Rroxscaffold_3G00267160

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009615
Physical Location & Seq
Reverse (-)
60242512 .. 60243050
539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267160.1

Sequence Viewer

Length: 333 bp
ATGGGTCAAAAGCAGCATGAAGATCCTGAGCTTGTAACTAGTTTACATAATGAGATTCTGGCGTCAGTTGTTGGGAGCAAAGAAGCAGCTATTGAATCAATGTTCTATAGCTACAAGCATGGCTTCTCCAGCTTCGCAGCAAAGCTTACAGAATCTCAAGCCAAGGAGATTGCAGATACTTTTTACATTGGATGCGTAAGCCTCATAGGGCATGCCATTTTTCTATCAATGTATAATGATTGGAATTCCTTCTGTTGTATCTCATTAGAGTTATCTGGTGTTGTTCATGTCATGCCCAATAGCTTTCATAAGCTCCAAACTACAAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

110

Amino Acids

12.24

Weight (kDa)

5.79

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 3 - 59 2.3e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 62
AgsI TTSAA 1 cut(s) 95
AhlI ACTAGT 1 cut(s) 38
AluBI AGCT 7 cut(s) 31, 89, 111, 132, 145, 303, 313
AluI AGCT 7 cut(s) 31, 89, 111, 132, 145, 303, 313
AlwI GGATC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 13, 86, 137
ApoI RAATTY 1 cut(s) 244
Asp700I GAANNNNTTC 1 cut(s) 248
BbvI GCAGC 3 cut(s) 25, 98, 149
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 3 cut(s) 14, 87, 138
BlsI GCNGC 3 cut(s) 15, 88, 139
BmsI GCATC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 112
Bpu10I CCTNAGC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 18
BseXI GCAGC 3 cut(s) 25, 98, 149
Bsp143I GATC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 22
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 197
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 215
BstSFI CTRYAG 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 25, 98, 149
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BtsCI GGATG 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 213
CseI GACGC 1 cut(s) 51
CviAII CATG 5 cut(s) 17, 119, 212, 287, 292
DdeI CTNAG 1 cut(s) 27
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 5 cut(s) 20, 122, 215, 290, 295
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 5 cut(s) 16, 118, 211, 286, 291
Fnu4HI GCNGC 3 cut(s) 14, 87, 138
FokI GGATG 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 14, 87, 138
FspBI CTAG 1 cut(s) 39
GluI GCNGC 3 cut(s) 14, 87, 138
GsuI CTGGAG 1 cut(s) 112
HgaI GACGC 1 cut(s) 51
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 5 cut(s) 20, 122, 215, 290, 295
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 3 cut(s) 55, 95, 152
Hpy166II GTNNAC 1 cut(s) 44
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 259
HpyCH4V TGCA 1 cut(s) 173
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 5 cut(s) 20, 122, 215, 290, 295
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 2 cut(s) 75, 318
LpnPI CCDG 4 cut(s) 39, 44, 142, 261
Lsp1109I GCAGC 3 cut(s) 25, 98, 149
LweI GCATC 1 cut(s) 182
MaeI CTAG 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 1 cut(s) 32
MflI RGATCY 1 cut(s) 22
MluCI AATT 1 cut(s) 244
MnlI CCTC 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 129
NdeII GATC 1 cut(s) 22
NlaIII CATG 5 cut(s) 20, 122, 215, 290, 295
NspI RCATGY 1 cut(s) 215
PaeI GCATGC 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 55, 95, 152
PkrI GCNGC 3 cut(s) 15, 88, 139
PsuI RGATCY 1 cut(s) 22
SatI GCNGC 3 cut(s) 14, 87, 138
Sau3AI GATC 1 cut(s) 22
SetI ASST 7 cut(s) 33, 91, 113, 134, 147, 305, 315
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
SpeI ACTAGT 1 cut(s) 38
SphI GCATGC 1 cut(s) 215
Sse9I AATT 1 cut(s) 244
SspMI CTAG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 162
TasI AATT 1 cut(s) 244
TfiI GAWTC 3 cut(s) 55, 95, 152
TseI GCWGC 3 cut(s) 13, 86, 137
TspDTI ATGAA 3 cut(s) 33, 275, 296
XapI RAATTY 1 cut(s) 244
XceI RCATGY 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.