RLG00000023251

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003093
Physical Location & Seq
Forward (+)
20588135 .. 20596087
7953 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023251

Sequence Viewer

Length: 3777 bp
ATGTCGCCGGCGGCGGCGGACGTCCGGCCCCCGATTACCTCTAAACCCTCCGGCAATCCTCAAAACCCTAAACCTTCATCGGAGGAGACGCCTTCCTCTAACCCCTTCAATTTCAATGCTGTTGGTTCCGACGGTCCGTCGGGTCCAAATGTGCCGTCACCATCAGCTCGGCGGCGGCCGAGGTTGGGGAAGCAGAGGAGGAGGCTGCATGCGAATGCGAGGTCGAGAACTGGGCCGGCTGAGACGGCCGGGCCGGAGTTCAACCCGTTTTGCTCGAATCATTCAGCACCGAATTTGAGTGCCGAAAATGGAGAATCGGCAGAATGTGTTGAGAAACTCGGTGATTTTGCGTGTGGTTCAGAGGTAGGACAGAGTGAATATTGTGAAAATGAGGGAAAGTCAGGTAATGACTCTAGTGGTATTGGTTTTGTGTTTGGTTCCGGTTGGTTTAATTCAAGTTCAGATTTGAATTCAGGAAAGAATGGCTCTAGTGGAAATGTGGAAAAATTGGTTGATGAAGTTGGCAGGAAGATGAGAGTAGAGTGTGACACCGAGTTTGAGAAAGTGAAAGTTAATTCTTTCGAATTTCATACAGAGGAGCGTTGGAGCTCAGACAAGGTTCATGATGAGGGCATTTTTGTATTTGGAAGCAGCGGTAAGAAAGGTTCCAGTTTGACGGATTCCAAGGTAGCTAATTGTCAAGATGAGGTGAAATCAAGTGATGAAGGAGATTGCAAGTCCAATTCAAACAGTCGGAATGATAATCTGAGTTCTGATTTCAGTAGCAAGTGTAATCTTGTATCTGAAACTAGTGAGGTAACTTGTGGAGATAATCATAAAAGTACTGACCATGTTGTATTTGGAAGCGAAAGTAACACGGCTGGTACCACCAGTGGGATTTCTAGTTCCACTGAGTTTACATTTCAGGCAGGACTTGGTGATAGTGATGATGCAGGCCAGTTTCCTGAGTGTCAAATTAATAAACCCAATGCAGCTGCTGGCCCATGTTCATTCTCATCAATTGGTCTTGGCATCCAACTAAATGGTTGTGTTTCTGAAGCAGCTTCTGCGGGTGGAGTAAAAGGTAAAGATGAAAGTAGCTTTACTAGCTCTCCAGATGGATTTGGAGTGTGCTTTGAGGACTTCAACACTCCATTGCGGTTTCCTGCTTGCCTCAAAGAAAATCTATTTCCTGAAATAAATAAATCAAACATCAGTATGAAGGGTAGATCGGTTAGGGACAAGAGATCAAGGAAAACAAGGGGAAGGTTGAAGAAACCTTCTCTGAGTAAGCAATGGCCAGCACAAGATAATGTTCCCAAGGAAAGTAGTTCCCAAGAAAACCCAGTCTCACCTGGATGTGGCTCACCCATGGATTTCTCTCCATATGAGGAAACTACAGTTGCTGATCCACATTTGAGGGAATCTTCTACAATGTCTAATGACACCTTCCACCTAGTCAGTAATAGTGCACCTTGTGGCTCCGATGCAACAGTTCCCATTGGACCGAATGGTGATGAGGGAGAATTAGCCGCTAAAGGTGATCAGATATATGGTAAGGCCACTGACGAGAATTCTAGGTATCATGGTGAAAGAATATTTGTTGACAATTGTTCCTCCAAAGGGTCTGGTTCTGGAGCTGAAACTCCCTGCTTTAGCTCCAATGGTATCAGTCATGCTGGTAAGGATTCAGAAGACGTTAGAGCTGATAATGGTGTGAATTTAGAAAGTCAAGAACAGGATTGTGGAACACCGTGTTTTGCTTCTGGTTTGGAAAATATGGAGAGGAAAACATTTACCTTTGTTGCATCATCTTCTACAGAAGGAAGTCCAATAGTGGCAAGGCGTCTTCTGCGCAGAGCGAAAAATAAGAAGAAAGTTGGTTCTAATACGTTTGTTATAACTCCAAGTCCAAATGTTCAGTTTGGAGGTTCTGACCAATTTACACCCCACTCTAGCACACCATTGTCTTTTGATGCGGTAGGAAAATCTGAGTCCCATGAACAAGTAACGAAAGTGCATATCCCTTCTACTGAGGCTACTCATGAGACTTGTGAGAAGTGGCGAATTAGGGGCAACAAAGCTTATAGGAACAAAGACTTTTCAAAAGCTGAAGACTTTTACACACAGGGAATAGTTTCTGTACCTTCAAGTGAAAGATCGGGAAGCTGCCTTAAGCCTCTTTTACTATGCTATAGCAATCGTGCAGCATCAAGGCTGTATATGGGAAAGATAAGGGAAGCTTTAGGGGACTGCATGATGGCCACTGCACTAGACCCCAACTTTCTCAAAGTTCAAATGAGAGCTGCAAATTTCCATCTTCTTCTCGGCGAAGTTGAAAATGCCCAACGGTACTTTAATAATTGCTTGGAATCCGGAACTGGTGTGTGTTTGGATCGGAGAATTGTAATTGATTCTGCTGATGGCATACAAAAGGCTCAGAAAGTGGCTGAGTATACGAATCGCTCTGCCAAGCTTCTGGAACAGAGAACTACAGATGCAGCTCTCAGTGCCTTGGAAATTGTTTCTGAGGCCTTGTCTATTAGTGAATACTCGGAAAAGTTACTTGAAATGAAGGCAGAGGCTTTGTGTTTGTTGAGGAGATATGAAGAGGCAATTCAACTGTGTGAACAGAGTATGTATTTTGCTGAGAAGAACTTTTCTTCGCTGAACAGTGTAGTAAATATGGATACTGCTGGATGTGAAGATCATCCATATGCTAGGTTGTGGAGGTGGTTCTTCATATCCAAATCTTACTTTCATTTGGGAAGGTTTCAGGCAGCTCTTGATTTACTTGAGAAGCTAGAGCAAGTGGGATCTATGAAAGACAGGTATGCGAGTAAGAATCTGGAATCATCAATTTCATTAGCTGTCACCATACGTGAACTGTTAAGCCACAAGGATGCAGGGAATGAAGCATTTAGATCAGGAAGATATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTCGCAATATTGGATCTCGGCCATTTTCTGCAATCTGTTTGTGCAACCGTGCTGCAGCACACCAAGCTTTGGGTCAAATTACTGATGCCATTGCTGACTGCAGTCTTGCAATAGCTCTTGATGGACATTATGTTAAGGCAGTTTCTAGAAGAGCTACCTTACATGAGATGATACGGGACTATGGACAGGCTGCTAGCGATCTCCAAAGACTTATTTCTGTACTGGAAAACCAATCTGGTGACAAGGCCAAAGAACCTACCTTACAAGGTAGCTCCAATGGCCGTTCACAAGAATTACGAAACTCTTATCGACGTATGCCTTTAATGGAAGAAGAAGCTAAGAAAGGAACCCCCTTGAATTTTTACCTCATTCTGGGAATTAAATCATCTGACACAAGTTCTGACATTAAGAAGGCATACCGTAAGGCTGCTCTCAAGCATCATCCCGACAAGGCTGGTCAGTTCTTGGCAAGAAGTGAGAGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTCTCGAGGTTCACAAGGATGCTGACAGACTCTTTAAAATGATCGGCGAGGCATATGCAGTACTTTCAGATCCCAACAAGCGCTCAGAATATGATCTTGAGGAAGACATAAGAAAATCAGCAAAGGAAAGCAAGGAACGTGGCGTCTACAGAAAGTCTTCAGATTTTCAGAGTCCTAGACGCAGCAGCTATAGAAAACCAGATTTTTATAGCTCTCCATTCGAGAGAAGCTCATATTGTCGGAACAGTAGGGAGAGTTGGAAGACATATGGAGATTCGTATTCACGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

1259

Amino Acids

137.67

Weight (kDa)

6.17

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1110 - 1142 1.7e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1901
AatII GACGTC 1 cut(s) 24
Acc16I TGCGCA 1 cut(s) 1856
Acc65I GGTACC 1 cut(s) 884
AccB1I GGYRCC 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 3040
AccI GTMKAC 2 cut(s) 2456, 3630
AccIII TCCGGA 1 cut(s) 2375
AclWI GGATC 5 cut(s) 1403, 2403, 2824, 2992, 3548
AcoI YGGCCR 6 cut(s) 176, 246, 1298, 2262, 2990, 3250
AcsI RAATTY 7 cut(s) 292, 469, 584, 1573, 1720, 2311, 3328
AcuI CTGAAG 3 cut(s) 1077, 2133, 3627
AcyI GRCGYC 4 cut(s) 21, 89, 1846, 3627
AdeI CACNNNGTG 1 cut(s) 1478
AfaI GTAC 6 cut(s) 844, 886, 2145, 2354, 3192, 3546
AfeI AGCGCT 1 cut(s) 3566
AfiI CCNNNNNNNGG 6 cut(s) 185, 894, 1361, 1623, 3040, 3343
AflII CTTAAG 1 cut(s) 2174
AhdI GACNNNNNGTC 1 cut(s) 136
AhlI ACTAGT 1 cut(s) 809
AjnI CCWGG 1 cut(s) 1354
AloI GAACNNNNNNTCC 2 cut(s) 889, 921
Alw21I GWGCWC 2 cut(s) 611, 1474
Alw26I GTCTC 4 cut(s) 80, 236, 1354, 2042
Alw44I GTGCAC 1 cut(s) 1470
AlwI GGATC 5 cut(s) 1403, 2403, 2824, 2992, 3548
AlwNI CAGNNNCTG 3 cut(s) 998, 1067, 1406
Ama87I CYCGRG 1 cut(s) 3488
Aor13HI TCCGGA 1 cut(s) 2375
Aor51HI AGCGCT 1 cut(s) 3566
ApaLI GTGCAC 1 cut(s) 1470
ApoI RAATTY 7 cut(s) 292, 469, 584, 1573, 1720, 2311, 3328
ArsI GACNNNNNNTTYG 6 cut(s) 140, 172, 206, 238, 539, 571
AseI ATTAAT 1 cut(s) 978
Asp700I GAANNNNTTC 2 cut(s) 2137, 3640
Asp718I GGTACC 1 cut(s) 884
AspLEI GCGC 2 cut(s) 1857, 3567
AspS9I GGNCC 7 cut(s) 27, 134, 143, 233, 251, 1001, 1505
AsuC2I CCSGG 1 cut(s) 250
AsuII TTCGAA 1 cut(s) 582
AsuNHI GCTAGC 1 cut(s) 3164
AvaI CYCGRG 1 cut(s) 3488
AvaII GGWCC 3 cut(s) 134, 143, 1505
BaeGI GKGCMC 1 cut(s) 1474
BalI TGGCCA 2 cut(s) 1300, 2264
BanI GGYRCC 1 cut(s) 884
BanII GRGCYC 1 cut(s) 611
BbsI GAAGAC 6 cut(s) 1701, 1841, 2121, 3594, 3633, 3752
Bbv12I GWGCWC 2 cut(s) 611, 1474
BccI CCATC 7 cut(s) 169, 1112, 2254, 2325, 2417, 3086, 3765
BceAI ACGGC 4 cut(s) 139, 261, 894, 3237
BcgI CGANNNNNNTGC 2 cut(s) 3521, 3555
BciT130I CCWGG 1 cut(s) 1356
BciVI GTATCC 1 cut(s) 2683
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 4 cut(s) 80, 236, 1354, 2042
BcuI ACTAGT 1 cut(s) 809
BfmI CTRYAG 8 cut(s) 1398, 1818, 2194, 2493, 3024, 3070, 3631, 3673
BfoI RGCGCY 1 cut(s) 3568
BfrI CTTAAG 1 cut(s) 2174
BfuI GTATCC 1 cut(s) 2683
BmcAI AGTACT 2 cut(s) 844, 3546
Bme1390I CCNGG 2 cut(s) 250, 1356
Bme18I GGWCC 3 cut(s) 134, 143, 1505
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 3488
BmgT120I GGNCC 7 cut(s) 27, 134, 143, 233, 251, 1001, 1505
BmiI GGNNCC 8 cut(s) 29, 127, 144, 439, 667, 886, 1483, 3319
BmrFI CCNGG 2 cut(s) 250, 1356
BmrI ACTGGG 2 cut(s) 240, 1340
BmtI GCTAGC 1 cut(s) 3168
BmuI ACTGGG 2 cut(s) 240, 1340
BoxI GACNNNNGTC 1 cut(s) 3072
BpiI GAAGAC 6 cut(s) 1701, 1841, 2121, 3594, 3633, 3752
BplI GAGNNNNNCTC 2 cut(s) 3698, 3730
BpmI CTGGAG 2 cut(s) 1098, 1656
Bpu14I TTCGAA 1 cut(s) 582
BpuEI CTTGAG 3 cut(s) 2816, 3389, 3602
BpuMI CCSGG 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 2882
BsaHI GRCGYC 4 cut(s) 21, 89, 1846, 3627
BsaJI CCNNGG 5 cut(s) 179, 684, 1320, 1371, 2514
BsaWI WCCGGW 2 cut(s) 440, 2375
BsaXI ACNNNNNCTCC 2 cut(s) 1096, 1126
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 894, 1361, 1623, 3040, 3343
Bse118I RCCGGY 2 cut(s) 7, 235
Bse1I ACTGG 7 cut(s) 235, 669, 891, 958, 1346, 2386, 3198
Bse3DI GCAATG 3 cut(s) 1154, 1301, 3060
BseAI TCCGGA 1 cut(s) 2375
BseBI CCWGG 1 cut(s) 1356
BseDI CCNNGG 5 cut(s) 179, 684, 1320, 1371, 2514
BseGI GGATG 7 cut(s) 1032, 1364, 2705, 2710, 2908, 3412, 3508
BseLI CCNNNNNNNGG 6 cut(s) 185, 894, 1361, 1623, 3040, 3343
BseMI GCAATG 3 cut(s) 1154, 1301, 3060
BseNI ACTGG 7 cut(s) 235, 669, 891, 958, 1346, 2386, 3198
BseRI GAGGAG 5 cut(s) 98, 211, 214, 611, 2614
BseSI GKGCMC 1 cut(s) 1474
BseX3I CGGCCG 2 cut(s) 176, 246
BsgI GTGCAG 2 cut(s) 2226, 2253
Bsh1285I CGRYCG 2 cut(s) 179, 249
BshNI GGYRCC 1 cut(s) 884
BsiEI CGRYCG 2 cut(s) 179, 249
BsiHKAI GWGCWC 2 cut(s) 611, 1474
BsiHKCI CYCGRG 1 cut(s) 3488
BsiSI CCGG 8 cut(s) 8, 25, 51, 236, 249, 254, 441, 2376
BslFI GGGAC 4 cut(s) 1253, 1981, 2264, 3161
BslI CCNNNNNNNGG 6 cut(s) 185, 894, 1361, 1623, 3040, 3343
BsmAI GTCTC 4 cut(s) 80, 236, 1354, 2042
BsmBI CGTCTC 2 cut(s) 80, 236
BsmFI GGGAC 4 cut(s) 1253, 1981, 2264, 3161
BsmI GAATGC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 3488
Bsp119I TTCGAA 1 cut(s) 582
Bsp1286I GDGCHC 2 cut(s) 611, 1474
Bsp13I TCCGGA 1 cut(s) 2375
Bsp19I CCATGG 1 cut(s) 1371
BspEI TCCGGA 1 cut(s) 2375
BspHI TCATGA 2 cut(s) 622, 2044
BspLI GGNNCC 8 cut(s) 29, 127, 144, 439, 667, 886, 1483, 3319
BspMAI CTGCAG 2 cut(s) 3028, 3074
BspOI GCTAGC 1 cut(s) 3168
BspPI GGATC 5 cut(s) 1403, 2403, 2824, 2992, 3548
BspQI GCTCTTC 1 cut(s) 3115
BspT104I TTCGAA 1 cut(s) 582
BspT107I GGYRCC 1 cut(s) 884
BspTI CTTAAG 1 cut(s) 2174
BsrDI GCAATG 3 cut(s) 1154, 1301, 3060
BsrFI RCCGGY 2 cut(s) 7, 235
BsrI ACTGG 7 cut(s) 235, 669, 891, 958, 1346, 2386, 3198
BssAI RCCGGY 2 cut(s) 7, 235
BssECI CCNNGG 5 cut(s) 179, 684, 1320, 1371, 2514
BssNAI GTATAC 1 cut(s) 2457
BssNI GRCGYC 4 cut(s) 21, 89, 1846, 3627
BssT1I CCWWGG 4 cut(s) 684, 1320, 1371, 2514
Bst1107I GTATAC 1 cut(s) 2457
Bst2UI CCWGG 1 cut(s) 1356
Bst6I CTCTTC 2 cut(s) 2604, 3115
BstACI GRCGYC 4 cut(s) 21, 89, 1846, 3627
BstAFI CTTAAG 1 cut(s) 2174
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstBAI YACGTR 1 cut(s) 2882
BstBI TTCGAA 1 cut(s) 582
BstC8I GCNNGC 8 cut(s) 9, 210, 237, 955, 1000, 1171, 1302, 3166
BstDSI CCRYGG 1 cut(s) 1371
BstF5I GGATG 7 cut(s) 1032, 1364, 2705, 2710, 2908, 3412, 3508
BstH2I RGCGCY 1 cut(s) 3568
BstHHI GCGC 2 cut(s) 1857, 3567
BstMAI GTCTC 4 cut(s) 80, 236, 1354, 2042
BstMCI CGRYCG 2 cut(s) 179, 249
BstMWI GCNNNNNNNGC 7 cut(s) 245, 1067, 1107, 1852, 2510, 3035, 3249
BstNI CCWGG 1 cut(s) 1356
BstNSI RCATGY 1 cut(s) 212
BstPAI GACNNNNGTC 1 cut(s) 3072
BstSCI CCNGG 2 cut(s) 248, 1354
BstSFI CTRYAG 8 cut(s) 1398, 1818, 2194, 2493, 3024, 3070, 3631, 3673
BstSLI GKGCMC 1 cut(s) 1474
BstV2I GAAGAC 6 cut(s) 1701, 1841, 2121, 3594, 3633, 3752
BstX2I RGATCY 3 cut(s) 2816, 2984, 3553
BstXI CCANNNNNNTGG 2 cut(s) 1043, 2479
BstYI RGATCY 3 cut(s) 2816, 2984, 3553
BstZ17I GTATAC 1 cut(s) 2457
BstZI CGGCCG 2 cut(s) 176, 246
BsuI GTATCC 1 cut(s) 2683
BtgI CCRYGG 1 cut(s) 1371
BtsCI GGATG 7 cut(s) 1032, 1364, 2705, 2710, 2908, 3412, 3508
BtsI GCAGTG 2 cut(s) 2265, 2952
BtsIMutI CAGTG 7 cut(s) 898, 909, 1563, 2265, 2515, 2680, 2952
Cac8I GCNNGC 8 cut(s) 9, 210, 237, 955, 1000, 1171, 1302, 3166
CaiI CAGNNNCTG 3 cut(s) 998, 1067, 1406
CciI TCATGA 2 cut(s) 622, 2044
CfoI GCGC 2 cut(s) 1857, 3567
Cfr10I RCCGGY 2 cut(s) 7, 235
Cfr13I GGNCC 7 cut(s) 27, 134, 143, 233, 251, 1001, 1505
CpoI CGGWCCG 1 cut(s) 134
CseI GACGC 4 cut(s) 97, 1835, 3616, 3672
Csp6I GTAC 6 cut(s) 843, 885, 2144, 2353, 3191, 3545
CspCI CAANNNNNGTGG 2 cut(s) 3604, 3639
CspI CGGWCCG 1 cut(s) 134
CviQI GTAC 6 cut(s) 843, 885, 2144, 2353, 3191, 3545
DraI TTTAAA 1 cut(s) 3520
DraIII CACNNNGTG 1 cut(s) 1478
DriI GACNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 6 cut(s) 176, 246, 1298, 2262, 2990, 3250
EagI CGGCCG 2 cut(s) 176, 246
Eam1104I CTCTTC 2 cut(s) 2604, 3115
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 2 cut(s) 2604, 3115
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 609
EclXI CGGCCG 2 cut(s) 176, 246
Eco130I CCWWGG 4 cut(s) 684, 1320, 1371, 2514
Eco147I AGGCCT 1 cut(s) 2534
Eco24I GRGCYC 1 cut(s) 611
Eco47I GGWCC 3 cut(s) 134, 143, 1505
Eco47III AGCGCT 1 cut(s) 3566
Eco52I CGGCCG 2 cut(s) 176, 246
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 3 cut(s) 1077, 2133, 3627
Eco88I CYCGRG 1 cut(s) 3488
EcoICRI GAGCTC 1 cut(s) 609
EcoRI GAATTC 2 cut(s) 469, 1573
EcoRII CCWGG 1 cut(s) 1354
EcoT14I CCWWGG 4 cut(s) 684, 1320, 1371, 2514
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 4 cut(s) 684, 1320, 1371, 2514
Esp3I CGTCTC 2 cut(s) 80, 236
FalI AAGNNNNNCTT 2 cut(s) 2227, 2259
FaqI GGGAC 4 cut(s) 1253, 1981, 2264, 3161
FauI CCCGC 1 cut(s) 1063
FauNDI CATATG 4 cut(s) 1387, 2716, 3538, 3751
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 2 cut(s) 2456, 3630
FokI GGATG 7 cut(s) 1019, 1371, 2697, 2712, 2915, 3399, 3515
FriOI GRGCYC 1 cut(s) 611
FspI TGCGCA 1 cut(s) 1856
GlaI GCGC 2 cut(s) 1856, 3566
GsuI CTGGAG 2 cut(s) 1098, 1656
HaeII RGCGCY 1 cut(s) 3568
HapII CCGG 8 cut(s) 8, 25, 51, 236, 249, 254, 441, 2376
HgaI GACGC 4 cut(s) 97, 1835, 3616, 3672
HhaI GCGC 2 cut(s) 1857, 3567
Hin1I GRCGYC 4 cut(s) 21, 89, 1846, 3627
Hin6I GCGC 2 cut(s) 1855, 3565
HinP1I GCGC 2 cut(s) 1855, 3565
HincII GTYRAC 1 cut(s) 1606
HindII GTYRAC 1 cut(s) 1606
HindIII AAGCTT 4 cut(s) 2082, 2241, 2474, 3036
HpaII CCGG 8 cut(s) 8, 25, 51, 236, 249, 254, 441, 2376
Hpy166II GTNNAC 9 cut(s) 918, 1472, 1606, 2457, 2630, 2885, 3257, 3496, 3631
Hpy8I GTNNAC 9 cut(s) 918, 1472, 1606, 2457, 2630, 2885, 3257, 3496, 3631
Hpy99I CGWCG 3 cut(s) 134, 142, 3285
HpyCH4IV ACGT 6 cut(s) 21, 1698, 1892, 2881, 3283, 3622
HpyF10VI GCNNNNNNNGC 7 cut(s) 245, 1067, 1107, 1852, 2510, 3035, 3249
HpySE526I ACGT 6 cut(s) 21, 1698, 1892, 2881, 3283, 3622
Hsp92I GRCGYC 4 cut(s) 21, 89, 1846, 3627
HspAI GCGC 2 cut(s) 1855, 3565
Kpn2I TCCGGA 1 cut(s) 2375
KpnI GGTACC 1 cut(s) 888
KroI GCCGGC 2 cut(s) 7, 235
KroNI GCCGGC 2 cut(s) 9, 237
Ksp22I TGATCA 1 cut(s) 1543
LguI GCTCTTC 1 cut(s) 3115
LmnI GCTCC 7 cut(s) 598, 606, 1487, 1637, 1664, 2950, 3248
MaeII ACGT 6 cut(s) 21, 1698, 1892, 2881, 3283, 3622
MaeIII GTNAC 8 cut(s) 156, 545, 817, 872, 2008, 2562, 2872, 3209
MfeI CAATTG 2 cut(s) 1020, 1609
MflI RGATCY 3 cut(s) 2816, 2984, 3553
MhlI GDGCHC 2 cut(s) 611, 1474
MlsI TGGCCA 2 cut(s) 1300, 2264
MluNI TGGCCA 2 cut(s) 1300, 2264
MlyI GAGTC 4 cut(s) 404, 2003, 3507, 3664
MmeI TCCRAC 6 cut(s) 153, 584, 734, 1060, 3704, 3722
Mox20I TGGCCA 2 cut(s) 1300, 2264
MreI CGCCGGCG 1 cut(s) 7
MroI TCCGGA 1 cut(s) 2375
MroNI GCCGGC 2 cut(s) 7, 235
MroXI GAANNNNTTC 2 cut(s) 2137, 3640
MscI TGGCCA 2 cut(s) 1300, 2264
MslI CAYNNNNRTG 6 cut(s) 213, 1217, 1357, 2715, 2901, 3501
Msp20I TGGCCA 2 cut(s) 1300, 2264
MspA1I CMGCKG 2 cut(s) 654, 995
MspCI CTTAAG 1 cut(s) 2174
MspI CCGG 8 cut(s) 8, 25, 51, 236, 249, 254, 441, 2376
MspR9I CCNGG 2 cut(s) 250, 1356
MunI CAATTG 2 cut(s) 1020, 1609
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 1356
MwoI GCNNNNNNNGC 7 cut(s) 245, 1067, 1107, 1852, 2510, 3035, 3249
NaeI GCCGGC 2 cut(s) 9, 237
NciI CCSGG 1 cut(s) 250
NcoI CCATGG 1 cut(s) 1371
NdeI CATATG 4 cut(s) 1387, 2716, 3538, 3751
NgoMIV GCCGGC 2 cut(s) 7, 235
NheI GCTAGC 1 cut(s) 3164
NlaIV GGNNCC 8 cut(s) 29, 127, 144, 439, 667, 886, 1483, 3319
NmeAIII GCCGAG 4 cut(s) 148, 204, 2307, 2968
NmuCI GTSAC 4 cut(s) 156, 545, 2872, 3209
NsbI TGCGCA 1 cut(s) 1856
NspI RCATGY 1 cut(s) 212
NspV TTCGAA 1 cut(s) 582
PaeI GCATGC 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 3488
PagI TCATGA 2 cut(s) 622, 2044
PceI AGGCCT 1 cut(s) 2534
PciSI GCTCTTC 1 cut(s) 3115
PctI GAATGC 1 cut(s) 220
PdiI GCCGGC 2 cut(s) 9, 237
PdmI GAANNNNTTC 2 cut(s) 2137, 3640
PflMI CCANNNNNTGG 1 cut(s) 3040
PleI GAGTC 4 cut(s) 404, 2002, 3507, 3663
PpsI GAGTC 4 cut(s) 404, 2002, 3507, 3663
Ppu21I YACGTR 1 cut(s) 2882
PshAI GACNNNNGTC 1 cut(s) 3072
PshBI ATTAAT 1 cut(s) 978
PsiI TTATAA 1 cut(s) 1901
Psp124BI GAGCTC 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 1354
PspGI CCWGG 1 cut(s) 1354
PspN4I GGNNCC 8 cut(s) 29, 127, 144, 439, 667, 886, 1483, 3319
PspPI GGNCC 7 cut(s) 27, 134, 143, 233, 251, 1001, 1505
PsrI GAACNNNNNNTAC 4 cut(s) 649, 681, 3216, 3248
PstI CTGCAG 2 cut(s) 3028, 3074
PstNI CAGNNNCTG 3 cut(s) 998, 1067, 1406
PsuI RGATCY 3 cut(s) 2816, 2984, 3553
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 6 cut(s) 844, 886, 2145, 2354, 3192, 3546
RsaNI GTAC 6 cut(s) 843, 885, 2144, 2353, 3191, 3545
RseI CAYNNNNRTG 6 cut(s) 213, 1217, 1357, 2715, 2901, 3501
Rsr2I CGGWCCG 1 cut(s) 134
RsrII CGGWCCG 1 cut(s) 134
SacI GAGCTC 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 3115
Sau96I GGNCC 7 cut(s) 27, 134, 143, 233, 251, 1001, 1505
ScaI AGTACT 2 cut(s) 844, 3546
SchI GAGTC 4 cut(s) 404, 2003, 3507, 3664
ScrFI CCNGG 2 cut(s) 250, 1356
SduI GDGCHC 2 cut(s) 611, 1474
SfcI CTRYAG 8 cut(s) 1398, 1818, 2194, 2493, 3024, 3070, 3631, 3673
Sfr274I CTCGAG 1 cut(s) 3488
SfuI TTCGAA 1 cut(s) 582
SgrAI CRCCGGYG 1 cut(s) 7
SinI GGWCC 3 cut(s) 134, 143, 1505
SlaI CTCGAG 1 cut(s) 3488
SmiMI CAYNNNNRTG 6 cut(s) 213, 1217, 1357, 2715, 2901, 3501
SmlI CTYRAG 5 cut(s) 2174, 2795, 3404, 3488, 3581
SmoI CTYRAG 5 cut(s) 2174, 2795, 3404, 3488, 3581
SpeI ACTAGT 1 cut(s) 809
SphI GCATGC 1 cut(s) 212
SseBI AGGCCT 1 cut(s) 2534
SspI AATATT 3 cut(s) 380, 1599, 2980
SstI GAGCTC 1 cut(s) 611
StuI AGGCCT 1 cut(s) 2534
StyD4I CCNGG 2 cut(s) 248, 1354
StyI CCWWGG 4 cut(s) 684, 1320, 1371, 2514
TaiI ACGT 6 cut(s) 24, 1701, 1895, 2884, 3286, 3625
TaqI TCGA 6 cut(s) 224, 275, 582, 3280, 3489, 3705
TaqII GACCGA 1 cut(s) 1522
TatI WGTACW 3 cut(s) 842, 3190, 3544
TauI GCSGC 5 cut(s) 14, 17, 175, 178, 1535
TscAI CASTG 7 cut(s) 898, 916, 1570, 2272, 2515, 2680, 2952
TseFI GTSAC 4 cut(s) 156, 545, 2872, 3209
Tsp45I GTSAC 4 cut(s) 156, 545, 2872, 3209
TspGWI ACGGA 2 cut(s) 126, 692
TspRI CASTG 7 cut(s) 898, 916, 1570, 2272, 2515, 2680, 2952
Van91I CCANNNNNTGG 1 cut(s) 3040
Vha464I CTTAAG 1 cut(s) 2174
VneI GTGCAC 1 cut(s) 1470
VpaK11BI GGWCC 3 cut(s) 134, 143, 1505
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 7 cut(s) 292, 469, 584, 1573, 1720, 2311, 3328
XbaI TCTAGA 1 cut(s) 3116
XceI RCATGY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 3488
XmiI GTMKAC 2 cut(s) 2456, 3630
XmnI GAANNNNTTC 2 cut(s) 2137, 3640
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 2 cut(s) 844, 3546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.