MD12G1083200.v1.1

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003093
Physical Location & Seq
Reverse (-)
10088157 .. 10102635
14479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1083200.v1.1.491

Sequence Viewer

Length: 4119 bp
ATGTCGCCGGCGGCAGTTGATGTCCGGCCTCCGGTCACCTCTCCGCCGCCCAAACCCTCATCTGCTCCTCAAAATTCTGCTCCTGCGTCCGACATGCCTTCCTCTTCTACTTTCAATCTCGGTGCTTCAGACGGCAATGGGAGTAAGTGTCAGTTCGGTCCGAGTGTGCCGTCGCGATCGGCTCGGTCGCGGCCGAGGTTGGTGAAGCTGAGGAAGCAGCATTCGAGGTCCAGAACTGGGTCGGCCGAGAGCGGGCCGGGGGTCAATCCGTTTTGCTCGGTAAGTGATGCTACTAGAGCTTCTAATGGGTTTAATTTTTCGACTGGCGATTGTGGGGGTGTGGGTTTTGTGTTTGGTGCTAAGAGTGGGGTTGAGAATTTGGATAATGGGGATGGAGAGAGTGATGGAATAGTGAGAAATGTGGATAATGCGGAAAGAGGGAGTGATGGGGTAGCGGGAACTTCGAGTTGTGATGATAATTTGGATAAAGGGGAAGGAGTGAAAACTTTGAATTGTGATGAGAGGGGGCAGATGGATGTCGGAAATGGAAGAGAGTTTGATAAGGTTGAGAGTGATAGTTTTGAGTGCGGTGCAAAGAACAATGATCTGGGATCGCTTTTGTGCGGTGAGAAGAGAGAATTTTGTGAAAATGTGAGAGGAAAGATTTCTGAAGATAAGTGGAATGCAAAAACAGATACTGAAACAGAATGTCAGAAGGTTGATAACATGGATTTTGCTTTTGATTCTAATAGTAGTGATTTGGGTTTGCATTTGAATTTGGATTCAAATCCAGAAATGCAAGAGTGTGATGGAAATGTGGAGAAACCGAGCATGGATAATAGGGGGGAAATGAAATTAGAGAGTGAAGTAGAGTACAATGTGGGCTCTGCTCCTTTCGTGTTTTGTTCTAGTTTGATTTCTAATTTAAATTCAAATATGGAAAAGAAAAAACCTAGTGGAAATCTGGACAAGTTGGCTTCTGATGTTGGCAGAAAGCTGAAAGTAGAGAGTGAAACAGACTTAGAGAAAGTGGAAGCTGATCCTTTTGAATTTCATGCTGAGAAGAGTTGCATCTTAAATAAGGATCAAGAGAACGCCTTTTTTGTATTTAGAAGCAGCAGTTATACTGAATGCATGGAAGCCAAGTGTCAAGATACAATGAAACTGAGTAGTGAAAATTTAGGAGGTTTCAAGACAAATTCAGACAGTAGTCCTTTCTGTCAGAGTTCAGGAAGTCCTTATATTGCCTCTGAGAGAACTAATGAAGATAATCATGAAAATAGTGGCCAAAACCATTTCTTATTTGGAAGTGATGGTAACATGGAGGGTGCCACAATAGGACTTTCTAGTTCCAAAGATTTTAAATCTGCAGGAAGTGCTGAGAGTGTTGAGGCAGGCCAATTCACCAAATGTCAAGTCAATCATAATACACTCCCGAATATAGATGCAGCATCAGCAACATGTTCATTTTCATCAGTTGGTCTTGGCTTCCAACCCAAGGGTTGTGCTTCTGAAGCAGCTTCTGTGGATGGAGTAAAAAAGAAAGATGATGTTAGTTTTACTGGCACTACAGATGGATTTGGAGTGTGCCTTGAAGACTTTAAGAGGTCATTTTGCGATCCTTCCTGTCTCAGAGAAAATTTATTTCCTGAATTAAATAAAACTTCAGAATGTGGTGCGAAGGGTAGGTCCTTTAGGGACAAAAGATCAAGGAAACAAAGGGGAAAGTCAAAGAAACCTTCCATGAGTAAGCCATGGCCACGACAAGACCATGTGCCTAAGGAAAGTGGTTCCCAAGAAAACCCAGAACCTTCTGGATGCTACTCGCCCATGGATTTCTCTCCCTATGAGGAAACAAGAGTTGCTGATCCACATTCAAGGGAACATTCTGTGATATCTGCTGACCCCTGCGAAACAGTTCCTGTAGATCCCAAAGGTGAAGGTTTGGCTGCTACAGCAAGTGGATTAGGTTATACGGTTGATCAGATATGTAAAGAGCCCATTGAGAAGAATTCGACGTATCATGGTGAGGGAGGTTTTTTCGGTGATGGTTTGTGGAAAGGTTCTGATTCAGAAACAGCTAGAGCTGGTATTGGATTAACTATAGAGAAACAAGAAAGTGATTGTAGATCACCATTTTTTGCTTCGGGTTCCGAAAAGGATAAATGCTTTACGTTTTTGGCATCATCTTCTGTGCAAGGTAGTTCAATGATGGGTAAGCGCCAACAACGTAGAAAGAAAAACAAGATAAAAGTTGGTCATGCAACATTTATTATTACTCCAAGTTCAAATGTTGAGTTTGGATCTTCTGGCCTATTTACCCCTCACTCTAGCATGCCTTTATGTACGGAGGTAGTTGGTAAATCAGAAGCAAAAGAACAATTTAAGCAAGTGCATGTTTCTTCTAGTGTTGCTATTCATGAAACCTGTGAGAAATGGCGGATCAGGGGAAATGAAGCTTATAAAAATGGAGATCATTCAAAAGCTGAAGAATTTTACACACAGGGAATAATTTCTATACCTTCTAATGAAAGAGCAGGATGCTCTCTTAAGCCTCTTTTGCTATGCTATAGCAACCGTGCTGCAACAAGAATGTGCCTTGGAAGGATAAGGGAAGCTTTAGGGGACTGTGTGATGGCTACTGCACTAGATCCCAACTTTCTCAAAGCTCAAATGAGAGCTGCAAATTGCCATCTTCTGCTCGGTGAAGTCGAAAATGCAAAGCAGTACTTCAATAAGTGTTCCGAATCAGTAAACGGTGTATGTTTGGACCGAAGAATTGTAATAAATTCTGCTGATGGCCTACAAAAGATTCAGAAAGTGATTGAGTATACAAATCGTTCTATCAAGCTGCTGGACCAGAGAAATACTGATGCAGCTCTCACTGCATTAGAGCTAATTTCTGAGGCCTTGTCAGTTAGCTCATGCTCAGAAACATTACTTGAAATGAAAGCAGAGGCTTTGTGTTTGTTGAGGAGGTATGAAGAGGCAATTCGTCTGTGTGAGCAGAGTATGGTTTTTGCTGAAAGGAATTTTTCTGGACTTGAAATTTATCAAGTTAAGTTGTGGAGGTGGTTCTTCATATCCAAGTCTCACTTTCATTTGGGAAGGCTTGAGGCAGCCCTTGATTTACTTGAGAAGCTAGAAAAAGTGGGATCTACAAAAGAGATGTATGCAAGTAAGAAACTGGAATCATCCGTGTCATTGGCTGTCACAATACGTGAACTATTACGCCACAAGAATGCAGGCAATGAAGCATTTAGATCAGGAAGCTATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTAGCAATATGGGATCTCGTCCGTTTTCAGCTATCTGTTTGTGCAACCGCGCTGCAGCACACCAAGCTTTGGGTCAAATTACTGATGCCATTGCAGACTGCAGTCTTGCCATAGCTCTTGATGGCAATTATGTAAAGGCAGTTTCTAGAAGAGCCACCTTACATGAGATGATACGAGACTATGGACAAGCGGCTAGTGATCTCCAAAGACTTGTATCCATTCTAGAAAACCAATCTAATGACATGGCCAAAGAGTCTGGCTCACAAGATAGATCCAATGGTAGTGTAAAAGAATTACGCAATGCTCGTCGACGTATGCCTTTGATGGAAGAAGAAGCTAAAAAAGGAATCTCCTTGGACTTTTATCTTATTTTGGGAATTAAACCATCCGATGAGAGTTCTGAAATTAAGAAGGCTTACCGTAAGGCAGCATTGAAGCATCATCCTGACAAGGCTGGCCAGTTCTTAGCAAGGAGTGAGTGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTGCGGAGGTTCACAAGGATGCTGACAGGCTCTTTAAAATGATTGGAGAGGCATATGCAGTACTTTCAGACCCTACGAAGCGGTCACAGTATGATCTCGAGGAGGAGATGAGAAAAGTAGATATTGAATGCAAGGAAAGCAGCATCTATAGAAAAGCTTCAGGTTTTCAGAGTCCTGGCTGCAGCAGTTATAGAAGACCTGATTTCCGTAGCTCTCCATTTGAGAGAAGCTCTGGTGGTCGAAACTATGGCAGAGAGAGTTGGAGGACATATGGAAATTCGTATCCTCGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

1373

Amino Acids

150.37

Weight (kDa)

5.96

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1223 - 1255 6.5e-10 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2463
AccB1I GGYRCC 1 cut(s) 1328
AccB7I CCANNNNNTGG 1 cut(s) 3379
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 2 cut(s) 2831, 3619
AccII CGCG 3 cut(s) 175, 190, 3360
AcoI YGGCCR 6 cut(s) 191, 243, 1285, 1757, 3555, 3766
AcuI CTGAAG 6 cut(s) 111, 690, 1533, 1650, 2508, 3966
AfaI GTAC 4 cut(s) 875, 2347, 2729, 3885
AfiI CCNNNNNNNGG 5 cut(s) 31, 237, 252, 1498, 3379
AflII CTTAAG 1 cut(s) 2549
AflIII ACRYGT 1 cut(s) 1460
AjnI CCWGG 1 cut(s) 3997
Alw26I GTCTC 3 cut(s) 1634, 3096, 3480
AlwNI CAGNNNCTG 3 cut(s) 698, 1523, 1922
Ama87I CYCGRG 1 cut(s) 3920
ArsI GACNNNNNNTTYG 4 cut(s) 1401, 1433, 1673, 1705
Asp700I GAANNNNTTC 4 cut(s) 664, 1917, 2512, 3979
AspLEI GCGC 2 cut(s) 2223, 3362
AspS9I GGNCC 6 cut(s) 158, 228, 254, 1689, 2770, 2857
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 9 cut(s) 28, 214, 638, 1396, 1949, 2039, 2057, 2124, 2717
AvaI CYCGRG 1 cut(s) 3920
AvaII GGWCC 5 cut(s) 158, 228, 1689, 2770, 2857
AxyI CCTNAGG 1 cut(s) 1779
BalI TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
BanI GGYRCC 1 cut(s) 1328
BanII GRGCYC 2 cut(s) 887, 2001
BbsI GAAGAC 2 cut(s) 1602, 4024
BbvCI CCTCAGC 1 cut(s) 209
BceAI ACGGC 2 cut(s) 148, 154
BciT130I CCWGG 1 cut(s) 3999
BciVI GTATCC 2 cut(s) 3535, 4116
BclI TGATCA 1 cut(s) 1981
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 3 cut(s) 1634, 3096, 3480
BfoI RGCGCY 1 cut(s) 2224
BfrI CTTAAG 1 cut(s) 2549
BfuI GTATCC 2 cut(s) 3535, 4116
BmcAI AGTACT 2 cut(s) 2729, 3885
Bme1390I CCNGG 2 cut(s) 258, 3999
Bme18I GGWCC 5 cut(s) 158, 228, 1689, 2770, 2857
BmeT110I CYCGRG 1 cut(s) 3920
BmgT120I GGNCC 6 cut(s) 158, 228, 254, 1689, 2770, 2857
BmiI GGNNCC 3 cut(s) 1330, 1792, 2152
BmrFI CCNGG 2 cut(s) 258, 3999
BmrI ACTGGG 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 246
BoxI GACNNNNGTC 2 cut(s) 1209, 3411
BpiI GAAGAC 2 cut(s) 1602, 4024
BplI GAGNNNNNCTC 4 cut(s) 3554, 3586, 4037, 4069
Bpu10I CCTNAGC 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 3134, 3155
BpuMI CCSGG 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 3221
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 7 cut(s) 194, 257, 1497, 1754, 1830, 2599, 3663
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 3775, 3805
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 237, 252, 1498, 3379
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 5 cut(s) 241, 328, 1567, 3192, 3769
Bse21I CCTNAGG 1 cut(s) 1779
Bse3DI GCAATG 4 cut(s) 142, 3256, 3399, 3616
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 1 cut(s) 3999
BseDI CCNNGG 7 cut(s) 194, 257, 1497, 1754, 1830, 2599, 3663
BseGI GGATG 9 cut(s) 397, 541, 1534, 1823, 2546, 3194, 3695, 3751, 3847
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 5 cut(s) 31, 237, 252, 1498, 3379
BseMI GCAATG 4 cut(s) 142, 3256, 3399, 3616
BseMII CTCAG 8 cut(s) 200, 1050, 1157, 1242, 1371, 1645, 2895, 2943
BseNI ACTGG 5 cut(s) 241, 328, 1567, 3192, 3769
BseRI GAGGAG 4 cut(s) 57, 2989, 3938, 3941
BseX3I CGGCCG 2 cut(s) 191, 243
BsgI GTGCAG 1 cut(s) 2628
Bsh1236I CGCG 3 cut(s) 175, 190, 3360
Bsh1285I CGRYCG 4 cut(s) 179, 188, 194, 246
BshNI GGYRCC 1 cut(s) 1328
BsiEI CGRYCG 4 cut(s) 179, 188, 194, 246
BsiHKCI CYCGRG 1 cut(s) 3920
BsiSI CCGG 4 cut(s) 8, 25, 32, 257
BslFI GGGAC 2 cut(s) 1712, 2639
BslI CCNNNNNNNGG 5 cut(s) 31, 237, 252, 1498, 3379
BsmAI GTCTC 3 cut(s) 1634, 3096, 3480
BsmFI GGGAC 2 cut(s) 1712, 2639
BsmI GAATGC 5 cut(s) 220, 688, 1136, 3247, 3956
BsoBI CYCGRG 1 cut(s) 3920
Bsp1286I GDGCHC 2 cut(s) 887, 2001
Bsp19I CCATGG 2 cut(s) 1754, 1830
Bsp68I TCGCGA 1 cut(s) 175
BspCNI CTCAG 8 cut(s) 201, 1051, 1158, 1243, 1372, 1644, 2896, 2942
BspFNI CGCG 3 cut(s) 175, 190, 3360
BspHI TCATGA 2 cut(s) 1273, 2419
BspLI GGNNCC 3 cut(s) 1330, 1792, 2152
BspMAI CTGCAG 4 cut(s) 1372, 3367, 3413, 4007
BspQI GCTCTTC 1 cut(s) 3454
BspT107I GGYRCC 1 cut(s) 1328
BspTI CTTAAG 1 cut(s) 2549
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 4 cut(s) 142, 3256, 3399, 3616
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 5 cut(s) 241, 328, 1567, 3192, 3769
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 7 cut(s) 194, 257, 1497, 1754, 1830, 2599, 3663
BssNAI GTATAC 1 cut(s) 2832
BssT1I CCWWGG 5 cut(s) 1497, 1754, 1830, 2599, 3663
Bst1107I GTATAC 1 cut(s) 2832
Bst2UI CCWGG 1 cut(s) 3999
Bst4CI ACNGT 9 cut(s) 1208, 1918, 1978, 2579, 2630, 2759, 3301, 3731, 3912
Bst6I CTCTTC 6 cut(s) 109, 544, 626, 1058, 2979, 3454
BstAFI CTTAAG 1 cut(s) 2549
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstBAI YACGTR 1 cut(s) 3221
BstC8I GCNNGC 6 cut(s) 9, 254, 1396, 2336, 3247, 3766
BstDSI CCRYGG 2 cut(s) 1754, 1830
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 9 cut(s) 397, 541, 1534, 1823, 2546, 3194, 3695, 3751, 3847
BstFNI CGCG 3 cut(s) 175, 190, 3360
BstH2I RGCGCY 1 cut(s) 2224
BstHHI GCGC 2 cut(s) 2223, 3362
BstMAI GTCTC 3 cut(s) 1634, 3096, 3480
BstMCI CGRYCG 4 cut(s) 179, 188, 194, 246
BstNI CCWGG 1 cut(s) 3999
BstNSI RCATGY 4 cut(s) 97, 1464, 2338, 2399
BstPAI GACNNNNGTC 2 cut(s) 1209, 3411
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 256, 3997
BstUI CGCG 3 cut(s) 175, 190, 3360
BstV2I GAAGAC 2 cut(s) 1602, 4024
BstX2I RGATCY 6 cut(s) 1927, 2303, 2650, 3155, 3323, 3581
BstYI RGATCY 6 cut(s) 1927, 2303, 2650, 3155, 3323, 3581
BstZ17I GTATAC 1 cut(s) 2832
BstZI CGGCCG 2 cut(s) 191, 243
Bsu36I CCTNAGG 1 cut(s) 1779
BsuI GTATCC 2 cut(s) 3535, 4116
BtgI CCRYGG 2 cut(s) 1754, 1830
BtsCI GGATG 9 cut(s) 397, 541, 1534, 1823, 2546, 3194, 3695, 3751, 3847
BtsI GCAGTG 2 cut(s) 2883, 3291
BtsIMutI CAGTG 2 cut(s) 2883, 3291
BtuMI TCGCGA 1 cut(s) 175
Cac8I GCNNGC 6 cut(s) 9, 254, 1396, 2336, 3247, 3766
CaiI CAGNNNCTG 3 cut(s) 698, 1523, 1922
CciI TCATGA 2 cut(s) 1273, 2419
CfoI GCGC 2 cut(s) 2223, 3362
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 6 cut(s) 158, 228, 254, 1689, 2770, 2857
CpoI CGGWCCG 1 cut(s) 158
CseI GACGC 1 cut(s) 75
Csp6I GTAC 4 cut(s) 874, 2346, 2728, 3884
CspI CGGWCCG 1 cut(s) 158
CviQI GTAC 4 cut(s) 874, 2346, 2728, 3884
DraI TTTAAA 3 cut(s) 927, 1363, 3859
EaeI YGGCCR 6 cut(s) 191, 243, 1285, 1757, 3555, 3766
EagI CGGCCG 2 cut(s) 191, 243
Eam1104I CTCTTC 6 cut(s) 109, 544, 626, 1058, 2979, 3454
EarI CTCTTC 6 cut(s) 109, 544, 626, 1058, 2979, 3454
EciI GGCGGA 2 cut(s) 33, 2455
EclXI CGGCCG 2 cut(s) 191, 243
Eco130I CCWWGG 5 cut(s) 1497, 1754, 1830, 2599, 3663
Eco147I AGGCCT 1 cut(s) 2909
Eco24I GRGCYC 2 cut(s) 887, 2001
Eco32I GATATC 1 cut(s) 1896
Eco47I GGWCC 5 cut(s) 158, 228, 1689, 2770, 2857
Eco52I CGGCCG 2 cut(s) 191, 243
Eco57I CTGAAG 6 cut(s) 111, 690, 1533, 1650, 2508, 3966
Eco81I CCTNAGG 1 cut(s) 1779
Eco88I CYCGRG 1 cut(s) 3920
Eco91I GGTNACC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 1689
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 2011
EcoRII CCWGG 1 cut(s) 3997
EcoRV GATATC 1 cut(s) 1896
EcoT14I CCWWGG 5 cut(s) 1497, 1754, 1830, 2599, 3663
EcoT22I ATGCAT 1 cut(s) 1136
EcoT38I GRGCYC 2 cut(s) 887, 2001
ErhI CCWWGG 5 cut(s) 1497, 1754, 1830, 2599, 3663
FalI AAGNNNNNCTT 6 cut(s) 2602, 2634, 2714, 2746, 3080, 3112
FaqI GGGAC 2 cut(s) 1712, 2639
FauI CCCGC 2 cut(s) 245, 448
FauNDI CATATG 2 cut(s) 3877, 4093
FbaI TGATCA 1 cut(s) 1981
FblI GTMKAC 2 cut(s) 2831, 3619
FokI GGATG 9 cut(s) 404, 548, 1541, 1830, 2553, 3181, 3682, 3738, 3854
FriOI GRGCYC 2 cut(s) 887, 2001
GlaI GCGC 2 cut(s) 2222, 3361
HaeII RGCGCY 1 cut(s) 2224
HapII CCGG 4 cut(s) 8, 25, 32, 257
HgaI GACGC 1 cut(s) 75
HhaI GCGC 2 cut(s) 2223, 3362
Hin6I GCGC 2 cut(s) 2221, 3360
HinP1I GCGC 2 cut(s) 2221, 3360
HincII GTYRAC 1 cut(s) 3620
HindII GTYRAC 1 cut(s) 3620
HindIII AAGCTT 4 cut(s) 2457, 2616, 3375, 3978
HinfI GANTC 9 cut(s) 743, 782, 2069, 2747, 2812, 3191, 3563, 3657, 3994
HpaII CCGG 4 cut(s) 8, 25, 32, 257
HphI GGTGA 9 cut(s) 28, 214, 638, 1396, 1949, 2039, 2057, 2124, 2717
Hpy166II GTNNAC 5 cut(s) 2755, 2832, 3224, 3620, 3835
Hpy8I GTNNAC 5 cut(s) 2755, 2832, 3224, 3620, 3835
Hpy99I CGWCG 4 cut(s) 175, 2020, 3621, 3624
HpyCH4III ACNGT 9 cut(s) 1208, 1918, 1978, 2579, 2630, 2759, 3301, 3731, 3912
HpyCH4IV ACGT 5 cut(s) 2018, 2174, 2230, 3220, 3622
HpySE526I ACGT 5 cut(s) 2018, 2174, 2230, 3220, 3622
HspAI GCGC 2 cut(s) 2221, 3360
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 1981
LguI GCTCTTC 1 cut(s) 3454
LmnI GCTCC 4 cut(s) 70, 85, 895, 3289
MaeII ACGT 5 cut(s) 2018, 2174, 2230, 3220, 3622
MaeIII GTNAC 4 cut(s) 34, 1316, 3211, 3906
MbiI CCGCTC 1 cut(s) 252
MflI RGATCY 6 cut(s) 1927, 2303, 2650, 3155, 3323, 3581
MhlI GDGCHC 2 cut(s) 887, 2001
MlsI TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
MluNI TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
MlyI GAGTC 2 cut(s) 3572, 4003
MmeI TCCRAC 4 cut(s) 114, 520, 1516, 4064
Mox20I TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
Mph1103I ATGCAT 1 cut(s) 1136
MreI CGCCGGCG 1 cut(s) 7
MroNI GCCGGC 1 cut(s) 7
MroXI GAANNNNTTC 4 cut(s) 664, 1917, 2512, 3979
MscI TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
MslI CAYNNNNRTG 2 cut(s) 3240, 3840
Msp20I TGGCCA 4 cut(s) 1287, 1759, 3557, 3768
MspCI CTTAAG 1 cut(s) 2549
MspI CCGG 4 cut(s) 8, 25, 32, 257
MspR9I CCNGG 2 cut(s) 258, 3999
Mva1269I GAATGC 5 cut(s) 220, 688, 1136, 3247, 3956
MvaI CCWGG 1 cut(s) 3999
MvnI CGCG 3 cut(s) 175, 190, 3360
NaeI GCCGGC 1 cut(s) 9
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 2 cut(s) 1754, 1830
NdeI CATATG 2 cut(s) 3877, 4093
NgoMIV GCCGGC 1 cut(s) 7
NlaIV GGNNCC 3 cut(s) 1330, 1792, 2152
NmeAIII GCCGAG 2 cut(s) 219, 271
NmuCI GTSAC 3 cut(s) 34, 3211, 3906
NruI TCGCGA 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 1136
NspI RCATGY 4 cut(s) 97, 1464, 2338, 2399
PaeI GCATGC 1 cut(s) 2338
PaeR7I CTCGAG 1 cut(s) 3920
PagI TCATGA 2 cut(s) 1273, 2419
PceI AGGCCT 1 cut(s) 2909
PciI ACATGT 1 cut(s) 1460
PciSI GCTCTTC 1 cut(s) 3454
PcsI WCGNNNNNNNCGW 2 cut(s) 2709, 3613
PctI GAATGC 5 cut(s) 220, 688, 1136, 3247, 3956
PdiI GCCGGC 1 cut(s) 9
PdmI GAANNNNTTC 4 cut(s) 664, 1917, 2512, 3979
PfeI GAWTC 7 cut(s) 743, 782, 2069, 2747, 2812, 3191, 3657
PflMI CCANNNNNTGG 1 cut(s) 3379
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 2 cut(s) 3571, 4002
PpsI GAGTC 2 cut(s) 3571, 4002
Ppu21I YACGTR 1 cut(s) 3221
PpuMI RGGWCCY 1 cut(s) 1689
PscI ACATGT 1 cut(s) 1460
PshAI GACNNNNGTC 2 cut(s) 1209, 3411
PsiI TTATAA 1 cut(s) 2463
Psp5II RGGWCCY 1 cut(s) 1689
Psp6I CCWGG 1 cut(s) 3997
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 3997
PspN4I GGNNCC 3 cut(s) 1330, 1792, 2152
PspPI GGNCC 6 cut(s) 158, 228, 254, 1689, 2770, 2857
PspPPI RGGWCCY 1 cut(s) 1689
PstI CTGCAG 4 cut(s) 1372, 3367, 3413, 4007
PstNI CAGNNNCTG 3 cut(s) 698, 1523, 1922
PsuI RGATCY 6 cut(s) 1927, 2303, 2650, 3155, 3323, 3581
PvuI CGATCG 1 cut(s) 179
RruI TCGCGA 1 cut(s) 175
RsaI GTAC 4 cut(s) 875, 2347, 2729, 3885
RsaNI GTAC 4 cut(s) 874, 2346, 2728, 3884
RseI CAYNNNNRTG 2 cut(s) 3240, 3840
Rsr2I CGGWCCG 1 cut(s) 158
RsrII CGGWCCG 1 cut(s) 158
SalI GTCGAC 1 cut(s) 3618
SapI GCTCTTC 1 cut(s) 3454
Sau96I GGNCC 6 cut(s) 158, 228, 254, 1689, 2770, 2857
ScaI AGTACT 2 cut(s) 2729, 3885
SchI GAGTC 2 cut(s) 3572, 4003
ScrFI CCNGG 2 cut(s) 258, 3999
SduI GDGCHC 2 cut(s) 887, 2001
Sfr274I CTCGAG 1 cut(s) 3920
SgrAI CRCCGGYG 1 cut(s) 7
SgrDI CGTCGACG 1 cut(s) 3618
SinI GGWCC 5 cut(s) 158, 228, 1689, 2770, 2857
SlaI CTCGAG 1 cut(s) 3920
SmiI ATTTAAAT 1 cut(s) 927
SmiMI CAYNNNNRTG 2 cut(s) 3240, 3840
SmlI CTYRAG 4 cut(s) 2549, 3113, 3134, 3920
SmoI CTYRAG 4 cut(s) 2549, 3113, 3134, 3920
SphI GCATGC 1 cut(s) 2338
SseBI AGGCCT 1 cut(s) 2909
StuI AGGCCT 1 cut(s) 2909
StyD4I CCNGG 2 cut(s) 256, 3997
StyI CCWWGG 5 cut(s) 1497, 1754, 1830, 2599, 3663
SwaI ATTTAAAT 1 cut(s) 927
TaaI ACNGT 9 cut(s) 1208, 1918, 1978, 2579, 2630, 2759, 3301, 3731, 3912
TaiI ACGT 5 cut(s) 2021, 2177, 2233, 3223, 3625
TaqI TCGA 9 cut(s) 224, 320, 464, 2015, 2712, 3619, 3921, 4063, 4111
TaqII GACCGA 3 cut(s) 146, 174, 2787
TatI WGTACW 3 cut(s) 873, 2727, 3883
TauI GCSGC 4 cut(s) 14, 49, 193, 3503
TfiI GAWTC 7 cut(s) 743, 782, 2069, 2747, 2812, 3191, 3657
TscAI CASTG 2 cut(s) 2890, 3291
TseFI GTSAC 3 cut(s) 34, 3211, 3906
Tsp45I GTSAC 3 cut(s) 34, 3211, 3906
TspGWI ACGGA 5 cut(s) 258, 2363, 3187, 3321, 4019
TspRI CASTG 2 cut(s) 2890, 3291
Van91I CCANNNNNTGG 1 cut(s) 3379
Vha464I CTTAAG 1 cut(s) 2549
VpaK11BI GGWCC 5 cut(s) 158, 228, 1689, 2770, 2857
XbaI TCTAGA 2 cut(s) 3455, 3532
XceI RCATGY 4 cut(s) 97, 1464, 2338, 2399
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XhoI CTCGAG 1 cut(s) 3920
XmiI GTMKAC 2 cut(s) 2831, 3619
XmnI GAANNNNTTC 4 cut(s) 664, 1917, 2512, 3979
ZrmI AGTACT 2 cut(s) 2729, 3885
Zsp2I ATGCAT 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.