pycom12g07010

dnaJ homolog subfamily C member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003093
Physical Location & Seq
Reverse (-)
7280846 .. 7289751
8906 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g07010.1

Sequence Viewer

Length: 4125 bp
ATGTCGCCGGCGGCAGTTGATGTCCGGCCTCCGGTCATCTCTCCGCCGCCCAAACCCAAACCCTCATCTTCTCCTCAAAATTCTGCTCCTGCGTCCGACATGCCTTCCTCTTCTACTTTCAATCTCGGTGCTTCAGACGGCAATGGGAGTAAGTGTCAGTTCGGTTCGAGTGTGCCGTCGCGATCGACTCGGTCGCGGCCGAGGTTGGTGAAGCTGAGGAAGCAGCATTCGAGGTCCAGAACTGGGTCGGCCGAGAGCGGGCCGGGGGTCAACCCGTTTTGCTCGGTAAGTGATGGTACTAGTGCTTCTAATGGGTTTAATTTTTCGACTGGCGATTGTGGGGGTGTGGGTTTTGTGTTTGGTGCTAAGAGTGGGGTTGAGAATTTGGATAATGGGGATGGAGAGAGTGATGGAATAGTGAGATATGTGGATAATGCGGAAAGAGGAAGTGATGGGGTAGCGGGAACTTCGAGTTATGATGATAATTTGGATAAAGGGGAAGGAGTGAAAACTTTGAATTGTGATGAGAGGGGGCAGATGGATGTCGGAAATGGAAGAGAGTTTGATAAGGTTGAGAGTGATAGTTTTGAGTGCGGTGCAAAGAACGATGATCTGGGATCGCTTTTGTGCGGTGCGAAGAGAGAATTTTATGAAAATGTGAGAGGAAAGATTTCTGAAGATAAGTGGAATGCAAAAACAGATACTGAAACAGAATGTCAGAAGGTTGATAACATGGGTTTTTCTTTTGATTCTAATAGCAGTGATTTGGGTATGCATTTGAATTTGGATTCAAATCCAGAAATGCAAGAGTGTGATGGAAATGTGGAGAAACCGAGCATGGATAATAGGGGGGGAATGAAATTAGAGAGTGAAGTAGAGTACAATGTGGGCTCCGCTCCTTTCGTGTTTTGTTCTAGTTTGATTTCGAATTTAAATTCAAATATGGAAAAGAAAAAACCTAGTGGAAATCTGGACAAGTTGGCTTCTGATGTTGGCAGAAAGCTGAAAGTAGAGAGTGAAACAGACTTAGAGAAGGTGGAAGCTGATCCTTTTGAATTTCATGCTGAGAAGAGTTGCATCTTAAATAAGGATCAAGAGAATGCCTTTTTGGTATTTAGAAGCAGCAGTTATACTGAATGCATGGAACCCAAGTGTCAAGATACAATGAAACTGAGTAGTGAAAATTTAGGAGGTTTCAAGACAAATTCAGACAGTAGTCCTTTCTGTCAGTGTTCGGGAGGTCCTTATGTTGCCTCTGAGAGAACTAATGAAGATAATCATGAAAACAGTGGCCAGAACCATTTCTTATTTGGAAGTGATGGTAACACGGAGGGTGCCACAATAGGAATTTCTAGTTCCAAAAATTTTAAATCTGCAGGAAGTGCTGAGAGTGATGAGGCAGGTCAATTCACCAAATGTCAAGTCAATCATAATACACTCCCGAATATAGATGCAGCAGCAGCAACATGTTCGTTTTCATCAGTTGGTCTTGGCTTCCAACCCAACGGTTGTGCTTCTGAAGCAGCTTCTGTGGATGGAGTAAAAAAGAAAGATGACTTTAGTTTTACTGGCACTACAGATGGATTTGGAGTGTGCTTTGAAGACTTTAAGAGGTCATTTTGTGATCCTTCCTGTCTCAGAGAAAATTTATTTCCTGAATTAAATAAAACTTCAAAATGTGGTGCGAAGGGTAGGTCCTTTAGGGAGAAAAGATCAAGGAAACAAAGGGGAAAGTCAAAGAAACCTTCCATGAGTAAGCCATGGCCCGAACAAGATCATGTGCCTAAGGAAAGTGGTTCCCAAGAAAACCCAGAACCTTCTGGATGCTACTCGCCCATGGATTTCTCTCCCTATGAGGAAACAAGAGTTGCTGATCCACATTCAAGGGGACATTCTGTGACATCTGCTGACCCCTGCGAAACAGTTCCTGTAGATCCCAAAGGTGAAGGTTTGGCTGCTACTGCAAGTGGATTAGGTTATACAGTTGATCAGATATGTAAAGAGCCCATTGAGAAGAATTCGATGTATCATGGTGAGGGAGGTGTTTTTTGTGATGGTTTGTGGAAAGGTTCTGATTCAGAGACAGCTAGAGCTGGTATTGGATTAACTATAGAGAAACAAGAAAGTGATTGTAGATCACCATTTTTTGCTTCGGGTTCCGAAAAAGATAAATGCTTTACGTTTTTGGCATCATCTTCTGTGCAAGGTAGTTCAGTGATGGGTAAGCGCCAACAACGTAGAAAGAAAAACAAGATAAAAGTTGGTCATGCAACATTTGTTATTACTCCAAGTTCAAATGTTGAGTTTGGATCTTTTGGCCTATTTACCCCTCACTCTAGCATCCCTTTATGTACGGAGGTAGTTGGTAAATCAGAAGCAAAAGAACAATTTAAGCAAGTGCATGTCTCTTCTAGTGTTGCTATTCATGAAACCTGTGAGAAATGGCGGATCAGGGGAAATGAAGCTTATAAAAATGTAGATCATTCAAAAGCTGAAGAATTTTACACACAGGGAATAATTTCTATACCTTCTAATGAAAGAGCAGGATGCTCTCTGAAGCCTCTTTTGCTATGCTATAGCAACCGTGCTGCAACGAGAATGTGCCTTGGAAGGATAAGGGAAGCTTTAGGGGACTGTGTGATGGCTACTGCACTAGATCCCAACTTTCTCAAAGCTCAAATGAGAGCTGCAAATTGCCATCTTCTGCTAGGTGAAGTCGAAAATGCAAAGCAGTACTTCAATAAGTGTTCCGAATCAGTAAATGGTGTCTGTTTGGACCGAAGAATTGTAATAAATTCTGCTGATGGCCTACAAAAGATTCAGAAAGTGATTGAGTATACAAATCGTTCTATCAAGCTGCTGGACCAGAGAAATACTGATGCAGCTCTCACTGCATTAGAGCTAATTTCTGAGGCCTTGTCAGTTAGCTCATGCTCAGAGACATTACTTGAAATGAAAGCAGAGGCTTTGTGTTTGTTGCGGAGGTATGAAGAGGCAATTCGTCTGTGTGAGCAGAGTATGGTTTTTGCTGAAAGGAATTTTTCTGGATGTGAAATTTATCAAGTTAAGTTGTGGAGGTGGTTCTTCATATCCAAGTCTCACTTTCATTTGGGAAGGCTTGAGGCAGCCCTTAATTTACTTGAGAAGCTAGAGAAAGTGGGATCTACCAAAGAGATGTATGTAAGTAAGAAACTGGAATCGTCCGTGTCATTGGCTGTCACAATACGTGAACTATTACGCCACAAGAATGCAGGCAATGAAGCATTTAGATTGGGAAGCTATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTAGCAATATGGGATCTCGTCCGTTTTCAGCTATCTGTTTGTGCAACCGCGCTGCAGCACACCAAGCTTTAGGTCAAATTACTGATGCTATTGCAGACTGCAGTCTTGCCATAGCTCTTGATGGCAATTATGTAAAGGCAGTTTCTAGAAGAGCCACCTTACATGAGATGATACGAGACTATGGACAAGCGTCTAGTGATCTCCAAAGACTTGTATCCATTCTAGAAAACCAATCTAATGACATGGCCAAAGAGCCTGGTTCACAAGGTAGATCCAATGGCAGCGTAAAAGAATTACGCAATGCTCGTCGACGTGTGCCTTTGATGGAAGAAGAAGCTAAAAAAGGAATCTCCTTGGATTTTTATCTTATTTTGGGAATTAAACCATCCGATGAGAGTTCTGAAATTAAGAAGGCTTACCGTAAGGCAGCGCTGAAGCATCATCCTGACAAGGCTGGCCAGTTCTTAGCAAGGAGTGAGAGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTGCGGAGGTTCACAAGGATGCTGACAGGCTCTTTAAAATGATTGGAGAGGCATATGCAGTACTTTCAGACCCTACGAAGCGGTCACAGTATGATCTCGAGGAGGAGATGAGAAAAGTAGATATTGAATGCAAGGAAAGCAGCATCTATAGAAAAGCTTCAGGTTTTCAGAGTCCTGGCTGCAGCAGTTATAGAAGACCTGATTTCCGTAGCTCTCCATTTGAGAGAAGCTCTGGTGGTCGAAACTATGGCAGGGAGAGTTGGAGGACATATGGAAATTCGTATCCTCGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ TPR_8

Protein Analysis

1375

Amino Acids

150.51

Weight (kDa)

6.13

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1225 - 1257 5.9e-10 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2469
Acc36I ACCTGC 1 cut(s) 1391
AccB1I GGYRCC 1 cut(s) 1334
AccBSI CCGCTC 2 cut(s) 258, 896
AccI GTMKAC 2 cut(s) 2837, 3625
AccII CGCG 3 cut(s) 181, 196, 3366
AcoI YGGCCR 5 cut(s) 197, 249, 1291, 3561, 3772
AcuI CTGAAG 7 cut(s) 117, 696, 1539, 2514, 2576, 3770, 3972
AfaI GTAC 5 cut(s) 298, 881, 2353, 2735, 3891
AfeI AGCGCT 1 cut(s) 3747
AfiI CCNNNNNNNGG 3 cut(s) 31, 243, 258
AflIII ACRYGT 2 cut(s) 1466, 3628
AhlI ACTAGT 1 cut(s) 299
AjiI CACGTC 1 cut(s) 3629
AjnI CCWGG 2 cut(s) 3571, 4003
AjuI GAANNNNNNNTTGG 2 cut(s) 1091, 1123
Alw26I GTCTC 6 cut(s) 1640, 2075, 2410, 2933, 3102, 3486
AlwNI CAGNNNCTG 3 cut(s) 704, 1529, 1928
Ama87I CYCGRG 1 cut(s) 3926
Aor51HI AGCGCT 1 cut(s) 3747
ArsI GACNNNNNNTTYG 4 cut(s) 1407, 1439, 1679, 1711
Asp700I GAANNNNTTC 5 cut(s) 670, 1301, 1923, 2518, 3985
AspLEI GCGC 3 cut(s) 2229, 3368, 3748
AspS9I GGNCC 7 cut(s) 234, 260, 1241, 1695, 1764, 2776, 2863
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 6 cut(s) 220, 1402, 1955, 2045, 2130, 2723
AsuII TTCGAA 1 cut(s) 926
AvaI CYCGRG 1 cut(s) 3926
AvaII GGWCC 5 cut(s) 234, 1241, 1695, 2776, 2863
AxyI CCTNAGG 1 cut(s) 1785
BalI TGGCCA 3 cut(s) 1293, 3563, 3774
BanI GGYRCC 1 cut(s) 1334
BanII GRGCYC 2 cut(s) 893, 2007
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
BbsI GAAGAC 2 cut(s) 1608, 4030
BbvCI CCTCAGC 1 cut(s) 215
BceAI ACGGC 2 cut(s) 154, 160
BcgI CGANNNNNNTGC 2 cut(s) 1452, 1486
BciT130I CCWGG 2 cut(s) 3573, 4005
BciVI GTATCC 2 cut(s) 3541, 4122
BclI TGATCA 1 cut(s) 1987
BcnI CCSGG 1 cut(s) 264
BcoDI GTCTC 6 cut(s) 1640, 2075, 2410, 2933, 3102, 3486
BcuI ACTAGT 1 cut(s) 299
BfmI CTRYAG 9 cut(s) 1374, 1575, 1929, 2109, 2575, 3369, 3415, 3976, 4009
BfoI RGCGCY 2 cut(s) 2230, 3749
BfuAI ACCTGC 1 cut(s) 1391
BfuI GTATCC 2 cut(s) 3541, 4122
BmcAI AGTACT 2 cut(s) 2735, 3891
Bme1390I CCNGG 3 cut(s) 264, 3573, 4005
Bme18I GGWCC 5 cut(s) 234, 1241, 1695, 2776, 2863
BmeT110I CYCGRG 1 cut(s) 3926
BmgBI CACGTC 1 cut(s) 3629
BmgT120I GGNCC 7 cut(s) 234, 260, 1241, 1695, 1764, 2776, 2863
BmiI GGNNCC 5 cut(s) 892, 1146, 1336, 1798, 2158
BmrFI CCNGG 3 cut(s) 264, 3573, 4005
BmrI ACTGGG 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 252
BoxI GACNNNNGTC 3 cut(s) 1215, 3417, 3505
BpiI GAAGAC 2 cut(s) 1608, 4030
BplI GAGNNNNNCTC 2 cut(s) 4043, 4075
Bpu10I CCTNAGC 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 926
BpuEI CTTGAG 2 cut(s) 3140, 3161
BpuMI CCSGG 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 3227
BsaBI GATNNNNATC 2 cut(s) 792, 3678
BsaJI CCNNGG 6 cut(s) 200, 263, 1760, 1836, 2605, 3669
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 3781, 3811
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 243, 258
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 5 cut(s) 247, 334, 1573, 3198, 3775
Bse21I CCTNAGG 1 cut(s) 1785
Bse3DI GCAATG 3 cut(s) 148, 3262, 3622
Bse8I GATNNNNATC 2 cut(s) 792, 3678
BseBI CCWGG 2 cut(s) 3573, 4005
BseDI CCNNGG 6 cut(s) 200, 263, 1760, 1836, 2605, 3669
BseJI GATNNNNATC 2 cut(s) 792, 3678
BseLI CCNNNNNNNGG 3 cut(s) 31, 243, 258
BseMI GCAATG 3 cut(s) 148, 3262, 3622
BseMII CTCAG 8 cut(s) 206, 1056, 1163, 1248, 1377, 1651, 2901, 2949
BseNI ACTGG 5 cut(s) 247, 334, 1573, 3198, 3775
BseRI GAGGAG 3 cut(s) 63, 3944, 3947
BseX3I CGGCCG 2 cut(s) 197, 249
BsgI GTGCAG 1 cut(s) 2634
Bsh1236I CGCG 3 cut(s) 181, 196, 3366
Bsh1285I CGRYCG 4 cut(s) 185, 194, 200, 252
BshNI GGYRCC 1 cut(s) 1334
BsiEI CGRYCG 4 cut(s) 185, 194, 200, 252
BsiHKCI CYCGRG 1 cut(s) 3926
BsiSI CCGG 4 cut(s) 8, 25, 32, 263
BslFI GGGAC 2 cut(s) 1902, 2645
BslI CCNNNNNNNGG 3 cut(s) 31, 243, 258
BsmAI GTCTC 6 cut(s) 1640, 2075, 2410, 2933, 3102, 3486
BsmFI GGGAC 2 cut(s) 1902, 2645
BsmI GAATGC 6 cut(s) 226, 694, 1105, 1142, 3253, 3962
BsoBI CYCGRG 1 cut(s) 3926
Bsp119I TTCGAA 1 cut(s) 926
Bsp1286I GDGCHC 2 cut(s) 893, 2007
Bsp19I CCATGG 2 cut(s) 1760, 1836
Bsp68I TCGCGA 1 cut(s) 181
BspCNI CTCAG 8 cut(s) 207, 1057, 1164, 1249, 1378, 1650, 2902, 2948
BspFNI CGCG 3 cut(s) 181, 196, 3366
BspHI TCATGA 2 cut(s) 1279, 2425
BspLI GGNNCC 5 cut(s) 892, 1146, 1336, 1798, 2158
BspMAI CTGCAG 4 cut(s) 1378, 3373, 3419, 4013
BspMI ACCTGC 1 cut(s) 1391
BspQI GCTCTTC 1 cut(s) 3460
BspT104I TTCGAA 1 cut(s) 926
BspT107I GGYRCC 1 cut(s) 1334
BsrBI CCGCTC 2 cut(s) 258, 896
BsrDI GCAATG 3 cut(s) 148, 3262, 3622
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 5 cut(s) 247, 334, 1573, 3198, 3775
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 6 cut(s) 200, 263, 1760, 1836, 2605, 3669
BssNAI GTATAC 1 cut(s) 2838
BssT1I CCWWGG 4 cut(s) 1760, 1836, 2605, 3669
Bst1107I GTATAC 1 cut(s) 2838
Bst2UI CCWGG 2 cut(s) 3573, 4005
Bst6I CTCTTC 7 cut(s) 115, 550, 632, 1064, 2413, 2985, 3460
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstBAI YACGTR 1 cut(s) 3227
BstBI TTCGAA 1 cut(s) 926
BstC8I GCNNGC 4 cut(s) 9, 260, 3253, 3772
BstDSI CCRYGG 2 cut(s) 1760, 1836
BstFNI CGCG 3 cut(s) 181, 196, 3366
BstH2I RGCGCY 2 cut(s) 2230, 3749
BstHHI GCGC 3 cut(s) 2229, 3368, 3748
BstMAI GTCTC 6 cut(s) 1640, 2075, 2410, 2933, 3102, 3486
BstMCI CGRYCG 4 cut(s) 185, 194, 200, 252
BstMWI GCNNNNNNNGC 9 cut(s) 220, 1382, 1460, 1520, 1961, 2566, 2891, 3380, 3966
BstNI CCWGG 2 cut(s) 3573, 4005
BstNSI RCATGY 3 cut(s) 103, 1470, 2405
BstPAI GACNNNNGTC 3 cut(s) 1215, 3417, 3505
BstSCI CCNGG 3 cut(s) 262, 3571, 4003
BstSFI CTRYAG 9 cut(s) 1374, 1575, 1929, 2109, 2575, 3369, 3415, 3976, 4009
BstUI CGCG 3 cut(s) 181, 196, 3366
BstV2I GAAGAC 2 cut(s) 1608, 4030
BstX2I RGATCY 6 cut(s) 1933, 2309, 2656, 3161, 3329, 3587
BstYI RGATCY 6 cut(s) 1933, 2309, 2656, 3161, 3329, 3587
BstZ17I GTATAC 1 cut(s) 2838
BstZI CGGCCG 2 cut(s) 197, 249
Bsu36I CCTNAGG 1 cut(s) 1785
BsuI GTATCC 2 cut(s) 3541, 4122
BtgI CCRYGG 2 cut(s) 1760, 1836
BtrI CACGTC 1 cut(s) 3629
BtsI GCAGTG 3 cut(s) 766, 2889, 3297
BtsIMutI CAGTG 6 cut(s) 766, 1235, 1294, 2220, 2889, 3297
BtuMI TCGCGA 1 cut(s) 181
BveI ACCTGC 1 cut(s) 1391
Cac8I GCNNGC 4 cut(s) 9, 260, 3253, 3772
CaiI CAGNNNCTG 3 cut(s) 704, 1529, 1928
CciI TCATGA 2 cut(s) 1279, 2425
CfoI GCGC 3 cut(s) 2229, 3368, 3748
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 7 cut(s) 234, 260, 1241, 1695, 1764, 2776, 2863
CseI GACGC 2 cut(s) 81, 3495
Csp6I GTAC 5 cut(s) 297, 880, 2352, 2734, 3890
CviQI GTAC 5 cut(s) 297, 880, 2352, 2734, 3890
DraI TTTAAA 3 cut(s) 933, 1369, 3865
EaeI YGGCCR 5 cut(s) 197, 249, 1291, 3561, 3772
EagI CGGCCG 2 cut(s) 197, 249
Eam1104I CTCTTC 7 cut(s) 115, 550, 632, 1064, 2413, 2985, 3460
EarI CTCTTC 7 cut(s) 115, 550, 632, 1064, 2413, 2985, 3460
EciI GGCGGA 2 cut(s) 33, 2461
EclXI CGGCCG 2 cut(s) 197, 249
Eco130I CCWWGG 4 cut(s) 1760, 1836, 2605, 3669
Eco147I AGGCCT 1 cut(s) 2915
Eco24I GRGCYC 2 cut(s) 893, 2007
Eco47I GGWCC 5 cut(s) 234, 1241, 1695, 2776, 2863
Eco47III AGCGCT 1 cut(s) 3747
Eco52I CGGCCG 2 cut(s) 197, 249
Eco57I CTGAAG 7 cut(s) 117, 696, 1539, 2514, 2576, 3770, 3972
Eco81I CCTNAGG 1 cut(s) 1785
Eco88I CYCGRG 1 cut(s) 3926
EcoO109I RGGNCCY 2 cut(s) 1241, 1695
EcoRI GAATTC 1 cut(s) 2017
EcoRII CCWGG 2 cut(s) 3571, 4003
EcoT14I CCWWGG 4 cut(s) 1760, 1836, 2605, 3669
EcoT22I ATGCAT 2 cut(s) 777, 1142
EcoT38I GRGCYC 2 cut(s) 893, 2007
ErhI CCWWGG 4 cut(s) 1760, 1836, 2605, 3669
FalI AAGNNNNNCTT 6 cut(s) 2608, 2640, 2720, 2752, 3086, 3118
FaqI GGGAC 2 cut(s) 1902, 2645
FauI CCCGC 2 cut(s) 251, 454
FauNDI CATATG 2 cut(s) 3883, 4099
FbaI TGATCA 1 cut(s) 1987
FblI GTMKAC 2 cut(s) 2837, 3625
FriOI GRGCYC 2 cut(s) 893, 2007
GlaI GCGC 3 cut(s) 2228, 3367, 3747
HaeII RGCGCY 2 cut(s) 2230, 3749
HapII CCGG 4 cut(s) 8, 25, 32, 263
HgaI GACGC 2 cut(s) 81, 3495
HhaI GCGC 3 cut(s) 2229, 3368, 3748
Hin6I GCGC 3 cut(s) 2227, 3366, 3746
HinP1I GCGC 3 cut(s) 2227, 3366, 3746
HincII GTYRAC 2 cut(s) 271, 3626
HindII GTYRAC 2 cut(s) 271, 3626
HindIII AAGCTT 4 cut(s) 2463, 2622, 3381, 3984
HinfI GANTC 9 cut(s) 187, 749, 788, 2075, 2753, 2818, 3197, 3663, 4000
HpaII CCGG 4 cut(s) 8, 25, 32, 263
HphI GGTGA 6 cut(s) 220, 1402, 1955, 2045, 2130, 2723
Hpy166II GTNNAC 6 cut(s) 271, 2838, 3230, 3578, 3626, 3841
Hpy8I GTNNAC 6 cut(s) 271, 2838, 3230, 3578, 3626, 3841
Hpy99I CGWCG 3 cut(s) 181, 3627, 3630
HpyCH4IV ACGT 4 cut(s) 2180, 2236, 3226, 3628
HpyF10VI GCNNNNNNNGC 9 cut(s) 220, 1382, 1460, 1520, 1961, 2566, 2891, 3380, 3966
HpySE526I ACGT 4 cut(s) 2180, 2236, 3226, 3628
HspAI GCGC 3 cut(s) 2227, 3366, 3746
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 1987
LguI GCTCTTC 1 cut(s) 3460
LmnI GCTCC 4 cut(s) 91, 896, 901, 3295
MaeII ACGT 4 cut(s) 2180, 2236, 3226, 3628
MaeIII GTNAC 4 cut(s) 1322, 1897, 3217, 3912
MbiI CCGCTC 2 cut(s) 258, 896
MflI RGATCY 6 cut(s) 1933, 2309, 2656, 3161, 3329, 3587
MhlI GDGCHC 2 cut(s) 893, 2007
MlsI TGGCCA 3 cut(s) 1293, 3563, 3774
MluNI TGGCCA 3 cut(s) 1293, 3563, 3774
MlyI GAGTC 2 cut(s) 181, 4009
MmeI TCCRAC 4 cut(s) 120, 526, 1522, 4070
Mox20I TGGCCA 3 cut(s) 1293, 3563, 3774
Mph1103I ATGCAT 2 cut(s) 777, 1142
MreI CGCCGGCG 1 cut(s) 7
MroNI GCCGGC 1 cut(s) 7
MroXI GAANNNNTTC 5 cut(s) 670, 1301, 1923, 2518, 3985
MscI TGGCCA 3 cut(s) 1293, 3563, 3774
MslI CAYNNNNRTG 2 cut(s) 3246, 3846
Msp20I TGGCCA 3 cut(s) 1293, 3563, 3774
MspI CCGG 4 cut(s) 8, 25, 32, 263
MspR9I CCNGG 3 cut(s) 264, 3573, 4005
Mva1269I GAATGC 6 cut(s) 226, 694, 1105, 1142, 3253, 3962
MvaI CCWGG 2 cut(s) 3573, 4005
MvnI CGCG 3 cut(s) 181, 196, 3366
MwoI GCNNNNNNNGC 9 cut(s) 220, 1382, 1460, 1520, 1961, 2566, 2891, 3380, 3966
NaeI GCCGGC 1 cut(s) 9
NciI CCSGG 1 cut(s) 264
NcoI CCATGG 2 cut(s) 1760, 1836
NdeI CATATG 2 cut(s) 3883, 4099
NgoMIV GCCGGC 1 cut(s) 7
NlaIV GGNNCC 5 cut(s) 892, 1146, 1336, 1798, 2158
NmeAIII GCCGAG 2 cut(s) 225, 277
NmuCI GTSAC 3 cut(s) 1897, 3217, 3912
NruI TCGCGA 1 cut(s) 181
NsiI ATGCAT 2 cut(s) 777, 1142
NspI RCATGY 3 cut(s) 103, 1470, 2405
NspV TTCGAA 1 cut(s) 926
PaeR7I CTCGAG 1 cut(s) 3926
PagI TCATGA 2 cut(s) 1279, 2425
PceI AGGCCT 1 cut(s) 2915
PciI ACATGT 1 cut(s) 1466
PciSI GCTCTTC 1 cut(s) 3460
PcsI WCGNNNNNNNCGW 2 cut(s) 173, 3619
PctI GAATGC 6 cut(s) 226, 694, 1105, 1142, 3253, 3962
PdiI GCCGGC 1 cut(s) 9
PdmI GAANNNNTTC 5 cut(s) 670, 1301, 1923, 2518, 3985
PfeI GAWTC 7 cut(s) 749, 788, 2075, 2753, 2818, 3197, 3663
PflFI GACNNNGTC 1 cut(s) 190
Ple19I CGATCG 1 cut(s) 185
PleI GAGTC 2 cut(s) 181, 4008
PpsI GAGTC 2 cut(s) 181, 4008
Ppu21I YACGTR 1 cut(s) 3227
PpuMI RGGWCCY 2 cut(s) 1241, 1695
PscI ACATGT 1 cut(s) 1466
PshAI GACNNNNGTC 3 cut(s) 1215, 3417, 3505
PsiI TTATAA 1 cut(s) 2469
Psp5II RGGWCCY 2 cut(s) 1241, 1695
Psp6I CCWGG 2 cut(s) 3571, 4003
PspGI CCWGG 2 cut(s) 3571, 4003
PspN4I GGNNCC 5 cut(s) 892, 1146, 1336, 1798, 2158
PspPI GGNCC 7 cut(s) 234, 260, 1241, 1695, 1764, 2776, 2863
PspPPI RGGWCCY 2 cut(s) 1241, 1695
PstI CTGCAG 4 cut(s) 1378, 3373, 3419, 4013
PstNI CAGNNNCTG 3 cut(s) 704, 1529, 1928
PsuI RGATCY 6 cut(s) 1933, 2309, 2656, 3161, 3329, 3587
PsyI GACNNNGTC 1 cut(s) 190
PvuI CGATCG 1 cut(s) 185
RruI TCGCGA 1 cut(s) 181
RsaI GTAC 5 cut(s) 298, 881, 2353, 2735, 3891
RsaNI GTAC 5 cut(s) 297, 880, 2352, 2734, 3890
RseI CAYNNNNRTG 2 cut(s) 3246, 3846
SalI GTCGAC 1 cut(s) 3624
SapI GCTCTTC 1 cut(s) 3460
Sau96I GGNCC 7 cut(s) 234, 260, 1241, 1695, 1764, 2776, 2863
ScaI AGTACT 2 cut(s) 2735, 3891
SchI GAGTC 2 cut(s) 181, 4009
ScrFI CCNGG 3 cut(s) 264, 3573, 4005
SduI GDGCHC 2 cut(s) 893, 2007
SfcI CTRYAG 9 cut(s) 1374, 1575, 1929, 2109, 2575, 3369, 3415, 3976, 4009
Sfr274I CTCGAG 1 cut(s) 3926
SfuI TTCGAA 1 cut(s) 926
SgrAI CRCCGGYG 1 cut(s) 7
SgrDI CGTCGACG 1 cut(s) 3624
SinI GGWCC 5 cut(s) 234, 1241, 1695, 2776, 2863
SlaI CTCGAG 1 cut(s) 3926
SmiI ATTTAAAT 1 cut(s) 933
SmiMI CAYNNNNRTG 2 cut(s) 3246, 3846
SmlI CTYRAG 3 cut(s) 3119, 3140, 3926
SmoI CTYRAG 3 cut(s) 3119, 3140, 3926
SpeI ACTAGT 1 cut(s) 299
SseBI AGGCCT 1 cut(s) 2915
StuI AGGCCT 1 cut(s) 2915
StyD4I CCNGG 3 cut(s) 262, 3571, 4003
StyI CCWWGG 4 cut(s) 1760, 1836, 2605, 3669
SwaI ATTTAAAT 1 cut(s) 933
TaiI ACGT 4 cut(s) 2183, 2239, 3229, 3631
TaqII GACCGA 2 cut(s) 180, 2793
TatI WGTACW 3 cut(s) 879, 2733, 3889
TauI GCSGC 3 cut(s) 14, 49, 199
TfiI GAWTC 7 cut(s) 749, 788, 2075, 2753, 2818, 3197, 3663
TscAI CASTG 6 cut(s) 766, 1235, 1294, 2220, 2896, 3297
TseFI GTSAC 3 cut(s) 1897, 3217, 3912
Tsp45I GTSAC 3 cut(s) 1897, 3217, 3912
TspGWI ACGGA 5 cut(s) 1343, 2369, 3193, 3327, 4025
TspRI CASTG 6 cut(s) 766, 1235, 1294, 2220, 2896, 3297
Tth111I GACNNNGTC 1 cut(s) 190
VpaK11BI GGWCC 5 cut(s) 234, 1241, 1695, 2776, 2863
XbaI TCTAGA 2 cut(s) 3461, 3538
XceI RCATGY 3 cut(s) 103, 1470, 2405
XcmI CCANNNNNNNNNTGG 1 cut(s) 1307
XhoI CTCGAG 1 cut(s) 3926
XmiI GTMKAC 2 cut(s) 2837, 3625
XmnI GAANNNNTTC 5 cut(s) 670, 1301, 1923, 2518, 3985
ZrmI AGTACT 2 cut(s) 2735, 3891
Zsp2I ATGCAT 2 cut(s) 777, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.