RLG00000021471

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013702
Physical Location & Seq
Reverse (-)
75702334 .. 75703292
959 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021471

Sequence Viewer

Length: 690 bp
ATGGATTTGTTTGAAAAAACGGTGAGGTCAGACTTGTCACGACATTTCAAAAATCCCAATGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACCTTCAACCTAAACTCTATAACACAAGCAAGCGCTACCCTCCTCCACAGTTTGCACAACTCTTGATGGATAATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCACCCCATCAATTGCAAAGACACAAAACCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTCGCCTCGATTTTTAACGACAAACTTCGTGCAACTCTCGCAAATTTGACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCATAACTTGGAACAATGGGACATCGGCATGTATTCCATGGGCTAAACCGTGCTTTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTTACTGAATCTGCTAGTTCAGTCATAGCAACTGGCTGTTTCAATGATACAACTGTTTGCACTCCACTAAACATCAAGAAACTTGTACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

230

Amino Acids

25.5

Weight (kDa)

8.39

Isoelectric Point (pI)

22.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 24 - 208 1.9e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 479
AciI CCGC 2 cut(s) 68, 326
AclWI GGATC 2 cut(s) 415, 446
AcsI RAATTY 1 cut(s) 385
AfaI GTAC 2 cut(s) 74, 681
AfeI AGCGCT 1 cut(s) 164
AgsI TTSAA 7 cut(s) 14, 49, 137, 263, 404, 560, 637
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 2 cut(s) 343, 431
AluI AGCT 2 cut(s) 343, 431
AlwI GGATC 2 cut(s) 415, 446
Ama87I CYCGRG 1 cut(s) 479
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 1 cut(s) 475
ApeKI GCWGC 1 cut(s) 501
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 2 cut(s) 34, 203
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 269
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BbvI GCAGC 1 cut(s) 488
BccI CCATC 2 cut(s) 190, 295
BciT130I CCWGG 1 cut(s) 244
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 166
BisI GCNGC 1 cut(s) 502
BlsI GCNGC 1 cut(s) 503
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 2 cut(s) 241, 271
BmrFI CCNGG 1 cut(s) 244
BmsI GCATC 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 274, 539
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bse1I ACTGG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 274, 539
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 488
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 479
BslFI GGGAC 4 cut(s) 255, 447, 535, 596
BsmFI GGGAC 4 cut(s) 255, 447, 535, 596
BsnI GGCC 1 cut(s) 477
BsoBI CYCGRG 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 679
Bsp143I GATC 3 cut(s) 61, 407, 438
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 2 cut(s) 68, 326
BspANI GGCC 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 241, 271
BspPI GGATC 2 cut(s) 415, 446
BsrGI TGTACA 1 cut(s) 679
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 2 cut(s) 274, 539
BssMI GATC 3 cut(s) 61, 407, 438
BssT1I CCWWGG 2 cut(s) 274, 539
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 5 cut(s) 22, 180, 323, 552, 649
BstAPI GCANNNNNTGC 1 cut(s) 498
BstAUI TGTACA 1 cut(s) 679
BstC8I GCNNGC 2 cut(s) 161, 345
BstDEI CTNAG 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 539
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 64, 410, 441
BstMBI GATC 3 cut(s) 61, 407, 438
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 2 cut(s) 380, 498
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
BsuRI GGCC 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 539
Cac8I GCNNGC 2 cut(s) 161, 345
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 73, 680
CviAII CATG 3 cut(s) 498, 531, 540
CviJI RGCY 7 cut(s) 242, 343, 431, 444, 477, 545, 630
CviKI_1 RGCY 7 cut(s) 242, 343, 431, 444, 477, 545, 630
CviQI GTAC 2 cut(s) 73, 680
DdeI CTNAG 1 cut(s) 235
DpnI GATC 3 cut(s) 63, 409, 440
DpnII GATC 3 cut(s) 61, 407, 438
Eco130I CCWWGG 2 cut(s) 274, 539
Eco47I GGWCC 1 cut(s) 269
Eco47III AGCGCT 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 274, 539
ErhI CCWWGG 2 cut(s) 274, 539
FaeI CATG 3 cut(s) 501, 534, 543
FaqI GGGAC 4 cut(s) 255, 447, 535, 596
FatI CATG 3 cut(s) 497, 530, 539
Fnu4HI GCNGC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 502
FspBI CTAG 1 cut(s) 609
GlaI GCGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 502
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 477
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 165
Hin1II CATG 3 cut(s) 501, 534, 543
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 1 cut(s) 602
HphI GGTGA 2 cut(s) 34, 203
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 3 cut(s) 39, 193, 670
HpyAV CCTTC 2 cut(s) 143, 323
HpyCH4III ACNGT 5 cut(s) 22, 180, 323, 552, 649
HpyCH4V TGCA 7 cut(s) 185, 282, 296, 374, 452, 504, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 498
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 3 cut(s) 501, 534, 543
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 3 cut(s) 61, 407, 438
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 4 cut(s) 229, 256, 565, 612
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 36, 569
MalI GATC 3 cut(s) 63, 409, 440
MboI GATC 3 cut(s) 61, 407, 438
MboII GAAGA 1 cut(s) 568
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 407
MluCI AATT 5 cut(s) 127, 264, 291, 385, 453
MnlI CCTC 7 cut(s) 18, 180, 183, 358, 475, 476, 488
MseI TTAA 2 cut(s) 123, 357
MslI CAYNNNNRTG 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 244
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 380, 498
NcoI CCATGG 1 cut(s) 539
NdeII GATC 3 cut(s) 61, 407, 438
NlaIII CATG 3 cut(s) 501, 534, 543
NlaIV GGNNCC 2 cut(s) 241, 271
NmuCI GTSAC 2 cut(s) 36, 569
NspI RCATGY 1 cut(s) 534
PaeR7I CTCGAG 1 cut(s) 479
PfeI GAWTC 1 cut(s) 602
PkrI GCNGC 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 241, 271
PspPI GGNCC 1 cut(s) 269
PspXI VCTCGAGB 1 cut(s) 479
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 2 cut(s) 74, 681
RsaNI GTAC 2 cut(s) 73, 680
RseI CAYNNNNRTG 1 cut(s) 529
SaqAI TTAA 2 cut(s) 123, 357
SatI GCNGC 1 cut(s) 502
Sau3AI GATC 3 cut(s) 61, 407, 438
Sau96I GGNCC 1 cut(s) 269
ScaI AGTACT 1 cut(s) 74
ScrFI CCNGG 1 cut(s) 244
SetI ASST 5 cut(s) 29, 135, 142, 345, 433
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 5 cut(s) 127, 264, 291, 385, 453
SsiI CCGC 2 cut(s) 68, 326
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 2 cut(s) 274, 539
TaaI ACNGT 5 cut(s) 22, 180, 323, 552, 649
TaqI TCGA 3 cut(s) 350, 427, 480
TaqII GACCGA 1 cut(s) 413
TasI AATT 5 cut(s) 127, 264, 291, 385, 453
TatI WGTACW 2 cut(s) 72, 679
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 2 cut(s) 123, 357
Tru9I TTAA 2 cut(s) 123, 357
TseFI GTSAC 2 cut(s) 36, 569
TseI GCWGC 1 cut(s) 501
Tsp45I GTSAC 2 cut(s) 36, 569
TspDTI ATGAA 1 cut(s) 581
TspGWI ACGGA 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 385
XceI RCATGY 1 cut(s) 534
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.