MD14G1078300.v1.1

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003093
Physical Location & Seq
Reverse (-)
8937870 .. 8941826
3957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1078300.v1.1.491

Sequence Viewer

Length: 669 bp
ATGAAAGCAGAGGCTCTGTGTTTGTTGCAGAGGTATGAAGAGGCATTTCGTCTGTGTGAACAGAGCAATGAAGCATTTAGGTCAGGAAGCTATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTAGCAATGTTGGATCTCGCCCATTTTCAGCCATCTGTTTGTGCAACCGCGGTGCAGCACACCAAGCATTGGGTCAAATTACTGATGCCATTGCAGACGGCAGTCTTGCCATAGCTCTTGATGGCAATTATGTAAAGGCAGTTTCTAGAAGAGCCACCTTACATGAGATGATACAAGACCATGGAGAATCGGCTAGTGATCTCCAAAGACTTGTATCCATTCTGGAAAACCAATCTAATGACATGGCCAAAGAGTCTGGCTCACTAGGTAGATCCAGTGGCAGTGTAAAAGAATTACGTGATGGTCATCGACGTATGCATTTGATGGAAGAAGCTAAAAAAGGAATCTCCTTGGATTTTTATCTCATTTTGGGAATTAAGCCATCCGATGAGAGTTCTGATATTAAGAAGGCTTACCGTAAGGCAGCGCTGAAGCATCATCCTGACAAGAGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTCCAGAGGTTCACAAGGATGCTGACAGGCTCTTTAAAATGATTGGACAGGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

223

Amino Acids

24.49

Weight (kDa)

6.31

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 161 - 196 4.1e-11 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 196
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 175, 177
AclWI GGATC 2 cut(s) 148, 393
AcoI YGGCCR 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 578
AfeI AGCGCT 1 cut(s) 555
AfiI CCNNNNNNNGG 1 cut(s) 196
AluBI AGCT 3 cut(s) 90, 242, 461
AluI AGCT 3 cut(s) 90, 242, 461
AlwI GGATC 2 cut(s) 148, 393
Aor51HI AGCGCT 1 cut(s) 555
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 182, 551
AspLEI GCGC 1 cut(s) 556
BalI TGGCCA 1 cut(s) 374
BbvI GCAGC 2 cut(s) 194, 563
BccI CCATC 5 cut(s) 167, 242, 422, 445, 517
BceAI ACGGC 1 cut(s) 241
BciVI GTATCC 1 cut(s) 352
BfaI CTAG 4 cut(s) 129, 273, 321, 392
BfoI RGCGCY 1 cut(s) 557
BfuI GTATCC 1 cut(s) 352
BisI GCNGC 2 cut(s) 183, 552
BlsI GCNGC 2 cut(s) 184, 553
BmsI GCATC 3 cut(s) 202, 571, 619
BoxI GACNNNNGTC 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpmI CTGGAG 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 425
BsaBI GATNNNNATC 2 cut(s) 432, 486
BsaJI CCNNGG 3 cut(s) 175, 307, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 402
Bse3DI GCAATG 3 cut(s) 73, 139, 216
Bse8I GATNNNNATC 2 cut(s) 432, 486
BseDI CCNNGG 3 cut(s) 175, 307, 477
BseGI GGATG 3 cut(s) 509, 565, 634
BseJI GATNNNNATC 2 cut(s) 432, 486
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 3 cut(s) 73, 139, 216
BseNI ACTGG 1 cut(s) 402
BseXI GCAGC 2 cut(s) 194, 563
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 196
BsnI GGCC 1 cut(s) 374
Bsp143I GATC 3 cut(s) 140, 325, 398
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 2 cut(s) 175, 177
BspANI GGCC 1 cut(s) 374
BspFNI CGCG 1 cut(s) 177
BspPI GGATC 2 cut(s) 148, 393
BspQI GCTCTTC 1 cut(s) 271
BsrDI GCAATG 3 cut(s) 73, 139, 216
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 3 cut(s) 175, 307, 477
BssMI GATC 3 cut(s) 140, 325, 398
BssT1I CCWWGG 2 cut(s) 307, 477
Bst4CI ACNGT 2 cut(s) 118, 545
Bst6I CTCTTC 2 cut(s) 33, 271
BstBAI YACGTR 1 cut(s) 425
BstDSI CCRYGG 2 cut(s) 175, 307
BstF5I GGATG 3 cut(s) 509, 565, 634
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 557
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 3 cut(s) 143, 328, 401
BstMBI GATC 3 cut(s) 140, 325, 398
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstPAI GACNNNNGTC 1 cut(s) 228
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 2 cut(s) 194, 563
BstX2I RGATCY 2 cut(s) 140, 398
BstYI RGATCY 2 cut(s) 140, 398
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 2 cut(s) 175, 307
BtsCI GGATG 3 cut(s) 509, 565, 634
BtsI GCAGTG 2 cut(s) 108, 415
BtsIMutI CAGTG 3 cut(s) 108, 409, 415
CfoI GCGC 1 cut(s) 556
Cfr42I CCGCGG 1 cut(s) 178
CviAII CATG 3 cut(s) 290, 308, 370
DpnI GATC 3 cut(s) 142, 327, 400
DpnII GATC 3 cut(s) 140, 325, 398
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 33, 271
EarI CTCTTC 2 cut(s) 33, 271
Eco130I CCWWGG 2 cut(s) 307, 477
Eco47III AGCGCT 1 cut(s) 555
Eco57I CTGAAG 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 307, 477
EcoT22I ATGCAT 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 307, 477
FaeI CATG 3 cut(s) 293, 311, 373
FaiI YATR 9 cut(s) 36, 93, 114, 239, 258, 291, 309, 371, 443
FatI CATG 3 cut(s) 289, 307, 369
Fnu4HI GCNGC 2 cut(s) 183, 552
FokI GGATG 3 cut(s) 496, 552, 641
Fsp4HI GCNGC 2 cut(s) 183, 552
FspBI CTAG 4 cut(s) 129, 273, 321, 392
GlaI GCGC 1 cut(s) 555
GluI GCNGC 2 cut(s) 183, 552
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 557
HaeIII GGCC 1 cut(s) 374
HhaI GCGC 1 cut(s) 556
Hin1II CATG 3 cut(s) 293, 311, 373
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HinfI GANTC 3 cut(s) 314, 380, 471
Hpy166II GTNNAC 2 cut(s) 59, 622
Hpy188I TCNGA 2 cut(s) 514, 526
Hpy188III TCNNGA 7 cut(s) 84, 245, 273, 350, 569, 598, 614
Hpy8I GTNNAC 2 cut(s) 59, 622
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 2 cut(s) 529, 581
HpyCH4III ACNGT 2 cut(s) 118, 545
HpyCH4IV ACGT 2 cut(s) 424, 439
HpyCH4V TGCA 5 cut(s) 28, 171, 182, 221, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpySE526I ACGT 2 cut(s) 424, 439
Hsp92II CATG 3 cut(s) 293, 311, 373
HspAI GCGC 1 cut(s) 554
KspI CCGCGG 1 cut(s) 178
Kzo9I GATC 3 cut(s) 140, 325, 398
LguI GCTCTTC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 9 cut(s) 69, 335, 369, 415, 582, 583, 622, 627, 644
Lsp1109I GCAGC 2 cut(s) 194, 563
LweI GCATC 3 cut(s) 202, 571, 619
MaeI CTAG 4 cut(s) 129, 273, 321, 392
MaeII ACGT 2 cut(s) 424, 439
MalI GATC 3 cut(s) 142, 327, 400
MboI GATC 3 cut(s) 140, 325, 398
MboII GAAGA 3 cut(s) 50, 288, 467
MflI RGATCY 2 cut(s) 140, 398
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 4 cut(s) 204, 253, 419, 501
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 4 cut(s) 4, 24, 34, 610
Mox20I TGGCCA 1 cut(s) 374
Mph1103I ATGCAT 1 cut(s) 447
MscI TGGCCA 1 cut(s) 374
MseI TTAA 4 cut(s) 504, 531, 645, 667
MslI CAYNNNNRTG 1 cut(s) 627
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 177
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 191
NcoI CCATGG 1 cut(s) 307
NdeII GATC 3 cut(s) 140, 325, 398
NlaIII CATG 3 cut(s) 293, 311, 373
NsiI ATGCAT 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 314, 471
PflMI CCANNNNNTGG 1 cut(s) 196
PkrI GCNGC 2 cut(s) 184, 553
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 1 cut(s) 425
PshAI GACNNNNGTC 1 cut(s) 228
PsuI RGATCY 2 cut(s) 140, 398
RseI CAYNNNNRTG 1 cut(s) 627
SacII CCGCGG 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 504, 531, 645, 667
SatI GCNGC 2 cut(s) 183, 552
Sau3AI GATC 3 cut(s) 140, 325, 398
SchI GAGTC 1 cut(s) 389
SfaNI GCATC 3 cut(s) 202, 571, 619
Sfr303I CCGCGG 1 cut(s) 178
SgrBI CCGCGG 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 627
Sse9I AATT 4 cut(s) 204, 253, 419, 501
SsiI CCGC 2 cut(s) 175, 177
SspMI CTAG 4 cut(s) 129, 273, 321, 392
StyI CCWWGG 2 cut(s) 307, 477
TaaI ACNGT 2 cut(s) 118, 545
TaiI ACGT 2 cut(s) 427, 442
TaqI TCGA 1 cut(s) 436
TasI AATT 4 cut(s) 204, 253, 419, 501
TfiI GAWTC 2 cut(s) 314, 471
Tru1I TTAA 4 cut(s) 504, 531, 645, 667
Tru9I TTAA 4 cut(s) 504, 531, 645, 667
TscAI CASTG 3 cut(s) 108, 409, 415
TseI GCWGC 2 cut(s) 182, 551
TspDTI ATGAA 4 cut(s) 17, 51, 84, 600
TspRI CASTG 3 cut(s) 108, 409, 415
Van91I CCANNNNNTGG 1 cut(s) 196
XbaI TCTAGA 1 cut(s) 272
XspI CTAG 4 cut(s) 129, 273, 321, 392
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.