Rw6G014830

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004631
Physical Location & Seq
Forward (+)
27696771 .. 27698980
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014830.1

Sequence Viewer

Length: 420 bp
ATGATGATGGCATCCCAGTTTCTGGCAGCGGCGGCGGAGAGGATAGCTCCGGCGGCGAGGCGTCAAGCCGTCATCTTAACAGACGCTGCGGCATCTAGAGTACGACGGCTCCTAGAGCAGCGCCAGCGGTCGTATCTCAAGCTCGGCGTCAAGTCCCGAGGCTGCAACGGCTTGTCGTACACTCTCAATTACGCAGATGATAAAGGCAAGTTCGACGAGTTGATCGAAGACAAGGGTGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTTATTGGGACCAAGATGGATTTTGTAGATGATAAGCTCAGGTCCGAGTTTGTCTTTATCAATCCCAACTCCAAAGGACAATGTGGTTGTGGTGAATCTTTCATGACAACAAGCAGTAGTGGAGCTACTAAACAGAGTGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

139

Amino Acids

15.18

Weight (kDa)

9.4

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 25 - 123 1.2e-18 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 22
AciI CCGC 6 cut(s) 29, 32, 35, 53, 89, 127
AclWI GGATC 1 cut(s) 248
AcyI GRCGYC 2 cut(s) 61, 147
AfaI GTAC 2 cut(s) 102, 179
AfiI CCNNNNNNNGG 1 cut(s) 22
AluBI AGCT 4 cut(s) 47, 142, 310, 398
AluI AGCT 4 cut(s) 47, 142, 310, 398
AlwI GGATC 1 cut(s) 248
AlwNI CAGNNNCTG 2 cut(s) 22, 86
Ama87I CYCGRG 1 cut(s) 156
ApeKI GCWGC 4 cut(s) 26, 86, 118, 162
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 282, 315
AsuHPI GGTGA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 2 cut(s) 282, 315
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 38, 73, 130, 149
BccI CCATC 1 cut(s) 283
BceAI ACGGC 3 cut(s) 53, 122, 184
BfaI CTAG 2 cut(s) 96, 113
BfoI RGCGCY 1 cut(s) 124
BisI GCNGC 8 cut(s) 27, 30, 33, 54, 87, 90, 119, 163
BlsI GCNGC 8 cut(s) 28, 31, 34, 55, 88, 91, 120, 164
Bme18I GGWCC 2 cut(s) 282, 315
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 282, 315
BmiI GGNNCC 2 cut(s) 110, 283
BmrI ACTGGG 1 cut(s) 10
BmsI GCATC 3 cut(s) 20, 101, 258
BmuI ACTGGG 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 31, 63
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 248
BsaHI GRCGYC 2 cut(s) 61, 147
BsaJI CCNNGG 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 11
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 16
BseXI GCAGC 4 cut(s) 38, 73, 130, 149
Bsh1285I CGRYCG 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 2 cut(s) 139, 295
BslI CCNNNNNNNGG 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 139, 295
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 3 cut(s) 222, 249, 253
BspACI CCGC 6 cut(s) 29, 32, 35, 53, 89, 127
BspCNI CTCAG 1 cut(s) 324
BspDI ATCGAT 1 cut(s) 252
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 110, 283
BspPI GGATC 1 cut(s) 248
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 3 cut(s) 222, 249, 253
BssNI GRCGYC 2 cut(s) 61, 147
BstACI GRCGYC 2 cut(s) 61, 147
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 311
BstF5I GGATG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 3 cut(s) 225, 252, 256
BstMBI GATC 3 cut(s) 222, 249, 253
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 5 cut(s) 32, 53, 115, 124, 168
BstNSI RCATGY 1 cut(s) 275
BstV1I GCAGC 4 cut(s) 38, 73, 130, 149
BstV2I GAAGAC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 1 cut(s) 11
Cac8I GCNNGC 1 cut(s) 125
CaiI CAGNNNCTG 2 cut(s) 22, 86
CciI TCATGA 1 cut(s) 375
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 282, 315
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 3 cut(s) 50, 92, 136
Csp6I GTAC 2 cut(s) 101, 178
CviAII CATG 2 cut(s) 272, 376
CviJI RGCY 9 cut(s) 47, 68, 109, 142, 162, 171, 263, 310, 398
CviKI_1 RGCY 9 cut(s) 47, 68, 109, 142, 162, 171, 263, 310, 398
CviQI GTAC 2 cut(s) 101, 178
DdeI CTNAG 1 cut(s) 311
DpnI GATC 3 cut(s) 224, 251, 255
DpnII GATC 3 cut(s) 222, 249, 253
EciI GGCGGA 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 282, 315
Eco88I CYCGRG 1 cut(s) 156
EcoT22I ATGCAT 1 cut(s) 273
FaeI CATG 2 cut(s) 275, 379
FaiI YATR 2 cut(s) 273, 377
FaqI GGGAC 2 cut(s) 139, 295
FatI CATG 2 cut(s) 271, 375
Fnu4HI GCNGC 8 cut(s) 27, 30, 33, 54, 87, 90, 119, 163
Fsp4HI GCNGC 8 cut(s) 27, 30, 33, 54, 87, 90, 119, 163
FspBI CTAG 2 cut(s) 96, 113
GlaI GCGC 1 cut(s) 122
GluI GCNGC 8 cut(s) 27, 30, 33, 54, 87, 90, 119, 163
HaeII RGCGCY 1 cut(s) 124
HapII CCGG 1 cut(s) 50
HgaI GACGC 3 cut(s) 50, 92, 136
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 2 cut(s) 61, 147
Hin1II CATG 2 cut(s) 275, 379
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HinfI GANTC 1 cut(s) 368
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 2 cut(s) 267, 319
Hpy188III TCNNGA 3 cut(s) 96, 156, 376
Hpy8I GTNNAC 1 cut(s) 180
Hpy99I CGWCG 2 cut(s) 108, 218
HpyCH4V TGCA 2 cut(s) 165, 271
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 53, 115, 124, 168
HpyF3I CTNAG 1 cut(s) 311
Hsp92I GRCGYC 2 cut(s) 61, 147
Hsp92II CATG 2 cut(s) 275, 379
HspAI GCGC 1 cut(s) 121
Kzo9I GATC 3 cut(s) 222, 249, 253
LmnI GCTCC 3 cut(s) 52, 114, 395
LpnPI CCDG 6 cut(s) 8, 29, 63, 137, 298, 399
Lsp1109I GCAGC 4 cut(s) 38, 73, 130, 149
LweI GCATC 3 cut(s) 20, 101, 258
MaeI CTAG 2 cut(s) 96, 113
MalI GATC 3 cut(s) 224, 251, 255
MboI GATC 3 cut(s) 222, 249, 253
MboII GAAGA 2 cut(s) 239, 253
MluCI AATT 1 cut(s) 187
MnlI CCTC 3 cut(s) 33, 51, 152
Mph1103I ATGCAT 1 cut(s) 273
MseI TTAA 2 cut(s) 77, 418
MspA1I CMGCKG 2 cut(s) 29, 127
MspI CCGG 1 cut(s) 50
MwoI GCNNNNNNNGC 5 cut(s) 32, 53, 115, 124, 168
NdeII GATC 3 cut(s) 222, 249, 253
NlaIII CATG 2 cut(s) 275, 379
NlaIV GGNNCC 2 cut(s) 110, 283
NmeAIII GCCGAG 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 273
NspI RCATGY 1 cut(s) 275
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 368
PflMI CCANNNNNTGG 1 cut(s) 22
PkrI GCNGC 8 cut(s) 28, 31, 34, 55, 88, 91, 120, 164
PspN4I GGNNCC 2 cut(s) 110, 283
PspPI GGNCC 2 cut(s) 282, 315
PstNI CAGNNNCTG 2 cut(s) 22, 86
RsaI GTAC 2 cut(s) 102, 179
RsaNI GTAC 2 cut(s) 101, 178
SaqAI TTAA 2 cut(s) 77, 418
SatI GCNGC 8 cut(s) 27, 30, 33, 54, 87, 90, 119, 163
Sau3AI GATC 3 cut(s) 222, 249, 253
Sau96I GGNCC 2 cut(s) 282, 315
SetI ASST 5 cut(s) 49, 144, 312, 317, 400
SfaNI GCATC 3 cut(s) 20, 101, 258
SinI GGWCC 2 cut(s) 282, 315
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 187
SsiI CCGC 6 cut(s) 29, 32, 35, 53, 89, 127
SspMI CTAG 2 cut(s) 96, 113
TaqI TCGA 3 cut(s) 213, 225, 252
TasI AATT 1 cut(s) 187
TauI GCSGC 4 cut(s) 32, 35, 56, 92
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 2 cut(s) 77, 418
Tru9I TTAA 2 cut(s) 77, 418
TseI GCWGC 4 cut(s) 26, 86, 118, 162
TspDTI ATGAA 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 282, 315
XbaI TCTAGA 1 cut(s) 95
XceI RCATGY 1 cut(s) 275
XspI CTAG 2 cut(s) 96, 113
Zsp2I ATGCAT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.