pycom17g06730

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013702
Physical Location & Seq
Forward (+)
4870795 .. 4872020
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g06730.1

Sequence Viewer

Length: 771 bp
ATGTTGCAGAATATGAGAATGGAATCAATGCCACTTATATTCTTGTTCACATTTATTTTGTTGCATCTTCACTCACTGCAGATGCATAGTTTGCCAATTGCACCAGCGCTGTATGTGTTTGGGGATTCACTGTTTGACGGTGGTAACAATAATTTTTTGCCAACTATTTGTAAGGCAGACTATCTGCCTTATGGGGTGAATTTTGTCGAGGGAGCCACTGGAAGATTTACCAATGGCAGAACAGTAGCAGATTTTATAGCTGAGTTTCTTGGCCTACCATATCCTCCCCCATACATGAGCATACTTGGATCGGCGACGGTGCTTAAAGGCCTAAATTATGCATCTGACTCTTGTGGCATTCTACCAGAAACTGGAAGCCAATGTGGTACATGCTTAAATCTAAAAGACCAGATAGATTTGTTTCAACGAAAGGTGAAGTCAGACTTGCCAGGACAAATAAAAAATCACACTGATCTTTTGCAGCACTTGTCCAAATCTTTATTTCTGTTTTCTGTTGGCAGCAATGATTTCATCTACAACTACCTCGAACCTGCAGTTTTCGATACAAGCAAACGCTACTCTCCTCAACAATTTGTACAAGTCTTGATGGATGCAATTGCTCACCATATGGAGAAAATGTTGCAGACGCTATACAATTTAGGAGTCAGGAAAATAGTCATGTTTGAAATTGGTCCGCTTGGATGCATCCCGTCAATTGCAAAAACACATAATCACAGTGGAAACTGTGTGGAACATACAAATGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

257

Amino Acids

28.55

Weight (kDa)

6.18

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 252 2.2e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 371
AciI CCGC 1 cut(s) 695
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 199
AfaI GTAC 2 cut(s) 388, 597
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 2 cut(s) 425, 686
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 2 cut(s) 260, 768
AluI AGCT 2 cut(s) 260, 768
AlwI GGATC 1 cut(s) 316
AlwNI CAGNNNCTG 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 2 cut(s) 271, 328
ApeKI GCWGC 2 cut(s) 481, 519
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 692
AsuHPI GGTGA 3 cut(s) 208, 445, 614
AvaII GGWCC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 493, 531
BccI CCATC 1 cut(s) 601
BciT130I CCWGG 1 cut(s) 450
BfaI CTAG 1 cut(s) 769
BfmI CTRYAG 2 cut(s) 77, 552
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 559
BisI GCNGC 2 cut(s) 482, 520
BlsI GCNGC 2 cut(s) 483, 521
Bme1390I CCNGG 1 cut(s) 450
Bme18I GGWCC 1 cut(s) 692
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 6 cut(s) 72, 73, 350, 601, 692, 714
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 2 cut(s) 223, 376
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 3 cut(s) 616, 705, 707
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 223, 376
BseRI GAGGAG 1 cut(s) 573
BseXI GCAGC 2 cut(s) 493, 531
BshFI GGCC 2 cut(s) 273, 330
BslI CCNNNNNNNGG 1 cut(s) 371
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 273, 330
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 2 cut(s) 308, 472
BspACI CCGC 1 cut(s) 695
BspANI GGCC 2 cut(s) 273, 330
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 214
BspMAI CTGCAG 2 cut(s) 81, 556
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 529
BsrGI TGTACA 1 cut(s) 595
BsrI ACTGG 2 cut(s) 223, 376
BssMI GATC 2 cut(s) 308, 472
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 6 cut(s) 132, 140, 244, 319, 737, 746
BstAPI GCANNNNNTGC 1 cut(s) 91
BstAUI TGTACA 1 cut(s) 595
BstDEI CTNAG 1 cut(s) 261
BstF5I GGATG 3 cut(s) 616, 705, 707
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 2 cut(s) 311, 475
BstMBI GATC 2 cut(s) 308, 472
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 448
BstSFI CTRYAG 2 cut(s) 77, 552
BstV1I GCAGC 2 cut(s) 493, 531
BsuRI GGCC 2 cut(s) 273, 330
BtsCI GGATG 3 cut(s) 616, 705, 707
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 5 cut(s) 74, 128, 216, 468, 742
BveI ACCTGC 1 cut(s) 559
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 692
CseI GACGC 1 cut(s) 655
Csp6I GTAC 2 cut(s) 387, 596
CviAII CATG 3 cut(s) 295, 390, 679
CviJI RGCY 6 cut(s) 215, 260, 273, 330, 378, 768
CviKI_1 RGCY 6 cut(s) 215, 260, 273, 330, 378, 768
CviQI GTAC 2 cut(s) 387, 596
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 310, 474
DpnII GATC 2 cut(s) 308, 472
Eco147I AGGCCT 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 692
Eco47III AGCGCT 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 448
EcoT22I ATGCAT 3 cut(s) 87, 343, 707
FaeI CATG 3 cut(s) 298, 393, 682
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 3 cut(s) 294, 389, 678
FauNDI CATATG 1 cut(s) 627
Fnu4HI GCNGC 2 cut(s) 482, 520
FokI GGATG 3 cut(s) 623, 692, 714
Fsp4HI GCNGC 2 cut(s) 482, 520
FspBI CTAG 1 cut(s) 769
GlaI GCGC 1 cut(s) 108
GluI GCNGC 2 cut(s) 482, 520
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 2 cut(s) 273, 330
HgaI GACGC 1 cut(s) 655
HhaI GCGC 1 cut(s) 109
Hin1II CATG 3 cut(s) 298, 393, 682
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 4 cut(s) 23, 125, 347, 663
HphI GGTGA 3 cut(s) 208, 445, 614
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 2 cut(s) 346, 442
Hpy188III TCNNGA 2 cut(s) 604, 667
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 6 cut(s) 132, 140, 244, 319, 737, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 3 cut(s) 298, 393, 682
HspAI GCGC 1 cut(s) 107
Kzo9I GATC 2 cut(s) 308, 472
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 9 cut(s) 117, 204, 357, 378, 422, 435, 462, 564, 652
Lsp1109I GCAGC 2 cut(s) 493, 531
LweI GCATC 6 cut(s) 72, 73, 350, 601, 692, 714
MaeI CTAG 1 cut(s) 769
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 310, 474
MboI GATC 2 cut(s) 308, 472
MboII GAAGA 2 cut(s) 59, 234
MfeI CAATTG 3 cut(s) 96, 615, 714
MluCI AATT 9 cut(s) 96, 151, 199, 334, 590, 615, 655, 687, 714
MlyI GAGTC 2 cut(s) 341, 672
MnlI CCTC 4 cut(s) 202, 294, 554, 594
Mph1103I ATGCAT 3 cut(s) 87, 343, 707
MseI TTAA 2 cut(s) 324, 395
MslI CAYNNNNRTG 1 cut(s) 759
MspR9I CCNGG 1 cut(s) 450
MunI CAATTG 3 cut(s) 96, 615, 714
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 1 cut(s) 91
NdeI CATATG 1 cut(s) 627
NdeII GATC 2 cut(s) 308, 472
NlaIII CATG 3 cut(s) 298, 393, 682
NlaIV GGNNCC 1 cut(s) 214
NsiI ATGCAT 3 cut(s) 87, 343, 707
NspI RCATGY 1 cut(s) 393
PceI AGGCCT 1 cut(s) 330
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 23, 125
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 2 cut(s) 483, 521
PleI GAGTC 2 cut(s) 341, 671
PpsI GAGTC 2 cut(s) 341, 671
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 2 cut(s) 81, 556
PstNI CAGNNNCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 388, 597
RsaNI GTAC 2 cut(s) 387, 596
RseI CAYNNNNRTG 1 cut(s) 759
SaqAI TTAA 2 cut(s) 324, 395
SatI GCNGC 2 cut(s) 482, 520
Sau3AI GATC 2 cut(s) 308, 472
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 2 cut(s) 341, 672
ScrFI CCNGG 1 cut(s) 450
SetI ASST 5 cut(s) 262, 435, 546, 553, 770
SfaNI GCATC 6 cut(s) 72, 73, 350, 601, 692, 714
SfcI CTRYAG 2 cut(s) 77, 552
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 759
Sse9I AATT 9 cut(s) 96, 151, 199, 334, 590, 615, 655, 687, 714
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 1 cut(s) 695
SspMI CTAG 1 cut(s) 769
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 6 cut(s) 132, 140, 244, 319, 737, 746
TaqI TCGA 3 cut(s) 207, 546, 561
TasI AATT 9 cut(s) 96, 151, 199, 334, 590, 615, 655, 687, 714
TatI WGTACW 1 cut(s) 595
TfiI GAWTC 2 cut(s) 23, 125
Tru1I TTAA 2 cut(s) 324, 395
Tru9I TTAA 2 cut(s) 324, 395
TscAI CASTG 5 cut(s) 81, 135, 223, 475, 742
TseI GCWGC 2 cut(s) 481, 519
TspDTI ATGAA 1 cut(s) 520
TspRI CASTG 5 cut(s) 81, 135, 223, 475, 742
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 393
XspI CTAG 1 cut(s) 769
Zsp2I ATGCAT 3 cut(s) 87, 343, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.