Rw2G047930

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013702
Physical Location & Seq
Reverse (-)
75210689 .. 75212383
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047930.1

Sequence Viewer

Length: 1089 bp
ATGGGAATCAAACTATCAATTTCATCACTTATTTTTCTGTTCTTGAGTTTTGCAAATTTTGGCTCAGTTTTAATTGATGGATCGCCGATTGCACCAGCATTATACGTGTTTGGGGATTCTTTGTTTGATAGTGGTAACAATAATTTCTTGCCAACTTTAGCAAAGGCAGATTTCCTGCCCTATGGCGTCAATTTTGCGAAGGGTGTCACCGGAAGATTTACCAACGGTAAAACAGTAGCAGATTTTATAGCTGAGTTTCTTGGGCTACCATACGCTCCACCATGCTTGAGCATACACAAATCGACAATACTTACCGGCCTGAATTATGCATCTGGGTCTTGTGGCATCCTCCCGGAAACCGGAAGCCAATTGGGGAAGTGCTTGAATCTGAAAGAGCAGATGGATTTGTTTGAAAAAACGGTGAGGTCAGACTTGTCACGACATTTCAAAAATCCCAATGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACCTTCAACCTAAACTCTATAACACAAGCAAACGCTACCCTTCTCCACAGTTTGCACAACTCTTGATGGATCATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCACCCCATCCATTGCAAAGACACAAAGCCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTCGCCTCGATTTTTAACGACAAACTTCGTGCAACTCTCGCAAATTTAACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCACAACTTGGAACAATGGGACATCGGCATGTATTCCATGGGCTAAACCATGCTTTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTGACTGAATCTGCTAGTTCAGTCATAGCAACTGGCTGTTTCAATGATACAACTGTTTGCACTCCACTAAACATCAAGAAACTTGTACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.5

Weight (kDa)

8.23

Isoelectric Point (pI)

28.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 341 3.5e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 878
AciI CCGC 2 cut(s) 467, 725
AclWI GGATC 4 cut(s) 88, 606, 814, 845
AcsI RAATTY 2 cut(s) 55, 784
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 473, 1080
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 8 cut(s) 385, 413, 448, 536, 662, 803, 959, 1036
AjnI CCWGG 1 cut(s) 641
AluBI AGCT 3 cut(s) 251, 742, 830
AluI AGCT 3 cut(s) 251, 742, 830
AlwI GGATC 4 cut(s) 88, 606, 814, 845
Ama87I CYCGRG 1 cut(s) 878
AoxI GGCC 2 cut(s) 316, 874
ApeKI GCWGC 1 cut(s) 900
ApoI RAATTY 2 cut(s) 55, 784
AspS9I GGNCC 1 cut(s) 668
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 199, 433, 602
AvaI CYCGRG 1 cut(s) 878
AvaII GGWCC 1 cut(s) 668
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BbvI GCAGC 1 cut(s) 887
BccI CCATC 4 cut(s) 71, 394, 589, 694
BciT130I CCWGG 1 cut(s) 643
BcnI CCSGG 1 cut(s) 353
BfaI CTAG 1 cut(s) 1008
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 1 cut(s) 901
BlsI GCNGC 1 cut(s) 902
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 2 cut(s) 353, 643
Bme18I GGWCC 1 cut(s) 668
BmeT110I CYCGRG 1 cut(s) 878
BmgT120I GGNCC 1 cut(s) 668
BmiI GGNNCC 2 cut(s) 640, 670
BmrFI CCNGG 2 cut(s) 353, 643
BmsI GCATC 3 cut(s) 338, 354, 838
BpuEI CTTGAG 2 cut(s) 64, 307
BpuMI CCSGG 1 cut(s) 353
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 673, 938
BsaWI WCCGGW 2 cut(s) 209, 359
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 1030
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 1 cut(s) 643
BseDI CCNNGG 2 cut(s) 673, 938
BseGI GGATG 2 cut(s) 345, 686
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 2 cut(s) 78, 243
BseNI ACTGG 1 cut(s) 1030
BseXI GCAGC 1 cut(s) 887
BsgI GTGCAG 1 cut(s) 886
Bsh1285I CGRYCG 1 cut(s) 826
BshFI GGCC 2 cut(s) 318, 876
BsiEI CGRYCG 1 cut(s) 826
BsiHKCI CYCGRG 1 cut(s) 878
BsiSI CCGG 4 cut(s) 210, 315, 353, 360
BslFI GGGAC 4 cut(s) 654, 846, 934, 995
BslI CCNNNNNNNGG 1 cut(s) 359
BsmFI GGGAC 4 cut(s) 654, 846, 934, 995
BsnI GGCC 2 cut(s) 318, 876
BsoBI CYCGRG 1 cut(s) 878
Bsp1407I TGTACA 1 cut(s) 1078
Bsp143I GATC 5 cut(s) 80, 460, 598, 806, 837
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 2 cut(s) 467, 725
BspANI GGCC 2 cut(s) 318, 876
BspCNI CTCAG 2 cut(s) 77, 244
BspLI GGNNCC 2 cut(s) 640, 670
BspPI GGATC 4 cut(s) 88, 606, 814, 845
BsrDI GCAATG 1 cut(s) 690
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 1078
BsrI ACTGG 1 cut(s) 1030
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 673, 938
BssMI GATC 5 cut(s) 80, 460, 598, 806, 837
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 673, 938
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 6 cut(s) 227, 235, 421, 579, 722, 1048
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 897
BstAUI TGTACA 1 cut(s) 1078
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 744
BstDEI CTNAG 3 cut(s) 64, 252, 634
BstDSI CCRYGG 1 cut(s) 938
BstF5I GGATG 2 cut(s) 345, 686
BstKTI GATC 5 cut(s) 83, 463, 601, 809, 840
BstMBI GATC 5 cut(s) 80, 460, 598, 806, 837
BstMCI CGRYCG 1 cut(s) 826
BstMWI GCNNNNNNNGC 2 cut(s) 779, 897
BstNI CCWGG 1 cut(s) 643
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 2 cut(s) 351, 641
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 1 cut(s) 887
BstX2I RGATCY 1 cut(s) 806
BstYI RGATCY 1 cut(s) 806
BsuRI GGCC 2 cut(s) 318, 876
BtgI CCRYGG 1 cut(s) 938
BtsCI GGATG 2 cut(s) 345, 686
Cac8I GCNNGC 1 cut(s) 744
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 668
CseI GACGC 2 cut(s) 175, 992
Csp6I GTAC 2 cut(s) 472, 1079
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 5 cut(s) 282, 897, 930, 939, 951
CviQI GTAC 2 cut(s) 472, 1079
DdeI CTNAG 3 cut(s) 64, 252, 634
DpnI GATC 5 cut(s) 82, 462, 600, 808, 839
DpnII GATC 5 cut(s) 80, 460, 598, 806, 837
Eco130I CCWWGG 2 cut(s) 673, 938
Eco47I GGWCC 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 878
EcoRII CCWGG 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 673, 938
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 673, 938
FaeI CATG 5 cut(s) 285, 900, 933, 942, 954
FaqI GGGAC 4 cut(s) 654, 846, 934, 995
FatI CATG 5 cut(s) 281, 896, 929, 938, 950
Fnu4HI GCNGC 1 cut(s) 901
FokI GGATG 2 cut(s) 332, 673
Fsp4HI GCNGC 1 cut(s) 901
FspBI CTAG 1 cut(s) 1008
GluI GCNGC 1 cut(s) 901
HaeIII GGCC 2 cut(s) 318, 876
HapII CCGG 4 cut(s) 210, 315, 353, 360
HgaI GACGC 2 cut(s) 175, 992
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 5 cut(s) 285, 900, 933, 942, 954
HinfI GANTC 4 cut(s) 6, 116, 385, 1001
HpaII CCGG 4 cut(s) 210, 315, 353, 360
HphI GGTGA 3 cut(s) 199, 433, 602
Hpy188I TCNGA 3 cut(s) 390, 430, 996
Hpy188III TCNNGA 4 cut(s) 43, 438, 592, 1069
HpyAV CCTTC 4 cut(s) 193, 542, 579, 722
HpyCH4III ACNGT 6 cut(s) 227, 235, 421, 579, 722, 1048
HpyCH4IV ACGT 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 2 cut(s) 779, 897
HpyF3I CTNAG 3 cut(s) 64, 252, 634
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 5 cut(s) 285, 900, 933, 942, 954
Kzo9I GATC 5 cut(s) 80, 460, 598, 806, 837
LmnI GCTCC 2 cut(s) 280, 638
Lsp1109I GCAGC 1 cut(s) 887
LweI GCATC 3 cut(s) 338, 354, 838
MaeI CTAG 1 cut(s) 1008
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 134, 205, 435, 968
MalI GATC 5 cut(s) 82, 462, 600, 808, 839
MboI GATC 5 cut(s) 80, 460, 598, 806, 837
MboII GAAGA 2 cut(s) 225, 967
MfeI CAATTG 1 cut(s) 368
MflI RGATCY 1 cut(s) 806
MnlI CCTC 6 cut(s) 359, 417, 757, 874, 875, 887
Mph1103I ATGCAT 1 cut(s) 331
MseI TTAA 4 cut(s) 71, 522, 756, 788
MslI CAYNNNNRTG 1 cut(s) 928
MspI CCGG 4 cut(s) 210, 315, 353, 360
MspR9I CCNGG 2 cut(s) 353, 643
MunI CAATTG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 643
MwoI GCNNNNNNNGC 2 cut(s) 779, 897
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 1 cut(s) 938
NdeII GATC 5 cut(s) 80, 460, 598, 806, 837
NlaIII CATG 5 cut(s) 285, 900, 933, 942, 954
NlaIV GGNNCC 2 cut(s) 640, 670
NmuCI GTSAC 3 cut(s) 205, 435, 968
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 933
PaeR7I CTCGAG 1 cut(s) 878
PfeI GAWTC 4 cut(s) 6, 116, 385, 1001
PfoI TCCNGGA 1 cut(s) 351
PkrI GCNGC 1 cut(s) 902
Ppu21I YACGTR 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 641
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 2 cut(s) 640, 670
PspPI GGNCC 1 cut(s) 668
PspXI VCTCGAGB 1 cut(s) 878
PsuI RGATCY 1 cut(s) 806
RsaI GTAC 2 cut(s) 473, 1080
RsaNI GTAC 2 cut(s) 472, 1079
RseI CAYNNNNRTG 1 cut(s) 928
SaqAI TTAA 4 cut(s) 71, 522, 756, 788
SatI GCNGC 1 cut(s) 901
Sau3AI GATC 5 cut(s) 80, 460, 598, 806, 837
Sau96I GGNCC 1 cut(s) 668
ScaI AGTACT 1 cut(s) 473
ScrFI CCNGG 2 cut(s) 353, 643
SetI ASST 7 cut(s) 108, 253, 428, 534, 541, 744, 832
SfaNI GCATC 3 cut(s) 338, 354, 838
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 878
SinI GGWCC 1 cut(s) 668
SlaI CTCGAG 1 cut(s) 878
SmiMI CAYNNNNRTG 1 cut(s) 928
SmlI CTYRAG 3 cut(s) 43, 286, 878
SmoI CTYRAG 3 cut(s) 43, 286, 878
SsiI CCGC 2 cut(s) 467, 725
SspMI CTAG 1 cut(s) 1008
StyD4I CCNGG 2 cut(s) 351, 641
StyI CCWWGG 2 cut(s) 673, 938
TaaI ACNGT 6 cut(s) 227, 235, 421, 579, 722, 1048
TaiI ACGT 1 cut(s) 108
TaqI TCGA 4 cut(s) 302, 749, 826, 879
TaqII GACCGA 1 cut(s) 812
TatI WGTACW 2 cut(s) 471, 1078
TfiI GAWTC 4 cut(s) 6, 116, 385, 1001
Tru1I TTAA 4 cut(s) 71, 522, 756, 788
Tru9I TTAA 4 cut(s) 71, 522, 756, 788
TseFI GTSAC 3 cut(s) 205, 435, 968
TseI GCWGC 1 cut(s) 900
Tsp45I GTSAC 3 cut(s) 205, 435, 968
TspDTI ATGAA 2 cut(s) 12, 980
TspGWI ACGGA 1 cut(s) 900
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 2 cut(s) 55, 784
XceI RCATGY 1 cut(s) 933
XhoI CTCGAG 1 cut(s) 878
XspI CTAG 1 cut(s) 1008
ZrmI AGTACT 1 cut(s) 473
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.