267
MseI TTAA 1 cut(s) 144
MspA1I CMGCKG 3 cut(s) 44, 103, 121
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 6 cut(s) 26, 47, 109, 112, 118, 162
NdeII GATC 2 cut(s) 243, 247
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PfeI GAWTC 2 cut(s) 13, 362
PspN4I GGNNCC 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 2 cut(s) 173, 385
RsaNI GTAC 2 cut(s) 172, 384
SaqAI TTAA 1 cut(s) 144
Sau3AI GATC 2 cut(s) 243, 247
ScaI AGTACT 1 cut(s) 385
SetI ASST 7 cut(s) 71, 105, 138, 240, 311, 382, 394
SfaNI GCATC 2 cut(s) 67, 252
Sse9I AATT 1 cut(s) 181
SspMI CTAG 2 cut(s) 90, 107
TaqI TCGA 3 cut(s) 207, 246, 338
TasI AATT 1 cut(s) 181
TatI WGTACW 1 cut(s) 383
TauI GCSGC 8 cut(s) 26, 29, 47, 50, 58, 83, 86, 354
TfiI GAWTC 2 cut(s) 13, 362
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 7 cut(s) 20, 41, 100, 103, 112, 156, 405
TspGWI ACGGA 1 cut(s) 89
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 1 cut(s) 269
XspI CTAG 2 cut(s) 90, 107
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.