Rmu_sc0004171.1_g000005

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004171.1
OG0004631
Physical Location & Seq
Reverse (-)
22351 .. 23917
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004171.1_g000005.1.cds

Sequence Viewer

Length: 417 bp
atgatggcatcccagtttctggcagcggcggcggagaggatagctccggcggcgaggcgtcaagccgtcatcttaacagacgctgcggcatctagagtacgacggctcctagagcagcgccagcggtcgtatctcaagctcggcgtcaagtcccgaggctgcaacggcttgtcgtacactctcaattacgcagatgataaaggcaagttcgacgagttgatcgaagacaagggtgtgaagatattgatcgatccaaaggctctgatgcatgttattgggaccaagatggattttgtagatgataagctcaggtccgagtttgtctttatcaatcccaactccaaaggacaatgtggttgtggtgaatctttcatgacaacaagcagtagtggagctactaaacagagtgatggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

138

Amino Acids

15.1

Weight (kDa)

9.24

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 19
AciI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
AclWI GGATC 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 58, 144
AfaI GTAC 2 cut(s) 99, 176
AfiI CCNNNNNNNGG 1 cut(s) 19
AluBI AGCT 4 cut(s) 44, 139, 307, 395
AluI AGCT 4 cut(s) 44, 139, 307, 395
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 2 cut(s) 19, 83
Ama87I CYCGRG 1 cut(s) 153
ApeKI GCWGC 4 cut(s) 23, 83, 115, 159
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 2 cut(s) 279, 312
AsuHPI GGTGA 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 279, 312
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 35, 70, 127, 146
BccI CCATC 2 cut(s) 280, 404
BceAI ACGGC 3 cut(s) 50, 119, 181
BfaI CTAG 2 cut(s) 93, 110
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
BlsI GCNGC 8 cut(s) 25, 28, 31, 52, 85, 88, 117, 161
Bme18I GGWCC 2 cut(s) 279, 312
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 2 cut(s) 279, 312
BmiI GGNNCC 2 cut(s) 107, 280
BmrI ACTGGG 1 cut(s) 7
BmsI GCATC 3 cut(s) 17, 98, 255
BmuI ACTGGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 28, 60
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 245
BsaHI GRCGYC 2 cut(s) 58, 144
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 8
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 4 cut(s) 35, 70, 127, 146
Bsh1285I CGRYCG 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 136, 292
BslI CCNNNNNNNGG 1 cut(s) 19
BsmFI GGGAC 2 cut(s) 136, 292
BsoBI CYCGRG 1 cut(s) 153
Bsp143I GATC 3 cut(s) 219, 246, 250
BspACI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
BspCNI CTCAG 1 cut(s) 321
BspDI ATCGAT 1 cut(s) 249
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 107, 280
BspPI GGATC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 219, 246, 250
BssNI GRCGYC 2 cut(s) 58, 144
BstACI GRCGYC 2 cut(s) 58, 144
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 3 cut(s) 222, 249, 253
BstMBI GATC 3 cut(s) 219, 246, 250
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
BstNSI RCATGY 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 35, 70, 127, 146
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 1 cut(s) 8
Cac8I GCNNGC 1 cut(s) 122
CaiI CAGNNNCTG 2 cut(s) 19, 83
CciI TCATGA 1 cut(s) 372
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 279, 312
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 3 cut(s) 47, 89, 133
Csp6I GTAC 2 cut(s) 98, 175
CviAII CATG 2 cut(s) 269, 373
CviJI RGCY 9 cut(s) 44, 65, 106, 139, 159, 168, 260, 307, 395
CviKI_1 RGCY 9 cut(s) 44, 65, 106, 139, 159, 168, 260, 307, 395
CviQI GTAC 2 cut(s) 98, 175
DdeI CTNAG 1 cut(s) 308
DpnI GATC 3 cut(s) 221, 248, 252
DpnII GATC 3 cut(s) 219, 246, 250
EciI GGCGGA 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 279, 312
Eco88I CYCGRG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 270
FaeI CATG 2 cut(s) 272, 376
FaiI YATR 2 cut(s) 270, 374
FaqI GGGAC 2 cut(s) 136, 292
FatI CATG 2 cut(s) 268, 372
Fnu4HI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
Fsp4HI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
FspBI CTAG 2 cut(s) 93, 110
GlaI GCGC 1 cut(s) 119
GluI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
HaeII RGCGCY 1 cut(s) 121
HapII CCGG 1 cut(s) 47
HgaI GACGC 3 cut(s) 47, 89, 133
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 2 cut(s) 58, 144
Hin1II CATG 2 cut(s) 272, 376
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 2 cut(s) 264, 316
Hpy188III TCNNGA 3 cut(s) 93, 153, 373
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 2 cut(s) 105, 215
HpyCH4V TGCA 2 cut(s) 162, 268
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
HpyF3I CTNAG 1 cut(s) 308
Hsp92I GRCGYC 2 cut(s) 58, 144
Hsp92II CATG 2 cut(s) 272, 376
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 219, 246, 250
LmnI GCTCC 3 cut(s) 49, 111, 392
LpnPI CCDG 5 cut(s) 5, 26, 60, 134, 295
Lsp1109I GCAGC 4 cut(s) 35, 70, 127, 146
LweI GCATC 3 cut(s) 17, 98, 255
MaeI CTAG 2 cut(s) 93, 110
MalI GATC 3 cut(s) 221, 248, 252
MboI GATC 3 cut(s) 219, 246, 250
MboII GAAGA 2 cut(s) 236, 250
MluCI AATT 1 cut(s) 184
MnlI CCTC 3 cut(s) 30, 48, 149
Mph1103I ATGCAT 1 cut(s) 270
MseI TTAA 2 cut(s) 74, 415
MspA1I CMGCKG 2 cut(s) 26, 124
MspI CCGG 1 cut(s) 47
MwoI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
NdeII GATC 3 cut(s) 219, 246, 250
NlaIII CATG 2 cut(s) 272, 376
NlaIV GGNNCC 2 cut(s) 107, 280
NmeAIII GCCGAG 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 272
PagI TCATGA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 19
PkrI GCNGC 8 cut(s) 25, 28, 31, 52, 85, 88, 117, 161
PspN4I GGNNCC 2 cut(s) 107, 280
PspPI GGNCC 2 cut(s) 279, 312
PstNI CAGNNNCTG 2 cut(s) 19, 83
RsaI GTAC 2 cut(s) 99, 176
RsaNI GTAC 2 cut(s) 98, 175
SaqAI TTAA 2 cut(s) 74, 415
SatI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
Sau3AI GATC 3 cut(s) 219, 246, 250
Sau96I GGNCC 2 cut(s) 279, 312
SetI ASST 5 cut(s) 46, 141, 309, 314, 397
SfaNI GCATC 3 cut(s) 17, 98, 255
SinI GGWCC 2 cut(s) 279, 312
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 1 cut(s) 184
SsiI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
SspMI CTAG 2 cut(s) 93, 110
TaqI TCGA 3 cut(s) 210, 222, 249
TasI AATT 1 cut(s) 184
TauI GCSGC 4 cut(s) 29, 32, 53, 89
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 74, 415
Tru9I TTAA 2 cut(s) 74, 415
TseI GCWGC 4 cut(s) 23, 83, 115, 159
TspDTI ATGAA 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 19
VpaK11BI GGWCC 2 cut(s) 279, 312
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 272
XspI CTAG 2 cut(s) 93, 110
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.