FvH4_6g46180

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013702
Physical Location & Seq
Reverse (-)
35273125 .. 35275321
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46180.t1

Sequence Viewer

Length: 1098 bp
ATGAGAATCATGGGAATCAAACTATCAGTTTCATCACTTACTTTTCTGTTCTTGAGTTTTGCAAATTTCAGCTCAGTTTCAGTAGAAGGATCTCCGATTGCACCAGCATTATACGTGTTTGGGGATTCTTTGTTTGATAGTGGTAACAATAATTTCTTGCCAACTTTAGCAAGGGCAAATTTCCTGCCCTATGGTACCAATTTTCCGAAGGGTGCCACCGGAAGATTCACCAACGGTAAAACAGTTGCAGATTTTATAGCTGAGTTTCTTGGGCTACCATACGCTCCACCAAACTTGAGCATACACAAATCAACAATACTTACCGGCCTGAATTATGCATCTGGGTCTTGTGGCATTCTCCCGGAAACTGGAAGCCGATTGGGGGAGTGCTTGAATCTGAAAGAGCAATTGGATTTGTTTGAAAGAACAGTGAAGTCTGATTTGTCAGGACAATTCAACAATTCAAATGATCTTGCAGAGTACTTGTCCAAGTCCATATTTTTATTTTCTGTAGGCAACAATGATTATCTTAATAACTACCTTCAACCTAAACTTTATGACACAAGCAAACGCTACCCTCCACCACAGTTTGCACAGCTCTTGATGGATGATCTTTCTCACCATTTTGAGAGATTATATAACCTAGGAGCCAGGAAAACAGTTATGTTTGAAATTGGTCCCCTTGGTTGCATCCCATCAATTGCAAAGACAGAAAAACACGATGGAGACTGTGTGCAAGGAACAAACCAGCTCGCTTCGATTTTTAACGAAAGACTTCGTGTAACTCTCGCAAATTTAACGTTCACTTTCCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGGTATGATGCAATCACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCAAGACTTGGAACAATGGGACATCGTCATGTATTCCATTTGCTAAACCTTGCTCTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTTACTGAGTCTGCTAATTCAGTCATAGCAACTGGCTGTTTCAATGGTTCAACTATTTGCACTCCACTAAACATCCAGAAACTTGTACAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

39.95

Weight (kDa)

6.59

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 344 4.5e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 887
Acc65I GGTACC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 194, 212
AclI AACGTT 1 cut(s) 800
AclWI GGATC 3 cut(s) 97, 823, 854
AcsI RAATTY 3 cut(s) 64, 178, 793
AfaI GTAC 3 cut(s) 196, 482, 1089
AfiI CCNNNNNNNGG 2 cut(s) 368, 382
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 8 cut(s) 394, 422, 457, 465, 545, 671, 1045, 1053
AjnI CCWGG 1 cut(s) 650
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 5 cut(s) 72, 260, 598, 751, 839
AluI AGCT 5 cut(s) 72, 260, 598, 751, 839
Alw26I GTCTC 1 cut(s) 720
AlwI GGATC 3 cut(s) 97, 823, 854
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 2 cut(s) 325, 883
ApeKI GCWGC 1 cut(s) 909
ApoI RAATTY 3 cut(s) 64, 178, 793
Asp700I GAANNNNTTC 1 cut(s) 774
Asp718I GGTACC 1 cut(s) 194
AspA2I CCTAGG 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 677
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 220, 611
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 1 cut(s) 677
AvrII CCTAGG 1 cut(s) 643
BanI GGYRCC 2 cut(s) 194, 212
BbvI GCAGC 1 cut(s) 896
BccI CCATC 3 cut(s) 598, 703, 716
BciT130I CCWGG 1 cut(s) 652
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 720
BfaI CTAG 1 cut(s) 644
BfmI CTRYAG 1 cut(s) 510
BisI GCNGC 1 cut(s) 910
BlnI CCTAGG 1 cut(s) 643
BlsI GCNGC 1 cut(s) 911
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 2 cut(s) 362, 652
Bme18I GGWCC 1 cut(s) 677
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 4 cut(s) 196, 214, 649, 679
BmrFI CCNGG 2 cut(s) 362, 652
BmsI GCATC 3 cut(s) 347, 699, 847
BpuEI CTTGAG 2 cut(s) 73, 316
BpuMI CCSGG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 643, 682, 811
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 382
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 373, 1039
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 3 cut(s) 643, 682, 811
BseGI GGATG 3 cut(s) 613, 690, 1074
BseLI CCNNNNNNNGG 2 cut(s) 368, 382
BseMII CTCAG 3 cut(s) 87, 252, 999
BseNI ACTGG 2 cut(s) 373, 1039
BseXI GCAGC 1 cut(s) 896
Bsh1285I CGRYCG 1 cut(s) 835
BshFI GGCC 2 cut(s) 327, 885
BshNI GGYRCC 2 cut(s) 194, 212
BsiEI CGRYCG 1 cut(s) 835
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 3 cut(s) 219, 324, 362
BslFI GGGAC 4 cut(s) 663, 855, 943, 1004
BslI CCNNNNNNNGG 2 cut(s) 368, 382
BsmAI GTCTC 1 cut(s) 720
BsmFI GGGAC 4 cut(s) 663, 855, 943, 1004
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 2 cut(s) 327, 885
BsoBI CYCGRG 1 cut(s) 887
Bsp1407I TGTACA 1 cut(s) 1087
Bsp143I GATC 5 cut(s) 89, 469, 610, 815, 846
BspANI GGCC 2 cut(s) 327, 885
BspCNI CTCAG 3 cut(s) 86, 253, 1000
BspLI GGNNCC 4 cut(s) 196, 214, 649, 679
BspPI GGATC 3 cut(s) 97, 823, 854
BspT107I GGYRCC 2 cut(s) 194, 212
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 1087
BsrI ACTGG 2 cut(s) 373, 1039
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 643, 682, 811
BssMI GATC 5 cut(s) 89, 469, 610, 815, 846
BssT1I CCWWGG 3 cut(s) 643, 682, 811
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 6 cut(s) 236, 244, 430, 588, 661, 731
BstAPI GCANNNNNTGC 1 cut(s) 906
BstAUI TGTACA 1 cut(s) 1087
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 753
BstDEI CTNAG 3 cut(s) 73, 261, 1008
BstF5I GGATG 3 cut(s) 613, 690, 1074
BstKTI GATC 5 cut(s) 92, 472, 613, 818, 849
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 5 cut(s) 89, 469, 610, 815, 846
BstMCI CGRYCG 1 cut(s) 835
BstMWI GCNNNNNNNGC 1 cut(s) 906
BstNI CCWGG 1 cut(s) 652
BstSCI CCNGG 2 cut(s) 360, 650
BstSFI CTRYAG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 896
BstX2I RGATCY 2 cut(s) 89, 815
BstYI RGATCY 2 cut(s) 89, 815
BsuRI GGCC 2 cut(s) 327, 885
BtsCI GGATG 3 cut(s) 613, 690, 1074
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 753
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 677
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 3 cut(s) 195, 481, 1088
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 3 cut(s) 10, 906, 939
CviQI GTAC 3 cut(s) 195, 481, 1088
DdeI CTNAG 3 cut(s) 73, 261, 1008
DpnI GATC 5 cut(s) 91, 471, 612, 817, 848
DpnII GATC 5 cut(s) 89, 469, 610, 815, 846
Eco130I CCWWGG 3 cut(s) 643, 682, 811
Eco47I GGWCC 1 cut(s) 677
Eco88I CYCGRG 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 650
EcoT14I CCWWGG 3 cut(s) 643, 682, 811
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 643, 682, 811
FaeI CATG 3 cut(s) 13, 909, 942
FaqI GGGAC 4 cut(s) 663, 855, 943, 1004
FatI CATG 3 cut(s) 9, 905, 938
Fnu4HI GCNGC 1 cut(s) 910
FokI GGATG 3 cut(s) 620, 677, 1061
Fsp4HI GCNGC 1 cut(s) 910
FspBI CTAG 1 cut(s) 644
GluI GCNGC 1 cut(s) 910
HaeIII GGCC 2 cut(s) 327, 885
HapII CCGG 3 cut(s) 219, 324, 362
HgaI GACGC 1 cut(s) 1001
Hin1II CATG 3 cut(s) 13, 909, 942
HinfI GANTC 6 cut(s) 6, 15, 125, 225, 394, 1010
HpaII CCGG 3 cut(s) 219, 324, 362
HphI GGTGA 2 cut(s) 220, 611
Hpy166II GTNNAC 1 cut(s) 804
Hpy188I TCNGA 4 cut(s) 96, 207, 399, 439
Hpy188III TCNNGA 4 cut(s) 52, 447, 601, 1078
Hpy8I GTNNAC 1 cut(s) 804
HpyAV CCTTC 3 cut(s) 80, 202, 551
HpyCH4III ACNGT 6 cut(s) 236, 244, 430, 588, 661, 731
HpyCH4IV ACGT 2 cut(s) 114, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 906
HpyF3I CTNAG 3 cut(s) 73, 261, 1008
HpySE526I ACGT 2 cut(s) 114, 800
Hsp92II CATG 3 cut(s) 13, 909, 942
KpnI GGTACC 1 cut(s) 198
Kzo9I GATC 5 cut(s) 89, 469, 610, 815, 846
LmnI GCTCC 2 cut(s) 289, 647
Lsp1109I GCAGC 1 cut(s) 896
LweI GCATC 3 cut(s) 347, 699, 847
MaeI CTAG 1 cut(s) 644
MaeII ACGT 2 cut(s) 114, 800
MaeIII GTNAC 3 cut(s) 143, 781, 977
MalI GATC 5 cut(s) 91, 471, 612, 817, 848
MboI GATC 5 cut(s) 89, 469, 610, 815, 846
MboII GAAGA 2 cut(s) 234, 976
MfeI CAATTG 2 cut(s) 407, 699
MflI RGATCY 2 cut(s) 89, 815
MlyI GAGTC 1 cut(s) 1019
MnlI CCTC 4 cut(s) 588, 883, 884, 896
Mph1103I ATGCAT 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 3 cut(s) 531, 765, 797
MslI CAYNNNNRTG 1 cut(s) 937
MspI CCGG 3 cut(s) 219, 324, 362
MspR9I CCNGG 2 cut(s) 362, 652
MunI CAATTG 2 cut(s) 407, 699
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 652
MwoI GCNNNNNNNGC 1 cut(s) 906
NciI CCSGG 1 cut(s) 362
NdeII GATC 5 cut(s) 89, 469, 610, 815, 846
NlaIII CATG 3 cut(s) 13, 909, 942
NlaIV GGNNCC 4 cut(s) 196, 214, 649, 679
NmuCI GTSAC 1 cut(s) 977
NsiI ATGCAT 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 887
PctI GAATGC 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 5 cut(s) 6, 15, 125, 225, 394
PflFI GACNNNGTC 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 1 cut(s) 911
PleI GAGTC 1 cut(s) 1018
PpsI GAGTC 1 cut(s) 1018
Ppu21I YACGTR 1 cut(s) 115
Psp1406I AACGTT 1 cut(s) 800
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 4 cut(s) 196, 214, 649, 679
PspPI GGNCC 1 cut(s) 677
PspXI VCTCGAGB 1 cut(s) 887
PsuI RGATCY 2 cut(s) 89, 815
PsyI GACNNNGTC 1 cut(s) 934
RsaI GTAC 3 cut(s) 196, 482, 1089
RsaNI GTAC 3 cut(s) 195, 481, 1088
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 3 cut(s) 531, 765, 797
SatI GCNGC 1 cut(s) 910
Sau3AI GATC 5 cut(s) 89, 469, 610, 815, 846
Sau96I GGNCC 1 cut(s) 677
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 2 cut(s) 362, 652
SfaNI GCATC 3 cut(s) 347, 699, 847
SfcI CTRYAG 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 887
SinI GGWCC 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 3 cut(s) 52, 295, 887
SmoI CTYRAG 3 cut(s) 52, 295, 887
SspMI CTAG 1 cut(s) 644
StyD4I CCNGG 2 cut(s) 360, 650
StyI CCWWGG 3 cut(s) 643, 682, 811
TaaI ACNGT 6 cut(s) 236, 244, 430, 588, 661, 731
TaiI ACGT 2 cut(s) 117, 803
TaqI TCGA 3 cut(s) 758, 835, 888
TaqII GACCGA 1 cut(s) 821
TatI WGTACW 2 cut(s) 480, 1087
TfiI GAWTC 5 cut(s) 6, 15, 125, 225, 394
Tru1I TTAA 3 cut(s) 531, 765, 797
Tru9I TTAA 3 cut(s) 531, 765, 797
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 1 cut(s) 909
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 2 cut(s) 21, 989
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 934
VpaK11BI GGWCC 1 cut(s) 677
XapI RAATTY 3 cut(s) 64, 178, 793
XhoI CTCGAG 1 cut(s) 887
XmaJI CCTAGG 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 1 cut(s) 644
ZrmI AGTACT 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.