Rw3G023280

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003093
Physical Location & Seq
Reverse (-)
29144208 .. 29152714
8507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023280.1

Sequence Viewer

Length: 4029 bp
ATGAGGCCGGCGGCGGCGGACGTCCGGTCCCCGATTACCTCTAAACCCTCCGGCAATCCTCAAAACCCTAAACCTTCATCGGAGGAGACGCCCTCCTCTAACCCCTTCAATTTCAATGCTGCTGGTTCCGACGGTCCGTCGTGTCCAAATGTGCCGTCACGATCAGCTCGGCCGCGGCCGAGGTTGGCGAAGCACAGGAGGAAGCAGCATGCGAATGCGGGGTCGAGAACTGGGCCGGCTGAGACGGCTGGGCCGGAGGTCAACCCGTTTTGCTCGGTAAGTGGTGGTGCTGCTACTAGTGTTTTTAATCAGTTCAGTTTTCCGAGTGGTGGTGTGGGTTTTGTGTTTGCCGCTACATTGAGTGGTGTTGATGAGCGGGCTCAGGTGAACGGTGGGGATGGTAGAGAGTTCGGTGAGGTTGAGAGTGTGAGTGCTAAGCAGGATGATCCTCCGGGGCCGAGTTCGTGTGGGGAGAGGAGAGAATGTGACACTGATCAAAGTGGAAAAGGGATATTCGAGTGTGATCAGAATCATTCAGCTCCGAATTTGAGTGCCGAAAATGGAGAATCGGCAGAATGTGTTGAGAGACTCGGTGATTTTGGGAGTGGTTCAGAGGTAGGACGGAGTGAATATTGTGAAAATGAGGGGAAGTCAGGTAATGACTCTAGTGGTATTGGTTTTGTGTTTGGTTCCGGTTGGTTTAATTCAAGTTCAGATTTGAATTCAGGAAAGAATGACTCTAGTGGAAATGTGGAAAAATTGGTTGATGAAGTTGGCAGGAAGATGAGAGTAGAGAGTGACACCGAGTTTGAGAAAGTGAAAGTTAATTCTTTCGAATTTCATACTGAAGAGCGTTGGAGCTCAGACAAGGTTCATGATGAGGGCATTTTTGTATTTGGAAGCAGCGGTAAGAAAGGTTCCAGTTTGACGGATTCCAAGGTAGCTAATTGTCAAGACGAGGTGAAATCAAGTGATGAAGGAGATTGCAAGTCCAATTCAAACAGTCGGAATGATAATCTGAGTTCTGATTTCAGTAGCAAGTGTAATCTTGTATCTGAAACTAGTGAGGTAACTTGTGGAGATAATCATAAAAGTACTGACCATGTTGTATTTGGAAGCGAAAGTAACACGGCTGGTACCACCAGTGGGATTTCTAGTTCCACTGAGTTTACATTTCAGGCAGGACTTGGTGATAGTGTTGATGCAGGCCAGTTTCCTGAGTGTCAAATTAATAAACCCAATGCAGCTGCTGGCCCATGTTCATTCTCATCAATTGGTCTTGGCATCCAACTAAATGGTTGTGTTTCTGAAGCAGCTTCTGCGGGTGGAGTAAAAGGTAAAGATGAACGTAGCTTTACTACCTCTCCAGATGGATTTGGAGTGTGCTTTGAGGACTTCAACACTCCATTGCGGTTTCCTGCTTGCCTCAAAGAAAATCTATTTCCTGAAATAAATAAATCAAACATCAGTATGAAGGGTAGATCGGTTAGGGACAAGGGATCAAGGAAAACAAGGGGAAGGTTGAAGAAACCTTCTCTGAGTAAGCAATGGCCATTGCAAGATAATGTTCCCAAGGAAAGTAGTTCCCAAGAAAACCCAGTCTCACCTGGATGTGGCTCACCCATGGATTTCTCTCCATATGAGGAAACTACAGTTGCTGATCCACATTCGAGGGAATCTTCTACAATGTCTAATGACACCTTCCACCTAGTCGGTAATACTGCACCTTTTGGCTCCGATGCAACAGTTCCCATTGGACGTAGTGGTGATGAGGGAGAATTTGCCGCTAACGTTGATCAGATATATGGTAAGGCCACTGACGAGAATTCTAGGTATCACGGTGAAAGAATATTTGTTGACAATTGTTCCTCCAAAGGGTCTGGTTCTGGAGCTGAAACTCCCTGCTTTAGCTCCAATGGTATCAGTCATGCTGGTAAGGATTCAGAAGACGTTAGAGCTGATAATGGTGTGAATGTAGAAAGTCAAGAACAGGATTGTGGAACACCGTGTTTTGCTTCTGGTTTGAAAAATATGGAGAGGAAAACATTTACCTTTGTTGCATCATCTTCTGCAGAAGGAAGTCCAATAGTGGCAAGGCGTCTTCCGCACAGAGTGAAAAATAAGAAGAAAGTTGGTTCTAAAACGTTTGTTATAACTCCAATTCCAAATGTTCAGTTTGGAGGTTCTGACCAATTTACACCCCACTCTAGCACACCATTGTCTTTTGATGCGGTAGGAAAATCTGAGCCCGATGAACAAGTAACGAAAGTGCATATCCCATCTACTGAGGCTACTCATGAGACTTGTGAGAAGTGGCGTATTAGGGGCAACAAAGCTTATAGGAACAAAGACTTTTCAAAAGCTGAAGACTTTTACACACAGGGAATAGTTTCTGTACCTTCAAGTGAAAGATCAGGAAGCTGCCTTAGGCCTCTTGTACTATGCTATAGCAATCGTGCAGCAACAAGGATGTATATGGGAAGGATAAGGGAAGCTTTAGGGGACTGCATGATGGCCACTGCACTAGACCCCAACTTTCTCAAAGTTCAAATGAGAGCTGCAAATTTCCATCTTCTTCTCGGCGAAGTTGAAAATGCCCAACGGTACTTTAATAATTGCTTGGAATCCAGAACTGTTGTGTGTTTGGATCGGAGAATTGTAATTGATTCTGCTGATGGCATACAAAAGGCTCAGAAAGTGGCTGAGGATACGAATCGCTCTGCCAAGCTTCTGGAACAGAGAACTGCAGATGCAGCTCTCAGTGCCTTGGAAATTGTTTCTGAGGCCTTGTCTATTAGTGAATACTCGGAAAAGTTACTTGAAATGAAGGCAGAGGCTTTGTGTTTGTTGAGGAGATATGAAGAGGCAATTCAACTGTGTGAACAGAGTATGTATTTTGCTGAGAAGAACTTTTCTTCGCTGAACAGTGTAGTAAATATGGATGCTGCTGGATGTGAAGATCATCCATATGCTAGGTTGTGGAGGTGGTTCTTCATATCCAAATGTTACTTTCATTTGGGAAGGTTTCAGGCAGCACTTGATTTACTTGAGAAGCTAGAGCAAGTGGGATCTATCAAAGACAGGTATGCGAGTAAGAATCTGGAATCATCAATTTCATTAGCTGCCACCATACGTGAACTGTTAAGCCACAAGGATGCAGGGAATGAAGCATTTAGATCAGGAAGATATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTCGCAATATTGGATCTCGGCCATTTTCTGCAATCTGTTTGTGCAACCGTGCTGCAGCACACCAAGCTTTGGGTCAAATTACTGATGCCATTGCTGACTGCAGTCTTGCAATAGCTCTTGATGGACATTATGTTAAGGCAGTTTCTAGAAGAGCTACCTTACATGAGATGATACGGGACTATGGACAGGCTGCTAGCGATCTCCAAAGACTTATTTCTGTACTGGAAAACCAATCTGGTGACAAGGCAAAAGAACCTACCTCACAAGGTAGCTCCAATGGCCGTACACAAGAATTACGGAACTCTTATCGACGTATGCCTTTAATGGAACAAGAAGCTAAGAAAGGAATCCCCTTGAATTTTTACCTCATTCTGGGAATTAAATCATCTGACACAAGTTCTGACATTAAGAAGGCATACCGTAAGGCTGCTCTCAAGCATCATCCCGACAAGGCTGGTCAGTTCTTGGCAAGAAGTGAGAGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTCTCGAGGTTCACAAGGATGCTGACAGACTCTTTAAAATGATCGGAGAGGCATATGCAGTACTTTCAGATCCCAACAAGCGCTCAGAATATGATCTTGAGGAAGACATAAGAAAATCAGCAAAGGAAAGCAAGGAACGTGGCGTCTACAGAAAGTCTTCAGATTTTCAGAGTCCTAGACGCAGCAGCTATAGAAAACCAGATTTTTATAGCTCTCCATTCGAGAGAAGCTCATATTGTCGGAACAGTAGGGAGAGTTGGAAGACATATGGAGATTCGTATTCACGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

1342

Amino Acids

146.44

Weight (kDa)

5.97

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1194 - 1226 1.8e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2153
AatII GACGTC 1 cut(s) 24
Acc65I GGTACC 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 1136
AccB7I CCANNNNNTGG 1 cut(s) 3292
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 3882
AccII CGCG 1 cut(s) 175
AclI AACGTT 2 cut(s) 1791, 2144
AclWI GGATC 7 cut(s) 440, 1507, 1655, 2655, 3076, 3244, 3800
AcoI YGGCCR 6 cut(s) 170, 176, 1550, 2514, 3242, 3502
AcsI RAATTY 7 cut(s) 544, 721, 836, 1778, 1825, 2563, 3580
AcuI CTGAAG 4 cut(s) 867, 1329, 2385, 3879
AcyI GRCGYC 4 cut(s) 21, 89, 2098, 3879
AdeI CACNNNGTG 1 cut(s) 2113
AfaI GTAC 8 cut(s) 1096, 1138, 2397, 2439, 2606, 3444, 3508, 3798
AfeI AGCGCT 1 cut(s) 3818
AfiI CCNNNNNNNGG 6 cut(s) 329, 1146, 1613, 1875, 3292, 3595
AhdI GACNNNNNGTC 2 cut(s) 25, 136
AhlI ACTAGT 2 cut(s) 296, 1061
AjnI CCWGG 1 cut(s) 1606
AloI GAACNNNNNNTCC 2 cut(s) 1141, 1173
Alw21I GWGCWC 1 cut(s) 863
Alw26I GTCTC 5 cut(s) 80, 236, 580, 1606, 2294
AlwI GGATC 7 cut(s) 440, 1507, 1655, 2655, 3076, 3244, 3800
AlwNI CAGNNNCTG 3 cut(s) 1250, 1319, 1658
Ama87I CYCGRG 1 cut(s) 3740
Aor51HI AGCGCT 1 cut(s) 3818
ApoI RAATTY 7 cut(s) 544, 721, 836, 1778, 1825, 2563, 3580
ArsI GACNNNNNNTTYG 6 cut(s) 140, 172, 206, 238, 791, 823
AseI ATTAAT 1 cut(s) 1230
Asp700I GAANNNNTTC 2 cut(s) 2389, 3892
Asp718I GGTACC 1 cut(s) 1136
AspLEI GCGC 1 cut(s) 3819
AspS9I GGNCC 6 cut(s) 27, 134, 233, 251, 455, 1253
AsuC2I CCSGG 1 cut(s) 453
AsuII TTCGAA 1 cut(s) 834
AsuNHI GCTAGC 1 cut(s) 3416
AvaI CYCGRG 1 cut(s) 3740
AvaII GGWCC 2 cut(s) 27, 134
AxyI CCTNAGG 1 cut(s) 2426
BalI TGGCCA 2 cut(s) 1552, 2516
BanI GGYRCC 1 cut(s) 1136
BanII GRGCYC 3 cut(s) 382, 863, 2250
BbsI GAAGAC 6 cut(s) 1953, 2093, 2373, 3846, 3885, 4004
Bbv12I GWGCWC 1 cut(s) 863
BbvCI CCTCAGC 1 cut(s) 2703
BccI CCATC 8 cut(s) 392, 1364, 2287, 2506, 2577, 2669, 3338, 4017
BceAI ACGGC 4 cut(s) 139, 261, 1146, 3489
BciT130I CCWGG 1 cut(s) 1608
BciVI GTATCC 1 cut(s) 2701
BclI TGATCA 3 cut(s) 493, 523, 1795
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 5 cut(s) 80, 236, 580, 1606, 2294
BcuI ACTAGT 2 cut(s) 296, 1061
BfmI CTRYAG 8 cut(s) 1650, 2070, 2446, 2745, 3276, 3322, 3883, 3925
BfoI RGCGCY 1 cut(s) 3820
BfuI GTATCC 1 cut(s) 2701
BlpI GCTNAGC 1 cut(s) 435
BmcAI AGTACT 2 cut(s) 1096, 3798
Bme1390I CCNGG 2 cut(s) 453, 1608
Bme18I GGWCC 2 cut(s) 27, 134
BmeRI GACNNNNNGTC 2 cut(s) 25, 136
BmeT110I CYCGRG 1 cut(s) 3740
BmgT120I GGNCC 6 cut(s) 27, 134, 233, 251, 455, 1253
BmiI GGNNCC 7 cut(s) 29, 127, 456, 691, 919, 1138, 1735
BmrFI CCNGG 2 cut(s) 453, 1608
BmrI ACTGGG 2 cut(s) 240, 1592
BmtI GCTAGC 1 cut(s) 3420
BmuI ACTGGG 2 cut(s) 240, 1592
BoxI GACNNNNGTC 1 cut(s) 3324
BpiI GAAGAC 6 cut(s) 1953, 2093, 2373, 3846, 3885, 4004
BplI GAGNNNNNCTC 4 cut(s) 77, 109, 3950, 3982
BpmI CTGGAG 2 cut(s) 1350, 1908
Bpu10I CCTNAGC 2 cut(s) 381, 2703
Bpu1102I GCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 3 cut(s) 3068, 3641, 3854
BpuMI CCSGG 1 cut(s) 453
BsaAI YACGTR 1 cut(s) 3134
BsaBI GATNNNNATC 2 cut(s) 528, 2712
BsaHI GRCGYC 4 cut(s) 21, 89, 2098, 3879
BsaJI CCNNGG 7 cut(s) 173, 179, 452, 936, 1572, 1623, 2766
BsaWI WCCGGW 2 cut(s) 24, 692
BsaXI ACNNNNNCTCC 4 cut(s) 469, 499, 1348, 1378
Bsc4I CCNNNNNNNGG 6 cut(s) 329, 1146, 1613, 1875, 3292, 3595
Bse118I RCCGGY 2 cut(s) 7, 235
Bse1I ACTGG 6 cut(s) 235, 921, 1143, 1210, 1598, 3450
Bse21I CCTNAGG 1 cut(s) 2426
Bse3DI GCAATG 4 cut(s) 1406, 1553, 1553, 3312
Bse8I GATNNNNATC 2 cut(s) 528, 2712
BseBI CCWGG 1 cut(s) 1608
BseDI CCNNGG 7 cut(s) 173, 179, 452, 936, 1572, 1623, 2766
BseJI GATNNNNATC 2 cut(s) 528, 2712
BseLI CCNNNNNNNGG 6 cut(s) 329, 1146, 1613, 1875, 3292, 3595
BseMI GCAATG 4 cut(s) 1406, 1553, 1553, 3312
BseNI ACTGG 6 cut(s) 235, 921, 1143, 1210, 1598, 3450
BseRI GAGGAG 4 cut(s) 85, 98, 490, 2866
BseX3I CGGCCG 2 cut(s) 170, 176
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 3 cut(s) 1707, 2478, 2505
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 2 cut(s) 173, 179
BshNI GGYRCC 1 cut(s) 1136
BsiEI CGRYCG 2 cut(s) 173, 179
BsiHKAI GWGCWC 1 cut(s) 863
BsiHKCI CYCGRG 1 cut(s) 3740
BsiSI CCGG 7 cut(s) 8, 25, 51, 236, 254, 452, 693
BslFI GGGAC 4 cut(s) 13, 1505, 2516, 3413
BslI CCNNNNNNNGG 6 cut(s) 329, 1146, 1613, 1875, 3292, 3595
BsmAI GTCTC 5 cut(s) 80, 236, 580, 1606, 2294
BsmBI CGTCTC 2 cut(s) 80, 236
BsmFI GGGAC 4 cut(s) 13, 1505, 2516, 3413
BsmI GAATGC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 3740
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 3 cut(s) 382, 863, 2250
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 1 cut(s) 1623
BspFNI CGCG 1 cut(s) 175
BspHI TCATGA 2 cut(s) 874, 2296
BspLI GGNNCC 7 cut(s) 29, 127, 456, 691, 919, 1138, 1735
BspMAI CTGCAG 4 cut(s) 2074, 2749, 3280, 3326
BspOI GCTAGC 1 cut(s) 3420
BspPI GGATC 7 cut(s) 440, 1507, 1655, 2655, 3076, 3244, 3800
BspQI GCTCTTC 2 cut(s) 843, 3367
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 1136
BsrBI CCGCTC 1 cut(s) 376
BsrDI GCAATG 4 cut(s) 1406, 1553, 1553, 3312
BsrFI RCCGGY 2 cut(s) 7, 235
BsrI ACTGG 6 cut(s) 235, 921, 1143, 1210, 1598, 3450
BssAI RCCGGY 2 cut(s) 7, 235
BssECI CCNNGG 7 cut(s) 173, 179, 452, 936, 1572, 1623, 2766
BssNI GRCGYC 4 cut(s) 21, 89, 2098, 3879
BssT1I CCWWGG 4 cut(s) 936, 1572, 1623, 2766
Bst2UI CCWGG 1 cut(s) 1608
Bst6I CTCTTC 3 cut(s) 843, 2856, 3367
BstACI GRCGYC 4 cut(s) 21, 89, 2098, 3879
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstBAI YACGTR 1 cut(s) 3134
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 8 cut(s) 9, 210, 237, 378, 1207, 1252, 1423, 3418
BstDSI CCRYGG 2 cut(s) 173, 1623
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 3820
BstHHI GCGC 1 cut(s) 3819
BstMAI GTCTC 5 cut(s) 80, 236, 580, 1606, 2294
BstMCI CGRYCG 2 cut(s) 173, 179
BstMWI GCNNNNNNNGC 7 cut(s) 245, 1319, 2104, 2753, 2762, 3287, 3501
BstNI CCWGG 1 cut(s) 1608
BstNSI RCATGY 1 cut(s) 212
BstPAI GACNNNNGTC 1 cut(s) 3324
BstSCI CCNGG 2 cut(s) 451, 1606
BstSFI CTRYAG 8 cut(s) 1650, 2070, 2446, 2745, 3276, 3322, 3883, 3925
BstUI CGCG 1 cut(s) 175
BstV2I GAAGAC 6 cut(s) 1953, 2093, 2373, 3846, 3885, 4004
BstX2I RGATCY 3 cut(s) 3068, 3236, 3805
BstXI CCANNNNNNTGG 2 cut(s) 1295, 2731
BstYI RGATCY 3 cut(s) 3068, 3236, 3805
BstZI CGGCCG 2 cut(s) 170, 176
Bsu36I CCTNAGG 1 cut(s) 2426
BsuI GTATCC 1 cut(s) 2701
BtgI CCRYGG 2 cut(s) 173, 1623
BtsI GCAGTG 2 cut(s) 2517, 3204
BtsIMutI CAGTG 8 cut(s) 489, 1150, 1161, 1815, 2517, 2767, 2932, 3204
Cac8I GCNNGC 8 cut(s) 9, 210, 237, 378, 1207, 1252, 1423, 3418
CaiI CAGNNNCTG 3 cut(s) 1250, 1319, 1658
CciI TCATGA 2 cut(s) 874, 2296
CfoI GCGC 1 cut(s) 3819
Cfr10I RCCGGY 2 cut(s) 7, 235
Cfr13I GGNCC 6 cut(s) 27, 134, 233, 251, 455, 1253
Cfr42I CCGCGG 1 cut(s) 176
CpoI CGGWCCG 1 cut(s) 134
CseI GACGC 4 cut(s) 97, 2087, 3868, 3924
Csp6I GTAC 8 cut(s) 1095, 1137, 2396, 2438, 2605, 3443, 3507, 3797
CspCI CAANNNNNGTGG 2 cut(s) 3856, 3891
CspI CGGWCCG 1 cut(s) 134
CviAII CATG 9 cut(s) 209, 875, 1103, 1257, 1624, 1928, 2297, 2509, 3386
CviQI GTAC 8 cut(s) 1095, 1137, 2396, 2438, 2605, 3443, 3507, 3797
DraI TTTAAA 1 cut(s) 3772
DraIII CACNNNGTG 1 cut(s) 2113
DriI GACNNNNNGTC 2 cut(s) 25, 136
EaeI YGGCCR 6 cut(s) 170, 176, 1550, 2514, 3242, 3502
EagI CGGCCG 2 cut(s) 170, 176
Eam1104I CTCTTC 3 cut(s) 843, 2856, 3367
Eam1105I GACNNNNNGTC 2 cut(s) 25, 136
EarI CTCTTC 3 cut(s) 843, 2856, 3367
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 861
EclXI CGGCCG 2 cut(s) 170, 176
Eco130I CCWWGG 4 cut(s) 936, 1572, 1623, 2766
Eco147I AGGCCT 2 cut(s) 2431, 2786
Eco24I GRGCYC 3 cut(s) 382, 863, 2250
Eco47I GGWCC 2 cut(s) 27, 134
Eco47III AGCGCT 1 cut(s) 3818
Eco52I CGGCCG 2 cut(s) 170, 176
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 4 cut(s) 867, 1329, 2385, 3879
Eco81I CCTNAGG 1 cut(s) 2426
Eco88I CYCGRG 1 cut(s) 3740
EcoICRI GAGCTC 1 cut(s) 861
EcoRI GAATTC 2 cut(s) 721, 1825
EcoRII CCWGG 1 cut(s) 1606
EcoT14I CCWWGG 4 cut(s) 936, 1572, 1623, 2766
EcoT38I GRGCYC 3 cut(s) 382, 863, 2250
ErhI CCWWGG 4 cut(s) 936, 1572, 1623, 2766
Esp3I CGTCTC 2 cut(s) 80, 236
FaeI CATG 9 cut(s) 212, 878, 1106, 1260, 1627, 1931, 2300, 2512, 3389
FalI AAGNNNNNCTT 2 cut(s) 2479, 2511
FaqI GGGAC 4 cut(s) 13, 1505, 2516, 3413
FatI CATG 9 cut(s) 208, 874, 1102, 1256, 1623, 1927, 2296, 2508, 3385
FauI CCCGC 3 cut(s) 211, 369, 1315
FauNDI CATATG 4 cut(s) 1639, 2968, 3790, 4003
FbaI TGATCA 3 cut(s) 493, 523, 1795
FblI GTMKAC 1 cut(s) 3882
FriOI GRGCYC 3 cut(s) 382, 863, 2250
GlaI GCGC 1 cut(s) 3818
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 2 cut(s) 1350, 1908
HaeII RGCGCY 1 cut(s) 3820
HapII CCGG 7 cut(s) 8, 25, 51, 236, 254, 452, 693
HgaI GACGC 4 cut(s) 97, 2087, 3868, 3924
HhaI GCGC 1 cut(s) 3819
Hin1I GRCGYC 4 cut(s) 21, 89, 2098, 3879
Hin1II CATG 9 cut(s) 212, 878, 1106, 1260, 1627, 1931, 2300, 2512, 3389
Hin6I GCGC 1 cut(s) 3817
HinP1I GCGC 1 cut(s) 3817
HincII GTYRAC 2 cut(s) 262, 1858
HindII GTYRAC 2 cut(s) 262, 1858
HindIII AAGCTT 4 cut(s) 2334, 2493, 2726, 3288
HpaII CCGG 7 cut(s) 8, 25, 51, 236, 254, 452, 693
Hpy166II GTNNAC 9 cut(s) 262, 388, 1170, 1858, 2882, 3137, 3509, 3748, 3883
Hpy8I GTNNAC 9 cut(s) 262, 388, 1170, 1858, 2882, 3137, 3509, 3748, 3883
Hpy99I CGWCG 3 cut(s) 134, 142, 3537
HpyCH4IV ACGT 9 cut(s) 21, 1348, 1759, 1791, 1950, 2144, 3133, 3535, 3874
HpyF10VI GCNNNNNNNGC 7 cut(s) 245, 1319, 2104, 2753, 2762, 3287, 3501
HpySE526I ACGT 9 cut(s) 21, 1348, 1759, 1791, 1950, 2144, 3133, 3535, 3874
Hsp92I GRCGYC 4 cut(s) 21, 89, 2098, 3879
Hsp92II CATG 9 cut(s) 212, 878, 1106, 1260, 1627, 1931, 2300, 2512, 3389
HspAI GCGC 1 cut(s) 3817
KpnI GGTACC 1 cut(s) 1140
KroI GCCGGC 2 cut(s) 7, 235
KroNI GCCGGC 2 cut(s) 9, 237
Ksp22I TGATCA 3 cut(s) 493, 523, 1795
KspI CCGCGG 1 cut(s) 176
LguI GCTCTTC 2 cut(s) 843, 3367
LmnI GCTCC 7 cut(s) 544, 858, 1739, 1889, 1916, 3202, 3500
MaeII ACGT 9 cut(s) 21, 1348, 1759, 1791, 1950, 2144, 3133, 3535, 3874
MaeIII GTNAC 9 cut(s) 156, 485, 797, 1069, 1124, 2260, 2814, 3005, 3461
MbiI CCGCTC 1 cut(s) 376
MfeI CAATTG 2 cut(s) 1272, 1861
MflI RGATCY 3 cut(s) 3068, 3236, 3805
MhlI GDGCHC 3 cut(s) 382, 863, 2250
MlsI TGGCCA 2 cut(s) 1552, 2516
MluNI TGGCCA 2 cut(s) 1552, 2516
MlyI GAGTC 5 cut(s) 582, 656, 731, 3759, 3916
MmeI TCCRAC 6 cut(s) 153, 836, 986, 1312, 3956, 3974
Mox20I TGGCCA 2 cut(s) 1552, 2516
MroNI GCCGGC 2 cut(s) 7, 235
MroXI GAANNNNTTC 2 cut(s) 2389, 3892
MscI TGGCCA 2 cut(s) 1552, 2516
MslI CAYNNNNRTG 6 cut(s) 213, 1469, 1609, 2967, 3153, 3753
Msp20I TGGCCA 2 cut(s) 1552, 2516
MspA1I CMGCKG 3 cut(s) 175, 906, 1247
MspI CCGG 7 cut(s) 8, 25, 51, 236, 254, 452, 693
MspR9I CCNGG 2 cut(s) 453, 1608
MunI CAATTG 2 cut(s) 1272, 1861
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 1608
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 7 cut(s) 245, 1319, 2104, 2753, 2762, 3287, 3501
NaeI GCCGGC 2 cut(s) 9, 237
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 1623
NdeI CATATG 4 cut(s) 1639, 2968, 3790, 4003
NgoMIV GCCGGC 2 cut(s) 7, 235
NheI GCTAGC 1 cut(s) 3416
NlaIII CATG 9 cut(s) 212, 878, 1106, 1260, 1627, 1931, 2300, 2512, 3389
NlaIV GGNNCC 7 cut(s) 29, 127, 456, 691, 919, 1138, 1735
NmeAIII GCCGAG 5 cut(s) 148, 204, 483, 2559, 3220
NmuCI GTSAC 4 cut(s) 156, 485, 797, 3461
NspI RCATGY 1 cut(s) 212
NspV TTCGAA 1 cut(s) 834
PaeI GCATGC 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 3740
PagI TCATGA 2 cut(s) 874, 2296
PceI AGGCCT 2 cut(s) 2431, 2786
PciSI GCTCTTC 2 cut(s) 843, 3367
PctI GAATGC 1 cut(s) 220
PdiI GCCGGC 2 cut(s) 9, 237
PdmI GAANNNNTTC 2 cut(s) 2389, 3892
PflMI CCANNNNNTGG 1 cut(s) 3292
PleI GAGTC 5 cut(s) 582, 656, 731, 3759, 3915
PpsI GAGTC 5 cut(s) 582, 656, 731, 3759, 3915
Ppu21I YACGTR 1 cut(s) 3134
PshAI GACNNNNGTC 1 cut(s) 3324
PshBI ATTAAT 1 cut(s) 1230
PsiI TTATAA 1 cut(s) 2153
Psp124BI GAGCTC 1 cut(s) 863
Psp1406I AACGTT 2 cut(s) 1791, 2144
Psp6I CCWGG 1 cut(s) 1606
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 1606
PspN4I GGNNCC 7 cut(s) 29, 127, 456, 691, 919, 1138, 1735
PspPI GGNCC 6 cut(s) 27, 134, 233, 251, 455, 1253
PsrI GAACNNNNNNTAC 2 cut(s) 901, 933
PstI CTGCAG 4 cut(s) 2074, 2749, 3280, 3326
PstNI CAGNNNCTG 3 cut(s) 1250, 1319, 1658
PsuI RGATCY 3 cut(s) 3068, 3236, 3805
PvuII CAGCTG 1 cut(s) 1247
RsaI GTAC 8 cut(s) 1096, 1138, 2397, 2439, 2606, 3444, 3508, 3798
RsaNI GTAC 8 cut(s) 1095, 1137, 2396, 2438, 2605, 3443, 3507, 3797
RseI CAYNNNNRTG 6 cut(s) 213, 1469, 1609, 2967, 3153, 3753
Rsr2I CGGWCCG 1 cut(s) 134
RsrII CGGWCCG 1 cut(s) 134
SacI GAGCTC 1 cut(s) 863
SacII CCGCGG 1 cut(s) 176
SapI GCTCTTC 2 cut(s) 843, 3367
Sau96I GGNCC 6 cut(s) 27, 134, 233, 251, 455, 1253
ScaI AGTACT 2 cut(s) 1096, 3798
SchI GAGTC 5 cut(s) 582, 656, 731, 3759, 3916
ScrFI CCNGG 2 cut(s) 453, 1608
SduI GDGCHC 3 cut(s) 382, 863, 2250
SfcI CTRYAG 8 cut(s) 1650, 2070, 2446, 2745, 3276, 3322, 3883, 3925
Sfr274I CTCGAG 1 cut(s) 3740
Sfr303I CCGCGG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 834
SgrBI CCGCGG 1 cut(s) 176
SinI GGWCC 2 cut(s) 27, 134
SlaI CTCGAG 1 cut(s) 3740
SmiMI CAYNNNNRTG 6 cut(s) 213, 1469, 1609, 2967, 3153, 3753
SmlI CTYRAG 4 cut(s) 3047, 3656, 3740, 3833
SmoI CTYRAG 4 cut(s) 3047, 3656, 3740, 3833
SpeI ACTAGT 2 cut(s) 296, 1061
SphI GCATGC 1 cut(s) 212
SseBI AGGCCT 2 cut(s) 2431, 2786
SspI AATATT 3 cut(s) 632, 1851, 3232
SstI GAGCTC 1 cut(s) 863
StuI AGGCCT 2 cut(s) 2431, 2786
StyD4I CCNGG 2 cut(s) 451, 1606
StyI CCWWGG 4 cut(s) 936, 1572, 1623, 2766
TaiI ACGT 9 cut(s) 24, 1351, 1762, 1794, 1953, 2147, 3136, 3538, 3877
TaqI TCGA 7 cut(s) 224, 516, 834, 1670, 3532, 3741, 3957
TatI WGTACW 4 cut(s) 1094, 2437, 3442, 3796
TauI GCSGC 6 cut(s) 14, 17, 175, 178, 353, 1787
TscAI CASTG 8 cut(s) 496, 1150, 1168, 1822, 2524, 2767, 2932, 3204
TseFI GTSAC 4 cut(s) 156, 485, 797, 3461
Tsp45I GTSAC 4 cut(s) 156, 485, 797, 3461
TspGWI ACGGA 4 cut(s) 126, 637, 944, 3535
TspRI CASTG 8 cut(s) 496, 1150, 1168, 1822, 2524, 2767, 2932, 3204
Van91I CCANNNNNTGG 1 cut(s) 3292
VpaK11BI GGWCC 2 cut(s) 27, 134
VspI ATTAAT 1 cut(s) 1230
XapI RAATTY 7 cut(s) 544, 721, 836, 1778, 1825, 2563, 3580
XbaI TCTAGA 1 cut(s) 3368
XceI RCATGY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XhoI CTCGAG 1 cut(s) 3740
XmiI GTMKAC 1 cut(s) 3882
XmnI GAANNNNTTC 2 cut(s) 2389, 3892
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 2 cut(s) 1096, 3798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.