pycom10g03410

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009615
Physical Location & Seq
Forward (+)
3724779 .. 3725195
417 bp
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UTR
Exon/CDS
Intron
pycom10g03410.1

Sequence Viewer

Length: 312 bp
ATGGTAAGAATCACCAAGGCTTTCCTGTCTTCAATTTTGCTTCTGAATCTCATCATCTTCTCTGTGTTCGCCATGGCCGACCAATCTGCTCCCAAATCCGTCGCTGCTTCCTCCGAAACGGCGGTCCACATCGTTTACACCGAACGCCCTCTCGAAAATGAAGAGCCCGAGTCCTATCACATCCGAACCCTAGCCTCCGTCCTCGGCAGTGAGGAGGCTGCCAAGGAGGCGCTGGTATACAGTTACAAGACGGCTGCCAGTGGATTCTCTGCTAAGCTCACTCCTCAGCAGGTTGAACAAATTTCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9

Protein Analysis

104

Amino Acids

11.18

Weight (kDa)

5.28

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 43 - 102 1.1e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 237
AciI CCGC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 300
AgsI TTSAA 3 cut(s) 33, 296, 306
AluBI AGCT 1 cut(s) 277
AluI AGCT 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 104, 218, 254
ApoI RAATTY 1 cut(s) 300
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 91, 205, 241
BceAI ACGGC 2 cut(s) 135, 267
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 280
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 3 cut(s) 105, 219, 255
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 3 cut(s) 106, 220, 256
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 285
Bpu1102I GCTNAGC 1 cut(s) 273
BsaJI CCNNGG 4 cut(s) 15, 72, 202, 222
Bse1I ACTGG 1 cut(s) 258
BseDI CCNNGG 4 cut(s) 15, 72, 202, 222
BseGI GGATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 258
BseRI GAGGAG 2 cut(s) 227, 273
BseXI GCAGC 3 cut(s) 91, 205, 241
BshFI GGCC 1 cut(s) 77
BsiHKCI CYCGRG 1 cut(s) 167
BsnI GGCC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 1 cut(s) 122
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 258
BssECI CCNNGG 4 cut(s) 15, 72, 202, 222
BssNAI GTATAC 1 cut(s) 238
BssT1I CCWWGG 3 cut(s) 15, 72, 222
Bst1107I GTATAC 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 156
BstDEI CTNAG 2 cut(s) 273, 285
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 91, 205, 241
BstV2I GAAGAC 1 cut(s) 21
BstZ17I GTATAC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 265
BveI ACCTGC 1 cut(s) 280
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 124
CviAII CATG 1 cut(s) 73
CviJI RGCY 7 cut(s) 20, 77, 166, 194, 218, 254, 277
CviKI_1 RGCY 7 cut(s) 20, 77, 166, 194, 218, 254, 277
DdeI CTNAG 2 cut(s) 273, 285
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 15, 72, 222
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 167
EcoT14I CCWWGG 3 cut(s) 15, 72, 222
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 3 cut(s) 15, 72, 222
FaeI CATG 1 cut(s) 76
FaiI YATR 2 cut(s) 74, 238
FatI CATG 1 cut(s) 72
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 105, 219, 255
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 105, 219, 255
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 231
GluI GCNGC 3 cut(s) 105, 219, 255
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 1 cut(s) 77
HhaI GCGC 1 cut(s) 232
Hin1II CATG 1 cut(s) 76
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 4 cut(s) 9, 46, 170, 264
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 127, 136, 238
Hpy188I TCNGA 3 cut(s) 45, 115, 185
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 3 cut(s) 127, 136, 238
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 2 cut(s) 273, 285
Hsp92II CATG 1 cut(s) 76
HspAI GCGC 1 cut(s) 230
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 4 cut(s) 38, 218, 271, 275
Lsp1109I GCAGC 3 cut(s) 91, 205, 241
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 242
MboII GAAGA 3 cut(s) 21, 49, 173
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 2 cut(s) 33, 300
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 8 cut(s) 121, 159, 205, 205, 208, 212, 220, 294
MwoI GCNNNNNNNGC 1 cut(s) 227
NcoI CCATGG 1 cut(s) 72
NlaIII CATG 1 cut(s) 76
NmeAIII GCCGAG 1 cut(s) 183
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 138
PfeI GAWTC 3 cut(s) 9, 46, 264
PkrI GCNGC 3 cut(s) 106, 220, 256
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 156
SatI GCNGC 3 cut(s) 105, 219, 255
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 1 cut(s) 168
SetI ASST 2 cut(s) 279, 294
SinI GGWCC 1 cut(s) 124
Sse9I AATT 2 cut(s) 33, 300
SsiI CCGC 1 cut(s) 122
SspMI CTAG 1 cut(s) 191
StyI CCWWGG 3 cut(s) 15, 72, 222
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 153
TasI AATT 2 cut(s) 33, 300
TfiI GAWTC 3 cut(s) 9, 46, 264
TscAI CASTG 2 cut(s) 214, 265
TseI GCWGC 3 cut(s) 104, 218, 254
TspDTI ATGAA 1 cut(s) 174
TspGWI ACGGA 2 cut(s) 88, 187
TspRI CASTG 2 cut(s) 214, 265
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 300
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.