MD14G1022300.v1.1

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004631
Physical Location & Seq
Reverse (-)
2035804 .. 2038388
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1022300.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCTTCGCAAATTCTGGCGGCGGCAGCCGAGAGGATCGGACCGGCGGCGAGGCGACAGGCCGTGAGCTTGACGGACGCGGCGTCGAATCGGATAAGGCAGCTCCTAGAGCAGCGCCGCCGGCCGTATCTTAAACTCGGCATCAAGGCTCGCGGCTGCAACGGCTTGTCCTACACTCTCAATTACGCAGATGATAAGGGGAAGTTTGACGAATTGGTGGAGGATAAAGGTGTGAAGATTTTGATCGATCCGAAGGCTCTGATGCACGTCATAGGAACGAAGATGGATTTTATAGATGATAAACTCAGGTCTGAGTTCGTATTTATCAATCCCAACTCTAAAGGACAGTGTGGTTGCGGGGAATCGTTCATGACAACAGGCAGTAGCGGGGCTACCAAGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

9.51

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 22 - 121 2.6e-17 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 80, 153
AciI CCGC 8 cut(s) 20, 23, 47, 80, 118, 153, 357, 387
AclWI GGATC 2 cut(s) 44, 242
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 83
AhdI GACNNNNNGTC 1 cut(s) 82
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 2 cut(s) 69, 103
AluI AGCT 2 cut(s) 69, 103
AlwI GGATC 2 cut(s) 44, 242
AoxI GGCC 2 cut(s) 60, 122
ApeKI GCWGC 4 cut(s) 26, 100, 112, 156
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 41
AvaII GGWCC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 38, 112, 124, 143
BccI CCATC 1 cut(s) 277
BceAI ACGGC 3 cut(s) 47, 109, 178
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 82
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 41
BmsI GCATC 2 cut(s) 150, 252
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 83
Bse118I RCCGGY 2 cut(s) 43, 120
Bse8I GATNNNNATC 1 cut(s) 242
BseCI ATCGAT 1 cut(s) 246
BseJI GATNNNNATC 1 cut(s) 242
BseMII CTCAG 2 cut(s) 303, 319
BseX3I CGGCCG 1 cut(s) 122
BseXI GCAGC 4 cut(s) 38, 112, 124, 143
Bsh1236I CGCG 2 cut(s) 80, 153
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 62, 124
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 44, 121
BsnI GGCC 2 cut(s) 62, 124
Bsp143I GATC 3 cut(s) 36, 243, 247
BspACI CCGC 8 cut(s) 20, 23, 47, 80, 118, 153, 357, 387
BspANI GGCC 2 cut(s) 62, 124
BspCNI CTCAG 2 cut(s) 304, 318
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 2 cut(s) 80, 153
BspHI TCATGA 1 cut(s) 369
BspPI GGATC 2 cut(s) 44, 242
BsrFI RCCGGY 2 cut(s) 43, 120
BssAI RCCGGY 2 cut(s) 43, 120
BssMI GATC 3 cut(s) 36, 243, 247
BssNI GRCGYC 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 348
BstACI GRCGYC 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 122, 151
BstDEI CTNAG 2 cut(s) 305, 312
BstFNI CGCG 2 cut(s) 80, 153
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 3 cut(s) 39, 246, 250
BstMBI GATC 3 cut(s) 36, 243, 247
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 4 cut(s) 26, 109, 121, 162
BstUI CGCG 2 cut(s) 80, 153
BstV1I GCAGC 4 cut(s) 38, 112, 124, 143
BstZI CGGCCG 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 2 cut(s) 62, 124
BsuTUI ATCGAT 1 cut(s) 246
BtrI CACGTC 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 122, 151
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 2 cut(s) 43, 120
Cfr13I GGNCC 1 cut(s) 41
ClaI ATCGAT 1 cut(s) 246
CpoI CGGWCCG 1 cut(s) 41
CseI GACGC 2 cut(s) 72, 86
CspI CGGWCCG 1 cut(s) 41
CviAII CATG 1 cut(s) 370
DdeI CTNAG 2 cut(s) 305, 312
DpnI GATC 3 cut(s) 38, 245, 249
DpnII GATC 3 cut(s) 36, 243, 247
DriI GACNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 122
EagI CGGCCG 1 cut(s) 122
Eam1105I GACNNNNNGTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 122
FaeI CATG 1 cut(s) 373
FaiI YATR 3 cut(s) 272, 293, 371
FatI CATG 1 cut(s) 369
FauI CCCGC 2 cut(s) 350, 380
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 62, 124
HapII CCGG 2 cut(s) 44, 121
HgaI GACGC 2 cut(s) 72, 86
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 88, 362
HpaII CCGG 2 cut(s) 44, 121
Hpy188I TCNGA 5 cut(s) 41, 93, 252, 261, 313
Hpy188III TCNNGA 1 cut(s) 370
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 2 cut(s) 247, 394
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 109, 121, 162
HpyF3I CTNAG 2 cut(s) 305, 312
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 120
KroNI GCCGGC 1 cut(s) 122
Kzo9I GATC 3 cut(s) 36, 243, 247
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 6 cut(s) 2, 44, 57, 134, 292, 363
Lsp1109I GCAGC 4 cut(s) 38, 112, 124, 143
LweI GCATC 2 cut(s) 150, 252
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 267
MalI GATC 3 cut(s) 38, 245, 249
MboI GATC 3 cut(s) 36, 243, 247
MboII GAAGA 2 cut(s) 247, 292
MluCI AATT 3 cut(s) 12, 181, 212
MnlI CCTC 3 cut(s) 27, 45, 214
MroNI GCCGGC 1 cut(s) 120
MseI TTAA 2 cut(s) 132, 406
MspI CCGG 2 cut(s) 44, 121
MvnI CGCG 2 cut(s) 80, 153
MwoI GCNNNNNNNGC 4 cut(s) 26, 109, 121, 162
NaeI GCCGGC 1 cut(s) 122
NdeII GATC 3 cut(s) 36, 243, 247
NgoMIV GCCGGC 1 cut(s) 120
NlaIII CATG 1 cut(s) 373
NmeAIII GCCGAG 2 cut(s) 55, 117
PagI TCATGA 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PdiI GCCGGC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 88, 362
PspPI GGNCC 1 cut(s) 41
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
SaqAI TTAA 2 cut(s) 132, 406
Sau3AI GATC 3 cut(s) 36, 243, 247
Sau96I GGNCC 1 cut(s) 41
SetI ASST 6 cut(s) 71, 105, 232, 270, 311, 405
SfaNI GCATC 2 cut(s) 150, 252
SinI GGWCC 1 cut(s) 41
Sse9I AATT 3 cut(s) 12, 181, 212
SsiI CCGC 8 cut(s) 20, 23, 47, 80, 118, 153, 357, 387
SspMI CTAG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 1 cut(s) 270
TaqI TCGA 2 cut(s) 86, 246
TasI AATT 3 cut(s) 12, 181, 212
TauI GCSGC 6 cut(s) 23, 26, 50, 83, 120, 156
TfiI GAWTC 2 cut(s) 88, 362
Tru1I TTAA 2 cut(s) 132, 406
Tru9I TTAA 2 cut(s) 132, 406
TscAI CASTG 1 cut(s) 353
TseI GCWGC 4 cut(s) 26, 100, 112, 156
TspDTI ATGAA 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 12
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.