MD11G1194700.v1.1

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009615
Physical Location & Seq
Reverse (-)
27909415 .. 27909708
294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1194700.v1.1.491

Sequence Viewer

Length: 294 bp
ATGGTCGACCAATCTGCTCCCAAATCCGTCGCTTTTTCCTCCGAAACGGCGGTCCACATCGTTTACACCAAGCGCCATCTCGAAAATGAGGAGCCCGAGTCCTATCACATCCGAACCCTAGCCTCCGTCCTCGGCAGTGAGGAGGCTGTGAAGGAGGCACTGGTGTACAATTACAAGACGGCTGGCAGTGGATTCTCTGCTAAGCTCACTCCTCAGCAAGTCGAACAAATTTCAACATGCGTAAGGTGTTGCATGTGGGCCCTGAAACCTGCTCAGTGGTATCTAAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9

Protein Analysis

98

Amino Acids

10.89

Weight (kDa)

6.27

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 18 - 78 8.8e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 6
AciI CCGC 1 cut(s) 50
AcsI RAATTY 1 cut(s) 228
AfaI GTAC 1 cut(s) 167
AgsI TTSAA 1 cut(s) 234
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 258
ApaI GGGCCC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 52, 258, 259
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 262
BanII GRGCYC 2 cut(s) 96, 262
BbvCI CCTCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 84
BceAI ACGGC 2 cut(s) 63, 195
BcgI CGANNNNNNTGC 1 cut(s) 30
BfaI CTAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 277
BlpI GCTNAGC 1 cut(s) 201
Bme18I GGWCC 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 3 cut(s) 52, 258, 259
BmiI GGNNCC 2 cut(s) 93, 260
Bpu10I CCTNAGC 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse1I ACTGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 1 cut(s) 108
BseMII CTCAG 2 cut(s) 227, 287
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 3 cut(s) 104, 155, 201
BseSI GKGCMC 1 cut(s) 262
BshFI GGCC 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 95
BsnI GGCC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 95
Bsp120I GGGCCC 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 96, 262
Bsp1407I TGTACA 1 cut(s) 165
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 1 cut(s) 50
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 2 cut(s) 226, 286
BspLI GGNNCC 2 cut(s) 93, 260
BspMI ACCTGC 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 165
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 130
BstAUI TGTACA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 201, 213, 273, 284
BstF5I GGATG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstNSI RCATGY 2 cut(s) 240, 256
BstSLI GKGCMC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 2 cut(s) 142, 193
BtsIMutI CAGTG 4 cut(s) 142, 158, 193, 281
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 52, 258, 259
Csp6I GTAC 1 cut(s) 166
CviAII CATG 2 cut(s) 237, 253
CviJI RGCY 6 cut(s) 94, 122, 146, 182, 205, 260
CviKI_1 RGCY 6 cut(s) 94, 122, 146, 182, 205, 260
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 4 cut(s) 201, 213, 273, 284
Eco24I GRGCYC 2 cut(s) 96, 262
Eco47I GGWCC 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 259
EcoT38I GRGCYC 2 cut(s) 96, 262
FaeI CATG 2 cut(s) 240, 256
FaiI YATR 2 cut(s) 238, 254
FatI CATG 2 cut(s) 236, 252
FblI GTMKAC 1 cut(s) 6
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 96, 262
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 260
HhaI GCGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 240, 256
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 2 cut(s) 98, 192
Hpy166II GTNNAC 4 cut(s) 7, 55, 64, 166
Hpy188I TCNGA 2 cut(s) 43, 113
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 4 cut(s) 7, 55, 64, 166
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 252
HpyF3I CTNAG 4 cut(s) 201, 213, 273, 284
Hsp92II CATG 2 cut(s) 240, 256
HspAI GCGC 1 cut(s) 73
LmnI GCTCC 2 cut(s) 22, 91
LpnPI CCDG 4 cut(s) 146, 168, 275, 282
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 287
MhlI GDGCHC 2 cut(s) 96, 262
MluCI AATT 2 cut(s) 169, 228
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 8 cut(s) 49, 82, 133, 133, 136, 140, 148, 222
NlaIII CATG 2 cut(s) 240, 256
NlaIV GGNNCC 2 cut(s) 93, 260
NmeAIII GCCGAG 1 cut(s) 111
NspI RCATGY 2 cut(s) 240, 256
PfeI GAWTC 1 cut(s) 192
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 93, 260
PspOMI GGGCCC 1 cut(s) 258
PspPI GGNCC 3 cut(s) 52, 258, 259
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
SalI GTCGAC 1 cut(s) 5
Sau96I GGNCC 3 cut(s) 52, 258, 259
SchI GAGTC 1 cut(s) 107
SduI GDGCHC 2 cut(s) 96, 262
SetI ASST 3 cut(s) 207, 248, 271
SinI GGWCC 1 cut(s) 52
Sse9I AATT 2 cut(s) 169, 228
SsiI CCGC 1 cut(s) 50
SspMI CTAG 1 cut(s) 119
TaqI TCGA 3 cut(s) 6, 81, 222
TasI AATT 2 cut(s) 169, 228
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 1 cut(s) 192
TscAI CASTG 4 cut(s) 142, 165, 193, 281
TspGWI ACGGA 2 cut(s) 16, 115
TspRI CASTG 4 cut(s) 142, 165, 193, 281
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 1 cut(s) 228
XceI RCATGY 2 cut(s) 240, 256
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.