Rh2DG599000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013702
Physical Location & Seq
Reverse (-)
83120126 .. 83121028
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG599000.1

Sequence Viewer

Length: 636 bp
ATGATCGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACCTTCAACCTAAACTTTATAACACAAGCAAACGCTACCCTCCTCCACAGTTTGCACAACTTTTGATGGATGATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCATCCCATCGATTGCAAAGACACAAAACCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTCGCCTCGATTTTTAACGACAAACTTCGTGCAACTCTCGCAAATTTAACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCACAACTTGGAACAATGGGACATCGGCATGTATTCCATGGGCTAAACCATGCTTTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTTACTGAATCTGCTAGTTCAGTCGTAGCAACTGGCTGTTTCAATGATACAACTGTTTGCACTCCACTAAACATCAAGAAACTTGTACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

211

Amino Acids

23.32

Weight (kDa)

7.56

Isoelectric Point (pI)

20.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 8 - 190 3.2e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 96
AbsI CCTCGAGG 1 cut(s) 425
AciI CCGC 2 cut(s) 14, 272
AclWI GGATC 2 cut(s) 361, 392
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 2 cut(s) 20, 627
AgsI TTSAA 5 cut(s) 83, 209, 350, 506, 583
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 2 cut(s) 289, 377
AluI AGCT 2 cut(s) 289, 377
AlwI GGATC 2 cut(s) 361, 392
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 1 cut(s) 447
ApoI RAATTY 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BbvI GCAGC 1 cut(s) 434
BccI CCATC 2 cut(s) 136, 241
BciT130I CCWGG 1 cut(s) 190
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 48
BisI GCNGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 449
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 187, 217
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 2 cut(s) 237, 385
Bsa29I ATCGAT 2 cut(s) 6, 236
BsaJI CCNNGG 2 cut(s) 220, 485
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bse1I ACTGG 1 cut(s) 577
BseBI CCWGG 1 cut(s) 190
BseCI ATCGAT 2 cut(s) 6, 236
BseDI CCNNGG 2 cut(s) 220, 485
BseGI GGATG 2 cut(s) 151, 228
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 434
BsgI GTGCAG 1 cut(s) 433
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 1 cut(s) 423
BshVI ATCGAT 2 cut(s) 6, 236
BsiEI CGRYCG 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 425
BslFI GGGAC 4 cut(s) 201, 393, 481, 542
BsmFI GGGAC 4 cut(s) 201, 393, 481, 542
BsnI GGCC 1 cut(s) 423
BsoBI CYCGRG 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 5 cut(s) 3, 7, 148, 353, 384
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 2 cut(s) 14, 272
BspANI GGCC 1 cut(s) 423
BspDI ATCGAT 2 cut(s) 6, 236
BspLI GGNNCC 2 cut(s) 187, 217
BspPI GGATC 2 cut(s) 361, 392
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 2 cut(s) 220, 485
BssMI GATC 5 cut(s) 3, 7, 148, 353, 384
BssT1I CCWWGG 2 cut(s) 220, 485
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 126, 269, 595
BstAPI GCANNNNNTGC 1 cut(s) 444
BstAUI TGTACA 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 181
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 2 cut(s) 151, 228
BstKTI GATC 5 cut(s) 6, 10, 151, 356, 387
BstMBI GATC 5 cut(s) 3, 7, 148, 353, 384
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 2 cut(s) 326, 444
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
Bsu15I ATCGAT 2 cut(s) 6, 236
BsuRI GGCC 1 cut(s) 423
BsuTUI ATCGAT 2 cut(s) 6, 236
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 151, 228
Cac8I GCNNGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 215
ClaI ATCGAT 2 cut(s) 6, 236
CseI GACGC 1 cut(s) 539
Csp6I GTAC 2 cut(s) 19, 626
CviAII CATG 4 cut(s) 444, 477, 486, 498
CviJI RGCY 7 cut(s) 188, 289, 377, 390, 423, 491, 576
CviKI_1 RGCY 7 cut(s) 188, 289, 377, 390, 423, 491, 576
CviQI GTAC 2 cut(s) 19, 626
DdeI CTNAG 1 cut(s) 181
DpnI GATC 5 cut(s) 5, 9, 150, 355, 386
DpnII GATC 5 cut(s) 3, 7, 148, 353, 384
Eco130I CCWWGG 2 cut(s) 220, 485
Eco47I GGWCC 1 cut(s) 215
Eco88I CYCGRG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 2 cut(s) 220, 485
ErhI CCWWGG 2 cut(s) 220, 485
FaeI CATG 4 cut(s) 447, 480, 489, 501
FaqI GGGAC 4 cut(s) 201, 393, 481, 542
FatI CATG 4 cut(s) 443, 476, 485, 497
Fnu4HI GCNGC 1 cut(s) 448
FokI GGATG 2 cut(s) 158, 215
Fsp4HI GCNGC 1 cut(s) 448
FspBI CTAG 1 cut(s) 555
GluI GCNGC 1 cut(s) 448
HaeIII GGCC 1 cut(s) 423
HgaI GACGC 1 cut(s) 539
Hin1II CATG 4 cut(s) 447, 480, 489, 501
HinfI GANTC 1 cut(s) 548
HphI GGTGA 1 cut(s) 149
Hpy188III TCNNGA 1 cut(s) 616
HpyAV CCTTC 2 cut(s) 89, 269
HpyCH4III ACNGT 3 cut(s) 126, 269, 595
HpyCH4V TGCA 7 cut(s) 131, 228, 242, 320, 398, 450, 600
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 444
HpyF3I CTNAG 1 cut(s) 181
Hsp92II CATG 4 cut(s) 447, 480, 489, 501
Kzo9I GATC 5 cut(s) 3, 7, 148, 353, 384
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 4 cut(s) 175, 202, 511, 558
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 2 cut(s) 237, 385
MaeI CTAG 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 515
MalI GATC 5 cut(s) 5, 9, 150, 355, 386
MboI GATC 5 cut(s) 3, 7, 148, 353, 384
MboII GAAGA 1 cut(s) 514
MflI RGATCY 1 cut(s) 353
MluCI AATT 4 cut(s) 73, 210, 331, 399
MnlI CCTC 6 cut(s) 126, 129, 304, 421, 422, 434
MseI TTAA 3 cut(s) 69, 303, 335
MslI CAYNNNNRTG 1 cut(s) 475
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 326, 444
NcoI CCATGG 1 cut(s) 485
NdeII GATC 5 cut(s) 3, 7, 148, 353, 384
NlaIII CATG 4 cut(s) 447, 480, 489, 501
NlaIV GGNNCC 2 cut(s) 187, 217
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 1 cut(s) 480
PaeR7I CTCGAG 1 cut(s) 425
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 1 cut(s) 449
PsiI TTATAA 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 187, 217
PspPI GGNCC 1 cut(s) 215
PspXI VCTCGAGB 1 cut(s) 425
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 2 cut(s) 20, 627
RsaNI GTAC 2 cut(s) 19, 626
RseI CAYNNNNRTG 1 cut(s) 475
SaqAI TTAA 3 cut(s) 69, 303, 335
SatI GCNGC 1 cut(s) 448
Sau3AI GATC 5 cut(s) 3, 7, 148, 353, 384
Sau96I GGNCC 1 cut(s) 215
ScaI AGTACT 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 190
SetI ASST 4 cut(s) 81, 88, 291, 379
SfaNI GCATC 2 cut(s) 237, 385
SfcI CTRYAG 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 425
SinI GGWCC 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 475
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 4 cut(s) 73, 210, 331, 399
SsiI CCGC 2 cut(s) 14, 272
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 2 cut(s) 220, 485
TaaI ACNGT 3 cut(s) 126, 269, 595
TaqI TCGA 5 cut(s) 6, 236, 296, 373, 426
TaqII GACCGA 1 cut(s) 359
TasI AATT 4 cut(s) 73, 210, 331, 399
TatI WGTACW 2 cut(s) 18, 625
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 3 cut(s) 69, 303, 335
Tru9I TTAA 3 cut(s) 69, 303, 335
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 1 cut(s) 447
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 480
XhoI CTCGAG 1 cut(s) 425
XspI CTAG 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.