Rh7BG090100

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009615
Physical Location & Seq
Forward (+)
6688404 .. 6688973
570 bp
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UTR
Exon/CDS
Intron
Rh7BG090100.1

Sequence Viewer

Length: 264 bp
ATGGCCACAACACCCTCATTGCACCAGTACATTGACGCCGGAAGCAGTCGACTTCACATCGTTTATATGGGTAAAAAGAAGCATGAAGATCCTGAGCTTGTAACTAGTTTACATAATGAGATTCTGGCGTCAATTGTTGGGAGCAAAGAAGCAGCTATTGAATCAATGTTCTATAGCTACAAGCATGGCTTCTCCGGCTTCGCAGCAAAGCTTACAGAATCTCAAGCCAAGGAGATTGCAGATACTTTTACATTGGATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

87

Amino Acids

9.56

Weight (kDa)

5.89

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 19 - 81 2.2e-15 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 49
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 2 cut(s) 36, 128
AfaI GTAC 1 cut(s) 29
AgsI TTSAA 1 cut(s) 161
AhlI ACTAGT 1 cut(s) 104
AluBI AGCT 4 cut(s) 97, 155, 177, 211
AluI AGCT 4 cut(s) 97, 155, 177, 211
AlwI GGATC 1 cut(s) 83
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 152, 203
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 2 cut(s) 164, 215
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 172
BisI GCNGC 2 cut(s) 153, 204
BlsI GCNGC 2 cut(s) 154, 205
BmsI GCATC 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 207
BsaHI GRCGYC 2 cut(s) 36, 128
BsaJI CCNNGG 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 25
Bse3DI GCAATG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 164, 215
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 39, 195
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 88
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 85
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 17
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 1 cut(s) 88
BssNI GRCGYC 2 cut(s) 36, 128
BssT1I CCWWGG 1 cut(s) 228
BstACI GRCGYC 2 cut(s) 36, 128
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 262
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 164, 215
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 262
CseI GACGC 2 cut(s) 44, 117
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 83, 185
CviJI RGCY 8 cut(s) 5, 97, 155, 177, 189, 198, 211, 227
CviKI_1 RGCY 8 cut(s) 5, 97, 155, 177, 189, 198, 211, 227
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 93
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 2 cut(s) 86, 188
FaiI YATR 6 cut(s) 66, 68, 84, 114, 174, 186
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FatI CATG 2 cut(s) 82, 184
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 153, 204
Fsp4HI GCNGC 2 cut(s) 153, 204
FspBI CTAG 1 cut(s) 105
GluI GCNGC 2 cut(s) 153, 204
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 39, 195
HgaI GACGC 2 cut(s) 44, 117
Hin1I GRCGYC 2 cut(s) 36, 128
Hin1II CATG 2 cut(s) 86, 188
HincII GTYRAC 1 cut(s) 50
HindII GTYRAC 1 cut(s) 50
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 3 cut(s) 121, 161, 218
HpaII CCGG 2 cut(s) 39, 195
Hpy166II GTNNAC 2 cut(s) 50, 110
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 2 cut(s) 50, 110
HpyCH4V TGCA 2 cut(s) 22, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 1 cut(s) 93
Hsp92I GRCGYC 2 cut(s) 36, 128
Hsp92II CATG 2 cut(s) 86, 188
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 5 cut(s) 38, 52, 105, 110, 208
Lsp1109I GCAGC 2 cut(s) 164, 215
LweI GCATC 1 cut(s) 247
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 100
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 1 cut(s) 98
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 88
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 132
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 25
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 39, 195
MunI CAATTG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 195
NdeII GATC 1 cut(s) 88
NlaIII CATG 2 cut(s) 86, 188
PfeI GAWTC 3 cut(s) 121, 161, 218
PkrI GCNGC 2 cut(s) 154, 205
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SalI GTCGAC 1 cut(s) 48
SatI GCNGC 2 cut(s) 153, 204
Sau3AI GATC 1 cut(s) 88
SetI ASST 4 cut(s) 99, 157, 179, 213
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 172
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 1 cut(s) 132
SspMI CTAG 1 cut(s) 105
StyI CCWWGG 1 cut(s) 228
TaqI TCGA 1 cut(s) 49
TasI AATT 1 cut(s) 132
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 3 cut(s) 121, 161, 218
TseI GCWGC 2 cut(s) 152, 203
TspDTI ATGAA 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.