FvH4_6g24600

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003093
Physical Location & Seq
Reverse (-)
18666897 .. 18675695
8799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24600.t2

Sequence Viewer

Length: 3843 bp
ATGTCGCCGGCGGCGGCGGACTTCCGGTCCCCGATTACCTCCCCACTATCCAAACCCTCCGCCCATCCTCAAAACCCCTCGGACGAGACGCCTTCCTCTAACCCCTTCAATTTCAATGCTCTCGGCTCCGACGGTCCAAATGTGCCGTCACGATCAGCTCGGTCGCGGCCGAGGCTGGTGAAGCATAGGAAGCAGCACGCGAGGTCGAGAACTTGGCTGGCCGAGCCGGGCCCGGAGCTCAACCCGTTTCGCTCGGTAAGTGCTGCTGATACTAGTGCTTCTAATCAGTTCACTTTTTCGAGTGTTGGTGAGGGCTTTGTGTTTGGCGCTAAATTGAGCGGTATTGGTGAGAAGGCTGGGGATGGTGGAGATTTCAATGAACTTGAGAGTGTCGCTAATCAGGATGATTTGGGACCGAGTTCTAGGGAGAGTGGAGCAGCTAAGACTGATGCAAATTTGAGTAGCGAAAATGGAGAATCTGCTGGATATGTTGAGAATCTTGGTGACTCAGGGTGTAGTTCAAATGTAGGGGAACGTGAATATTGTGAAAATGAGGGGACTGAGAGTCAGGGAACTGGTAATGAAGCTGAGGGAAGGAGCTATAGTGGTGTTGGTTTTGTGTTTGGTTCCAGTTGGGGAGGTTCAAGTTCATGTTTTCATTCGCTAAAGAACGACTCTAGTGGAAATGTGGAAAATTTGGTCGATGAAGTTGGCAGCAAGATGAAAGCTGATTCTTTCAAATTTAATAATGGGGAGCGCTCGTGCTCAAACAAGGTTCATGAGGAGGGAAGTTTTGCATTTGGAAGTGGGGGCTTGACGGATTCCAAGCTACCTAATTGTCAAGGTGAGGTGAAACCAAGTGATGAAGATTTAGGAGATTGCAAGTCCAAGTCAAACAGTCTGAATGATAAACTGAGTTCTGATTTCAGTGGCAAGTGTAAGCTTGTATTTGAAAGTTGTGAGGTAACTGATGCATCTAGTCATAAAAGTACTAACCATGTTGTTTTTGGAAGCGAGAATAACACGGCTGGTACCACTGGAGCGACTTCTAGTTCTGCTCAGTTTACATTTCAGACGGGTCTTGGCGTCCAAACAAATGGTTGTGCTTCGAAAGCATCATCTGCGGGTGGAGTGAAAGGTAAAGATGGAAGTATCTTTACTAGCTCTCTAGACGGATTTGGTGTGTGCTTTGAGGACTTCAACACTCCATTGCGGTTTTCTTCTTGTCTCAAAGAAACTCTAATTCCTGAAATAAATAAAGCGAACATCAATGTGAAGGGTAGATCAGTTAGGGACAAAAGATCAAGGAAGACAAGGGCAAAGTTGAAGAAACCTTCTCTGAGTAAGCAACGGCCAGCTCAGGATAATGTTCCCAAGGAAAGTAGTTGCCAAGGAACCCCAGACTCTCCTGGTTGTTGCTCCCCCATGGATTTCTCTCCTTATGGAGAAACTATAGTTGTTGATCCACATTTAAGGGAATCCTCTGCAACGTCTAATGATTCCATCCACCTAGGCAGTGATACTGCACCTTCTGTCTCCGATGCAACAGTTACTATTGGACCTAATGGTGATGAGGGAGAATTGGCTGCTAAAGGTGATCACATATATGGAAAGGCCGTTGACGAGAATTCTGGTCATTATGGTGAAAGAATATTTGTTAACCATTGTTCCACTAAAGGGTCTGGTTCTGGAGCTGAAACTCCCTGCTTTAGCTCCAAAAATGAGCATGTCAACGGTATCAGTCGTGCTGGTAAGGATTCACAAGACGTTAGAGCTGATTTTGGTCTGAATGTAGAGAATCAAGAACAGGATTGTGGAACACCGTGTTTTGCTTCTGGTTTTGAAAATATGGAGAAGAAACCATTCACCTTTGTTGCGTCATCTTCTGCAGAACGAAGTCCAATAGTGGCAAGGCGTCTACTTCGCAGAGTGAAAAATAAGAAGAAAGTCGGTTGTAATACATTTGTTATAACTCCAAGTACAAATGTTGAGTTTGGAGCTGACAAATCTGCACCCCACTCTAGCACATCATTGTCTTCTGATGTGGTAGAAAAACCTGAGCCGCATGAACAAGTTACGAAAGTGCATATCCCTTCTACTGATGCAACTCATGAGACTTGTGAGAAGTGGCGAATCAGGGGCAACAAAGCTTATAGGAACAAAGACTTTTCGAAAGCTGAAGAATTTTACACACAGGGAATAGTTTCTGTTCCTTCAAGTGAAAGGTCGGGAAGCTGCCTTAAGCCCCTTTTACTATGCTATAGCAATCGAGCGGCAACACGGATGTATATGGGCAAGATAAGGGAAGCTTTAGGGGACTGTATGATGGCTACTGCGCTAGACCCCAACTTTCTCAAAGTTCAAATGAGAGCTGCAAATTTTCATCTTCTTCTGGGTGAAGTTGAAAATGCACAACGGTACTTCAATAATTGCTTGGAATTCGGAACTGGTGTGTGTTTGGATCGAAGAATTGTAATAGATTCTGCTGATGGCATACAGAAGGCTCAGAAAGTGACTGAGTATATGAATCGCTCTGCCAAGCTTCTTGAACAGAAAACTACAGATGCGGCTCTTAGTGCCTTGGAAATAGTTTCTGAGGCCTTGTGTATTAGTGACTACTCAGAAAAGTTACTTGAAATGAAGGCAGAGGCTCTGTGTATGTTGAGGAGATATGAAGAGGCAATTCAACTGTGTGAACATAGTATGTGTTTTGCTGAGAAGAACTTTTCTTCGCTGAACAGTGTAGTAAATATGGATGGTGCCGGATGTGAAGATCATCCATATGCTAGGTTGTGGAGGTGGTTCTTCATATCCAAGTCTTACTTCCATTTGGGAAGGTTTGAGGCAGCTCTTAATTTACTTGAGAAGCTAGAGCAAGTGGGATCTATCAAAGACAGGTATGAGAGTAAGAACTTGGAATCATCAATTTCATTAGCTGCCACCATACGTGAACTGTTAAGCCACAAGGACGCAGGGAATGACGCATTTAGATCCGGAAAACATACAGAAGCAGTGGAACATTATACTGTTGCTTTGTCTCGCAATATTGGATCTCGGCCATTTTCTGCAATCTGTTTGTGCAACCGTGCTGCAGCACATCAAGCTTTGGGACAAATTACTGATGCCATTGCTGACTGCAGTCTTGCAATAGCTCTTGATGGAAATTACGCTAAGGCAGTTTCTAGAAGAGCTACCTTACATGAGATGATACGAGACTATGGACAGGCTGCTAGTGATCTCCAAAGACTTATATTTGTACTGGAAAACCAATCTGGTGACAAGGCCAAAGAACCTAACTCACAAGGTAGCTCCACAGGTCGCACACAAGAATTAAGGAATGCTTATCGACATATGCCTTTAATGGAAGAAGAAGCTAAGAAAGGAATCCCCTTCAATTTTTACATCATTCTGGGAATTAAACCATCTGACTCAAGTGCTGACATTAAGAAGGCTTATCGTAAGGCTGCTCTGAAGCATCATCCTGACAAGGCTGGTCAGTTCTTGGCAAGAAGTGAGAGTGGAGATGAAGGGCGACTCTGGAAAGAAATCTCTCTTGAGGTTCACAAGGATGCTGACAGACTCTTTAAAATGATTGGAGAGGCATATGCAGTACTTTCAGATCCCAACAAGCGCTCAGAATATGATTTTGACGAAGACATAAGAAAATCAGCAAAGGAAAGCAAGGATCACGGCATCTACAGAAAACCTTCAGATGTTCAGAGTCCTAGACGCAGCAGTAATAGAAAACCAGATTTCTATAGCTCTCCATTTGAGAGAAGCTCTTATTGTCGGAACAGTAGGGAGAGTTGGAGGACATATGGAGATTCATTTTCACGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

1281

Amino Acids

139.53

Weight (kDa)

6.43

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1132 - 1164 1.1e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1970
Acc65I GGTACC 1 cut(s) 1031
AccB1I GGYRCC 2 cut(s) 1031, 2759
AccBSI CCGCTC 2 cut(s) 339, 2273
AccI GTMKAC 1 cut(s) 1918
AccII CGCG 2 cut(s) 166, 200
AccIII TCCGGA 1 cut(s) 2993
AclWI GGATC 7 cut(s) 1457, 2469, 2890, 2985, 3058, 3614, 3693
AcoI YGGCCR 4 cut(s) 167, 219, 1352, 3056
AcsI RAATTY 7 cut(s) 454, 694, 740, 1627, 2183, 2377, 2438
AcuI CTGAAG 3 cut(s) 2199, 3491, 3693
AcyI GRCGYC 3 cut(s) 89, 1086, 1915
AfaI GTAC 6 cut(s) 991, 1033, 1981, 2420, 3258, 3612
AfeI AGCGCT 2 cut(s) 758, 3632
AfiI CCNNNNNNNGG 1 cut(s) 1677
AflII CTTAAG 1 cut(s) 2240
AhdI GACNNNNNGTC 2 cut(s) 25, 564
AhlI ACTAGT 1 cut(s) 272
AjnI CCWGG 1 cut(s) 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 2807, 2839
Alw21I GWGCWC 2 cut(s) 240, 767
Alw26I GTCTC 6 cut(s) 80, 1232, 1540, 2108, 3042, 3207
AlwI GGATC 7 cut(s) 1457, 2469, 2890, 2985, 3058, 3614, 3693
Aor13HI TCCGGA 1 cut(s) 2993
Aor51HI AGCGCT 2 cut(s) 758, 3632
AoxI GGCC 8 cut(s) 167, 219, 229, 1352, 1614, 2598, 3056, 3282
ApaI GGGCCC 1 cut(s) 233
ApoI RAATTY 7 cut(s) 454, 694, 740, 1627, 2183, 2377, 2438
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 3 cut(s) 1862, 2203, 3706
Asp718I GGTACC 1 cut(s) 1031
AspA2I CCTAGG 1 cut(s) 1510
AspLEI GCGC 4 cut(s) 329, 759, 2338, 3633
AspS9I GGNCC 6 cut(s) 27, 134, 229, 230, 413, 1559
AsuC2I CCSGG 2 cut(s) 228, 233
AsuII TTCGAA 2 cut(s) 1109, 2171
AvaII GGWCC 4 cut(s) 27, 134, 413, 1559
AvrII CCTAGG 1 cut(s) 1510
BaeGI GKGCMC 1 cut(s) 233
BanI GGYRCC 2 cut(s) 1031, 2759
BanII GRGCYC 2 cut(s) 233, 240
BarI GAAGNNNNNNTAC 2 cut(s) 1141, 1173
BauI CACGAG 1 cut(s) 760
BbsI GAAGAC 3 cut(s) 1316, 2028, 3660
Bbv12I GWGCWC 2 cut(s) 240, 767
BbvCI CCTCAGC 1 cut(s) 588
BccI CCATC 9 cut(s) 72, 356, 1139, 1511, 2320, 2483, 2750, 3152, 3430
BceAI ACGGC 5 cut(s) 130, 1041, 1367, 1601, 3706
BciT130I CCWGG 1 cut(s) 1410
BclI TGATCA 1 cut(s) 1597
BcnI CCSGG 2 cut(s) 228, 233
BcoDI GTCTC 6 cut(s) 80, 1232, 1540, 2108, 3042, 3207
BcuI ACTAGT 1 cut(s) 272
BfmI CTRYAG 9 cut(s) 601, 1452, 1887, 2260, 2559, 3090, 3136, 3697, 3757
BfoI RGCGCY 3 cut(s) 330, 760, 3634
BfrI CTTAAG 1 cut(s) 2240
BlnI CCTAGG 1 cut(s) 1510
BmcAI AGTACT 2 cut(s) 991, 3612
Bme1390I CCNGG 3 cut(s) 228, 233, 1410
Bme18I GGWCC 4 cut(s) 27, 134, 413, 1559
BmeRI GACNNNNNGTC 2 cut(s) 25, 564
BmgT120I GGNCC 6 cut(s) 27, 134, 229, 230, 413, 1559
BmiI GGNNCC 8 cut(s) 29, 127, 231, 414, 628, 1033, 1396, 2761
BmrFI CCNGG 3 cut(s) 228, 233, 1410
BoxI GACNNNNGTC 1 cut(s) 3138
BpiI GAAGAC 3 cut(s) 1316, 2028, 3660
BplI GAGNNNNNCTC 2 cut(s) 3764, 3796
BpmI CTGGAG 2 cut(s) 1059, 1710
Bpu10I CCTNAGC 4 cut(s) 588, 1359, 2058, 3171
Bpu14I TTCGAA 2 cut(s) 1109, 2171
BpuEI CTTGAG 4 cut(s) 404, 2882, 3415, 3575
BpuMI CCSGG 2 cut(s) 228, 233
BsaAI YACGTR 1 cut(s) 2948
BsaHI GRCGYC 3 cut(s) 89, 1086, 1915
BsaJI CCNNGG 7 cut(s) 78, 170, 1374, 1390, 1425, 1510, 2580
BsaWI WCCGGW 2 cut(s) 24, 2993
BsaXI ACNNNNNCTCC 4 cut(s) 589, 619, 746, 776
Bsc4I CCNNNNNNNGG 1 cut(s) 1677
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 5 cut(s) 580, 630, 1042, 2452, 3264
Bse3DI GCAATG 2 cut(s) 1208, 3126
BseAI TCCGGA 1 cut(s) 2993
BseBI CCWGG 1 cut(s) 1410
BseDI CCNNGG 7 cut(s) 78, 170, 1374, 1390, 1425, 1510, 2580
BseLI CCNNNNNNNGG 1 cut(s) 1677
BseMI GCAATG 2 cut(s) 1208, 3126
BseNI ACTGG 5 cut(s) 580, 630, 1042, 2452, 3264
BseRI GAGGAG 2 cut(s) 797, 2680
BseSI GKGCMC 1 cut(s) 233
BseX3I CGGCCG 1 cut(s) 167
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 2 cut(s) 1509, 1995
Bsh1236I CGCG 2 cut(s) 166, 200
Bsh1285I CGRYCG 2 cut(s) 164, 170
BshFI GGCC 8 cut(s) 169, 221, 231, 1354, 1616, 2600, 3058, 3284
BshNI GGYRCC 2 cut(s) 1031, 2759
BsiEI CGRYCG 2 cut(s) 164, 170
BsiHKAI GWGCWC 2 cut(s) 240, 767
BsiSI CCGG 6 cut(s) 8, 25, 227, 233, 2763, 2994
BslFI GGGAC 6 cut(s) 13, 426, 571, 1307, 2330, 3123
BslI CCNNNNNNNGG 1 cut(s) 1677
BsmAI GTCTC 6 cut(s) 80, 1232, 1540, 2108, 3042, 3207
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 6 cut(s) 13, 426, 571, 1307, 2330, 3123
BsmI GAATGC 1 cut(s) 3343
BsnI GGCC 8 cut(s) 169, 221, 231, 1354, 1616, 2600, 3058, 3284
Bsp119I TTCGAA 2 cut(s) 1109, 2171
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 233, 240, 767
Bsp13I TCCGGA 1 cut(s) 2993
Bsp19I CCATGG 1 cut(s) 1425
BspANI GGCC 8 cut(s) 169, 221, 231, 1354, 1616, 2600, 3058, 3284
BspEI TCCGGA 1 cut(s) 2993
BspFNI CGCG 2 cut(s) 166, 200
BspHI TCATGA 2 cut(s) 778, 2110
BspLI GGNNCC 8 cut(s) 29, 127, 231, 414, 628, 1033, 1396, 2761
BspMAI CTGCAG 3 cut(s) 1891, 3094, 3140
BspPI GGATC 7 cut(s) 1457, 2469, 2890, 2985, 3058, 3614, 3693
BspQI GCTCTTC 1 cut(s) 3181
BspT104I TTCGAA 2 cut(s) 1109, 2171
BspT107I GGYRCC 2 cut(s) 1031, 2759
BspTI CTTAAG 1 cut(s) 2240
BsrBI CCGCTC 2 cut(s) 339, 2273
BsrDI GCAATG 2 cut(s) 1208, 3126
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 5 cut(s) 580, 630, 1042, 2452, 3264
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 7 cut(s) 78, 170, 1374, 1390, 1425, 1510, 2580
BssNI GRCGYC 3 cut(s) 89, 1086, 1915
BssSI CACGAG 1 cut(s) 760
BssT1I CCWWGG 5 cut(s) 1374, 1390, 1425, 1510, 2580
Bst2BI CACGAG 1 cut(s) 760
Bst2UI CCWGG 1 cut(s) 1410
Bst6I CTCTTC 2 cut(s) 2670, 3181
BstACI GRCGYC 3 cut(s) 89, 1086, 1915
BstAFI CTTAAG 1 cut(s) 2240
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstBAI YACGTR 1 cut(s) 2948
BstBI TTCGAA 2 cut(s) 1109, 2171
BstC8I GCNNGC 4 cut(s) 9, 198, 219, 1356
BstDSI CCRYGG 1 cut(s) 1425
BstFNI CGCG 2 cut(s) 166, 200
BstH2I RGCGCY 3 cut(s) 330, 760, 3634
BstHHI GCGC 4 cut(s) 329, 759, 2338, 3633
BstMAI GTCTC 6 cut(s) 80, 1232, 1540, 2108, 3042, 3207
BstMCI CGRYCG 2 cut(s) 164, 170
BstMWI GCNNNNNNNGC 8 cut(s) 172, 181, 190, 223, 1112, 1121, 2576, 3101
BstNI CCWGG 1 cut(s) 1410
BstNSI RCATGY 1 cut(s) 1730
BstPAI GACNNNNGTC 1 cut(s) 3138
BstSCI CCNGG 3 cut(s) 226, 231, 1408
BstSFI CTRYAG 9 cut(s) 601, 1452, 1887, 2260, 2559, 3090, 3136, 3697, 3757
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 2 cut(s) 166, 200
BstV2I GAAGAC 3 cut(s) 1316, 2028, 3660
BstX2I RGATCY 4 cut(s) 2882, 2990, 3050, 3619
BstXI CCANNNNNNTGG 1 cut(s) 1097
BstYI RGATCY 4 cut(s) 2882, 2990, 3050, 3619
BstZI CGGCCG 1 cut(s) 167
BsuRI GGCC 8 cut(s) 169, 221, 231, 1354, 1616, 2600, 3058, 3284
BtgI CCRYGG 1 cut(s) 1425
BtsI GCAGTG 2 cut(s) 1522, 3018
BtsIMutI CAGTG 5 cut(s) 934, 1035, 1522, 2746, 3018
Cac8I GCNNGC 4 cut(s) 9, 198, 219, 1356
CciI TCATGA 2 cut(s) 778, 2110
CfoI GCGC 4 cut(s) 329, 759, 2338, 3633
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 6 cut(s) 27, 134, 229, 230, 413, 1559
CseI GACGC 7 cut(s) 97, 1075, 1866, 1904, 2978, 2990, 3738
Csp6I GTAC 6 cut(s) 990, 1032, 1980, 2419, 3257, 3611
CviAII CATG 8 cut(s) 651, 779, 998, 1426, 1727, 2066, 2111, 3200
CviQI GTAC 6 cut(s) 990, 1032, 1980, 2419, 3257, 3611
DraI TTTAAA 1 cut(s) 3586
DriI GACNNNNNGTC 2 cut(s) 25, 564
EaeI YGGCCR 4 cut(s) 167, 219, 1352, 3056
EagI CGGCCG 1 cut(s) 167
Eam1104I CTCTTC 2 cut(s) 2670, 3181
Eam1105I GACNNNNNGTC 2 cut(s) 25, 564
EarI CTCTTC 2 cut(s) 2670, 3181
EciI GGCGGA 2 cut(s) 32, 49
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 167
Eco130I CCWWGG 5 cut(s) 1374, 1390, 1425, 1510, 2580
Eco147I AGGCCT 1 cut(s) 2600
Eco24I GRGCYC 2 cut(s) 233, 240
Eco47I GGWCC 4 cut(s) 27, 134, 413, 1559
Eco47III AGCGCT 2 cut(s) 758, 3632
Eco52I CGGCCG 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 3 cut(s) 2199, 3491, 3693
EcoICRI GAGCTC 1 cut(s) 238
EcoRI GAATTC 2 cut(s) 1627, 2438
EcoRII CCWGG 1 cut(s) 1408
EcoT14I CCWWGG 5 cut(s) 1374, 1390, 1425, 1510, 2580
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 2 cut(s) 233, 240
ErhI CCWWGG 5 cut(s) 1374, 1390, 1425, 1510, 2580
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 8 cut(s) 654, 782, 1001, 1429, 1730, 2069, 2114, 3203
FalI AAGNNNNNCTT 6 cut(s) 2293, 2325, 2807, 2839, 3325, 3357
FaqI GGGAC 6 cut(s) 13, 426, 571, 1307, 2330, 3123
FatI CATG 8 cut(s) 650, 778, 997, 1425, 1726, 2065, 2110, 3199
FauI CCCGC 1 cut(s) 1117
FauNDI CATATG 4 cut(s) 2782, 3351, 3604, 3817
FbaI TGATCA 1 cut(s) 1597
FblI GTMKAC 1 cut(s) 1918
FriOI GRGCYC 2 cut(s) 233, 240
GlaI GCGC 4 cut(s) 328, 758, 2337, 3632
GsaI CCCAGC 1 cut(s) 360
GsuI CTGGAG 2 cut(s) 1059, 1710
HaeII RGCGCY 3 cut(s) 330, 760, 3634
HaeIII GGCC 8 cut(s) 169, 221, 231, 1354, 1616, 2600, 3058, 3284
HapII CCGG 6 cut(s) 8, 25, 227, 233, 2763, 2994
HgaI GACGC 7 cut(s) 97, 1075, 1866, 1904, 2978, 2990, 3738
HhaI GCGC 4 cut(s) 329, 759, 2338, 3633
Hin1I GRCGYC 3 cut(s) 89, 1086, 1915
Hin1II CATG 8 cut(s) 654, 782, 1001, 1429, 1730, 2069, 2114, 3203
Hin6I GCGC 4 cut(s) 327, 757, 2336, 3631
HinP1I GCGC 4 cut(s) 327, 757, 2336, 3631
HincII GTYRAC 3 cut(s) 1621, 1660, 1732
HindII GTYRAC 3 cut(s) 1621, 1660, 1732
HindIII AAGCTT 5 cut(s) 941, 2148, 2307, 2540, 3102
HpaI GTTAAC 1 cut(s) 1660
HpaII CCGG 6 cut(s) 8, 25, 227, 233, 2763, 2994
Hpy166II GTNNAC 9 cut(s) 291, 1065, 1621, 1660, 1732, 1919, 2696, 2951, 3562
Hpy8I GTNNAC 9 cut(s) 291, 1065, 1621, 1660, 1732, 1919, 2696, 2951, 3562
Hpy99I CGWCG 1 cut(s) 134
HpyCH4IV ACGT 5 cut(s) 535, 1490, 1767, 2947, 3835
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 181, 190, 223, 1112, 1121, 2576, 3101
HpySE526I ACGT 5 cut(s) 535, 1490, 1767, 2947, 3835
Hsp92I GRCGYC 3 cut(s) 89, 1086, 1915
Hsp92II CATG 8 cut(s) 654, 782, 1001, 1429, 1730, 2069, 2114, 3203
HspAI GCGC 4 cut(s) 327, 757, 2336, 3631
Kpn2I TCCGGA 1 cut(s) 2993
KpnI GGTACC 1 cut(s) 1035
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 1597
KspAI GTTAAC 1 cut(s) 1660
LguI GCTCTTC 1 cut(s) 3181
MaeII ACGT 5 cut(s) 535, 1490, 1767, 2947, 3835
MaeIII GTNAC 9 cut(s) 147, 503, 964, 1549, 2074, 2512, 2612, 2628, 3275
MbiI CCGCTC 2 cut(s) 339, 2273
MflI RGATCY 4 cut(s) 2882, 2990, 3050, 3619
MhlI GDGCHC 3 cut(s) 233, 240, 767
MlyI GAGTC 8 cut(s) 500, 574, 668, 1397, 3422, 3528, 3573, 3730
MmeI TCCRAC 3 cut(s) 153, 3770, 3788
Mph1103I ATGCAT 1 cut(s) 976
MreI CGCCGGCG 1 cut(s) 7
MroI TCCGGA 1 cut(s) 2993
MroNI GCCGGC 1 cut(s) 7
MroXI GAANNNNTTC 3 cut(s) 1862, 2203, 3706
MslI CAYNNNNRTG 5 cut(s) 1271, 1605, 1641, 2781, 3567
MspCI CTTAAG 1 cut(s) 2240
MspI CCGG 6 cut(s) 8, 25, 227, 233, 2763, 2994
MspR9I CCNGG 3 cut(s) 228, 233, 1410
Mva1269I GAATGC 1 cut(s) 3343
MvaI CCWGG 1 cut(s) 1410
MvnI CGCG 2 cut(s) 166, 200
MwoI GCNNNNNNNGC 8 cut(s) 172, 181, 190, 223, 1112, 1121, 2576, 3101
NaeI GCCGGC 1 cut(s) 9
NciI CCSGG 2 cut(s) 228, 233
NcoI CCATGG 1 cut(s) 1425
NdeI CATATG 4 cut(s) 2782, 3351, 3604, 3817
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 8 cut(s) 654, 782, 1001, 1429, 1730, 2069, 2114, 3203
NlaIV GGNNCC 8 cut(s) 29, 127, 231, 414, 628, 1033, 1396, 2761
NmeAIII GCCGAG 4 cut(s) 102, 195, 247, 3034
NmuCI GTSAC 5 cut(s) 147, 503, 2512, 2612, 3275
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 1 cut(s) 1730
NspV TTCGAA 2 cut(s) 1109, 2171
PagI TCATGA 2 cut(s) 778, 2110
PceI AGGCCT 1 cut(s) 2600
PciSI GCTCTTC 1 cut(s) 3181
PctI GAATGC 1 cut(s) 3343
PdiI GCCGGC 1 cut(s) 9
PdmI GAANNNNTTC 3 cut(s) 1862, 2203, 3706
PleI GAGTC 8 cut(s) 500, 573, 668, 1397, 3422, 3528, 3573, 3729
PpsI GAGTC 8 cut(s) 500, 573, 668, 1397, 3422, 3528, 3573, 3729
Ppu21I YACGTR 1 cut(s) 2948
PshAI GACNNNNGTC 1 cut(s) 3138
PsiI TTATAA 1 cut(s) 1970
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 1408
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 1408
PspN4I GGNNCC 8 cut(s) 29, 127, 231, 414, 628, 1033, 1396, 2761
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 6 cut(s) 27, 134, 229, 230, 413, 1559
PstI CTGCAG 3 cut(s) 1891, 3094, 3140
PsuI RGATCY 4 cut(s) 2882, 2990, 3050, 3619
RsaI GTAC 6 cut(s) 991, 1033, 1981, 2420, 3258, 3612
RsaNI GTAC 6 cut(s) 990, 1032, 1980, 2419, 3257, 3611
RseI CAYNNNNRTG 5 cut(s) 1271, 1605, 1641, 2781, 3567
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 3181
Sau96I GGNCC 6 cut(s) 27, 134, 229, 230, 413, 1559
ScaI AGTACT 2 cut(s) 991, 3612
SchI GAGTC 8 cut(s) 500, 574, 668, 1397, 3422, 3528, 3573, 3730
ScrFI CCNGG 3 cut(s) 228, 233, 1410
SduI GDGCHC 3 cut(s) 233, 240, 767
SfcI CTRYAG 9 cut(s) 601, 1452, 1887, 2260, 2559, 3090, 3136, 3697, 3757
SfuI TTCGAA 2 cut(s) 1109, 2171
SgrAI CRCCGGYG 1 cut(s) 7
SinI GGWCC 4 cut(s) 27, 134, 413, 1559
SmiMI CAYNNNNRTG 5 cut(s) 1271, 1605, 1641, 2781, 3567
SmlI CTYRAG 5 cut(s) 383, 2240, 2861, 3430, 3554
SmoI CTYRAG 5 cut(s) 383, 2240, 2861, 3430, 3554
SpeI ACTAGT 1 cut(s) 272
SseBI AGGCCT 1 cut(s) 2600
SspI AATATT 3 cut(s) 542, 1653, 3046
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 2600
StyD4I CCNGG 3 cut(s) 226, 231, 1408
StyI CCWWGG 5 cut(s) 1374, 1390, 1425, 1510, 2580
TaiI ACGT 5 cut(s) 538, 1493, 1770, 2950, 3838
TaqI TCGA 8 cut(s) 206, 299, 702, 1109, 2171, 2269, 2464, 3346
TaqII GACCGA 2 cut(s) 150, 430
TatI WGTACW 4 cut(s) 989, 1979, 3256, 3610
TauI GCSGC 6 cut(s) 14, 17, 169, 2065, 2276, 2570
TscAI CASTG 5 cut(s) 934, 1042, 1522, 2746, 3018
TseFI GTSAC 5 cut(s) 147, 503, 2512, 2612, 3275
Tsp45I GTSAC 5 cut(s) 147, 503, 2512, 2612, 3275
TspGWI ACGGA 3 cut(s) 833, 1188, 2296
TspRI CASTG 5 cut(s) 934, 1042, 1522, 2746, 3018
Vha464I CTTAAG 1 cut(s) 2240
VpaK11BI GGWCC 4 cut(s) 27, 134, 413, 1559
XapI RAATTY 7 cut(s) 454, 694, 740, 1627, 2183, 2377, 2438
XbaI TCTAGA 2 cut(s) 1168, 3182
XceI RCATGY 1 cut(s) 1730
XcmI CCANNNNNNNNNTGG 1 cut(s) 1004
XmaJI CCTAGG 1 cut(s) 1510
XmiI GTMKAC 1 cut(s) 1918
XmnI GAANNNNTTC 3 cut(s) 1862, 2203, 3706
ZrmI AGTACT 2 cut(s) 991, 3612
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.