MD09G1076300.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013702
Physical Location & Seq
Forward (+)
5388791 .. 5390406
1616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1076300.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGTTCCAGAAAACGAGAATGAAATCAGTGTCGCTTATTTTCTTGTTTATAATTATTTTCTTGCATTTTCACTCATTGCAGATGCATAGTTTGCCAATTGCACCAGCGCTGTATGTGTTTGGGGATTCACTTTTTGACAGTGGTAACAATAACTTTTTACCAACCATTTGCAAGGCAGACTACTTGCCTTATGGGGTGAATTTTGTTAAGGGAGTCACTGGAAGATTTACCAACGGAAGAACAGTAGCAGATTTTATAGCTGAGTTTCTTGGCCTACCATATCCTCCCCCATACTTGAGCATACGTAGATCGGCGACAGCGCTTACAGGCCTGAATTATGCATCTGGATCTTGTGGCATTCTGCCAGAAACTGGAAGCCAATATGGAAAATGTTTGAATCTAAAAGATCAGATGGATTTGTTTCAACGAACCGTGAAGTCAGACTTGCCAGGACAAATAAAAAATCAAACTGATCTTCTGCAGTACTTGTCCAAATCTATATTTCTGTTTTCTGTTGGCAGCAATGATTTCATCAACAACTACCTCGAACATGAAGTTTTCGATACAAGCAAACGCTACTCTCCTCAACAATTTGCACAGCGCTTAATGGATACAATTGCTCATCATATGGAGAGGTTATACAACTTAGGAGCCAGGAAAGTAGTCATGTTTGAAATTGGTCCGCTTGGATGCATTCCATCCATAGCAAAAACACACAAACACAGTGGAAACTGTGTGGAAAGTACAAATGAGCTAGCCTCGATTTTCAATGACAAGCTTCGTGCAACACTTGCGAATTTAACCACCACTTTCCAAGGTTCTTTCTTTGTTCTCGGTCGAGCAAACTGGATTGGCTATGATGCAATTACAAGTCCTGTTAAATATGGGCTAATGGATGGAAGCAATCCATGCTGTACTACTTGGGAAAATGGGACTTCAGGGTGCATTCCATTGCTAACACCGTGCTTCGAACCAAATAACCACTTCTTTTGGGATGCTTATCATCTAACGGAATATGCAGGTTCAGTCATTGCAACAGGCTGCTTCAATGGCTCAACTGTTTGCACCCCTTTAAACATTAAGCAACTTGTGAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.79

Weight (kDa)

8.23

Isoelectric Point (pI)

28.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 345 1.1e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 1010
AccB7I CCANNNNNTGG 1 cut(s) 371
AciI CCGC 1 cut(s) 683
AclWI GGATC 1 cut(s) 355
AcsI RAATTY 2 cut(s) 199, 796
AcuI CTGAAG 1 cut(s) 921
AfaI GTAC 3 cut(s) 485, 745, 916
AfeI AGCGCT 3 cut(s) 108, 321, 602
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 5 cut(s) 397, 425, 674, 769, 1048
AjnI CCWGG 2 cut(s) 448, 653
AluBI AGCT 3 cut(s) 260, 754, 778
AluI AGCT 3 cut(s) 260, 754, 778
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 371
Aor51HI AGCGCT 3 cut(s) 108, 321, 602
AoxI GGCC 2 cut(s) 271, 328
ApeKI GCWGC 2 cut(s) 519, 1041
ApoI RAATTY 2 cut(s) 199, 796
AspLEI GCGC 3 cut(s) 109, 322, 603
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 208
AsuII TTCGAA 1 cut(s) 969
AsuNHI GCTAGC 1 cut(s) 754
AvaII GGWCC 1 cut(s) 680
BbvI GCAGC 2 cut(s) 531, 1028
BccI CCATC 3 cut(s) 406, 706, 890
BciT130I CCWGG 2 cut(s) 450, 655
BciVI GTATCC 1 cut(s) 604
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 1 cut(s) 479
BfoI RGCGCY 3 cut(s) 110, 323, 604
BfuAI ACCTGC 1 cut(s) 1010
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 2 cut(s) 520, 1042
BlsI GCNGC 2 cut(s) 521, 1043
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 2 cut(s) 450, 655
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 2 cut(s) 450, 655
BmsI GCATC 5 cut(s) 72, 350, 680, 850, 985
BmtI GCTAGC 1 cut(s) 758
BplI GAGNNNNNCTC 2 cut(s) 743, 775
Bpu14I TTCGAA 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 316
BsaAI YACGTR 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 1 cut(s) 814
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 3 cut(s) 223, 376, 851
Bse3DI GCAATG 4 cut(s) 74, 529, 950, 1029
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 2 cut(s) 450, 655
BseDI CCNNGG 1 cut(s) 814
BseGI GGATG 4 cut(s) 695, 698, 901, 1000
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 4 cut(s) 74, 529, 950, 1029
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 3 cut(s) 223, 376, 851
BseRI GAGGAG 1 cut(s) 573
BseXI GCAGC 2 cut(s) 531, 1028
Bsh1285I CGRYCG 1 cut(s) 838
BshFI GGCC 2 cut(s) 273, 330
BsiEI CGRYCG 1 cut(s) 838
BslFI GGGAC 1 cut(s) 946
BslI CCNNNNNNNGG 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 946
BsmI GAATGC 3 cut(s) 357, 693, 945
BsnI GGCC 2 cut(s) 273, 330
Bsp119I TTCGAA 1 cut(s) 969
Bsp143I GATC 4 cut(s) 308, 347, 406, 472
BspACI CCGC 1 cut(s) 683
BspANI GGCC 2 cut(s) 273, 330
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 652
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 1010
BspOI GCTAGC 1 cut(s) 758
BspPI GGATC 1 cut(s) 355
BspT104I TTCGAA 1 cut(s) 969
BsrDI GCAATG 4 cut(s) 74, 529, 950, 1029
BsrI ACTGG 3 cut(s) 223, 376, 851
BssECI CCNNGG 1 cut(s) 814
BssMI GATC 4 cut(s) 308, 347, 406, 472
BssT1I CCWWGG 1 cut(s) 814
Bst2UI CCWGG 2 cut(s) 450, 655
Bst4CI ACNGT 7 cut(s) 140, 244, 433, 725, 734, 963, 1060
BstAPI GCANNNNNTGC 3 cut(s) 91, 791, 909
BstBAI YACGTR 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 2 cut(s) 261, 646
BstF5I GGATG 4 cut(s) 695, 698, 901, 1000
BstH2I RGCGCY 3 cut(s) 110, 323, 604
BstHHI GCGC 3 cut(s) 109, 322, 603
BstKTI GATC 4 cut(s) 311, 350, 409, 475
BstMBI GATC 4 cut(s) 308, 347, 406, 472
BstMCI CGRYCG 1 cut(s) 838
BstMWI GCNNNNNNNGC 4 cut(s) 91, 791, 909, 1050
BstNI CCWGG 2 cut(s) 450, 655
BstSCI CCNGG 2 cut(s) 448, 653
BstSFI CTRYAG 1 cut(s) 479
BstSNI TACGTA 1 cut(s) 305
BstV1I GCAGC 2 cut(s) 531, 1028
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 2 cut(s) 273, 330
BtsCI GGATG 4 cut(s) 695, 698, 901, 1000
BtsIMutI CAGTG 4 cut(s) 33, 145, 216, 730
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 1 cut(s) 756
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 3 cut(s) 109, 322, 603
Cfr13I GGNCC 1 cut(s) 680
Csp6I GTAC 3 cut(s) 484, 744, 915
CspCI CAANNNNNGTGG 4 cut(s) 706, 741, 971, 1006
CviAII CATG 3 cut(s) 551, 667, 909
CviQI GTAC 3 cut(s) 484, 744, 915
DdeI CTNAG 2 cut(s) 261, 646
DpnI GATC 4 cut(s) 310, 349, 408, 474
DpnII GATC 4 cut(s) 308, 347, 406, 472
DraI TTTAAA 1 cut(s) 1074
Eco105I TACGTA 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 814
Eco147I AGGCCT 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 680
Eco47III AGCGCT 3 cut(s) 108, 321, 602
Eco57I CTGAAG 1 cut(s) 921
EcoRII CCWGG 2 cut(s) 448, 653
EcoT14I CCWWGG 1 cut(s) 814
EcoT22I ATGCAT 3 cut(s) 87, 343, 695
ErhI CCWWGG 1 cut(s) 814
FaeI CATG 3 cut(s) 554, 670, 912
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 1 cut(s) 946
FatI CATG 3 cut(s) 550, 666, 908
FauNDI CATATG 1 cut(s) 627
Fnu4HI GCNGC 2 cut(s) 520, 1042
FokI GGATG 4 cut(s) 685, 702, 908, 1007
Fsp4HI GCNGC 2 cut(s) 520, 1042
FspBI CTAG 1 cut(s) 755
GlaI GCGC 3 cut(s) 108, 321, 602
GluI GCNGC 2 cut(s) 520, 1042
HaeII RGCGCY 3 cut(s) 110, 323, 604
HaeIII GGCC 2 cut(s) 273, 330
HhaI GCGC 3 cut(s) 109, 322, 603
Hin1II CATG 3 cut(s) 554, 670, 912
Hin6I GCGC 3 cut(s) 107, 320, 601
HinP1I GCGC 3 cut(s) 107, 320, 601
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 3 cut(s) 125, 213, 397
HphI GGTGA 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 411, 442
Hpy188III TCNNGA 2 cut(s) 7, 345
HpyCH4III ACNGT 7 cut(s) 140, 244, 433, 725, 734, 963, 1060
HpyCH4IV ACGT 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 791, 909, 1050
HpyF3I CTNAG 2 cut(s) 261, 646
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 3 cut(s) 554, 670, 912
HspAI GCGC 3 cut(s) 107, 320, 601
Kzo9I GATC 4 cut(s) 308, 347, 406, 472
LmnI GCTCC 1 cut(s) 650
Lsp1109I GCAGC 2 cut(s) 531, 1028
LweI GCATC 5 cut(s) 72, 350, 680, 850, 985
MaeI CTAG 1 cut(s) 755
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 143, 214
MalI GATC 4 cut(s) 310, 349, 408, 474
MboI GATC 4 cut(s) 308, 347, 406, 472
MboII GAAGA 3 cut(s) 234, 249, 467
MfeI CAATTG 2 cut(s) 96, 615
MflI RGATCY 1 cut(s) 347
MluCI AATT 9 cut(s) 51, 96, 199, 334, 590, 615, 675, 796, 864
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 5 cut(s) 294, 554, 594, 627, 769
Mph1103I ATGCAT 3 cut(s) 87, 343, 695
MseI TTAA 6 cut(s) 207, 605, 800, 879, 1073, 1080
MspR9I CCNGG 2 cut(s) 450, 655
MunI CAATTG 2 cut(s) 96, 615
Mva1269I GAATGC 3 cut(s) 357, 693, 945
MvaI CCWGG 2 cut(s) 450, 655
MwoI GCNNNNNNNGC 4 cut(s) 91, 791, 909, 1050
NdeI CATATG 1 cut(s) 627
NdeII GATC 4 cut(s) 308, 347, 406, 472
NheI GCTAGC 1 cut(s) 754
NlaIII CATG 3 cut(s) 554, 670, 912
NlaIV GGNNCC 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 3 cut(s) 87, 343, 695
NspV TTCGAA 1 cut(s) 969
PceI AGGCCT 1 cut(s) 330
PctI GAATGC 3 cut(s) 357, 693, 945
PfeI GAWTC 2 cut(s) 125, 397
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 2 cut(s) 521, 1043
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 305
PsiI TTATAA 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 448, 653
PspGI CCWGG 2 cut(s) 448, 653
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 3 cut(s) 485, 745, 916
RsaNI GTAC 3 cut(s) 484, 744, 915
SaqAI TTAA 6 cut(s) 207, 605, 800, 879, 1073, 1080
SatI GCNGC 2 cut(s) 520, 1042
Sau3AI GATC 4 cut(s) 308, 347, 406, 472
Sau96I GGNCC 1 cut(s) 680
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 2 cut(s) 450, 655
SetI ASST 8 cut(s) 262, 307, 546, 638, 756, 780, 820, 1024
SfaNI GCATC 5 cut(s) 72, 350, 680, 850, 985
SfcI CTRYAG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 969
SinI GGWCC 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
SnaBI TACGTA 1 cut(s) 305
Sse9I AATT 9 cut(s) 51, 96, 199, 334, 590, 615, 675, 796, 864
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 1 cut(s) 683
SspMI CTAG 1 cut(s) 755
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 448, 653
StyI CCWWGG 1 cut(s) 814
TaaI ACNGT 7 cut(s) 140, 244, 433, 725, 734, 963, 1060
TaiI ACGT 1 cut(s) 307
TaqI TCGA 5 cut(s) 546, 561, 761, 838, 969
TaqII GACCGA 1 cut(s) 824
TasI AATT 9 cut(s) 51, 96, 199, 334, 590, 615, 675, 796, 864
TatI WGTACW 3 cut(s) 483, 743, 914
TfiI GAWTC 2 cut(s) 125, 397
Tru1I TTAA 6 cut(s) 207, 605, 800, 879, 1073, 1080
Tru9I TTAA 6 cut(s) 207, 605, 800, 879, 1073, 1080
TscAI CASTG 4 cut(s) 33, 145, 223, 730
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 519, 1041
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 3 cut(s) 35, 520, 567
TspGWI ACGGA 2 cut(s) 249, 1025
TspRI CASTG 4 cut(s) 33, 145, 223, 730
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 2 cut(s) 199, 796
XspI CTAG 1 cut(s) 755
ZrmI AGTACT 1 cut(s) 485
Zsp2I ATGCAT 3 cut(s) 87, 343, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.