Rh2AG577200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013702
Physical Location & Seq
Reverse (-)
80698061 .. 80711667
13607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG577200.1

Sequence Viewer

Length: 840 bp
ATGGATTTGTTTGAAAAAACGGTGAGGTCAGACTTGTCACGACATTTCAAAAATCCCAATGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACATTCAACCTAAACTCTATAACACAAGCAAACGCTACCCTCCTCCACAGTTTGCACAACTCTTGATGGATAATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCACCCCATCAATTGCAAAGACACAAAGCCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTCGCTTCGATTTTTAACGACAAACTTCGTGCAACACTCGCAAATTTAACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCACAACTTGGAAGAATGGAACATCGGCATGTATTCCATGGGCTAAACCATGCTTTCAACCAAATAGTCACTTCTTCTGGGACGCTTTTCATCTGACTGAATCTGCTAGTTCAGTCATAGCAACTGGCTGTTTCAATGATACAACTGGGAAGTGCTTGAATTTGAAAGAGCAAATGGATTTGTTTGAAAAAACGGTGAAGTCAGACTCCTCAGGACATTTCAAAAATCCAAATGATCTTGAAGAGCATTTAGGACAGAAGCAATCCATGCTGCACAGGTTGGTTACGGGGAACATCGGCCTGTACTCCATTGGCTATACCATGCTCTCATCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

279

Amino Acids

31.12

Weight (kDa)

8.35

Isoelectric Point (pI)

24.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 24 - 208 1.1e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 479
AciI CCGC 2 cut(s) 68, 326
AclWI GGATC 2 cut(s) 415, 446
AcsI RAATTY 2 cut(s) 385, 661
AfaI GTAC 2 cut(s) 74, 806
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 2 cut(s) 343, 431
AluI AGCT 2 cut(s) 343, 431
AlwI GGATC 2 cut(s) 415, 446
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 475, 799
ApeKI GCWGC 2 cut(s) 501, 772
ApoI RAATTY 2 cut(s) 385, 661
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 3 cut(s) 34, 203, 709
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 269
AxyI CCTNAGG 1 cut(s) 712
BbvI GCAGC 2 cut(s) 488, 759
BccI CCATC 2 cut(s) 190, 295
BciT130I CCWGG 1 cut(s) 244
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 102
BisI GCNGC 2 cut(s) 502, 773
BlsI GCNGC 2 cut(s) 503, 774
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 2 cut(s) 241, 271
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 1 cut(s) 439
BmuI ACTGGG 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 274, 539
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bse1I ACTGG 2 cut(s) 631, 652
Bse21I CCTNAGG 1 cut(s) 712
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 274, 539
BseGI GGATG 1 cut(s) 830
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 2 cut(s) 631, 652
BseRI GAGGAG 2 cut(s) 162, 700
BseXI GCAGC 2 cut(s) 488, 759
BsgI GTGCAG 2 cut(s) 487, 758
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 477, 801
BsiEI CGRYCG 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 479
BslFI GGGAC 3 cut(s) 255, 447, 596
BsmFI GGGAC 3 cut(s) 255, 447, 596
BsnI GGCC 2 cut(s) 477, 801
BsoBI CYCGRG 1 cut(s) 479
Bsp143I GATC 4 cut(s) 61, 407, 438, 736
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 2 cut(s) 68, 326
BspANI GGCC 2 cut(s) 477, 801
BspCNI CTCAG 1 cut(s) 725
BspLI GGNNCC 2 cut(s) 241, 271
BspPI GGATC 2 cut(s) 415, 446
BspQI GCTCTTC 1 cut(s) 738
BsrI ACTGG 2 cut(s) 631, 652
BssECI CCNNGG 2 cut(s) 274, 539
BssMI GATC 4 cut(s) 61, 407, 438, 736
BssT1I CCWWGG 2 cut(s) 274, 539
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 4 cut(s) 22, 180, 323, 697
Bst6I CTCTTC 1 cut(s) 738
BstAPI GCANNNNNTGC 2 cut(s) 498, 769
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 2 cut(s) 235, 712
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 1 cut(s) 830
BstKTI GATC 4 cut(s) 64, 410, 441, 739
BstMBI GATC 4 cut(s) 61, 407, 438, 736
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 3 cut(s) 380, 498, 769
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 488, 759
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
Bsu36I CCTNAGG 1 cut(s) 712
BsuRI GGCC 2 cut(s) 477, 801
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 1 cut(s) 830
Cac8I GCNNGC 1 cut(s) 345
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 73, 805
CviAII CATG 6 cut(s) 498, 531, 540, 552, 769, 823
CviQI GTAC 2 cut(s) 73, 805
DdeI CTNAG 2 cut(s) 235, 712
DpnI GATC 4 cut(s) 63, 409, 440, 738
DpnII GATC 4 cut(s) 61, 407, 438, 736
Eam1104I CTCTTC 1 cut(s) 738
EarI CTCTTC 1 cut(s) 738
Eco130I CCWWGG 2 cut(s) 274, 539
Eco47I GGWCC 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 712
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 274, 539
ErhI CCWWGG 2 cut(s) 274, 539
FaeI CATG 6 cut(s) 501, 534, 543, 555, 772, 826
FaqI GGGAC 3 cut(s) 255, 447, 596
FatI CATG 6 cut(s) 497, 530, 539, 551, 768, 822
Fnu4HI GCNGC 2 cut(s) 502, 773
FokI GGATG 1 cut(s) 817
Fsp4HI GCNGC 2 cut(s) 502, 773
FspBI CTAG 1 cut(s) 609
GluI GCNGC 2 cut(s) 502, 773
HaeIII GGCC 2 cut(s) 477, 801
HgaI GACGC 1 cut(s) 593
Hin1II CATG 6 cut(s) 501, 534, 543, 555, 772, 826
HinfI GANTC 2 cut(s) 602, 707
HphI GGTGA 3 cut(s) 34, 203, 709
Hpy188I TCNGA 3 cut(s) 31, 597, 706
Hpy188III TCNNGA 4 cut(s) 39, 193, 714, 740
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 4 cut(s) 22, 180, 323, 697
HpyCH4V TGCA 7 cut(s) 185, 282, 296, 374, 452, 504, 775
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 498, 769
HpyF3I CTNAG 2 cut(s) 235, 712
Hsp92II CATG 6 cut(s) 501, 534, 543, 555, 772, 826
Kzo9I GATC 4 cut(s) 61, 407, 438, 736
LguI GCTCTTC 1 cut(s) 738
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 8 cut(s) 229, 256, 565, 612, 633, 699, 763, 815
Lsp1109I GCAGC 2 cut(s) 488, 759
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 3 cut(s) 36, 569, 784
MalI GATC 4 cut(s) 63, 409, 440, 738
MboI GATC 4 cut(s) 61, 407, 438, 736
MboII GAAGA 3 cut(s) 526, 568, 755
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 407
MluCI AATT 6 cut(s) 127, 264, 291, 385, 453, 661
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 7 cut(s) 18, 180, 183, 475, 476, 488, 721
MseI TTAA 3 cut(s) 123, 357, 389
MslI CAYNNNNRTG 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 244
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 3 cut(s) 380, 498, 769
NcoI CCATGG 1 cut(s) 539
NdeII GATC 4 cut(s) 61, 407, 438, 736
NlaIII CATG 6 cut(s) 501, 534, 543, 555, 772, 826
NlaIV GGNNCC 2 cut(s) 241, 271
NmuCI GTSAC 2 cut(s) 36, 569
NspI RCATGY 1 cut(s) 534
PaeR7I CTCGAG 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 602
PkrI GCNGC 2 cut(s) 503, 774
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 241, 271
PspPI GGNCC 1 cut(s) 269
PspXI VCTCGAGB 1 cut(s) 479
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 2 cut(s) 74, 806
RsaNI GTAC 2 cut(s) 73, 805
RseI CAYNNNNRTG 1 cut(s) 529
SapI GCTCTTC 1 cut(s) 738
SaqAI TTAA 3 cut(s) 123, 357, 389
SatI GCNGC 2 cut(s) 502, 773
Sau3AI GATC 4 cut(s) 61, 407, 438, 736
Sau96I GGNCC 1 cut(s) 269
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 1 cut(s) 244
SetI ASST 5 cut(s) 29, 142, 345, 433, 782
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 6 cut(s) 127, 264, 291, 385, 453, 661
SsiI CCGC 2 cut(s) 68, 326
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 2 cut(s) 274, 539
TaaI ACNGT 4 cut(s) 22, 180, 323, 697
TaqI TCGA 3 cut(s) 350, 427, 480
TaqII GACCGA 1 cut(s) 413
TasI AATT 6 cut(s) 127, 264, 291, 385, 453, 661
TatI WGTACW 2 cut(s) 72, 804
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 3 cut(s) 123, 357, 389
Tru9I TTAA 3 cut(s) 123, 357, 389
TseFI GTSAC 2 cut(s) 36, 569
TseI GCWGC 2 cut(s) 501, 772
Tsp45I GTSAC 2 cut(s) 36, 569
TspDTI ATGAA 1 cut(s) 581
TspGWI ACGGA 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 2 cut(s) 385, 661
XceI RCATGY 1 cut(s) 534
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.