FvH4_6g27290

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009615
Physical Location & Seq
Reverse (-)
20918551 .. 20921468
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27290.t2

Sequence Viewer

Length: 372 bp
ATGTTGAGGACGGCTTCATCAGTTTCGGTTCTCCTTCTCACATTCGCAATCGCAATGGCCGACTCTTCTGCTCCCCTCAATCCCGCCTCTTCTGCTTCGGAATCGGCCGTCCACATCGTATACACCGAGAAGCCGCAGCAGAATGAGGAGCCTGAGGCCTATCACATCCGAACCCTCGCCTCCGTCCTCGGCAGTGAGGAGGCTGCCAAGGACGCTCTGGTTTACAGTTACAAGACTGCTGCCAGTGGATTCTCTGCCAAGCTCACTCCTCAACAGGTCGCCGAAATCTCCAAACAACCTGGGGTTCTACAGGTCGTAGCAAGTAGGACACTCCAGCTGCATTCAGGACCTGGGATTGCGAAGCTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9

Protein Analysis

124

Amino Acids

12.93

Weight (kDa)

6.4

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 98 3e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 120
AciI CCGC 2 cut(s) 84, 134
AcoI YGGCCR 2 cut(s) 57, 105
AjnI CCWGG 2 cut(s) 298, 349
AluBI AGCT 3 cut(s) 262, 337, 364
AluI AGCT 3 cut(s) 262, 337, 364
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 3 cut(s) 57, 105, 156
ApeKI GCWGC 5 cut(s) 136, 203, 239, 337, 364
AspS9I GGNCC 1 cut(s) 347
AvaII GGWCC 1 cut(s) 347
AxyI CCTNAGG 1 cut(s) 153
BbvI GCAGC 5 cut(s) 148, 190, 226, 324, 351
BceAI ACGGC 2 cut(s) 27, 92
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 2 cut(s) 300, 351
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 6 cut(s) 134, 137, 204, 240, 338, 365
BlsI GCNGC 6 cut(s) 135, 138, 205, 241, 339, 366
Bme1390I CCNGG 2 cut(s) 300, 351
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 2 cut(s) 300, 351
BpmI CTGGAG 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 187, 207, 299, 350
Bse1I ACTGG 1 cut(s) 243
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 300, 351
BseDI CCNNGG 4 cut(s) 187, 207, 299, 350
BseGI GGATG 1 cut(s) 165
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 3 cut(s) 161, 212, 258
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 5 cut(s) 148, 190, 226, 324, 351
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 59, 107, 158
BsiEI CGRYCG 1 cut(s) 108
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 3 cut(s) 59, 107, 158
BspACI CCGC 2 cut(s) 84, 134
BspANI GGCC 3 cut(s) 59, 107, 158
BspCNI CTCAG 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 4 cut(s) 187, 207, 299, 350
BssNAI GTATAC 1 cut(s) 121
BssT1I CCWWGG 1 cut(s) 207
Bst1107I GTATAC 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 300, 351
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 70, 94
BstDEI CTNAG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 165
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 92, 212
BstNI CCWGG 2 cut(s) 300, 351
BstSCI CCNGG 2 cut(s) 298, 349
BstSFI CTRYAG 1 cut(s) 308
BstV1I GCAGC 5 cut(s) 148, 190, 226, 324, 351
BstZ17I GTATAC 1 cut(s) 121
BstZI CGGCCG 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 153
BsuRI GGCC 3 cut(s) 59, 107, 158
BtsCI GGATG 1 cut(s) 165
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 2 cut(s) 199, 250
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 347
CseI GACGC 1 cut(s) 221
DdeI CTNAG 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 57, 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 2 cut(s) 70, 94
EarI CTCTTC 2 cut(s) 70, 94
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 347
Eco52I CGGCCG 1 cut(s) 105
Eco81I CCTNAGG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 298, 349
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaiI YATR 1 cut(s) 121
FauI CCCGC 1 cut(s) 91
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 6 cut(s) 134, 137, 204, 240, 338, 365
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 6 cut(s) 134, 137, 204, 240, 338, 365
GluI GCNGC 6 cut(s) 134, 137, 204, 240, 338, 365
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 3 cut(s) 59, 107, 158
HgaI GACGC 1 cut(s) 221
HinfI GANTC 3 cut(s) 62, 101, 249
Hpy166II GTNNAC 3 cut(s) 112, 121, 223
Hpy188I TCNGA 2 cut(s) 100, 170
Hpy188III TCNNGA 1 cut(s) 345
Hpy8I GTNNAC 3 cut(s) 112, 121, 223
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 340, 367
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 212
HpyF3I CTNAG 1 cut(s) 153
LmnI GCTCC 2 cut(s) 76, 148
Lsp1109I GCAGC 5 cut(s) 148, 190, 226, 324, 351
MaeIII GTNAC 1 cut(s) 227
MboII GAAGA 2 cut(s) 57, 81
MlyI GAGTC 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 337
MspR9I CCNGG 2 cut(s) 300, 351
Mva1269I GAATGC 1 cut(s) 340
MvaI CCWGG 2 cut(s) 300, 351
MwoI GCNNNNNNNGC 2 cut(s) 92, 212
NlaIV GGNNCC 1 cut(s) 150
NmeAIII GCCGAG 1 cut(s) 168
PceI AGGCCT 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 123
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 101, 249
PkrI GCNGC 6 cut(s) 135, 138, 205, 241, 339, 366
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PpuMI RGGWCCY 1 cut(s) 347
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 2 cut(s) 298, 349
PspGI CCWGG 2 cut(s) 298, 349
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 347
PspPPI RGGWCCY 1 cut(s) 347
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 337
SatI GCNGC 6 cut(s) 134, 137, 204, 240, 338, 365
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 2 cut(s) 300, 351
SetI ASST 7 cut(s) 264, 279, 301, 315, 339, 352, 366
SfcI CTRYAG 1 cut(s) 308
SinI GGWCC 1 cut(s) 347
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 2 cut(s) 84, 134
StuI AGGCCT 1 cut(s) 158
StyD4I CCNGG 2 cut(s) 298, 349
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 227
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 2 cut(s) 101, 249
TscAI CASTG 2 cut(s) 199, 250
TseI GCWGC 5 cut(s) 136, 203, 239, 337, 364
TspDTI ATGAA 1 cut(s) 6
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 2 cut(s) 199, 250
VpaK11BI GGWCC 1 cut(s) 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.