Rorug02G0286600

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009615
Physical Location & Seq
Forward (+)
31252514 .. 31267858
15345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0286600.1

Sequence Viewer

Length: 3273 bp
ATGAGCAACAATGGCAAAACCCGAAAACGGCCGGCACCGCCGCAGCAGCCGTCGCCGGTGAACCCACCACCGCCCAAGCAGCGCGCCGCGTCTCAAGAAGAAGAGGAATTTCTCGACGAGGACGTCTTCCTCGACGAAACCCTACTCGAGACCGAAGACGACCTCATTCTCCGAGACATCGAGCAGCGCCAATCCCTCGCCTCCCGCCTCACCAGGTGGGCCCGCCCACCTCTCTCCGATGCCTACAAGTCCGCCTCCAAAAGCATCCTGTTTCAGCAATTGGAGATTGACTATGTGATTGGAGAGAGCAATAGAGAATTGTTGCCTAATTGGTCCGGTCCTGCTGCCATTATCAGAATATTTGGGGTTACAAAGGAAGGACATAGTGTTTGCTGCCACGTTCATGGATTCGAGCCATATTTCTATATCAACTGCCCTCCAGGGATGGGACATGATGATATTCCTCGGTTTCGTCAGACTCTCGAGGCGAAGATGGGGGAGGCAAACAGGAACAATAAGGCATCAATGTTCATTCGACGTGTTGAAATGGTGTACAAGAGGAATATAATGTATTACCAGCAGCAGGATTCCAATCCCTTTCTCAAAATTGTTGTTTCTTTGCCAACAATGGTGGCCCGCTGCCGGGGTATTCTTGATAAAGGCATACAAATTGATGGTGTTGGTGAGAAGAGCTTCATGACATATGAAAGCAATGTTCTGTTTGCTCTTCGATTCATGATTGATTGCAACATAGTTGGTGGAAATTGGATTGAGGTACCTGCCGGAAAATATAAGACAGCAAAGAGTACATCGTACTGCCAATTAGAGTTCAATTGCCTATATTCAGACTTGATCAGCCATGCTCCTGAAGGTGAATTTTCGAAGATGGCTCCGTTTCGCATTTTAAGCTTTGACATCGAGTGTGCTGGACGCAAAGGTCATTTTCCTGATGCCAAGCATGATCCTGTCATCCAGGTGGCCAACCTAGTTACTTTGCAGGGAGAGAATCAGCCATTTATTCGTAATGTTATGACTCTTAAGTCATGCTCCCCTATAGCTGGTGCTGATGTGATTTCCTTTGATACAGAAAGAGAAGTCTTGCTGGCTTGGAGGGACTTAATCCGTGAGGCAGACCCAGATGTCATAATTGGATATAACATCTGTAAATTTGATTTGCCATATCTCATTGAGAGAGCTGAGACTTTGGGAATACCTGAATTTCCAATACTTGGGCGCATCAGAAACAGCAGGGTTCGGGTCAAAGACACTACTTTTTCTTCAAGGCAGATGGGAACCAGGGAAAGTAAAGAAGTAACAATTGAAGGGAGGGTTCAATTTGATATACTCCAGGTTATGCAACGGGATTATAAATTAAGCTCATATTCATTGAATTCTGTCTCAGCACACTTTCTTTCTGAGCAGAAAGAGGACGTCCACCATTCCATAATATCTGATCTTCAGAATGGCAATGCGGAAACTAGGAGGCGACTTGCTGAGTATTGTCTGAAGGATGCTTACCTCCCGCAGAGGCTTTTGGACAAACTTATGTTTATTTACAATTATGTAGAGATGGCTCGAGTCACTGGTGTTCCCATCTCATTTCTACTTTCCAGAGGGCAGAGCATCAAGGTGCTTTCTCAACTTCTTAGGAAGGCTAAACAAAAGAACCTTCTCCTTCCAAATGTCAAACAGGCTGGATCTGAACAAGGAACATATGAAGGTGCCACTGTTTTGGAAGCAAAGGCAGGATTTTATGAAAAGCCAATTGCAACTTTGGATTTTGCATCACTGTACCCCTCAATCATGATGGCTTATAATCTATGCTATTGCACCCTGGTCCGATCTGAAGATGTACGCAAACTCAACCTTCCTCCAGAATGTGTGAACAAAACTCCATCTGGAGAAACATTTGTTAAACCGAATTTGCAAAAGGGAATTCTTCCTGAAATTCTTGAAGAACTAATAGCTGCTCGGAAGAGAGCAAAAGCGGATTTGAAGGAAGCAAAGGATCCGCTTGAGAAGGCAGTGCTAGATGGTCGACAATTGGCCTTAAAGATAAGTGCAAATTCCGTATATGGGTTTACCGGAGCTACAATTGGTCAGTTACCATGTTTGGAGATATCATCAAGTGTCACGAGCTATGGTCGACAGATGATTGAGCACACAAAAAACCTTGTAAAAGAAAAATTTACTACTCTTGGAGGCTATGAACATAATGCCGAGGTAATATATGGGGACACAGATTCAGTTATGGTGCAATTCGGTGTAAGTACTGTTGAAGCAGCAATGAAATTGGGAAGAGAAGCTGCTGATGCTATCAGTGCCACGTTCACAAAGCCTATCAAACTCGAGTTTGAGAAAGTTTATTATCCATATCTTCTGATTAGCAAGAAGAGATATGCTGGTTTGTTTTGGACAAATCCAGAGAAGTTCGACAAAATGGATACTAAAGGCATTGAAACAGTACGAAGAGACAATTGCTTATTGGTAAAAAACCTGGTAAATGAGTGCTTGCACAAAATACTGATTGACAGAGATATTCCTGGGGCAGTCCAGTTTGTCAAGAACACTATATCTGATCTTCTTATGAACCGCATGGATTTGTCACTCTTGGTTATCACTAAGGGTCTGTCAAAAACTGGGGAGGATTATGGAGTAAAGGCAGCTCATGTTGAACTTGCTGAGCGTATGCGCAAGCGAGATGCTGCCACTGCTCCAACGGTTGGGGATCGAGTACCATATGTCATTATCAAAGCTGCAAAAGGTGCCAAGGCTTATGAGAGGTCAGAGGACCCCATCTATGTGCTCGAGAATAACATTCCTATAGACCCTCAATATTATCTTGAAAACCAAATCAGCAAGCCACTCTTAAGAATTTTTGAGCCTATTTTGAAGAATGCCAGCTCGGAACTTCTCCATGGAAGTCATACAAGATCTATTTCAATGCCTACTCCCTCCAACAGTGGCATAATGAGATTTGCAAAGAAACAACTCACTTGCCTTGGTTGTAAAACTCCAATTAGCAATTCAGACAGAACTCTGTGCTCACACTGTAAGGGAAGAGAAGCTGAACTTTACTGCAAAACTGTGGCTCATGTTTCTGAGCTGGAGACTCTTTTTGCACGTTTATGGACACAGTGCCAGGAGTGCCAAGGTTCACTTCATCAAGATGTGCTCTGCACAAGTCGGGATTGCCCTATATTTTACAGGAGAAAGAAGGCACAAAAAGACATGGGTGAAGCTAAAGTGCAATTAGACCGGTGGAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9

Protein Analysis

1090

Amino Acids

123.26

Weight (kDa)

8.46

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
POL3_N PF24055 136 - 211 6.5e-07 DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1 PF03104 234 - 466 3.2e-71 DNA polymerase family B, exonuclease domain
DNA_pol_B_2 PF03175 472 - 622 5.6e-06 DNA polymerase type B, organellar and viral
DNA_pol_B PF00136 530 - 963 2.6e-163 DNA polymerase family B
zf-C4pol PF14260 1000 - 1071 4.6e-19 C4-type zinc-finger of DNA polymerase delta
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1368, 1815
AasI GACNNNNNNGTC 4 cut(s) 122, 936, 1039, 1139
AatII GACGTC 2 cut(s) 126, 1434
Acc16I TGCGCA 1 cut(s) 2693
Acc36I ACCTGC 1 cut(s) 787
Acc65I GGTACC 1 cut(s) 775
AccB1I GGYRCC 4 cut(s) 34, 775, 1721, 2765
AccB7I CCANNNNNTGG 2 cut(s) 1229, 2723
AccI GTMKAC 2 cut(s) 2038, 2146
AccII CGCG 2 cut(s) 84, 89
AclWI GGATC 5 cut(s) 956, 1705, 2003, 2016, 2736
AcoI YGGCCR 2 cut(s) 29, 978
AcuI CTGAAG 4 cut(s) 888, 1442, 1526, 1866
AcyI GRCGYC 2 cut(s) 123, 1431
AdeI CACNNNGTG 1 cut(s) 216
AfaI GTAC 9 cut(s) 554, 777, 808, 815, 1793, 1854, 2272, 2466, 2736
AfiI CCNNNNNNNGG 9 cut(s) 27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
AflII CTTAAG 2 cut(s) 1037, 2869
AflIII ACRYGT 1 cut(s) 538
AgeI ACCGGT 1 cut(s) 3258
AjiI CACGTC 1 cut(s) 539
AjnI CCWGG 9 cut(s) 212, 439, 972, 1295, 1347, 1833, 2496, 2542, 3141
AjuI GAANNNNNNNTTGG 4 cut(s) 3010, 3042, 3144, 3176
Alw21I GWGCWC 4 cut(s) 2163, 2808, 3047, 3177
Alw26I GTCTC 7 cut(s) 96, 143, 168, 1193, 1402, 2466, 3104
AlwI GGATC 5 cut(s) 956, 1705, 2003, 2016, 2736
Ama87I CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
AoxI GGCC 5 cut(s) 29, 219, 633, 978, 2046
ApaI GGGCCC 1 cut(s) 223
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AsiGI ACCGGT 1 cut(s) 3258
Asp700I GAANNNNTTC 1 cut(s) 692
Asp718I GGTACC 1 cut(s) 775
AspLEI GCGC 5 cut(s) 84, 86, 189, 1236, 2694
AspS9I GGNCC 7 cut(s) 219, 220, 333, 338, 634, 1837, 2791
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 5 cut(s) 70, 202, 695, 884, 3248
AsuII TTCGAA 1 cut(s) 881
AvaI CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
AvaII GGWCC 4 cut(s) 333, 338, 1837, 2791
BaeGI GKGCMC 1 cut(s) 223
BalI TGGCCA 1 cut(s) 980
BamHI GGATCC 1 cut(s) 2008
BanI GGYRCC 4 cut(s) 34, 775, 1721, 2765
BanII GRGCYC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 1499, 1531
BauI CACGAG 1 cut(s) 2134
BbsI GAAGAC 2 cut(s) 118, 162
Bbv12I GWGCWC 4 cut(s) 2163, 2808, 3047, 3177
BceAI ACGGC 2 cut(s) 34, 44
BciT130I CCWGG 9 cut(s) 214, 441, 974, 1297, 1349, 1835, 2498, 2544, 3143
BciVI GTATCC 1 cut(s) 2437
BclI TGATCA 1 cut(s) 852
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 7 cut(s) 96, 143, 168, 1193, 1402, 2466, 3104
BfaI CTAG 3 cut(s) 986, 1479, 2030
BfmI CTRYAG 2 cut(s) 1053, 2823
BfoI RGCGCY 1 cut(s) 190
BfrI CTTAAG 2 cut(s) 1037, 2869
BfuAI ACCTGC 1 cut(s) 787
BfuI GTATCC 1 cut(s) 2437
BglII AGATCT 1 cut(s) 2933
BlpI GCTNAGC 1 cut(s) 2682
BmcAI AGTACT 1 cut(s) 2272
Bme18I GGWCC 4 cut(s) 333, 338, 1837, 2791
BmeT110I CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
BmgBI CACGTC 1 cut(s) 539
BmgT120I GGNCC 7 cut(s) 219, 220, 333, 338, 634, 1837, 2791
BmiI GGNNCC 9 cut(s) 36, 221, 777, 891, 1294, 1723, 2010, 2767, 2793
BmrI ACTGGG 1 cut(s) 2649
BmuI ACTGGG 1 cut(s) 2649
BpiI GAAGAC 2 cut(s) 118, 162
BpmI CTGGAG 5 cut(s) 423, 1331, 1857, 1920, 3128
Bpu1102I GCTNAGC 1 cut(s) 2682
Bpu14I TTCGAA 1 cut(s) 881
BpuEI CTTGAG 2 cut(s) 78, 2036
BpuMI CCSGG 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 2 cut(s) 123, 1431
BsaI GGTCTC 1 cut(s) 143
BsaWI WCCGGW 3 cut(s) 335, 2084, 3258
Bsc4I CCNNNNNNNGG 9 cut(s) 27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
Bse118I RCCGGY 3 cut(s) 31, 55, 3258
Bse1I ACTGG 3 cut(s) 1588, 2554, 2644
Bse3DI GCAATG 3 cut(s) 718, 1474, 2292
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 9 cut(s) 214, 441, 974, 1297, 1349, 1835, 2498, 2544, 3143
BseGI GGATG 4 cut(s) 264, 450, 969, 1516
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 9 cut(s) 27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
BseMI GCAATG 3 cut(s) 718, 1474, 2292
BseMII CTCAG 6 cut(s) 1188, 1407, 1413, 1485, 2673, 3093
BseNI ACTGG 3 cut(s) 1588, 2554, 2644
BsePI GCGCGC 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 223
BseX3I CGGCCG 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 3163
Bsh1236I CGCG 2 cut(s) 84, 89
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 5 cut(s) 31, 221, 635, 980, 2048
BshNI GGYRCC 4 cut(s) 34, 775, 1721, 2765
BshTI ACCGGT 1 cut(s) 3258
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 4 cut(s) 2163, 2808, 3047, 3177
BsiHKCI CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
BsiSI CCGG 7 cut(s) 32, 56, 336, 643, 783, 2085, 3259
BslFI GGGAC 3 cut(s) 462, 1127, 2249
BslI CCNNNNNNNGG 9 cut(s) 27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
BsmAI GTCTC 7 cut(s) 96, 143, 168, 1193, 1402, 2466, 3104
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 3 cut(s) 462, 1127, 2249
BsmI GAATGC 1 cut(s) 2902
BsnI GGCC 5 cut(s) 31, 221, 635, 980, 2048
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
Bsp119I TTCGAA 1 cut(s) 881
Bsp120I GGGCCC 1 cut(s) 219
Bsp1286I GDGCHC 5 cut(s) 223, 2163, 2808, 3047, 3177
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
Bsp1720I GCTNAGC 1 cut(s) 2682
Bsp19I CCATGG 1 cut(s) 2917
BspANI GGCC 5 cut(s) 31, 221, 635, 980, 2048
BspCNI CTCAG 6 cut(s) 1189, 1408, 1412, 1486, 2674, 3094
BspFNI CGCG 2 cut(s) 84, 89
BspHI TCATGA 3 cut(s) 696, 735, 1803
BspLI GGNNCC 9 cut(s) 36, 221, 777, 891, 1294, 1723, 2010, 2767, 2793
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 5 cut(s) 956, 1705, 2003, 2016, 2736
BspQI GCTCTTC 2 cut(s) 683, 732
BspT104I TTCGAA 1 cut(s) 881
BspT107I GGYRCC 4 cut(s) 34, 775, 1721, 2765
BspTI CTTAAG 2 cut(s) 1037, 2869
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 3 cut(s) 718, 1474, 2292
BsrFI RCCGGY 3 cut(s) 31, 55, 3258
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 3 cut(s) 1588, 2554, 2644
BssAI RCCGGY 3 cut(s) 31, 55, 3258
BssHII GCGCGC 1 cut(s) 82
BssMI GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
BssNI GRCGYC 2 cut(s) 123, 1431
BssSI CACGAG 1 cut(s) 2134
BssT1I CCWWGG 4 cut(s) 2769, 2917, 3001, 3151
Bst2BI CACGAG 1 cut(s) 2134
Bst2UI CCWGG 9 cut(s) 214, 441, 974, 1297, 1349, 1835, 2498, 2544, 3143
Bst4CI ACNGT 9 cut(s) 1729, 1791, 2275, 2464, 2722, 2963, 3053, 3088, 3138
Bst6I CTCTTC 8 cut(s) 96, 683, 732, 1970, 2293, 2387, 2464, 3055
BstACI GRCGYC 2 cut(s) 123, 1431
BstAFI CTTAAG 2 cut(s) 1037, 2869
BstAPI GCANNNNNTGC 1 cut(s) 2765
BstAUI TGTACA 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 881
BstC8I GCNNGC 9 cut(s) 33, 84, 223, 637, 1104, 2513, 2696, 2861, 2902
BstDEI CTNAG 8 cut(s) 1197, 1399, 1416, 1494, 1646, 2622, 2682, 3102
BstDSI CCRYGG 1 cut(s) 2917
BstF5I GGATG 4 cut(s) 264, 450, 969, 1516
BstFNI CGCG 2 cut(s) 84, 89
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 5 cut(s) 84, 86, 189, 1236, 2694
BstKTI GATC 9 cut(s) 855, 964, 1456, 1700, 1844, 2011, 2581, 2731, 2936
BstMAI GTCTC 7 cut(s) 96, 143, 168, 1193, 1402, 2466, 3104
BstMBI GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
BstMCI CGRYCG 1 cut(s) 32
BstNI CCWGG 9 cut(s) 214, 441, 974, 1297, 1349, 1835, 2498, 2544, 3143
BstSFI CTRYAG 2 cut(s) 1053, 2823
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 2 cut(s) 84, 89
BstV2I GAAGAC 2 cut(s) 118, 162
BstX2I RGATCY 3 cut(s) 1697, 2008, 2933
BstXI CCANNNNNNTGG 2 cut(s) 404, 1732
BstYI RGATCY 3 cut(s) 1697, 2008, 2933
BstZI CGGCCG 1 cut(s) 29
BsuI GTATCC 1 cut(s) 2437
BsuRI GGCC 5 cut(s) 31, 221, 635, 980, 2048
BtgI CCRYGG 1 cut(s) 2917
BtrI CACGTC 1 cut(s) 539
BtsCI GGATG 4 cut(s) 264, 450, 969, 1516
BtsI GCAGTG 2 cut(s) 2031, 2709
BtsIMutI CAGTG 9 cut(s) 1581, 1725, 1787, 2031, 2326, 2709, 2968, 3049, 3143
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 9 cut(s) 33, 84, 223, 637, 1104, 2513, 2696, 2861, 2902
CciI TCATGA 3 cut(s) 696, 735, 1803
CfoI GCGC 5 cut(s) 84, 86, 189, 1236, 2694
Cfr10I RCCGGY 3 cut(s) 31, 55, 3258
Cfr13I GGNCC 7 cut(s) 219, 220, 333, 338, 634, 1837, 2791
CseI GACGC 2 cut(s) 78, 939
CsiI ACCWGGT 2 cut(s) 212, 2496
Csp6I GTAC 9 cut(s) 553, 776, 807, 814, 1792, 1853, 2271, 2465, 2735
CspAI ACCGGT 1 cut(s) 3258
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviQI GTAC 9 cut(s) 553, 776, 807, 814, 1792, 1853, 2271, 2465, 2735
DdeI CTNAG 8 cut(s) 1197, 1399, 1416, 1494, 1646, 2622, 2682, 3102
DpnI GATC 9 cut(s) 854, 963, 1455, 1699, 1843, 2010, 2580, 2730, 2935
DpnII GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
DraIII CACNNNGTG 1 cut(s) 216
DrdI GACNNNNNNGTC 4 cut(s) 122, 936, 1039, 1139
DseDI GACNNNNNNGTC 4 cut(s) 122, 936, 1039, 1139
EaeI YGGCCR 2 cut(s) 29, 978
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 8 cut(s) 96, 683, 732, 1970, 2293, 2387, 2464, 3055
EarI CTCTTC 8 cut(s) 96, 683, 732, 1970, 2293, 2387, 2464, 3055
EciI GGCGGA 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 4 cut(s) 2769, 2917, 3001, 3151
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 2121
Eco47I GGWCC 4 cut(s) 333, 338, 1837, 2791
Eco52I CGGCCG 1 cut(s) 29
Eco57I CTGAAG 4 cut(s) 888, 1442, 1526, 1866
Eco88I CYCGRG 5 cut(s) 146, 482, 1575, 2348, 2807
EcoO109I RGGNCCY 1 cut(s) 2791
EcoRI GAATTC 2 cut(s) 1390, 1935
EcoRII CCWGG 9 cut(s) 212, 439, 972, 1295, 1347, 1833, 2496, 2542, 3141
EcoRV GATATC 1 cut(s) 2121
EcoT14I CCWWGG 4 cut(s) 2769, 2917, 3001, 3151
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 4 cut(s) 2769, 2917, 3001, 3151
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 6 cut(s) 2852, 2884, 3057, 3089, 3144, 3176
FaqI GGGAC 3 cut(s) 462, 1127, 2249
FauI CCCGC 4 cut(s) 212, 230, 644, 1530
FauNDI CATATG 3 cut(s) 703, 1714, 2740
FbaI TGATCA 1 cut(s) 852
FblI GTMKAC 2 cut(s) 2038, 2146
FokI GGATG 4 cut(s) 251, 457, 956, 1523
FriOI GRGCYC 1 cut(s) 223
FspBI CTAG 3 cut(s) 986, 1479, 2030
FspI TGCGCA 1 cut(s) 2693
GlaI GCGC 5 cut(s) 83, 85, 188, 1235, 2693
GsuI CTGGAG 5 cut(s) 423, 1331, 1857, 1920, 3128
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 5 cut(s) 31, 221, 635, 980, 2048
HapII CCGG 7 cut(s) 32, 56, 336, 643, 783, 2085, 3259
HgaI GACGC 2 cut(s) 78, 939
HhaI GCGC 5 cut(s) 84, 86, 189, 1236, 2694
Hin1I GRCGYC 2 cut(s) 123, 1431
Hin6I GCGC 5 cut(s) 82, 84, 187, 1234, 2692
HinP1I GCGC 5 cut(s) 82, 84, 187, 1234, 2692
HincII GTYRAC 2 cut(s) 2039, 2147
HindII GTYRAC 2 cut(s) 2039, 2147
HindIII AAGCTT 1 cut(s) 907
HinfI GANTC 9 cut(s) 408, 478, 587, 732, 1006, 1033, 1578, 2243, 3112
HpaII CCGG 7 cut(s) 32, 56, 336, 643, 783, 2085, 3259
HphI GGTGA 5 cut(s) 70, 202, 695, 884, 3248
Hpy166II GTNNAC 9 cut(s) 61, 553, 1435, 1885, 2039, 2082, 2147, 2331, 3158
Hpy8I GTNNAC 9 cut(s) 61, 553, 1435, 1885, 2039, 2082, 2147, 2331, 3158
Hpy99I CGWCG 4 cut(s) 55, 119, 137, 540
HpyCH4III ACNGT 9 cut(s) 1729, 1791, 2275, 2464, 2722, 2963, 3053, 3088, 3138
HpyCH4IV ACGT 6 cut(s) 123, 399, 538, 1431, 2327, 3124
HpyF3I CTNAG 8 cut(s) 1197, 1399, 1416, 1494, 1646, 2622, 2682, 3102
HpySE526I ACGT 6 cut(s) 123, 399, 538, 1431, 2327, 3124
Hsp92I GRCGYC 2 cut(s) 123, 1431
HspAI GCGC 5 cut(s) 82, 84, 187, 1234, 2692
KpnI GGTACC 1 cut(s) 779
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 852
Kzo9I GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
LguI GCTCTTC 2 cut(s) 683, 732
LmnI GCTCC 5 cut(s) 868, 895, 1052, 2087, 2719
MabI ACCWGGT 2 cut(s) 212, 2496
MaeI CTAG 3 cut(s) 986, 1479, 2030
MaeII ACGT 6 cut(s) 123, 399, 538, 1431, 2327, 3124
MaeIII GTNAC 7 cut(s) 367, 988, 1312, 1579, 2103, 2131, 2604
MalI GATC 9 cut(s) 854, 963, 1455, 1699, 1843, 2010, 2580, 2730, 2935
MboI GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
MfeI CAATTG 7 cut(s) 278, 832, 1317, 1764, 2042, 2094, 2476
MflI RGATCY 3 cut(s) 1697, 2008, 2933
MhlI GDGCHC 5 cut(s) 223, 2163, 2808, 3047, 3177
MlsI TGGCCA 1 cut(s) 980
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 4 cut(s) 472, 1027, 1587, 3106
MmeI TCCRAC 2 cut(s) 2741, 2982
Mox20I TGGCCA 1 cut(s) 980
MroNI GCCGGC 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 692
MscI TGGCCA 1 cut(s) 980
MseI TTAA 7 cut(s) 905, 1038, 1118, 1373, 1914, 2051, 2870
MslI CAYNNNNRTG 6 cut(s) 402, 974, 1628, 2801, 3127, 3168
Msp20I TGGCCA 1 cut(s) 980
MspA1I CMGCKG 1 cut(s) 639
MspCI CTTAAG 2 cut(s) 1037, 2869
MspI CCGG 7 cut(s) 32, 56, 336, 643, 783, 2085, 3259
MunI CAATTG 7 cut(s) 278, 832, 1317, 1764, 2042, 2094, 2476
Mva1269I GAATGC 1 cut(s) 2902
MvaI CCWGG 9 cut(s) 214, 441, 974, 1297, 1349, 1835, 2498, 2544, 3143
MvnI CGCG 2 cut(s) 84, 89
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 644
NcoI CCATGG 1 cut(s) 2917
NdeI CATATG 3 cut(s) 703, 1714, 2740
NdeII GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
NgoMIV GCCGGC 1 cut(s) 31
NlaIV GGNNCC 9 cut(s) 36, 221, 777, 891, 1294, 1723, 2010, 2767, 2793
NmeAIII GCCGAG 1 cut(s) 2245
NmuCI GTSAC 3 cut(s) 1579, 2131, 2604
NsbI TGCGCA 1 cut(s) 2693
NspV TTCGAA 1 cut(s) 881
PaeR7I CTCGAG 5 cut(s) 146, 482, 1575, 2348, 2807
PagI TCATGA 3 cut(s) 696, 735, 1803
PauI GCGCGC 1 cut(s) 82
PciSI GCTCTTC 2 cut(s) 683, 732
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 129
PctI GAATGC 1 cut(s) 2902
PdiI GCCGGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 692
PfeI GAWTC 5 cut(s) 408, 587, 732, 1006, 2243
PflMI CCANNNNNTGG 2 cut(s) 1229, 2723
PinAI ACCGGT 1 cut(s) 3258
PleI GAGTC 4 cut(s) 472, 1027, 1586, 3106
PpsI GAGTC 4 cut(s) 472, 1027, 1586, 3106
PpuMI RGGWCCY 1 cut(s) 2791
PsiI TTATAA 2 cut(s) 1368, 1815
Psp5II RGGWCCY 1 cut(s) 2791
Psp6I CCWGG 9 cut(s) 212, 439, 972, 1295, 1347, 1833, 2496, 2542, 3141
PspGI CCWGG 9 cut(s) 212, 439, 972, 1295, 1347, 1833, 2496, 2542, 3141
PspN4I GGNNCC 9 cut(s) 36, 221, 777, 891, 1294, 1723, 2010, 2767, 2793
PspOMI GGGCCC 1 cut(s) 219
PspPI GGNCC 7 cut(s) 219, 220, 333, 338, 634, 1837, 2791
PspPPI RGGWCCY 1 cut(s) 2791
PspXI VCTCGAGB 2 cut(s) 1575, 2348
PsuI RGATCY 3 cut(s) 1697, 2008, 2933
PteI GCGCGC 1 cut(s) 82
RsaI GTAC 9 cut(s) 554, 777, 808, 815, 1793, 1854, 2272, 2466, 2736
RsaNI GTAC 9 cut(s) 553, 776, 807, 814, 1792, 1853, 2271, 2465, 2735
RseI CAYNNNNRTG 6 cut(s) 402, 974, 1628, 2801, 3127, 3168
SalI GTCGAC 2 cut(s) 2037, 2145
SapI GCTCTTC 2 cut(s) 683, 732
SaqAI TTAA 7 cut(s) 905, 1038, 1118, 1373, 1914, 2051, 2870
Sau3AI GATC 9 cut(s) 852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
Sau96I GGNCC 7 cut(s) 219, 220, 333, 338, 634, 1837, 2791
ScaI AGTACT 1 cut(s) 2272
SchI GAGTC 4 cut(s) 472, 1027, 1587, 3106
SduI GDGCHC 5 cut(s) 223, 2163, 2808, 3047, 3177
SexAI ACCWGGT 2 cut(s) 212, 2496
SfcI CTRYAG 2 cut(s) 1053, 2823
Sfr274I CTCGAG 5 cut(s) 146, 482, 1575, 2348, 2807
SfuI TTCGAA 1 cut(s) 881
SgrAI CRCCGGYG 1 cut(s) 55
SinI GGWCC 4 cut(s) 333, 338, 1837, 2791
SlaI CTCGAG 5 cut(s) 146, 482, 1575, 2348, 2807
SmiMI CAYNNNNRTG 6 cut(s) 402, 974, 1628, 2801, 3127, 3168
SmlI CTYRAG 9 cut(s) 93, 146, 482, 1037, 1575, 2015, 2348, 2807, 2869
SmoI CTYRAG 9 cut(s) 93, 146, 482, 1037, 1575, 2015, 2348, 2807, 2869
SspI AATATT 2 cut(s) 360, 2837
SspMI CTAG 3 cut(s) 986, 1479, 2030
StyI CCWWGG 4 cut(s) 2769, 2917, 3001, 3151
TaaI ACNGT 9 cut(s) 1729, 1791, 2275, 2464, 2722, 2963, 3053, 3088, 3138
TaiI ACGT 6 cut(s) 126, 402, 541, 1434, 2330, 3127
TaqII GACCGA 1 cut(s) 167
TatI WGTACW 3 cut(s) 552, 806, 2270
TauI GCSGC 2 cut(s) 43, 89
TfiI GAWTC 5 cut(s) 408, 587, 732, 1006, 2243
Tru1I TTAA 7 cut(s) 905, 1038, 1118, 1373, 1914, 2051, 2870
Tru9I TTAA 7 cut(s) 905, 1038, 1118, 1373, 1914, 2051, 2870
TscAI CASTG 9 cut(s) 1588, 1732, 1794, 2031, 2326, 2716, 2968, 3056, 3143
TseFI GTSAC 3 cut(s) 1579, 2131, 2604
Tsp45I GTSAC 3 cut(s) 1579, 2131, 2604
TspGWI ACGGA 3 cut(s) 882, 1112, 2059
TspRI CASTG 9 cut(s) 1588, 1732, 1794, 2031, 2326, 2716, 2968, 3056, 3143
Van91I CCANNNNNTGG 2 cut(s) 1229, 2723
Vha464I CTTAAG 2 cut(s) 1037, 2869
VpaK11BI GGWCC 4 cut(s) 333, 338, 1837, 2791
XhoI CTCGAG 5 cut(s) 146, 482, 1575, 2348, 2807
XmiI GTMKAC 2 cut(s) 2038, 2146
XmnI GAANNNNTTC 1 cut(s) 692
XspI CTAG 3 cut(s) 986, 1479, 2030
ZraI GACGTC 2 cut(s) 124, 1432
ZrmI AGTACT 1 cut(s) 2272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.