Rh2BG588400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013702
Physical Location & Seq
Reverse (-)
80490563 .. 80494062
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG588400.1

Sequence Viewer

Length: 750 bp
ATGCAAGAGCCGGTAAACTGGTCTCATTCAGCCGGGAAGTGCTTGAATCTGAAAGAGCAGATGGATTTGTTTGAAAAAACGGTGAGGTCAGACTTGTCACGACATTTCAAAAATCCCAATGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACCTTCAACCTAAACTCTATAACACAAGCAAACGCTACCCTTCTCCACAGTTTGCACAACTCTTGATGGATAATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCACCCCATCAATTGCAAAGACACAAAGCCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTCGCCTCGATTTTTAACGACAAACTTCGTGCAACTCTCGCAAATTTAACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCACAACTTGGAACAATGGGACATCGGCATGTATTCCATGGGCTAAACCATGCTTTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTGACTGAATCTGCTAGTTCAGTCATAGCAACTGGCTGTTTCAATGATACAACTGTTTGCACTCCACTAAACATCAAGAAACTTGTACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

249

Amino Acids

27.73

Weight (kDa)

8.34

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 17 - 228 1.6e-17 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 539
AciI CCGC 2 cut(s) 128, 386
AclWI GGATC 2 cut(s) 475, 506
AcsI RAATTY 1 cut(s) 445
AfaI GTAC 2 cut(s) 134, 741
AgsI TTSAA 8 cut(s) 46, 74, 109, 197, 323, 464, 620, 697
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 2 cut(s) 403, 491
AluI AGCT 2 cut(s) 403, 491
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 2 cut(s) 475, 506
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 1 cut(s) 561
ApoI RAATTY 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 94, 263
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 329
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BbvI GCAGC 1 cut(s) 548
BccI CCATC 3 cut(s) 55, 250, 355
BciT130I CCWGG 1 cut(s) 304
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 162
BisI GCNGC 1 cut(s) 562
BlsI GCNGC 1 cut(s) 563
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 34, 304
Bme18I GGWCC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 301, 331
BmrFI CCNGG 2 cut(s) 34, 304
BmsI GCATC 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 334, 599
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 23, 691
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 334, 599
BseNI ACTGG 2 cut(s) 23, 691
BseXI GCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 547
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 537
BsiEI CGRYCG 1 cut(s) 487
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 11, 33
BslFI GGGAC 4 cut(s) 315, 507, 595, 656
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 4 cut(s) 315, 507, 595, 656
BsnI GGCC 1 cut(s) 537
Bso31I GGTCTC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 739
Bsp143I GATC 3 cut(s) 121, 467, 498
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 2 cut(s) 128, 386
BspANI GGCC 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 301, 331
BspPI GGATC 2 cut(s) 475, 506
BspTNI GGTCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 739
BsrI ACTGG 2 cut(s) 23, 691
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 334, 599
BssMI GATC 3 cut(s) 121, 467, 498
BssT1I CCWWGG 2 cut(s) 334, 599
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 4 cut(s) 82, 240, 383, 709
BstAPI GCANNNNNTGC 1 cut(s) 558
BstAUI TGTACA 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 295
BstDSI CCRYGG 1 cut(s) 599
BstKTI GATC 3 cut(s) 124, 470, 501
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 3 cut(s) 121, 467, 498
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 2 cut(s) 440, 558
BstNI CCWGG 1 cut(s) 304
BstNSI RCATGY 1 cut(s) 594
BstSCI CCNGG 2 cut(s) 32, 302
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 1 cut(s) 537
BtgI CCRYGG 1 cut(s) 599
Cac8I GCNNGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 653
Csp6I GTAC 2 cut(s) 133, 740
CviAII CATG 4 cut(s) 558, 591, 600, 612
CviQI GTAC 2 cut(s) 133, 740
DdeI CTNAG 1 cut(s) 295
DpnI GATC 3 cut(s) 123, 469, 500
DpnII GATC 3 cut(s) 121, 467, 498
Eco130I CCWWGG 2 cut(s) 334, 599
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 334, 599
ErhI CCWWGG 2 cut(s) 334, 599
FaeI CATG 4 cut(s) 561, 594, 603, 615
FaqI GGGAC 4 cut(s) 315, 507, 595, 656
FatI CATG 4 cut(s) 557, 590, 599, 611
Fnu4HI GCNGC 1 cut(s) 562
Fsp4HI GCNGC 1 cut(s) 562
FspBI CTAG 1 cut(s) 669
GluI GCNGC 1 cut(s) 562
HaeIII GGCC 1 cut(s) 537
HapII CCGG 2 cut(s) 11, 33
HgaI GACGC 1 cut(s) 653
Hin1II CATG 4 cut(s) 561, 594, 603, 615
HinfI GANTC 2 cut(s) 46, 662
HpaII CCGG 2 cut(s) 11, 33
HphI GGTGA 2 cut(s) 94, 263
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 3 cut(s) 51, 91, 657
Hpy188III TCNNGA 3 cut(s) 99, 253, 730
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 203, 240, 383
HpyCH4III ACNGT 4 cut(s) 82, 240, 383, 709
HpyCH4V TGCA 8 cut(s) 4, 245, 342, 356, 434, 512, 564, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 558
HpyF3I CTNAG 1 cut(s) 295
Hsp92II CATG 4 cut(s) 561, 594, 603, 615
Kzo9I GATC 3 cut(s) 121, 467, 498
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 7 cut(s) 4, 24, 46, 289, 316, 625, 672
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 499
MaeI CTAG 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 96, 629
MalI GATC 3 cut(s) 123, 469, 500
MboI GATC 3 cut(s) 121, 467, 498
MboII GAAGA 1 cut(s) 628
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 1 cut(s) 467
MluCI AATT 5 cut(s) 187, 324, 351, 445, 513
MnlI CCTC 5 cut(s) 78, 418, 535, 536, 548
MseI TTAA 3 cut(s) 183, 417, 449
MslI CAYNNNNRTG 1 cut(s) 589
MspI CCGG 2 cut(s) 11, 33
MspR9I CCNGG 2 cut(s) 34, 304
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 440, 558
NciI CCSGG 1 cut(s) 34
NcoI CCATGG 1 cut(s) 599
NdeII GATC 3 cut(s) 121, 467, 498
NlaIII CATG 4 cut(s) 561, 594, 603, 615
NlaIV GGNNCC 2 cut(s) 301, 331
NmuCI GTSAC 2 cut(s) 96, 629
NspI RCATGY 1 cut(s) 594
PaeR7I CTCGAG 1 cut(s) 539
PfeI GAWTC 2 cut(s) 46, 662
PkrI GCNGC 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 301, 331
PspPI GGNCC 1 cut(s) 329
PspXI VCTCGAGB 1 cut(s) 539
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 2 cut(s) 134, 741
RsaNI GTAC 2 cut(s) 133, 740
RseI CAYNNNNRTG 1 cut(s) 589
SaqAI TTAA 3 cut(s) 183, 417, 449
SatI GCNGC 1 cut(s) 562
Sau3AI GATC 3 cut(s) 121, 467, 498
Sau96I GGNCC 1 cut(s) 329
ScaI AGTACT 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 34, 304
SetI ASST 5 cut(s) 89, 195, 202, 405, 493
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 162
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 589
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 5 cut(s) 187, 324, 351, 445, 513
SsiI CCGC 2 cut(s) 128, 386
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 32, 302
StyI CCWWGG 2 cut(s) 334, 599
TaaI ACNGT 4 cut(s) 82, 240, 383, 709
TaqI TCGA 3 cut(s) 410, 487, 540
TaqII GACCGA 1 cut(s) 473
TasI AATT 5 cut(s) 187, 324, 351, 445, 513
TatI WGTACW 2 cut(s) 132, 739
TfiI GAWTC 2 cut(s) 46, 662
Tru1I TTAA 3 cut(s) 183, 417, 449
Tru9I TTAA 3 cut(s) 183, 417, 449
TseFI GTSAC 2 cut(s) 96, 629
TseI GCWGC 1 cut(s) 561
Tsp45I GTSAC 2 cut(s) 96, 629
TspDTI ATGAA 1 cut(s) 641
TspGWI ACGGA 1 cut(s) 561
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 1 cut(s) 445
XceI RCATGY 1 cut(s) 594
XhoI CTCGAG 1 cut(s) 539
XspI CTAG 1 cut(s) 669
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.