Rroxscaffold_2G00086440

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013702
Physical Location & Seq
Forward (+)
8632310 .. 8634073
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086440.1

Sequence Viewer

Length: 933 bp
ATGAAAAACATGGGAATAAAACTATCAATTTCATCACTTATTTTGCTGTTCATGAGTTTTGCGAATTTTAGCCCAGTTTTAGTGGAACGCTCGCCGATTGCACCAGCATTGTACGTGTTTGGGGATTCTTTGTTTGACAGGGGTAACAATAATTTCTTGCCAACTATAGCAAAGTCAAATTTCCTGCCCTATGGTGTCAATTTTGTGGAGGGTGTCACCGGAAGATTCACCAACGGTAGAACAGTAGCGGATTTTATAGCTGAGTTTCTTGGGCTACCATCCGCTCCACCATACTTGAGCATATATGAATCAACAATACTTACCGGCCTGAATTATGCATCTGGGTCTTGTGGCATTCTCCCGGATACTGGAAGCCGATTGGGGAAGTGCTTGAATTTGAAAGAGCAGATGGATTTGTTTGAAAAAATGGTGAAATCAGACTTCTCAGGACATTTCAAAAATCCAAATGATCTTGCAAAGTACTTGTCCAAGTCCATATTTTTATTTTCTGTAGGCAGCAATGATTATCTTAATAATTACCTTCAGCCTAAACTTTATAACACAAGCAAACGCTACCCTCCTCCAAAGTTTGCACAACTCTTGATGGATGCTCTTTCTCACCAGTTTGAGAGGTTATTTAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCATTGGTTGCATCCCAGCAATTGCAATCCACTTCGCCACCTTCGTTCTTCCTCCTCCGGTCACCGTTTGCTTCGATTCCAAAGGGAATCCTGCTCTCCTCAGTCCCCTCTACACGTCTGTGGTGGTGATTCGTTGTGGGAAGCACCGTAGACGACGCTACAAGGCCGAGAAGTTGCAGGGTAGAGCTGAAAATCGCCCAAATTCGCCGACCTTGGTTCTCCCAACTCCGGCCACCATAGCTCGAGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

310

Amino Acids

34.36

Weight (kDa)

9.62

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 237 6.5e-17 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 829
AciI CCGC 2 cut(s) 248, 282
AcoI YGGCCR 1 cut(s) 909
AcsI RAATTY 4 cut(s) 64, 178, 394, 880
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 2 cut(s) 113, 482
AfiI CCNNNNNNNGG 2 cut(s) 368, 907
AflIII ACRYGT 2 cut(s) 114, 792
AgsI TTSAA 5 cut(s) 394, 400, 422, 457, 671
AjiI CACGTC 1 cut(s) 795
AjnI CCWGG 1 cut(s) 650
AleI CACNNNNGTG 1 cut(s) 797
AluBI AGCT 3 cut(s) 260, 866, 920
AluI AGCT 3 cut(s) 260, 866, 920
Ama87I CYCGRG 1 cut(s) 921
AoxI GGCC 3 cut(s) 325, 843, 909
ApeKI GCWGC 1 cut(s) 516
ApoI RAATTY 4 cut(s) 64, 178, 394, 880
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspS9I GGNCC 1 cut(s) 677
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 6 cut(s) 208, 220, 442, 611, 733, 817
AvaI CYCGRG 1 cut(s) 921
AvaII GGWCC 1 cut(s) 677
BbvI GCAGC 1 cut(s) 528
BccI CCATC 3 cut(s) 286, 403, 598
BciT130I CCWGG 1 cut(s) 652
BciVI GTATCC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 362
BfmI CTRYAG 2 cut(s) 165, 510
BfuI GTATCC 1 cut(s) 358
BisI GCNGC 1 cut(s) 517
BlsI GCNGC 1 cut(s) 518
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 2 cut(s) 362, 652
Bme18I GGWCC 1 cut(s) 677
BmeT110I CYCGRG 1 cut(s) 921
BmgBI CACGTC 1 cut(s) 795
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 2 cut(s) 649, 679
BmrFI CCNGG 2 cut(s) 362, 652
BmrI ACTGGG 1 cut(s) 68
BmsI GCATC 3 cut(s) 347, 598, 699
BmuI ACTGGG 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 316
BpuMI CCSGG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 891
BsaWI WCCGGW 2 cut(s) 218, 736
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 907
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 3 cut(s) 74, 373, 622
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 891
BseGI GGATG 3 cut(s) 278, 613, 690
BseLI CCNNNNNNNGG 2 cut(s) 368, 907
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 3 cut(s) 252, 459, 793
BseNI ACTGG 3 cut(s) 74, 373, 622
BseRI GAGGAG 3 cut(s) 570, 723, 767
BseXI GCAGC 1 cut(s) 528
BseYI CCCAGC 1 cut(s) 694
BshFI GGCC 3 cut(s) 327, 845, 911
BsiHKCI CYCGRG 1 cut(s) 921
BsiSI CCGG 5 cut(s) 219, 324, 362, 737, 908
BslFI GGGAC 2 cut(s) 663, 768
BslI CCNNNNNNNGG 2 cut(s) 368, 907
BsmFI GGGAC 2 cut(s) 663, 768
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 3 cut(s) 327, 845, 911
BsoBI CYCGRG 1 cut(s) 921
Bsp143I GATC 1 cut(s) 469
BspACI CCGC 2 cut(s) 248, 282
BspANI GGCC 3 cut(s) 327, 845, 911
BspCNI CTCAG 3 cut(s) 253, 458, 792
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 649, 679
BsrBI CCGCTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 526
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 3 cut(s) 74, 373, 622
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 891
BssMI GATC 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 4 cut(s) 236, 244, 745, 827
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 4 cut(s) 261, 445, 643, 779
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 3 cut(s) 278, 613, 690
BstKTI GATC 1 cut(s) 472
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 1 cut(s) 917
BstNI CCWGG 1 cut(s) 652
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 360, 650
BstSFI CTRYAG 2 cut(s) 165, 510
BstV1I GCAGC 1 cut(s) 528
BsuI GTATCC 1 cut(s) 358
BsuRI GGCC 3 cut(s) 327, 845, 911
BtrI CACGTC 1 cut(s) 795
BtsCI GGATG 3 cut(s) 278, 613, 690
Cac8I GCNNGC 1 cut(s) 92
CciI TCATGA 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 677
CseI GACGC 1 cut(s) 843
Csp6I GTAC 2 cut(s) 112, 481
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 2 cut(s) 10, 52
CviQI GTAC 2 cut(s) 112, 481
DdeI CTNAG 4 cut(s) 261, 445, 643, 779
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 909
Eco130I CCWWGG 1 cut(s) 891
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 921
Eco91I GGTNACC 1 cut(s) 739
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 650
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 2 cut(s) 13, 55
FaqI GGGAC 2 cut(s) 663, 768
FatI CATG 2 cut(s) 9, 51
FblI GTMKAC 1 cut(s) 829
Fnu4HI GCNGC 1 cut(s) 517
FokI GGATG 3 cut(s) 265, 620, 677
Fsp4HI GCNGC 1 cut(s) 517
GluI GCNGC 1 cut(s) 517
GsaI CCCAGC 1 cut(s) 698
HaeIII GGCC 3 cut(s) 327, 845, 911
HapII CCGG 5 cut(s) 219, 324, 362, 737, 908
HgaI GACGC 1 cut(s) 843
Hin1II CATG 2 cut(s) 13, 55
HinfI GANTC 6 cut(s) 125, 225, 308, 755, 766, 808
HpaII CCGG 5 cut(s) 219, 324, 362, 737, 908
HphI GGTGA 6 cut(s) 208, 220, 442, 611, 733, 817
Hpy166II GTNNAC 1 cut(s) 830
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 3 cut(s) 52, 447, 601
Hpy8I GTNNAC 1 cut(s) 830
Hpy99I CGWCG 1 cut(s) 837
HpyAV CCTTC 2 cut(s) 551, 730
HpyCH4III ACNGT 4 cut(s) 236, 244, 745, 827
HpyCH4IV ACGT 2 cut(s) 114, 794
HpyCH4V TGCA 7 cut(s) 101, 338, 476, 593, 690, 704, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 917
HpyF3I CTNAG 4 cut(s) 261, 445, 643, 779
HpySE526I ACGT 2 cut(s) 114, 794
Hsp92II CATG 2 cut(s) 13, 55
Kzo9I GATC 1 cut(s) 469
LmnI GCTCC 2 cut(s) 289, 647
Lsp1109I GCAGC 1 cut(s) 528
LweI GCATC 3 cut(s) 347, 598, 699
MaeII ACGT 2 cut(s) 114, 794
MaeIII GTNAC 3 cut(s) 143, 214, 739
MalI GATC 1 cut(s) 471
MbiI CCGCTC 1 cut(s) 284
MboI GATC 1 cut(s) 469
MboII GAAGA 2 cut(s) 234, 719
MfeI CAATTG 1 cut(s) 699
MnlI CCTC 8 cut(s) 202, 588, 591, 624, 741, 744, 788, 797
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 531, 639
MslI CAYNNNNRTG 1 cut(s) 797
MspI CCGG 5 cut(s) 219, 324, 362, 737, 908
MspR9I CCNGG 2 cut(s) 362, 652
MunI CAATTG 1 cut(s) 699
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 652
MwoI GCNNNNNNNGC 1 cut(s) 917
NciI CCSGG 1 cut(s) 362
NdeII GATC 1 cut(s) 469
NlaIII CATG 2 cut(s) 13, 55
NlaIV GGNNCC 2 cut(s) 649, 679
NmeAIII GCCGAG 1 cut(s) 871
NmuCI GTSAC 2 cut(s) 214, 739
NsiI ATGCAT 1 cut(s) 340
OliI CACNNNNGTG 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 921
PagI TCATGA 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 6 cut(s) 125, 225, 308, 755, 766, 808
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 115
PsiI TTATAA 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 650
PspEI GGTNACC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 2 cut(s) 649, 679
PspPI GGNCC 1 cut(s) 677
PspXI VCTCGAGB 1 cut(s) 921
RsaI GTAC 2 cut(s) 113, 482
RsaNI GTAC 2 cut(s) 112, 481
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 2 cut(s) 531, 639
SatI GCNGC 1 cut(s) 517
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 1 cut(s) 677
ScaI AGTACT 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 362, 652
SetI ASST 9 cut(s) 117, 262, 543, 635, 722, 797, 868, 893, 922
SfaNI GCATC 3 cut(s) 347, 598, 699
SfcI CTRYAG 2 cut(s) 165, 510
Sfr274I CTCGAG 1 cut(s) 921
SinI GGWCC 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 921
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 2 cut(s) 295, 921
SmoI CTYRAG 2 cut(s) 295, 921
SsiI CCGC 2 cut(s) 248, 282
StyD4I CCNGG 2 cut(s) 360, 650
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 4 cut(s) 236, 244, 745, 827
TaiI ACGT 2 cut(s) 117, 797
TaqI TCGA 2 cut(s) 753, 922
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 6 cut(s) 125, 225, 308, 755, 766, 808
Tru1I TTAA 2 cut(s) 531, 639
Tru9I TTAA 2 cut(s) 531, 639
TseFI GTSAC 2 cut(s) 214, 739
TseI GCWGC 1 cut(s) 516
Tsp45I GTSAC 2 cut(s) 214, 739
TspDTI ATGAA 4 cut(s) 17, 21, 40, 321
VpaK11BI GGWCC 1 cut(s) 677
XapI RAATTY 4 cut(s) 64, 178, 394, 880
XhoI CTCGAG 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 829
ZrmI AGTACT 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.